한국동물학회 제 5회연구발표회 기념 및 질의응답

최태주 한국통합생물학회 1961 동물학회지 Vol.4 No.2

Rhodopsin 의 再生에 對한 Nicotinamide 와 Folic Acid 의 影響에 關한 硏究

강성호,최태주,Kang, Seoung-Ho,Choi, Tae-Joo 대한화학회 1962 대한화학회지 Vol.6 No.1

Six frogs (Rana nigromaculata 25∼40 gm) were adapted to light for 2 hours. Then 0.02 ml of 3% nicotinamide and 0.02 ml of 0.6% folic acid were injected into the vitreous body of the right eye-ball, and 0.02 ml normal saline solution into the vitreous body of the left eye-ball respectively. After dark adaptation for an hour-their heads were cut off under a dim red light (650 $m{\mu}$). The retinae were removed from the left eye-ball for the control group and from the right for the test group respectively. Then rhodopsin was extracted from the retinae with 3 ml of 2% digitonin solution(pH = 7.0) for 17 hours at 0$^{\circ}C$ in the dark. The optical densities before and after the illumination of the extract were measured and compared with those of the control group. The results are as follows: 1) The group which had been injected with 0.02 ml of 3% nicotinamide solution had the promotive action on tile regeneration of rhodopsin in comparison with the control group. 2) The group which had been injected with 0.02 ml of 0.6% folic acid solution had the controlling action on the regeneration of rhodopsin in comparison with the control group.

Rhodopsin의 再生에 對한 Inositol과 Pantothenic Acid의 影響에 關한 硏究

姜成浩,崔泰周 이화여자대학교 한국문화연구원 1963 韓國文化硏究院 論叢 Vol.4 No.-

Five frogs (Rana nigromaculata 25-40 gm ) were adapted to light for 2 hours. Then 0.02 ml of 2 mol inositol and 0.02 ml of 3% Na-D-pantothenate solution were injected into the vitreous body of the left eye ball, and 0.02 ml normal saline solution into the vitreous body of the right eye ball respectively. After dark adaptation for an hour their were cutt off under a dim red light (>650 mμ). The retinae were removed, from the right eye ball for the control group and from the left for the test group respectively. Then rhodopsin was extracted from the retinae with 3 ml of 2% digitonin solution for 15 hours at 0℃ in the dark. The optical densities before and after the illumination of the extract were measured and compared with those of the control group. The results are as follows; 1) The group which had been injected with 0.02 ml of 2 mol inositol solution had the controlling action on the regeneration of rhodopsin in comparison with the control group. 2) The group which had been injected with 0.02 ml of 3% Na-D-pantothenate solution had the promotive action on the regeneration of rhodopsin in comparision with the control group.

Rhodopsin의 再生에 대한 Amino acid의 影響에 關한 硏究[第二報] : glutathione과 tryptophan의 영향

姜成浩,崔泰周 이화여자대학교 한국문화연구원 1966 韓國文化硏究院 論叢 Vol.8 No.-

Five frogs (Rana nigromaculata, body weight: 25~40g) were adapted to light for two hours. Then 0.02ml. of 3% glutathione and 0.02ml. of 0.1% tryptophan solution were injected the vitreous body of right eye ball, and 0.02ml. of normal saline solution into the vitreous body of the left eye ball respectively. After dark adaptation for an hour their heads were cut off under the dim red light (>650mμ). The retinae were removed, from the left eye ball for the control group and from the right for the test group respectively. Then rhodopsin was extracted from the retinae with 3ml. of 2% digitonin solution for 17 hours at 0℃ in the dark. The optical densities before and after the illumination of the extract were measured and compared with those of the control group. The results are as follows; 1) The group which had been injected with 0.02ml. of 3% glutathione solution had the promotive action on the regeneration of rhodopsin in comparison with the control group. 2) The group which had been injected with 0.02ml. of 0.1% tryptophan solution had the promotive action on the regeneration of rhodopsin in comparison with the control group.

