Microbiological Quality and Safety of Two Popularly Consumed Raw, Street Vended Foods in India

Abhijit Ganguli,Sunita Bansal,Namita Malik,Arun Kumar Rana,Moushumi Ghosh 한국식품과학회 2004 Food Science and Biotechnology Vol.13 No.4

Evidence of Indigenous NAH Plasmid of Naphthalene Degrading Pseudomonas putida PpG7 Strain Implicated in Limonin Degradation

Moushumi Ghosh,Abhijit Ganguli,Meenakshi Mallik 한국미생물학회 2006 The journal of microbiology Vol.44 No.5

A well characterized naphthalene-degrading strain, Pseudomonas putida PpG7 was observed to utilize limonin, a highly-oxygenated triterpenoid compound as a sole source of carbon and energy. Limonin concentrations evidenced a 64% reduction over 48 h of growth in batch cultures. Attempts were made to acquire a plasmid-less derivative via various methods (viz. Ethidium Bromide, SDS, elevated temperature & mitomycin C), among which the method involving mitomycin C (20 μg/ml) proved successful. Concomitant with the loss of plasmid in P. putida PpG7 strain, the cured derivative was identified as a lim- phenotype. The lim+ phenotype could be conjugally transferred to the cured derivative. Based on the results of curing with mitomycin C, conjugation studies and presence of ndo gene encoding naphthalene 1,2 dioxygenase, it was demonstrated that genes for the limonin utilization were encoded on an 83 kb indigenous transmissible Inc. P9 NAH plasmid in Pseudomonas putida PpG7 strain.

DNA Polymorphism in SLC11A1 Gene and its Association with Brucellosis Resistance in Indian Zebu (Bos indicus) and Crossbred (Bos indicus×Bos taurus) Cattle

Kumar, Nishant,Ganguly, Indrajit,Singh, Rajendra,Deb, Sitangsu M.,Kumar, Subodh,Sharma, Arjava,Mitra, Abhijit Asian Australasian Association of Animal Productio 2011 Animal Bioscience Vol.24 No.7

The PCR- restriction fragment length polymorphism (RFLP) in and around TM4 of SLC11A1 gene and its association with the incidences of brucellosis in Hariana breed (Bos indicus) and Holstein Friesian crossbred (Bos indicus${\times}$Bos taurus) cattle was examined. A fragment of 954 bp encoding the TM4 was amplified, and RFLP was identified by digestion of the amplicon independently with AluI and TaqI. The amplicon (GenBank Acc. No. AY338470 and AY338471) comprised of a part of exon V (<59 bp) and VII (62>), and entire intron 5 (423 bp), exon VI (71 bp) and intron 6 (339 bp). Digestion with AluI revealed the presence of two alleles viz, A (281, 255, 79 and 51 bp) and B (541, 255, 79 and 51 bp). The frequency of A allele was estimated as 0.80 and 0.73 in Hariana and crossbred cattle, respectively. Due to presence of a polymorphic TaqI site at intron 5, two alleles: T (552 and 402 bp) and Q (231, 321 and 402 bp) were identified. The frequency of T allele was estimated as 0.96 and 0.97, respectively. For association study, on the basis of serological tests and history of abortion, the animals were grouped into "affected" and "non-affected". However, no association could be established with the observed RFLPs.

Evidence of Indigenous NAB Plasmid of Naphthalene Degrading Pseudomonas putida PpG7 Strain Implicated in Limonin Degradation

Ghosh, Moushumi,Ganguli, Abhijit,Mallik, Meenakshi The Microbiological Society of Korea 2006 The journal of microbiology Vol.44 No.5

A well characterized naphthalene-degrading strain, Pseudomonas putida PpG7 was observed to utilize limonin, a highly-oxygenated triterpenoid compound as a sole source of carbon and energy. Limonin concentrations evidenced a 64% reduction over 48 h of growth in batch cultures. Attempts were made to acquire a plasmid-less derivative via various methods (viz. Ethidium Bromide, SDS, elevated temperature & mitomycin C), among which the method involving mitomycin C ($20{\mu}g/ml$) proved successful. Concomitant with the loss of plasmid in P. putida PpG7 strain, the cured derivative was identified as a $lim^-$ phenotype. The $lim^+$ phenotype could be conjugally transferred to the cured derivative. Based on the results of curing with mitomycin C, conjugation studies and presence of ndo gene encoding naphthalene 1,2 dioxygenase, it was demonstrated that genes for the limonin utilization were encoded on an 83 kb indigenous transmissible Inc. P9 NAH plasmid in Pseudomonas putida PpG7 strain.

