http://chineseinput.net/에서 pinyin(병음)방식으로 중국어를 변환할 수 있습니다.
변환된 중국어를 복사하여 사용하시면 됩니다.
김수언,김인수,Kim, Soo-Un,Kim, In-Soo,Anderson, John A. 생화학분자생물학회 1985 한국생화학회지 Vol.18 No.3
Claviceps, purpurea PRL 1980의 microsome에는 단백질 mg 당 40 pmole의 cytochrome P-450이 함유되어 있었다. 이 microsome으로부터 단백질 mg 당 420 pmole의 cytochrome P-450 및 387 pmole의 NADPH-cytochrome c reductase로 정제하였다. 부분 정제된 이 두 단백질로 재구성을 시도하였을 때 agroclavine hydroxylase 활성을 나타내지 않았으나 NADPH-cytochrome c reductase를 microsome에 첨가하였을 경우에는 그 활성이 80% 증가되었다. Claviceps sp. SD58의 microsome에 있는 agroclavine hydroxylase의 $V_{max}$와 $K_m$값은 agroclavine에 대하여 각각 3.3 pkat와 0.11 mM 이었다. 또 이 균으로부터 정제된 cytochrome P-450과 기질인 agroclavine의 복합체는 제2특성을 갖는 spectrum을 나타내었다. 또 위의 두 균주로 부터 분리한 cytochrome P-450이 이산화탄소와 결합할때 그 difference spectrum의 최대흡광은 449 nm 이었다. Microsomes of Claviceps purpurea PRL 1980 had a maximum concentration of cytochrome P-450 of 40 pmole per mg protein. Cytochrome P-450 and NADPH-cy-to chrome c reductase were purified from C. purpurea PRL 1980 microsomes to concentrations of 421 pmole per mg protein and 387 pmole per min per mg protein, respectively. Activity was not reconstituted from these fractions, but NADPH-cytochrome c reductase increased agroclavine hydroxylase activity of C. purpurea PRL 1980 microsomes. The activity was not stimulated by rat liver microsomal NADPH -cytochrome c reductase. Claviceps sp. SD58 microsomal agroclavine hydroxylase had $V_{max}$ of 3.3 pkat per mg and $K_m$ of 0.11 mM for agroclavine. Agroclavine bound to cytochrome P-450 from Claviceps sp. SD58 to give a type II spectrum. The absorbance maximum in the CO-reduced vs reduced difference spectrum of purified cytochrome P-450 from C. purpurea PRL 1980 and Claviceps sp. SD58 was at 449 nm.
Claviceps sp . 에서 Cytochrome P - 450 및 NADPH - Cytochrome c Reductase 정제 및 그 특성
김수언,김인수,John A . Anderson ( Soo Un Kim,In Soo Kim,John A . Anderson ) 생화학분자생물학회 1985 BMB Reports Vol.18 No.3
Microsomes of Claviceps purpurea PRL 1980 had a maximum concentration of cytochrome P-450 of 40 pmole per ㎎ protein. Cytochrome P-450 and NADPH-cytochrome c reductase were purified from C. purpurea PRL 1980 microsomes to concentrations of 421 pmole per ㎎ protein and 387 pmole per min per mg protein, respectively. Activity was not reconstituted from these fractions, but NADPH-cytochrome c reductase increased agroclavine hydroxylase activity of C. purpurea PRL 1980 microsomes. The activity was not stimulated by rat liver microsomal NADPH-cytochrome c reductase. Claviceps sp. SD58 microsomal agroclavine hydroxylase had V_(max) of 3.3 pkat per ㎎ and K_m of 0.11 mM for agroclavine. Agroclavine bound to cytochrome P-450 from Claviceps sp. SD58 to give a type II spectrum. The absorbance maximum in the CO-reduced vs reduced difference spectrum of purified cytochrome P-450 from C. purpurea PRL 1980 and Claviceps sp. SD58 was at 449 ㎚.
김미선(Mi Sun Kim),김수언(Su Un Kim),정삼주(Sam Ju Jung),김영은(Young Eun Kim),김민정(Min Jung Kim),이명숙(Myung Sook Lee),황인숙(In Sook Hwang) 한국환경보건학회 2023 한국환경보건학회지 Vol.49 No.2
Background: Along with the increase in consumer interest in and consumption of tattoo products, the controversy over harmful heavy metals associated with the use of tattoo cosmetics is also increasing. Therefore, investigation of hazardous metals in these tattoo products is needed. Objectives: This study was performed to provide useful data for establishing reasonable standards to securely manage tattoo cosmetics, tattoo stickers, and tattoo inks distributed in the market. Methods: Thirteen kinds of hazardous metal contents (Pb, As, Cd, Sb, Ni, Co, Cu, Cr, Se, Ba, Zn, Sn, and Hg) were analyzed for 23 tattoo cosmetics, ten tattoo stickers, and 16 tattoo inks. Hg was measured through the combustion-gold amalgamation method, and other hazardous metals were measured by inductively coupled plasma-mass spectrometry (ICP-MS) after acidic decomposition using a microwave apparatus. Results: The detected ranges of Pb, As, Cd, Sb, Ni, and Hg in tattoo cosmetics were 0.07~1.18, 0.06~0.41, ND~0.07, 0.01~3.44, 0.12~2.75, and ND~0.01 μg/g, respectively. All of the hazardous metals detected were below the recommended maximum standards of the Ministry of Food and Drug Safety. The mean amount of Pb detected in tattoo stickers for children was 0.24 μg/kg and Cd was not detected, meaning both metals met the recommended criteria. There was no statistically significant difference in all measured metals between children’s tattoo stickers and adults’ tattoo stickers. In the results of the study on the hazardous metal content of tattoo inks, four products (25%) for Pb, one product (6%) for As, 13 products (81%) for Ni, four products (25%) for Cu, and five products (31%) for Zn exceeded the recommended standards approved by the government. The highest predicted exposure amount for hazardous metals exceeding the recommended level of tattoo inks in a single tattooing was 5.69 μg/kg for Ni, 8.51 μg/kg for Zn, 0.44 μg/kg for Pb, 8.07 μg/kg for Cu, 0.44 μg/kg for As, and 71.36 μg/kg for Ba. Conclusions: It is necessary to prepare criteria for content limitation for the management of Co, Cr, Ba and Se tattoo cosmetics, and tattoo inks require thorough quality control.
