Poloxamer-407로 유도한 이상지질혈증 생쥐에서 홍삼의 예방 효과

홍성실(Sung-Sil Hong),이진우(Jin-Woo Lee),박윤아(Yun-A Park),김성혁(Sung-Hyeok Kim),장소희(So-Hee Jang),하창우(Chang-Woo Ha),김미자(Mi-Ja Kim),남궁승(Seung Namkoong),구현정(Hyun-Jung Koo),손은화(Eun-Hwa Sohn) 한국산학기술학회 2021 한국산학기술학회논문지 Vol.22 No.10

본 연구에서는 홍삼의 이상지질혈증 개선 효능과 안전성을 조사하고자 하였다. Poloxamer-407(P407)에 의한 이상지질혈증을 유발한 동물모델에 홍삼추출물과 지질강하제 simvastatin을 2주간 복강 투여한 후 지질프로파일과 간독성의 조절효과를 확인하였다. 실험동물은 8주령 수컷 ICR 마우스(n = 30)를 6개 그룹(무처치군, P407 유도군과 홍삼추출물 10, 50, 100 mg/kg 투여군 및 simvastatin 투여군)으로 나누어 각 그룹 당 5마리씩 무작위로 배치하였다. 실험종료 후 각 그룹의 마우스에서 간, 부고환 지방, 흉선 및 비장을 적출 하여 중량을 측정하였다. 또한 혈액에서 총콜레스테롤, 중성지방, 저밀도지단백질, 고밀도지단백질을 포함한 지질프로파일의 변화와 glutamate oxaloacetate transaminase(GOT) 및 glutamate pyruvate transaminase(GPT)를 측정하였다. 실험결과 홍삼추출물 10 mg/kg 투여군에서 P407로 증가된 혈중 총콜레스테롤, 중성지방, 저밀도지단백질을 모두 유의적으로 감소시켰으며(p <0.05), 그 효과는 simvastatin 10 mg/kg 투여군과 유사한 수준으로 나타났다. 또한, 홍삼추출물은 혈중 GPT와 GOT 수치에 영향을 미치지 않았으며 주요면역기관인 흉선의 무게를 증가시켰다. 이러한 결과는 홍삼이 이상지질혈증에 예방 효과가 있고 지질관련질환에 대한 statin계열 약물의 보조물질로서 활용 가능성을 제시한다. This study aimed at investigating the efficacy and safety of red ginseng (RG) for improving dyslipidemia. After 2 weeks of intraperitoneal administration of RG and simvastatin (lipid-lowering medication) to an animal model with dyslipidemia induced by poloxamer-407 (P407), the regulatory effects on the lipid profile and hepatotoxicity were confirmed. 8-week-old male ICR mice (n = 30) were divided into 6 groups (control, P407-induced dyslipidemia group, RG 10, 50, 100 mg/kg group, simvastatin group). 5 mice were randomly placed in each group. At the end of the experiment, the weight of the liver, epididymal fat, thymus, and spleen was measured in each group. We also identified changes in the lipid profile, including total cholesterol (TC), triglycerides (TG), low-density lipoproteins (LDL), high-density lipoproteins (HDL), and determined the levels of the glutamate oxaloacetate transaminase (GOT) and glutamate pyruvate transaminase (GPT) in the blood. The results show that 10 mg/kg RG significantly reduced the increase of P407-induced levels of TC, TG, and LDL (p <0.05), similar to the simvastatin group. Moreover, RG did not affect the levels of GPT and GOT, and increased the weight of the thymus, a major immune organ. These results suggested that RG may be able to prevent dyslipidemia and has therapeutic potential as an adjuvant to statins for the management of lipid disorders.

