4. 종자 및 배지상에서의 Didymella bryoniae (오이류 덩굴마름병균) 의 형태적 및 배양적 특징

이두형 한국균학회 1981 韓國菌學會誌 Vol.9 No.4

Habit characteristics of imperfect and perfect stage of D. bryoniae encountered on naturally infected seeds of cucumber and pumpkin were studied by the blotter method and compared with those grown on Difco potato dextrose agar, v-8 juice agar and Water Agar Leaf Medium. Most of the pycnidiospores obtained from each isolate of this fungus grown on PDA were nonseptate and microtype. Non-septate pycnidiospores were predominanted in all isolates, but a macrotype of the non-septate and a number of uniseptate pycnidiospores were produced on V-8 juice agar and Water Agar Leaf Medium. On seed the pycnidiospores were mostly nonseptate, but rarely uniseptate ones were also found. On radicle of cucumber seed, the pycnidiospores were non-septate and uniseptate but small percentage biseptate with somewhat constricted at septa. Pycnidiospores produced on V-8 juice agar and Water Agar Leaf Medium were similar to those produced on seeds. In the present investigation the perithecia were mostly globose to subglobose with apical papillate ostiole and whitish spore masses formed on the ostiole of perithecia, either on naturally infected seed or on culture media. The mature perithecia were dark brown to black. They were partially embedded or erumpent on seed coat and culture media. The perithecia varied in size within a much narrower range than the pycnidia. But perithecial formation of this fungus on PDA, v-8 juice agar, WALM and seed varied considerably depending upon isolate and substrate.

발생이 늘고있는 과수병해의 특징과 방제대책 - (1) 사과나무

이두형 한국작물보호협회 1992 자연과 농업 Vol.13 No.2

과수의 병해방제와 살균제의 효과적인 사용법

이두형 한국작물보호협회 1984 자연과 농업 Vol.5 No.8

우리나라에 제일 먼저 도입된 과수는 사과이며 그후 경제적인 과수원이 조성된것은 1901년이고 국가적인 차원에서 시험연구사업이 시작된 것은 1906년부터이다. 과수병의 연구는 1910년 부터 배나무 붉은별무늬병을 비롯하여 몇가지 병에 대한 약제방제시험으로부터 시작되었다. 본격적인 농약으로서의 살균제는 석회보르도액이며 1945년 이전까지 주로 과수원에서만 사용되어 왔다.

포도의 문제병은 무엇인가? - 탄저병, 갈색무늬병, 노균병, 새눈무늬병, 흰가루병

이두형 한국작물보호협회 1987 자연과 농업 Vol.8 No.4

포도나무에 발생하는 병(病)은 현재 19종(1986)이 알려져 있으며, 병 발생의 중요도가 여러 가지 요인의 변화에 따라서 바뀌고 있다. 그 요인을 우선 약제사용면에서 보면 약제종류의 변화, 살포방법의 변화, 살포회수의 변화 및 포장위생의 불철저 등을 들 수 있고 재배적인 면에서 보면 품종의 변천, 재배형의 변화, 시비방법의 변화 등을 들 수 있다. 또 해와 지역에 따라서는 이상저온, 다우다습(多雨多濕), 건조 등 기상 조건이 변함에 따라서도 병의 발생은 크게 달라 진다.

A review of biology and management of Lycorma delicatula (Hemiptera: Fulgoridae), an emerging global invasive species

이두형,박용락,Tracy C. Leskey 한국응용곤충학회 2019 Journal of Asia-Pacific Entomology Vol.22 No.2