갈화 ( Puerariae flos ) 추출물이 Rat 혈중 Ethanol 농도에 미치는 영향
김정한(Jeong Han Kim),민선식(Sun Sik Min),김성훈(Sung Hoon Kim),홍희도(Heu Do Hong),김종수(Jong Soo Kim),김수언(Soo Un Kim) 한국응용생명화학회 1995 Applied Biological Chemistry (Appl Biol Chem) Vol.38 No.6
Ethanol concentration in blood, brain and liver of rats was shown to be effectively lowered by arrowroot flower extract. The lowering effect for ethanol concentration in blood was maximum when measured after 1 hour from ethanol feeding. Hot water extract was more effective than 80% ethanol extract. The treatment of extract at 10 min. before ethanol feeding gave a better result than that at 10 min after or 1 hour before ethanol feeding. The ethanol concentration in brain and liver was lowered as found in the blood ethanol concentration. Acetaldehyde was not detected either in blood or the tissues. The optimal amount of the Puerariae flos was 55.7㎎/㎏·body weight. The newly developed analytical method using dichloromethane as extracting solvent was proven to be very effective in terms of speed and simplicity.
유통 두족류의 중금속(Hg, Pb, Cd, Cu) 함량과 위해성 평가
김수언(Su-Un Kim),황영옥(Young-Ok Hwang),박애숙(Ae-Sook Park),박영애(Young-Ae Park),함희진(Hee-Jin Ham),최성민(Sung-Min Choi),김정헌(Jung-Hun Kim) 한국식품영양과학회 2011 한국식품영양과학회지 Vol.40 No.4
본 연구에서는 저서성 두족류 낙지를 중심으로 주꾸미, 문어, 오징어 144건에 대해 생체에 강한 축적 경향으로 식품안전상 중요시 되는 수은, 납, 카드뮴, 구리의 중금속 함량을 분석하였다. 두족류 가식부의 중금속 함량은 수은 0.017±0.063 mg/kg, 납 0.024±0.004 mg/kg, 카드뮴 0.030±0.027 mg/kg, 구리 2.536±0.653 mg/kg이었으며, 내장에서는 수은 0.063±0.016 mg/kg, 납 0.579±0.304 mg/kg, 카드뮴 15.200±6.189 mg/kg, 구리 201.706±129.7 mg/kg이었다. 낙지 가식부와 내장의 비로 중금속의 축적정도를 파악해보면, 카드뮴이 가장 높았고 다음이 구리, 납, 수은 순이었으며, 낙지 무게에 따른 중금속의 축적 정도와는 관련이 없는 것으로 나타났다. 또한 우리나라 국민이 낙지를 통해 섭취하는 수은, 납, 카드뮴, 구리 등의 중금속 주간 섭취량은 FAO/WTO에서 중금속 안전성 평가를 위해 정한 잠정주간섭취허용량인 PTWI의 0.0~0.20% 수준으로 안전한 것으로 판단되며, 비가식부(내장)의 경우는 0.05~92.28%로 높게 나타나 낙지 섭취 시 낙지 내장을 제거한 후 섭취하는 것이 안전할 것으로 판단된다. The purpose of this study was to investigate certain heavy metals (Hg, Pb, Cd, Cu) in 144 samples of cephalopods, to identify weekly heavy metal intakes and to evaluate potential health risks. The average concentrations in the arm, expressed in mg/kg, were: Hg 0.017 (less than the limit of detection (LOD) to 0.059), Pb 0.024 (less than the LOD to 0.092), Cd 0.030 (less than the LOD to 0.627) and Cu 2.536 (less than the LOD to 5.837). The average concentration in the internal organs, expressed in mg/kg, were: Hg 0.063 (from 0.008 to 0.543), Pb 0.579 (less than the LOD to 2.344), Cd 15.200 (from 0.654 to 75.29) and Cu 201.706 (from 2.412 to 856.4). Heavy metal concentrations were higher in the organs than in the arm. Of the four heavy metals, the ratio of internal organs to arm was highest for cadmium. The weekly intakes of Hg, Pb, Cd and Cu from Octopus minor were 0.2%, 0.08%, 0.20% and 0.00%, respectively from the arm, and 1.0%, 0.96%, 92.28% and 0.05% from the internal organs as compared to PTWI (provisional tolerable weekly intakes) established by the FAO/WHO Expert Committee for Food Safety Evaluation.