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RISS 인기검색어
- 검색키워드
- 저자 : 한경환 ( Kyung Hwan Han ) (검색결과 3 건)
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Isolation and Culture of Mesophyll Protoplasts from in vitro Cultured Populus alba × P . glandulosa
박용구,한경환 ( Young Goo Park,Kyung Hwan Han ) 한국산림과학회 1986 한국산림과학회지 Vol.73 No.1
N/A This study was carried out to investigate the optimum conditions for isolation and culture of mesophyll protoplasts from Populus alba × P. glandulosa. The results obtained from the experiments are as follows; 1) The suitable concentration of BAP for shoot multiplication was 0.4 ㎎/ℓ. 2) High yield and viability of isolated protoplasts were obtained by our high enzyme-short time incubation method. 3) Optimum enzyme concentrations for mesophyll protoplast isolation were Cellulase 2%, Macerozyme 0.8%, Hemicellulase 1.2%, Driselase 2%, and Pectolyase Y-23 0.05%. 4) 0.6M mannitol in enzyme solution was the most effective for protoplast isolation and viability. 5) The most adequate pH level of enzyme solution was pH 5.6. 6) The effect of DTT and MES buffer was significant. 7) For protoplast purification, 0.6M sucrose was the most proper concentration. 8) The adding effect of Dextran T40 in floating solution was important. 9) The mesophyll protoplasts isolated through our high enzyme-short time incubation method revealed successful response to culture condition over 3 weeks of culture.
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박원제 ( Won Jei Park ),한경환 ( Kyung Hwan Han ),권순효 ( Soon Hyo Kwon ),박미옥 ( Mi Ok Park ),구본학 ( Bon Hak Koo ) 한국환경복원기술학회(구 한국환경복원녹화기술학회) 2012 한국환경복원기술학회지 Vol.15 No.2
This study is carried out to find the most optimal soil- plant combination in the urban agriculture by analyzing the association of soil base material which is being used in the urban agriculture with the growth of plants. 4 types of easily purchased soil (bed soil(A), animal vermicast soil(B), earth worm soil(C) and matured compost(D)) verified in aspects of effect and safety of soil in terms of growth of crop is selected as experimental soil and B, C, D type soils are mixed with granite soil at the ratio of 7:3. And granite soil (E) is set as a controlled soil and is compared to verify the effect of the experimental mixed soil. Herbaceous plants are classified into the fruit vegetables (Lycopersicon esculentum Mill, and Capsicum annuum L.), leafy vegetables (Brassica campestris L, ssp, Pekinensis and Lactuca sativa L.), medicinal vegetables (Chrysanthemum zawadskii var, latilobum and Liriope platyphylla F, T, Wang & T, Tang), The results of comparison of growth of herbaceous plants in different soil types showed that fruit vegetables and leafy vegetables in general had excellent growth in D type soil mixture and Atypesoil in general and had the poorest growth in E type (controlled) soil. ``Chrysanthemum zawadskii var. latilobum`` had the excellent growth in D type, B type, C type mixed soil and A type soil and E type (controlled) soil are followed in order. In the case of ``Liriope platyphylla F. T. Wang & T. Tang``, the difference in growth by each soil was shown to be insignificant. Therefore, the soil applied in the urban agriculture varies depending on each species of herbaceous plants, but it is considered effective to cultivate herbaceous plant which is economical and productive by using D type mixed soil which can be recycled and inexpensive compared with other experimental soils in the urban agriculture.
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