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돼지편층의 45kDa 항원단백질에 대한 단클론항체 생산
이종경,김종태,서흔수,박종열,윤희정,Lee, Jong-Kyung,Kim, Jung-Tae,Seo, Hun-Su,Park, Jong-Yeol,Yun, Hee-Jeong 대한수의학회 2004 大韓獸醫學會誌 Vol.44 No.4
Trichnuris suis does not excrete eggs during larval stage as well as in particular adult stage, It is impossible to diagnose by use of fecal examination method in those periods. Therefore, serological diagnostic method can be very useful for those stages. In order to produce monoclonal antibody, specific somatic and secretory-excretory (SE) antigens of T. suis were identified and analyzed by SDS-PAGE and Western blot. Monoclonal antibody-producing hybridoma cells were cloned, which were made of popliteal lymph node of BALB/c mice immunized with a 45 kDa somatic antigen of T. suis. Five clones (1B9, 2C4, n2C5, 2D7 and 2D8) showing strong responses to T. suis antigens were selected and the isotype identified. All monoclonal antibodies were IgG1 isotype and the light chains were k chain. Established monoclonal antibodies reacted specifically to somatic and SE antigens of T. suis and did not cross-reacted to antigens of ascaris suum, trichuris vulpis, or Trichinella spiralis. The sensitivity of somatic and SE antigens against these monoclonal antibodies were significant (p<0.01) associated with those of positive and negative sera.
이종경 ( Jong Kyung Lee ) 한국급식외식위생학회 2023 급식외식위생학회지 Vol.4 No.2
The FoodTech industry has been developed with the rise of start-up by using AI, big data, robotics, biotechnology. In addition, sustainable development is more important with the trend of population growth, aging, and climate change. We investigated the impact of FoodTech on the foodservice industry with the cases of the global and domestic companies. The technology of AI, IoT, blockchain, robotics, automation systems are widely used to improve food safety and hygiene while as the use of diagnostic biomarkers such as blood or DNA, digital platform and app, and AI-based solutions are used in the field of personalized nutrition. With the expand of FoodTech in foodservice industry, the competencies that the managers need to develop include understanding technology, resource management, self-development, work ethics, problem-solving, and communication, therefore the support of the related education and training is required.