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최진남(Jin Nam Choi),송종태(Jong Tae Song),송상익(Sang lk Song),안지훈(Ji Hoon Ahn),최양도(Yang Do Choi),이종섭(Jong Seob Lee) 한국응용생명화학회 1995 Applied Biological Chemistry (Appl Biol Chem) Vol.38 No.1
To understand the molecular structure and pathogenesis mechanism of Korean garlic viruses, we have isolated cDNA clones for garlic viruses. The partial nucleotide sequences of 24 cDNA clones were determined and those of five clones containing poly(A) tail were compared with sequences of other plant viruses. One of these clones, V9, has a primary structure similar to the carlavirus group, suggesting that the clone V9 derived from a part of garlic latent virus (GLV). Northern blot analysis with the clone V9 as a probe demonstrated that GLV genome is 8.5 knt long and has a poly(A) tail. The clone V9 encodes coat protein (CP) of 33 kDa and nucleic acid binding protein of 10 kDa in different reading frame. The hexanucleotide motif, 5`-ACCUAA, which is conserved in the 3` noncoding region arid was proposed to be a cis-acting element involved in the production of negative strand genomic RNA was noticed. Complementary sequence to the hexanucleotide motif, 5`-TTAGGT, is also found in the positive strand of V9 RNA. The putative CP gene was cloned into the pRSET-A expression vector and expressed in E. coli BL21. The expressed recombinant V9CP protein was purified by Ni^(2+)·NTA affinity chromatography. The anti-V9CP antibody recognizes 34 kDa polypeptide which could be CP of GLV in infected garlic leaf extract. Immunoblot and Northern blot analysis of various cultivars shows wide occurrence of GLV in Korean garlic plants.
최진남(Jin Nam Choi),안지훈(Ji Hoon Ahn),최양도(Yang Do Choi),이종섭(Jong Seob Lee) 한국응용생명화학회 1993 Applied Biological Chemistry (Appl Biol Chem) Vol.36 No.4
To understand the molecular structure and pathogenesis mechanism of Korean garlic viruses (GV), virus particles were isolated from field-grown garlic leaves and RNA genome was isolated from them. It was used for constructing cDNA library for GV. Several cDNA clones for GV were isolated and classified into 4 different groups on the basis of cross Southern hybridization. Northern blot analysis of GV RNA with one of these cDNA clones shows that the clone is a cDNA for GV RNA.
Bacillus sp . E1 의 cyclodextrin 생산효소 유전자 분리 및 구명
용정식,최진남,박성순,박천석,박관화,최양도 ( Jeongsik Yong,Jin Nam Choi,Sung Soon Park,Cheon Seok Park,Kwan Hwa Park,Yang Do Choi ) 한국응용생명화학회 1997 Applied Biological Chemistry (Appl Biol Chem) Vol.40 No.6
To isolate a gene for cyclodextrin glycosyltransferase (CGTase) from alkalophilic Bacillus sp. El, polymerase chain reaction (PCR) amplification was carried out. Direct molecular cloning of 1.2 kbp fragment and partial nucleotide sequence analysis of the PCR amplified clone, pH12, showed close homology with CGTases from Bacillus species. To investigate the genomic structure of the gene, Southern blot analysis of genomic DNA was carried out with the clone pH12 as a molecular probe. It showed that 5.3 kbp XbaI fragment was hybridized with the probe pH12. To isolate a genomic clone, genomic DNA library was constructed and a genomic clone for CGTase, pCGTEl, was isolated. Nucleotide sequence analysis of the clone pCGTEl revealed that BCGTEl contained 2,109 bp open reading frame encoding a polypeptide of 703 amino acids and showed over 94.3% amino acid sequence homology with CGTase of β-cyclodextrin producer, Bacillus sp. KC201.
