대청에서 AFLP를 이용한 종자순도검사와 평가

최주수,허만규,성정숙 한국약용작물학회 2009 한국약용작물학회지 Vol.17 No.3

Isatis tinctoria var. yezoensis (Ohwi) Ohwi (Cruciferae) is one of major natural dyeing crops in the world and also have used as a medicinal plant in Korea. We evaluated seed purity in F1-hybrid accessions using amplified fragment length polymorphism (AFLP) markers. One hundred sixty seeds from the male and female harvests were subsequently screened for seed purity with ten primers. The 13 accession-specific bands and many variable AFLP bands scored for accessions. Especially, E-AAC/M-CAA and E-AAG/M-CAT were presented clear hybrid bands for F1 hybrids. F1 hybrids maintained higher average level of genetic diversity compared with their correspondent parents. Self-inbred seeds from the female and male harvests were revealed 8.0% and 5.0%, respectively. The AFLP may lead to a better insight in to the hybrid seed purity test in I. tinctoria var. yezoensis.

벼멸구 생태형의 교잡종별 발육 및 수도 품종별 피해

최주수,박영도 한국생명과학회 1997 생명과학회지 Vol.7 No.4

Some attempts were made to investigate the gentic characteristics of the brown planthopper(BPH), Nilaparvata lugens Sta${\aa}$l, biotypes. In terms of egg and nymphal periods, egg hatcgability, emergence ratio, and population change of BPH and plant damage of rice cultivars by the infestation of BPH biotypes and their hybrid progenies in field conditions. The egg and nymphal periods of the three of BPH biotypes and their hybrid progenies on Dongjinbyeo with no resistance gene were shorter than on rice cultivars with resistance gene. But the periods of biotype-2(♀) X 2(♂) (E) on Samgangbyeo with Bph-1 gene and biotype-3(♀) X 3(♂)(I) on Mirying 63 with bph-2 gene were as short as on Dongjinbyeo. The egg hatchability and emergence ratio of the three Bph biotypes and their hybrid progenies on Dongjinbyeo were higher than on the other rice cultivars, and those of E on Samgangbyeo and I on Miryang 63 were as high as on Dongjinbyeo. In rice paddy field, biotype-1(♀) X 1(♂)(A)$\sim$I infested on Dongjinbyeo, E onSamgangbyeo, and I on Miryang 63 were increased their population densities remarkably in next generation. Dongjinbyeo infested by A$\sim$I, and Myryang 63 infested by I were hopperburned at 60 days after infestation and had a damage rating of 0.9. But Samgangbyeo infested by E was no hopperburn(damage rating: 7.3) though it showed high BPH population at 60 days after infestation.

Delaware 포도에서 Gibberellin 처리와 봉지씌우기가 과립의 성숙과 품질에 미치는 영향

최주수,박영도 한국생명과학회 1997 생명과학회지 Vol.7 No.4

This experiment was carried out to clarify the effects of gibberellin(GA) aplication and bagging on repeening and quality in 'Delaware' grape berries. Treatments are 4 plots(2X2 factorial experiment); GA, GA+bagging, bagging and control. The clusters were dipped twice in 100 ppm GA with GA treatment : 10 days before and after the full bloom. The results obtained as follows: 1. GA treatment made the seedless grape berry reduced in the fresh weight but it hastened the ripening period about 2 weeks. 2. Total soluble solid(TSS), viscosity and pH value of berry juice increased with maturation. The concentration of TSS and viscosity were higher in GA treatment plot than GA non-treatment. 3. Berry-hardness, titratable acidity and alcohol inslouble solid(AIS) decreased with maturation. Expically berry-hardness and AIS decreased more greatly in GA non-treatment than GA treatment. 4. The concentration of anthocyanin increased with ripening but pectic substance didn't fluctuate nearly. These of anthocyanin and pectin were higher in GA non-treatment plot than GA treatment. 5. By analysis of factorial experiment GA treatment was highly significant with the $^{o}$Brix/Acidity ratio, juice viscosity and AIS, but high negatively, significant with berry-hardness and berry fresh weight. And it was significant with T S S and negatively, titratable acidity. Bagging was significant with $^{o}$Brix/Acidity ratio and AIS content, but negatively, titratable acidity. 6. Qualitative characters were high correlated with the $^{o}$Brix/Acidity ratio in simple correlation but direct effect by the path-coefficient analysis didn't coincide with simple correlation. The direct effect of pH was large and juice viscosity, the next. And that of berry-hardness was negligible but, AIS, small negatively.