간호대학생의 임상실습 스트레스와 무력감이 간호전문직관에 미치는 영향

홍성실(Sungsil Hong),박미정(MiJeong Park) 한국콘텐츠학회 2014 한국콘텐츠학회논문지 Vol.14 No.12

본 연구는 간호대학생이 임상실습 시 경험하는 임상실습 스트레스와 무력감이 간호전문직관에 미치는 영향력을 확인하기 위한 서술적 조사연구이다. 본 연구는 2014년 6월 23일부터 7월 11일까지 S시와 C시의 4개 대학 간호학과 3, 4학년에 재학 중인 205명의 간호대학생을 대상으로 구조화된 질문지를 이용하여 진행되었으며, 수집된 자료는 t-test, One-way ANOVA, Pearsons correlation coefficient, Hierarchical multiple regression 등을 통해 분석되었다. 연구 결과는 다음과 같다. 연구 대상자의 간호전문직관은 점수가능범위 1~5점 중 3.56점으로 비교적 높았으며, 전공만족도와 임상실습 만족도가 높을수록 간호전문직관이 유의하게 높게 나타났다. 둘째, 임상실습 스트레스(p<.001)와 무력감(p<.001)은 간호전문직관과 유의한 음의 상관관계를 나타냈다. 셋째, 전공만족도, 임상실습 만족도, 임상실습 스트레스, 무력감을 모두 포함한 예측 모형의 설명력은 22.1%였고(p<.001), 전공만족도, 임상실습 스트레스와 무력감이 영향을 미치는 주요 변인으로 확인되었다. 본 연구 결과가 임상실습으로 인한 간호대학생의 간호전문직관 변화에 대한 이해를 높이고, 이들의 간호전문직관 향상을 위한 교육 프로그램 개발 시 임상실습 스트레스와 무력감의 감소시킬 수 있는 중재 전략의 기초자료로서 활용될 수 있기를 기대한다. This study was conducted as a descriptive survey for understanding the effects of clinical practice stress and helplessness experienced by nursing students during clinical practice on their nursing professionalism. The survey was conducted using a structured questionnaire with 205 junior and senior nursing students from four universities in S and C Cities during the period from June 23 to July 11, 2014, and collected data were analyzed through t-test, One-way ANOVA, Pearsons correlation coefficient, and hierarchical multiple regression. According to the results, first, the score for subjects nursing professionalism was 3.56 on 1 to 5 point scale. Their nursing professionalism was higher major satisfaction and clinical practice satisfaction. Second, significant negative correlations with nursing professionalism were shown by clinical practice stress (p<.001) and helplessness (p<.001). Third, the explanatory power of the predictive model involving major satisfaction, clinical practice satisfaction, clinical practice stress, and helplessness was 22.1% (p<.001), and the subjects major satisfaction, clinical practice stress, and helplessness were identified as main factors having influence it. The results of this study are expected to be used as basic data for development of education programs to reduce clinical practice stress and helplessness experienced by nursing students during clinical practice.

담배 흡연이 생쥐의 흉선 및 비장의 면역 기능에 미치는 영향

홍성실(Sung-Sil Hong),박윤아(Yun-A Park),이진우(Jin-Woo Lee),김미자(Mi-Ja Kim),남궁승(Seung Namkoong),구현정(Hyun-Jung Koo),손은화(Eun-Hwa Sohn) 한국산학기술학회 2021 한국산학기술학회논문지 Vol.22 No.9

본 연구에서는 담배필터를 통해 호흡기로 노출되는 주류연 흡연 장치를 구현하여 직접 흡연에 의한 주류연의 노출이 면역시스템에 미치는 영향을 생쥐모델을 이용하여 규명하고자 하였다. 실험동물(n = 15)은 대조군(sham군)과 담배 2개비/일 노출군 및 4개비/일 노출군으로 각각 나누고, 7일 동안 주류연을 노출시킨 후 체중변화와 면역기관 지수를 측정하였다. 주류연 노출이 면역세포의 기능에 미치는 영향을 확인하기 위해, lipopolysaccharide(LPS) 또는 concanavalinA(ConA) 자극에 대한 비장세포와 흉선세포의 증식력과 nitric oxide(NO) 분비량을 측정하였다. 실험결과, 4개비/일 고용량 노출군의 흉선, 비장, 간 무게가 대조군에 비해 각각 0.3, 0.2 및 0.2배 유의적으로 감소하였고(P<0.05), 흉선세포 생존율과 B세포 및 T세포 증식력이 감소하였다. 또한, LPS에 의한 비장세포의 NO 분비능도 감소하였다. 본 연구는 주류연 노출이 흉선과 비장에 직접적인 손상을 주며, 자극원에 대한 면역세포의 증식력과 NO 분비 등 방어능력을 유의적으로 약화시킨다는 것을 보여주었다. 이는 흡연과 관련된 면역질환 발생이 주류연의 노출에 대한 직접적인 면역기관의 손상과 자극에 대한 방어 기능의 약화가 관련되어 있음을 제시한다. This study investigated the effect of exposure to mainstream smoke (MS) through cigarette filters on the immune system in mice. Fifteen mice were divided into 3 groups; control (sham), 2 cigarettes smoked/day (CS), 4 CS. Changes in body weight and organ index were measured after exposure to MS for 7 days. The effects of exposure to MS on nitric oxide (NO) production and cell proliferation induced by lipopolysaccharide (LPS) or concanavalin A (ConA) in murine lymphocytes were also determined. Exposure to 4 CS reduced the organ indices of the thymus, spleen, and liver by 0.3-, 0.2-, and 0.2-fold (P<0.05), respectively. Exposure to MS also inhibited the lymphocyte proliferation by LPS or ConA and the NO production by LPS. This study confirmed that the immune organs were directly damaged as well as weakened in their cell function by exposure to MS. It provides direct evidence that the occurrence of various immune diseases caused by smoking is closely related to the impairment of immune functions by exposure to MS.

