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Evaluation of sediments of the waste from beer fermentation broth for bioethanol production
하정환,박중곤,Min-Kyung Gang,Taous Khan 한국화학공학회 2012 Korean Journal of Chemical Engineering Vol.29 No.9
As our previous studies showed, the waste from beer fermentation broth (WBFB) is a potential resource for bio-ethanol production. The original WBFB was superior to the supernatant in this regard. The current study investigates the potential of the WBFB sediment alone for bio-ethanol production after it has been diluted with distilled water or a chemically-defined medium. The effect of stock time on WBFB sediments for ethanol production was also studied. The fermentations were carried out using 50 ml vials placed in a bioreactor in static conditions. There was relatively little increase in ethanol production with fermentation time (up to 2 h) and stock time (up to 7 days) using 20% (v/v)sediment in distilled water which did not contain any nutrients or enzymes. A 2.09% increase in ethanol production was recorded after 2 h fermentation with 20% (v/v) WBFB sediments (1 day old) in a chemically-defined medium. The increase was 3.25% for WBFB sediments with a stock time of three days in a chemically-defined medium. The results also showed some residual activity of starch hydrolyzing enzymes in the sediments, especially at 60 oC. The overall results of this study revealed that the sediments alone were capable of bio-ethanol production even though they were five-fold diluted with distilled water or the chemically-defined medium.
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하정환,박중곤 한국화학공학회 2012 Korean Journal of Chemical Engineering Vol.29 No.5
A single sugar α-linked glucuronic acid based oligosaccharide (SSGO) is water soluble oligosaccharides (WSOS) obtained by Gluconacetobacter hansenii PJK (KCTC 10505BP) as a byproduct during bacterial cellulose (BC) production. In this study, SSGO was used for the improvement of BC production by the vinegar bacterium, Acetobacter xylinum, which produces heteropolysaccharides as a byproduct. The addition of 1.0% SSGO to the chemically defined medium (CDM) resulted in an 89.3% increase in BC production by A. xylinum after 15 days of cultivation under static condition, and a 52.3% increase in BC production by G. hansenii. Both the dry cell weight and live cell density of A. xylinum increased 50% with the addition of 1.0% SSGO. SSGO successfully improved BC production by A. xylinum.
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肝吸蟲感染 白鼠 에 있어서 Praziquantel(Embay 8440)의 殺蟲效果에 관한 實驗的 硏究
河正煥 고려대학교 의과대학 1980 고려대 의대 잡지 Vol.17 No.3
Experimentally infected ats with about 50 metacercariae of Clonorchis Sinensis in each were treasted orally with different dosages of praziquantel. About 100 rats were divided into 8 groups in each developmental weeks after infection(from the first to 8th week after infection) and 24 rats served as untreated controls. In each developmental weeks after infection, about 15 rats divided into 4 groups including control in each 3 to 5 rats were used to determine for the curative activity of praziquantel against C. sinensis. The experimentally infected rats in each developmental weeks after infection received praziquantel 1×100㎎/㎏×3 days, 3×100㎎/㎏×1 days and 3×100㎎/㎏×2 days. The effects of the drug were evaluated by the worm recovery rates, morphological changes of the worms and number of eggs per gram of feces(EPG) after treatment. The feces of individual rats were collected once in a week at one week before and 4 weeks after treatment, and the number of Clonorchis eggs were examined by Stoll's egg counting method. The necropsy was performed on 4 weeks after treatment. The liver and the bile ducts were examined immediately after the dissection, and all of the worms recovered from the liver immersed in warm saline solution in a small petri dish in order to observe the motility of indicidual worms. After that, all worms obtained from the treated and untreated control rats were fixed in 10% formalin solution. The whole mount specimens were prepared by Semichon's acetocarmine stain for the study of morphological changes. In the treatment with dosage of 1×100㎎/㎏ for 3 consecutive days, relatively low worm recovery rates were shown in the early stages of infection than those of control groups. However there was no practically big differences of the worm recovery rates between the later stages of infection(7 to 8 weeks after infection) and control groups. In the early stages of infection, the worms recovered in the bile ducts of each treated rats showed morphologically intact as a normal control groups, however in the later stages of infection the worms showed already damaged their reproductive organs such as testes, seminal receptacle and ovary etc. In the treatment with dosage of 3×100㎎/㎏×1 days, the recovery rates of the worms in all developmental weeks after infection were considerable low and some growth inhibition of the worms were also noted. The worms recovered from the bile ducts in all developmental stages were seriously damaged in their reproductive organs, such as testes, seminal receptacle, ovary, Mehlis' gland, uterus and vitelline glands etc. The hyaline degenerations were also observed on the parenchymal tissues in the dead worms. The mean EPG values in this group were markedly reduced at the first 2 weeks after treatment. However the low values of EPG were generally noted at the 3rd to 4th week after treatment. On the other hand, in the treatment with dosage of 3×100㎎/㎏×2 days, no more the living worm was recovered in all developmental stages of Clonorchis. The dead worms recovered from the bile ducts were so seriously damaged that the morphological cahnges could not be observed. The mean EPG values in this group were markedly reduced during the first 2 weeks after treatment. At the 3rd week after treatment EPG value became negative in the most of all treated groups. No more the Clonorchis eggs were detected at the 4th week after infection, but only a very small numbers of eggs were found by the concentration method. From the above results, it is obvious that the present study shows that praziquantel exhibits a high degree of activity against Clonorchis sinensis in the rats, with no apparent differences in the efficacy against each developmental stages.