한국어-프랑스어 이중언어 아동의 동사 표현 양상과 다양성 -동작 명명하기 과제를 중심으로-
황순희 ( Soon Hee Hwang ),최진남 ( Jin Nam Choi ) 이중언어학회 2015 이중언어학 Vol.58 No.-
In this research, a total of 34 children (48 to 60 months old) belonging to 2 groups (Korean monolinguals, Korean-French bilinguals) were compared and their responses were analyzed during an action naming task based on video-clips, called APPROX to explore the verbal lexicon acquisition and their expressive diversity. Two groups differed significantly in rates of used lexicon, valid responses, referent verbs, error patterns and valid verbs` uses. Especially, on the expressive diversity, monolingual group exceeded bilingual; on the responses` validity, the best performance was achieved by bilingual children whose language of instruction differed from the language of testing. Thus, executive control outcomes for bilingual children are general but performance on verbal task is specific to factors in the bilingual experience.(Pusan National University & Universite de Toulouse II)
한국 마늘 Potexvirus 의 cDNA 유전자 분리 및 분포에 관한 연구
송종태(Jong Tae Song),최진남(Jin Nam Choi),송상익(Sang lk Song),이종섭(Jong Seob Lee),최양도(Yang Do Choi) 한국응용생명화학회 1995 Applied Biological Chemistry (Appl Biol Chem) Vol.38 No.1
To understand the molecular structure of Korean garlic viruses, cDNA cloning of virus genomic RNA was attempted. Virus particles were isolated from virus-infected garlic leaves and a cDNA library was constructed from garlic virus RNA. One of these clones, S81, selected by random sequencing has been identified as a member of potexvirus group other than potyvirus and carlavirus. The clone is 873 by long contains most of the coat protein (CP) coding region and 3`-noncoding region including poly(A) tail. A putative polyadenylation signal sequence (AAUAAA) and the hexanucleotide motif (ACUUAA), a replicational cis-acting element conserved in the 3`-noncoding region of potexvirus RNAs are noticed. The clone S81 shows about 30-44% identity in both nucleotide and amino acid sequences with CPs of potexvirusea The genome sine of the virus was analysed to be 7.46 knt by Northern blot analysis, which was longer than those of other potexviruses. The open reading frame encoding CP was expressed as a fusion protein (S81CP) in Escherichia coli and the recombinant protein was purified by immobilized metal binding affinity chromatography. Polyclonal antibody was raised against S81CP in rabbit to examine the occurrence of garlic potexvirus in Korean garlic plants by immunoblot analysis. Two virus protein bands of Mr 27,000 and 29,000 from garlic leaf extract of various cultivars reacted with the antibody. It was shown that Mr 27,000 band might not be a degradation product of Mr 29,000 band, suggesting that two types of potexvirus different in size of coat protein could exist in Korean garlic plants.
Intrinsic Motivation vs. Extrinsic Motivation: From Idea Generation to Implementation
정현선(Hyun Sun Chung),최진남(Jin Nam Choi),Thomas Sy 한국인사조직학회 2020 한국인사ㆍ조직학회 발표논문집 Vol.2020 No.3
To sustain organizational competitiveness, organizations must recognize the central importance of innovation and its implementation. Because teams are essential sources of innovation, this paper explores how intrinsic and extrinsic motivations influence affective and normative organizational commitments when teams engage in bottom-up innovation that leads to actual implementation. Given the growing recognition of the importance of team-level innovation processes, this study also delves into how various types of motivation can advance team-level innovation processes from idea generation to implementation. A sample of 60 teams from various organizations indicates that when teams utilize extrinsically driven idea generation, they form affective and normative commitments to implementing innovations. However, when teams utilize intrinsically driven idea generation, they establish normative commitments for innovation implementation. The empirical findings indicate that extrinsically driven idea generation may have a stronger influence on establishing commitments to an idea, thereby leading to its implementation. By showing that team-level motivation to implement innovations may function differently from individual-level motivation, this paper explains how to manage teams’ motivation to implement effective innovations.