백혈병 청소년의 회복력 영향 요인

홍성실(Hong, Sung Sil),박호란(Park, Ho Ran) 한국간호과학회 2015 Journal of Korean Academy of Nursing Vol.45 No.4

Purpose: The purpose of this study was to identify the factors relating to resilience for adolescents with leukemia and examine the relationship between these factors. Methods: From June to September in 2014, 199 adolescents aged 11 to 21 participated in the study as they visited the out-patient clinic at C university hospital for follow-up care. To verify the predictors and the effects of resilience, uncertainty, symptom distress, perceived social support, spiritual perspective, defensive coping, courageous coping, hope, and self-transcendence were measured. Collected data were analyzed using hierarchical regression analysis with the SAS statistics program. Results: The final regression model showed that courageous coping, hope, and self-transcendence were significant predictors related to resilience in adolescents with leukemia and explained for 63% of the variance in resilience. Conclusion: The findings indicate that adolescent-oriented intervention programs enhancing courageous coping, hope, and self-transcendence should be provide for adolescents with leukemia in order to overcome illness-related stress and support physical, psychological and social adjustment.

백혈병 생존 청소년의 신체적ㆍ정서적 후유장애, 사회적응 및 삶의 질

홍성실(Hong, Sung Sil),박호란(Park, Ho Ran),김광성(Kim, Kwang Sung),최선희(Choi, Sun Hee) 한국간호과학회 2014 Journal of Korean Academy of Nursing Vol.44 No.1

Purpose: This study was conducted to examine the late effects, social adjustment, and quality of life in adolescents who had been completely treated for childhood leukemia and their parents. Methods: Participants consisted of 41 pairs of adolescent survivors (13-18 years) and their parents. Parents checked for their child"s physical late effects. The Korean Version of Post-Traumatic Symptoms for psychological late effects, social functioning questionnaire for social adjustment and the PedsQL 4.0 Generic Core Scales for quality of life were completed by adolescents and parents. Data were analyzed using SPSS. Results: Twenty out of 41 adolescents had one or more physical late effects. Adolescents showed more serious psychological late effect than parents. Five children and seven parents had above cut-off scores and they were considered the high risk group for posttraumatic symptoms. Parent-reported scores were significantly higher than child-reported scores in terms of social adjustment and emotional functioning of quality of life. Low school functioning in adolescents was associated with physical late effects. Conclusion: The results indicate that long-term and systematic management for childhood leukemia survivors affect positive social adjustment and can further improve quality of life.

간호학생의 영성과 임상실습 스트레스

홍성실(Sung-Sil Hong),홍정아(Jung-Ah Hong),전원희(Won-Hee Jun) 한국콘텐츠학회 2013 한국콘텐츠학회논문지 Vol.13 No.6