Lycorma delicatula (White) (Hemiptera: Fulgoridae) is an invasive species inflicting damage to agricultural and forest systems. This species is native to China, Taiwan, and Vietnam. Lycorma delicatula was accidentally introduced into South Korea and Japan, with outbreaks reported since mid-2000s. More recently, this pest was found in Pennsylvania, USA, and its geographical distribution has steadily expanded since the introduction with increasing population densities in the USA. This species is univoltine in invaded regions and overwinters in the egg stage. Lycorma delicatula is known to attack at least 65 host plants, and so-called preferred host plants such as Ailanthus altissima and Vitis vinifera are present in all invaded countries. This species threatens grape industries in China, South Korea, and USA, with the impact on other crops still unknown, at least in the USA. For monitoring this pest, light or pheromone traps have not yet been developed, but using sticky tree bands may serve as a promising tool. To date, visual survey is the primary means for detecting L. delicatula and studying their population dynamics. To control L. delicatula, several studies evaluated conventional insecticides. Under field conditions, chlorpyrifos, dinotefuran, etofenprox, and etofenprox + diazinon yielded high levels of mortality against this pest. In general, environmentally-friendly materials, registered as Environmentally-Friendly Agricultural Material in South Korea, resulted in lower insecticidal efficacy against L. delicatula compared with conventional chemicals. Natural enemies of L. delicatula such as egg parasitoids in the genus Anastatus and Dryinus are promising candidates as a long-term solution for management.

은행나무의 잎끝마름병에 미치는 SO2 의 영향

이두형,배공영,박소홍,강연지 한국환경농학회 1999 한국환경농학회지 Vol.18 No.2

대기오염과 병충해발생과의 관계를 규명하고자 은행나무에 SO₂가스를 처리한 후, 잎끝마름병균을 접종하여 식물의 생리·생화학적인 변화를 조사하였다. 0.1㎕/ℓ의 SO₂가스를 하루 8시간씩 7일간 은행나무에 처리한 결과 Chlorosis등의 가시피해는 나타나지 않았으나, 광합성이 대조구에 비해 40%가량 감소되고, CO₂이용효율과 수분이용효율이 감소되는 현상을 보였다. 가스처리 후 병원균을 상처접종시키고 42일 뒤 SI와 SFI의 발병도를 조사한 결과 SI가 SFI에 비해 3배 가량 빠르게 병이 진전되어 있었다. 병원균의 먹이가 되는 당의 함량은 대조구, SFI, SI간에 유의성을 보일 만큼의 차이는 보이지 않았으나, 광합성은 SI가 SFI에 비해 11%가량 저하되었다. 광합성이 저하되었음에도 불구하고 당의 함량이 유지되었으므로 탄소 고정관계를 알아본 결과 CO₂ 이용효율이 저하되지 않았음을 알 수 있었다. 이와 함께 수분이용효율이 SI에서 SFI에 비해 13.2% 증가되었다. 이상의 결과를 종합해 보면, 이온유출량 등으로 보아 SO₂가스처리로 인해 식물이 연약해져 병원균에 대한 저항력이 낮아졌고, 수분이용효율의 증가로 인해 탄소 고정이 활발하게 이루어짐으로써 당이 원활히 합성되는 한편 내부적인 변화로 인해 합성된 당의 전류가 억제되어 잎에 당이 축적되었기 때문에 SI의 발병도가 훨씬 높았던 것으로 생각된다. To examine the relationship between air pollution and occurrence of disease in plants, we investigated the alteration of physiology and biochemistry of Ginkgo biloba by inoculating with Coniothyrium sp. and fumigating with 0.1㎕/ℓSO_(-2) Visual damage did not appear but photosynthesis, CO₂use efficiency(CUE) and water use efficiency(WUE) were reduced when G. biloba was exposed to SO₂for 7days (8 hours a day). When inoculated with Coniothyrium sp., the seventies of damage in G. biloba showed a threefold difference between SO₂-treatment (SI) and SO₂-free treatment (SFI) at day 42 from initial inoculation. Little difference was observed in sugar contents that may be used pathogens feed, among control, SFI and SI. In spite of the reduction in photosynthetic rate, sugar contents and CUE were maintained. WUE was enhanced 13% more at SI than SFI. The CO₂fixation boosted because of enhanced WUE, and thus sugar synthesis was not affected. In addition, sugar transport seems to be retarded for some internal alteration. Consequently, the severity of SI was more serious than that of SFI because Coniothyrium sp. easily invades into the physical texture of G. biloba weakened by SO₂fumigation and because sugar was accumulated in leaves of G. biloba.