한국 고유주의 일종인 막걸리에 대한 미생물학적 연구

고춘명,최태주,류준,Koh, Choon-Myung,Choi, Tae-Joo,Lew, Joon 한국미생물학회 1973 미생물학회지 Vol.11 No.4

This study included two parts of investigation, the microfloral changes during the brewing process with the changes of pH, total acidity, temperature and alcoholic contents, as well as determination of survival times of major enteric pathogens in Takju. 1. Maximum number of Saccharomyces cerevisiae was $4.3{\times}10^7$ per milliliter on the 5th day of fermentation and gradually decreased. Saccharomyces cerevisiae was one of the predominant strains of the fermentation process. The number of Saccharomyces cerevisiae was $4.3{\times}10^6$ per milliliter at the completion of the brewing and human consumption. In a few days after the completion of the brewing. Bacillus subtilis and some species of Staphylococcus spp. began to grow and those organisms were responsible for the spoilage. 2. Maximum pH, during the brewing, was 5.8 on the first day of fermentation and rapidly decreased until 6th day of fermentation at pH 4.3. 3. Maximum alcholic content was 14.5 degree on the 4th day of fermentation, 10.3 degree on the 5th day and this degree was continued during the experimentation. 4. Maximum temperature, during Takju brewing was 34.deg.C on the 3rd day of fermentation and rapidly decreased up to 23.deg.C on the 6th day and this temperature was continued until the brewing process was finished. 5. Maximum total acidity was 0.57 percent on the 4th day of fermentation and gradually decreased by brewing process was completed. 6. Survival time of major enteric pathogenic bacteria in Takju was as follows : Shigella dysenteriae and Escherichia coli were isolated in two hours and 14 hours respectively, but Salmonella typhi, Vibrio parahemolyticus were not isolated even in an hour after the inoculation of those organisms in undiluted Takju. In diluted Takju, Salmonella typhi, Vibrio parahemolyticus were not isolated even in an hour after the inoculation of those organisms in undiluted Takju. In diluted Takju, Salmonella typhi, Shigella dysenteriae, and Escherichia coli were survived for 50-60 hours, but Vibrio cholerae and Vibrio parahemolyticus were not isolated even if treated within one hour.

抗生劑( Siccanin 및 Azalomycin-F )處理에 依한 Candida albicans의 電子顯微鏡的 觀察 : Siccanin and Azalomycin-F

高春明,崔泰周,등永健 최신의학사 1973 最新醫學 Vol.16 No.6

The present study is an ultrafine structure of Candida albicans treated with Siccanin and Azalomycin F by means of electron microscopy. The results as follows: 1. In contrast to the bacteria, the normal Candida albicans contains nuclear envelop, mitochondria, endoplasmic reticulum, distinct cell wall, cell membrane and nucleus as observed in the eucaryotic cells. 2. Treated Candida albicans with Siccanin and Azalomycin-F was changed their morphology and cytoplasmic components, for examples, thicking of cell wall, partial delation of nuclear envelop, mictochondria, detachment of cell membrane from cytoplasm and increasing of lipid particles. 3. When the cells were treated with the tested antibiotics at 10 mcg per ml. for 60 minutes, the cellular damges were most severe.

각종 식품중의 유독성 진균에 관한 연구 5

고춘명,최태주,류준,Koh, Choon-Myung,Choi, Tae-Joo,Lew, Joon 한국미생물학회 1973 미생물학회지 Vol.11 No.2

Thirtythree strains of the Aspergillus spp. isolated from foodstuffs were observed through some physiological characteristics for detection of identification key of Aspergillus spp. 1) Each strain of Aspergillus spp. had their specific characteristics and could be used for identification of species. 2) Excellent amylase-producing fungi were observed among the isolated strains of Aspergillus spp. 3) Amylase activities increased for one week incubation period. 4) In the tests of common characters of aflatoxin-producing fungi among the 33 strains of Aspergillus spp., for example, conidial size, presence of sclerotia, kojic acid, and pigment production, coloration of phenol, reduction of methylene blue, etc.