본 연구는 간호학생의 영성과 임상실습 스트레스 정도를 평가하고 이들 간의 관계를 파악하여 간호학생들의 영성증진과 임상실습 스트레스 중재방안으로 영성의 활용가능성을 확인하고자 시도하였다. 2개 대학의 간호학생 203명을 편의 추출하여 자가 보고식 설문지를 사용하여 자료를 수집하였다. 연구결과, 본 연구 대상자들의 영성은 5점 만점에 3.18점, 임상실습스트레스는 5점 만점에 3.42점으로 중간이상의 수준이었다. 하위영역별로는 영성의 경우 ‘삶의 의미와 목적’이 가장 높았고, 임상실습 스트레스에서는 ‘실습교육환경’에 대한 스트레스가 가장 높게 나타났다. 일반적 특성에 따른 차이에서는 영성은 종교와 전공만족도에 따라, 임상실습 스트레스는 성별과 전공만족도에 따라 유의한 차이를 보였다. 영성과 임상실습 스트레스는 유의한 부적상관관계가 있었다. 결론적으로, 간호학생들의 임상실습 스트레스를 감소시키기 위해서는 영성증진을 위한 교육프로그램 개발과 이를 효과적으로 교육과정에 접목시킬 수 있는 방안을 구축할 필요가 있다. The purpose of this study was to investigate the level of spirituality and stress of clinical practice and to identify the relationship of variables among nursing students. Data were collected from 203 nursing students using self-report questionnaire composed of demographic characteristics and two scales including spirituality and stress of clinical practice. Data were analyzed by frequencies, t-test, ANOVA, Pearson‘s correlation coefficients using the SPSS program. The mean score for spirituality and stress of clinical practice were above medium level, with the value of 3.18 and 3.42 out of 5 respectively. There were significant mean differences in spirituality according to religion and satisfaction on major while those in stress of clinical practice according to sex and satisfaction on major. Significant negative correlation between spirituality and stress of clinical practice was found. In conclusion, to reduce clinical stress in nursing students, it is necessary to develop and apply educational program for spiritual improvement.

노인간호 교육이 간호대학생의 노인에 대한 지식과 노인간호 태도에 미치는 효과

홍성실(Hong, Sung Sil),조은주(Jo, Eun Joo),박정숙(Park, Jung Suk) 고신대학교 전인간호과학연구소 2015 전인간호과학학술지 Vol.7 No.-

Purpose: The purpose of this study was to examine the effects of gerontological nursing education on knowledge and attitude for the aged. The participants were 37 in third-year nursing students at K university in Busan, Korea. Method: The data was collected from Aug. 27th toward Nov. 26th, 2012. Result: As a result, the knowledge of nursing university students has been improved after the gerontological nursing education , the knowledge score of physical, physiological and social realm. There has been no significant change by the gerontological nursing education in the attitude of nursing university students for the Aged. Conclusion: Through this the gerontological nursing education in the future may be thought to change the knowledge and attitude of nursing university students affirmatively if the education program grafting the theoretical education together with the practice education is applied by apprehending the level of understanding and demand for the gerontological nursing of students

대학생의 특성분노 수준에 따른 특성분노 영향요인

전원희,홍성실,Jun, Won Hee,Hong, Sungsil 경희대학교 동서간호학연구소 2017 동서간호학연구지 Vol.23 No.2

Purpose: The purpose of this study was to investigate factors affecting trait anger level in undergraduate students. Methods: The participants consisted of 315 undergraduate students at the universities in the P and D cities. Data were collected with a structured questionnaire and analyzed using descriptive analysis, t-test, ANOVA, Pearson correlation coefficients, and multiple stepwise regression with the SPSS/Win 20.0 program. Results: The low trait anger group showed lower levels of anger-out, anger-in and depression compared to the high trait anger group. The anger-control and positive thinking were higher in the low trait anger group than that of high trait anger group. The result of multiple stepwise regression showed that both depression and anger-out were predictors in trait anger in the both groups. In the low trait anger group, anger-control, anger-in, depression, and anger-out were significant factors of trait anger, which accounted for 31% of the total variance. High trait anger level was affected by depression, anger-out and positive thinking, which explained 40% of trait anger. Conclusion: The results of this study suggest that anger management program for undergraduate students should consider trait anger level as well as approaches with customized strategies according to trait anger level.

AHP분석을 통한 도시재생사업 평가지표 연구

이정동(Lee, Jeong Dong),김영(Kim, Yeong),홍성실(Hong, Seong Sil) 한국주거환경학회 2017 주거환경(한국주거환경학회논문집) Vol.15 No.3

The approach in the policies of local development is on the increase for the revitalization of existing old downs which have undergone rapid decline due to the regional problems such as the development of new towns, suburban development, and the reorganization of industrial structure, which are newly emerging. While environmental urban generation projects such as residential environment redevelopment projects and the Urban Renewal Promotion Law have been conducted so far, new types of sustainable urban regeneration are undertaken in various ways under the Urban Regeneration and Assistance Act. Many municipalities across the country are making efforts to achieve the revitalization of old downtown due to the decline in construction business caused by economic recession, the collapse of community in existing cities, and low resettlement rate of native residents. In addition, individual local governments should establish and implement strategic urban planning for regeneration and urban revitalization by selecting urban revitalization regions under the recent urban regeneration Special Act. Investigating main contents for the establishment of strategic planning for regeneration, the fundamental thing is to establish the revitalization plans of urban regeneration targeting the regions of urban regeneration and revitalization.