살균제의 작용기구와 사용시 주의점 (5) - 흡수약제성분의 균체축적여부가 중요

이두형 한국작물보호협회 1982 자연과 농업 Vol.3 No.3

초기 족관절 골관절염 환자에서의 히알루론산 관절강 내 주사 요법

이두형,김태훈,한승환,Lee, Doo-Hyung,Kim, Tae-Hun,Han, Seung-Hwan 대한족부족관절학회 2011 대한족부족관절학회지 Vol.15 No.1

Purpose: This study evaluated the efficacy of an intra-articular injection of sodium hyaluronate in the treatment of early stage osteoarthritis of the ankle. Materials and Methods: Early stage ankle osteoarthritis (Takakura stage I and II) patients who received hyaluronic acid injection therapy were retrospectively reviewed. Patients underwent intra-articular injections of 2 mL of sodium hyaluronate for 3 consecutive weeks. Clinical evaluation and Visual Analogue Scale (VAS) scoring were performed at every three months after treatment. Results: Total 27 patients were involved and mean age was 55 year old (range 33 to 77 years). There were 13 male and 14 female patients. Mean follow up duration was 13 month and. Pre-intra-articular VAS score was $8.9{\pm}0.7$ and three month follow up score was $3.8{\pm}2.8$. VAS score of last follow up was $3.2{\pm}3.4$. The effect of hyaluronic acid continued about one year when analyzed the VAS score change of the patients followed for more than one year. Patients' satisfaction was "very satisfied" in nine, "satisfied" in 12, "fair" in one, and "not satisfied" in five patients. Overall satisfaction rate was 82%. There were no ankle osteoarthritis stage changes in serial follow up radiograph. Conclusion: Symptomatic relief of signs and symptoms of osteoarthritis of the ankle was achieved by injection of an intra-articular hyaluronate injection. Efficacy of Hyaluronate acid injection persisted more than 1 year in our study. Intra-articular hyaluronate injection to ankle osteoarthritis is safe and effective as knee joint and should be considered as a valid conservative treatment for ankle osteoarthritis.

가스분사법으로 제조된 Mg-6 wt.% Al-1 wt.% Zn 합금의 시효특성

이두형,김보식,장시영,Lee, Du-Hyung,Kim, Bo-Sik,Chang, Si-Young 한국분말야금학회 2009 한국분말재료학회지 (KPMI) Vol.16 No.4

The aging characteristics of gas atomized Mg-6 wt.% Al-1 wt.% Zn alloy were investigated and compared to those of cast Mg-6 wt.% Al alloy. The gas atomized Mg-6 wt.% Al-1wt.% Zn alloy powders had spherical morphology between 1 and 100 $\mu m$ in diameter. After compaction under the pressure of 700 MPa at $320^{\circ}C$ for 10 min, the Mg-6 wt.% Al-1 wt.% Zn alloy showed a grain size of approximately 40 $\mu m$ which is smaller than that of the cast Mg-6 wt.% Al alloy, and a relative compact density of approximately 93%. After ageing, the Mg-6 wt.% Al-1 wt.% Zn alloy showed much faster peak hardness than cast Mg-6 wt.% Al alloy. The Mg-6 wt.% Al-1 wt.% Zn alloy showed the new fine precipitations with ageing time, while the cast Mg-6 wt.% Al alloy was almost similar morphology.

피막처리 시간에 따른 Mg-Al 합금의 플라즈마 전해 산화 피막 특성

이두형,김보식,장시영,Lee, Du-Hyung,Kim, Bo-Sik,Chang, Si-Young 한국재료학회 2008 한국재료학회지 Vol.18 No.5

Pure Mg and Mg-6wt.%Al alloy were coated by the plasma electrolytic oxidation with various coating times and the microstructural and mechanical characteristics of the coatings were investigated. The coatings on pure Mg and Mg-6wt.%Al alloy consisted of MgO and $Mg_2SiO_4$. The surface roughness and thickness of the coatings became larger as the coating time increased. The coatings on the Mg-6wt.%Al alloy were more uniform and thicker than those on pure Mg. The microhardness and friction coefficient of the coatings increased progressively as the coating time increased. In addition, the coatings on the Mg-6wt.%Al alloy compared to pure Mg showed improved microhardness and a better friction coefficient.