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Yoon`s Falope Ring에 의한 난관 불임수술시 Lidocaine Jelly의 효과
하정환(JH Ha),박영동(YD Park),김재욱(JU Kim),곽현모(HM Kwak) 대한산부인과학회 1976 Obstetrics & Gynecology Science Vol.19 No.4
1975년 2월19일부터 동년 4월23일까지 연세대학교 의과대학 산부인과교실에서 Yoon`s falope ring에 의한 복강경난관 결찰술을 한 162예를 선택하여 Lidocaine jelly를 좌측난관에 도말하여 다음과 같은 결론을 얻었다. 1. 대상자의 연령별 분포는 35-39세가 43.2%였고 생존자녀수는 3명이 41.4%로 수위를 차지 하였다. 2. 교육정도는 대부분이 중,고등학교 정도였다. 3. 수술소요시간은 98%에서 15분이내였다. 4. 대상자 162예중 147예 90.7%에서는 양측공히 동통이 없었으며 9.3%에서만이 동통을 호소 하였다. 동통을 호소한 9.3%(15예)중 lidocaine jelly를 도말한 좌측난관에 동통을 호소하지 않 앗던 경우가 6예, 반대로 동통을 호소한 예가 6예엿다. 본 연구에서 국소마취제 도말이 완전히 동통을 제거할 수는 없었으나 다소나마 동통을 감소 시킬수는 잇었으며 이것이 난관결찰술후의 동통에 대한 문제를 해소하는 충분한 방법은 못 되었다. A clinical study was carried out for the evaluation of the pain control effect of lidocaine topical anesthetic application on the tube by means of Yoon`s ring laparoscopic female sterilization at Yonsei University medical Center Total 162 cases of laparoscopic tubal sterilization by Yoon`s ring were performed by unilateral application of Lidocaine as out patient bases from February 19, 1975 to April 30, 1975. We were not able to evaluate the true effect of the local anesthetics, lidocaine, in the presence of preoperative sedation with Demerol and valium, but as a result some analgesic effect was noted in several cases. for more detailed effect of lidocaine ointment on laparoscopic sterilization further study was needed.
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나사룰라,하정환,박중곤 한국생물공학회 2010 Biotechnology and Bioprocess Engineering Vol.15 No.1
The effect of agar plates on the bacterial cellulose (BC) production in a static culture was investigated in order to find the role of agar component as a surface modifying agent. Two types of surface modified reactors (SMRs: SMRD and SMRB) were prepared by coating the bottom of the reactors with agar dissolved in distilled water and basal medium, respectively. The SMRs were used for BC and water soluble oligosaccharides (WSOS) production. Control was done by the same procedure using reactors without agar plate. In both types of SMRs, the maximum production rate was observed after the second day of cultivation compared to third day of cultivation in the case of the control. The maximum productions of BC 5.308 and 5.472 g/L were observed at the first batch using SMRs prepared with agar dissolved in distilled water (SMRDs) and SMRs prepared with agar dissolved in a basal medium (SMRBs), respectively. Similarly, in the daily-culture and successive batch strategy experiments the maximum amount of WSOS produced in the SMRs was almost double that of the control. The highest water holding capacity value 92.21 g/g was observed for BC formed in the SMRs prepared with 3.0% of agar. FTIR and XRD analyses were carried out to study the structural features of the prepared BC.
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