추출용매에 따른 오레가노 종자 분획물의 항산화 및 유지산화안정성 평가

이현종(Hyun-Jong Lee),김민아(Min-Ah Kim),홍성실(Sungsil Hong),김미자(Mi-Ja Kim) 한국식품과학회 2021 한국식품과학회지 Vol.53 No.1

본 연구는 항산화 활성이 우수한 것으로 알려진 오레가노 종자를 80% 에탄올, 헥세인, 에틸 아세테이트, 부탄올, 증류수로 분획하여 시험관 내 항산화 활성을 평가하고 180oC로 산화된 옥수수 기름에 적용하여 유지 산화에 미치는 영향을 확인하였다. DPPH 라디칼 소거 활성은 오레가노 종자 에틸 아세테이트 분획물의 라디칼 소거 활성이 92.3%으로 분획물 중 가장 높게 나타났으며, ABTS 양이온 라디칼 소거 활성 측정 결과 오레가노 종자 에틸 아세테이트 분획물의 ABTS 양이온 라디칼 소거 활성이 53.9%로 분획물 중 가장 높게 나타났다. FRAP 환원력은 오레가노 종자 에틸 아세테이트 분획물이 6,130 μmol ascorbic acid equivalent/g extract로 분획물 중 가장 높게 나타났으며, 총 페놀함량은 FRAP 환원력의 결과와 마찬가지로 오레가노 종자 에틸아세테이트 분획물이 770 μmol tannic acid equivalent/g extract로 분획물 중 가장 높은 결과를 나타내었다. 오레가노 종자 분획물간 헤드스페이스 산소 함량은 80분 이상 산화가 진행되면서 오레가노 종자 헥세인, 에틸 아세테이트, 80% 에탄올 분획물을 첨가한 시료군이 대조군에 비해 각각 1.25, 1.05, 1.02배 많은 산소함량 차이를 보였다. 유지의 일차 및 이차 산화생성물을 평가한 CDA, ρ-AV의 실험결과 CDA가는 오레가노 종자 에틸 아세테이트 분획물을 첨가하였을 때 대조군에 비해 conjugated dienes 생성이 1.5배 유의적으로 감소한 것을 관찰하였다. ρ-AV의 결과 또한 CDA가와 마찬가지로 오레가노 종자 에틸 아세테이트 분획물을 첨가한 유지 시료의 이차 산화물 양이 대조군에 비해 1.26배 유의적으로 감소된 것이 관찰되었다. 이와 더불어 오레가노 종자에틸 아세테이트 분획물의 GC 분석 결과 주요 휘발성 성분은 카바크롤, 타이모퀴논 등으로 나타났다. 이러한 오레가노 종자의 항산화 활성은 에틸 아세테이트를 사용한 분획물에서 크게 나타났으며, 이는 오레가노 종자가 가지고 있는 향기 성분 속 항산화물질이 실험에서 사용한 다른 용매들 보다 에틸 아세테이트 속에 다량 용출되어 높은 항산화 활성을 나타낸 것으로 사료되며, 오레가노 종자 에틸 아세테이트 분획물 속에 포함된 테르펜 및 폴리페놀 등의 물질로부터 기인하는 것으로 생각된다. 따라서 본 연구 결과, 향후 오레가노 종자를 천연항산화제로 활용하기 위하여 에틸 아세테이트 분획물의 활용이 유용할 것으로 판단된다. The in vitro antioxidant activity of oregano seed fractions, fractionizing with 80% ethanol, n-hexane, ethyl acetate, n-butanol, and water, was evaluated, and their effects on edible oils were determined in corn oil at 180℃. The ethyl acetate fraction had the highest radical-scavenging activity. The ferric reducing antioxidant power activity and total phenol content of the ethyl acetate fraction were determined as 6,130 μmol ascorbic acid equivalents/g extract and 770 μmol tannic acid equivalents/g extract, respectively, which were significantly higher than those of the other fractions (p<0.05). Primary and secondary oxidation products in corn oil added with the ethyl acetate fraction of oregano seed significantly decreased by 1.5 and 1.26 times, respectively, compared with those in the control groups. The major volatile ingredients in the ethyl acetate fraction of oregano seeds were determined to be carvacrol, thymoquinone, and 3- cyclopentylcyl-cyclopentan-1-one. Ethyl acetate is a suitable solvent for extracting antioxidant compounds from oregano seeds and can be used as a natural antioxidant.