Static and dynamic analysis of cable-suspended concrete beams

Pankaj Kumar,Abhijit Ganguli,Gurmail Benipal 국제구조공학회 2017 Structural Engineering and Mechanics, An Int'l Jou Vol.64 No.5

A new theory of weightless sagging planer elasto-flexible cables under point loads is developed earlier by the authors and used for predicting the nonlinear dynamic response of cable-suspended linear elastic beams. However, this theory is not valid for nonlinear elastic cracked concrete beams possessing different positive and negative flexural rigidity. In the present paper, the flexural response of simply supported cracked concrete beams suspended from cables by two hangers is presented. Following a procedure established earlier, rate-type constitutive equations and third order nonlinear differential equations of motion for the structures undergoing small elastic displacements are derived. Upon general quasi-static loading, negative nodal forces, moments and support reactions may be introduced in the cable-suspended concrete beams and linear modal frequencies may abruptly change. Subharmonic resonances are predicted under harmonic loading. Uncoupling of the nodal response is proposed as a more general criterion of crossover phenomenon. Significance of the bilinearity ratio of the concrete beam and elasto-configurational displacements of the cable for the structural response is brought out. The relevance of the proposed theory for the analysis and the design of the cable-suspended bridges is critically evaluated.

Statistical Optimization of Physical Parameters for Enhanced Bacteriocin Production by L. casei

Mukesh Kumar,Alok Kumar Jain,Moushumi Ghosh,Abhijit Ganguli 한국생물공학회 2012 Biotechnology and Bioprocess Engineering Vol.17 No.3

Antimicrobial proteinaceous compounds such as bacteriocins produced from Lactobacillus sp. are widely known. They have potential antimicrobial activities towards closely related bacteria and several pathogens associated with food spoilage and hence can be a potential food bio-preservative agent. Bacteriocin production requires optimized process, complex media and well-controlled physical conditions including pH and temperature. A probiotic strain of L. casei LA-1 isolated from mango pickle was used in the present study. The influence of physical parameters viz. temperature (15 ~ 45oC), pH (4.0 ~7.0), incubation time (up to 48 h) and inoculum size (0.7 ~2.0 O.D) on bacteriocin production was analyzed. The effect of all the parameters was first investigated using the one-factor-at-a-time method (OFAT) to see the significance of these parameters on bacteriocin production and then further optimized by response surface methodology (RSM). Following OFAT analysis, all factors were found to have a significant effect on bacteriocin production. Bacteriocin production of 2,844 AU/mL was obtained at temperature 37oC, pH 6.7 and inoculum size 1.8 O.D at an incubation time of 20 h and it was produced during the stationary phase of growth. Statistical analysis showed that three variables-pH, temperature and incubation time have significant effects on bacteriocin production. RSM proved to be a powerful tool in the optimization of bacteriocin production by L. casei LA-1 with a two-fold increase,giving a production of 4652.15 AU/mL at pH 7.19, temperature 33.3oC and incubation time of 22.2 h.

Survival of Probiotic Strains in Non-dairy Indian Spice Condiment Exhibiting Cholesterol Reducing Properties

Mukesh Kumar Singh,Richu Singla,Arashdeep Singh,Moushumi Ghosh,Abhijit Ganguli 한국식품과학회 2012 Food Science and Biotechnology Vol.21 No.5

The feasibility of a novel formulated spice condiment suitable for delivery of probiotic cultures to consumers has been reported in this study. The spice condiment (pH 4.5, Aw=0.81) exhibited high sensorial scores and stability after storage for 60 days. Lactobacillus casei and Lactobacillus delbrueckii strains were incorporated in the spice condiment and evaluated for survival and expression of important functional characteristics: cholesterol reduction. Both strains survived excellently in the food matrix and sustained simulated gastro-intestinal conditions (viability to 0.3% bile salts and pancreatic enzymes). Probiotic enriched spice condiment fed to experimental mice on high cholesterol diet resulted in significant (p<0.05) lowering of serum total cholesterol, HDLs,LDLs, and triglycerides; moreover; histopathological changes were less intensive. Results of our study suggested the formulated spice condiment as a potential non-dairy food adjunct for delivery of probiotics and may help in managing hypercholesterolaemia.