Mycotoxins에 관하여

고춘명,최태주,류준,Koh, Choon-Myung,Choi, Tae-Joo,Lew, Joon 한국미생물학회 1972 미생물학회지 Vol.10 No.3

한국 토괴균의 계속적인 연구로서, 분리된 균류중 Aspergillus 8종과 microfungi 5종인 Alternaria alternata, Chrysosporium pannorum, Doratomyces microsporus, Trichoderma koningii, T. viride에 관하여 보고하였다. 이들 동정된 균류 중 아직까지 한국에서 미기록된 것으로는 Chrysosporium pannorum, Doratomyces microsporus, Trichoderma koningii, T. viride 등의 4종이었다. 또한 완전연대인 Emericella nidulans var. nidulans에 관하여 보고하였다. Fourteen species of the hyphomyceteous fungi isolated from Korean soils are described and illustrated. Among these, one species has teleomorphic state and is identified as Emericella nidulans var. nidulans, similar to Emericella spectabilis with the exception of size of the conidiophores as well as color and the arrangement of the hulle cells. Four species of hyphomyceteous fungi. Chrysosporium pannorum, Doratomyces microsporus, Trichoderma Roningii, T. viride, are reported here for the first time in Korea.

急性一酸化炭素中毒의 解毒作用에 關한 硏究

姜成浩,崔泰周,任舜美 이화여자대학교 한국문화연구원 1963 韓國文化硏究院 論叢 Vol.4 No.-

The effects of ascorbic acid upon carbon monoxide poisoning in dogs and guinea pigs are summarized as follows: 1) The carbonyl hemoglobin level in the circulating blood of carbon monoxide poisoned dogs was rapidly decreased when ascorbic acid was administered orally. Thirty minutes after administration of ascorbic acid, 60% of total carbonyl hemoglobin was decreased in the test group, while only about 14% was decreased in the control group. 2) After carbon monoxide poisoning the value of hemoglobin and the number of erythrocytes and leukocytes were increased. However, their return to normal values was promoted by administration of ascorbic acid. 3) The amount of plasma ascorbic acid in dog was increased by carbon monoxide poisoning. 4) Ascorbic acid in various organs of carbon monoxide poisoned guinea pigs were estimated. The amount of ascorbic acid and dehydroascorbic acid in the cerebrum, muscle, and blood plasma was decreased, whereas in the kidney, adrenal gland, liver, and spleen there was no change. 5) Ascorbic acid in various organs of guinea pigs was estimated when they recovered to walk from poisoning. Ascorbic acid in the kidney and cerebellum was apparently decreased compared with the value estimated immediately after poisoning. In the adrenal gland and spleen ascorbic acid(reduced from) was increased and dehydro form was decreased. 6) Clinical symptoms, e.g. falling down and getting up of carbon monoxide poisoned dogs and guinea pigs seemed to have no direct relations with carbonyl hemoglobin level in blood.

Coccidioides immitis의 mycelial phase와 tissue phase에 있어서의 전자현미경적 관찰

고춘명,최태주,등영건,류준,Koh, Choon, Myung,Choi, Tae-Joo,Deung, Young-Kun,Lew, Joon 한국미생물학회 1971 미생물학회지 Vol.9 No.4

Ultrastructural observations of mycelial and tissue phase with dimorphic fungal pathogen Coccidioides immitis were studied by electron microscopy of thin sections. 1. In mycelial phase of C.immitis contains normal cell components such as nucleus, mitochondria, endoplamsic reticulum, intracytoplasmic membrane system, cell wall and cell membrane as observed in the other encaryotic cells. 2. In tissue phase of C. immitis was larger than mycelial phase in cell size and observed much more vacuoles than mycelial phase. 3. In the contrast of mycelial phase of C. immitis, the tissue phase of cells were observed fibril form of capsular layer.