마늘 배양에 있어서 신초원기 유도

최주수,이복규,허만규,Choi Joo-Soo,Lee Bok-Kyu,Huh Man-Kyu 한국생명과학회 2006 생명과학회지 Vol.16 No.3

경제적인 채소류 중 향신료뿐만 아니라 약용으로서 이용 가치가 많은 마늘은 영양번식성 작물로 virus에 감염되어 있으므로, 조직배양에 의하여 마늘의 virus-free한 개체의 생산을 목적으로, 배양부위의 상위에 따른 배양효과의 차이와 호르몬 조성과 당의 농도가 조직배양의 생육 분화에 미치는 영향을 조사하였다. 또한 신초원기 유도를 위한 배양조건을 검토하였으며, 배양체의 조직학적 관찰을 행하였다. 치상부위 별로 보통엽 중하부조직이 배지조성에는 NAA 0.02 + BAP 1.0 mg/l 첨가배지가 발육과 분화에 다소 양호하였다. Shoot 분화에는 sucrose 40 g 첨가구에서 BAP의 증가에 따라 shoot형성이 다소 양호하였으며 NAA 0.02 + BAP 0.2 mg/l 첨가구에서는 가근이 유도되었다. 액체 회전 배양에서 신초원기라고 판단되는 조직이 유도되었다. Cultivated garlic, Allium sativum L. is economically important for leaves and bulbs, which historically were used in Korea for spices and condiments of Korean food as well as medicine crops. This experiment was carried out to investigate the effect of development and differentiation on culture of A. sativum (cv: white 6) by explant position, hormone composition and sucrose concentration in culture media. Culture method was investigated to induce shoot primordium. Culture efficiency was better with lower tissue of foliage leaf in explant position and on the medium with NAA 0.02 + BAP 1.0 mg/l in hormone composition than any other. Precocious shoot and callus were induced from shoot apex. Shoot was efficiently differentiated on 4,000 mg/l sucrose with increasing concentration of BAP. Shoot primordium was also induced with liquid rotary culture by histological observation. Rhizoid was induced from callus tissue cluster on medium with NAA 0.02 + BAP 2.0 mg/l.

벼멸구 생태형의 성장 특성별 감로 배설량의 차이

최주수,박영도 한국생명과학회 1999 생명과학회지 Vol.9 No.2

벼멸구(Nilaparvata lugens Stal)의 영기별, 시형별, 성별 및 교미유무별 우화경과일에 따른 감로배설량과 벼멸구의 실내사육충, 비래충 및 포장에서 2세대 경과된 충에 따른 생태형의 감로배설량을 조사한 결과 다음과 같다. 감수성품종 밀양 23호는 영기가 진행됨에 따라 감로배설량이 증가하여 5령기에는 1령기에 비해 6배이상 많았으며, 또 저항성품종 청청벼보다 2배이상 감로배설량이 많았다. 우화 후 경과일에 따른 감로배설량은 장.단시형 모두 우화 1일째는 적었으나, 우화 2일째부터 다소의 변이는 있었으나 증가하였다. 밀양 23호의 경우 교미한 장시형 암컷 성충의 감로배설량은 54.4$\textrm{mm}^2$로 교미하지 않은 장시형 암컷 성충의 43.6$\textrm{mm}^2$보다 높았고, 또한 교미한 단시형과 교미하지 않은 단시형 암컷성충은 각각 63.2$\textrm{mm}^2$, 55.2$\textrm{mm}^2$로 나타나 장시형에 비해 감로배설량이 많았다. 한편 암컷성충의 경우 장.단시형 모두 청청벼 보다 밀양 23호에서 약 3~4 배정도 높게 나타났다. 실내충, 비래충 및 포장충의 감로배설량은 생태형-1의 경우 밀양 23호에서 각각 76.0$\textrm{mm}^2$, 162.3$\textrm{mm}^2$, 118.3$\textrm{mm}^2$로 나타났으며, 생태형-2와 3도 실내충<포장충<비래충순으로 나타났다. 또한 생태형-2에 감수성인 청청벼에서의 실내충, 비래충, 포장충은 각각 42.1$\textrm{mm}^2$, 132.5$\textrm{mm}^2$, 113.5$\textrm{mm}^2$, 생태형-3에 감수성인 밀양 63호에서는 45.3$\textrm{mm}^2$, 251.4$\textrm{mm}^2$, 97.7$\textrm{mm}^2$로 밀양 23호에서와 같이 많은 감로배설량을 보였다. 그러나 각 생태형별 저항성품종에서는 실내충, 비래충, 포장충간에 감로배설량의 차이를 인정할 수 없었다. 따라서 벼품종에 대한 충체특성별 및 환경조건별로 가해능력의 차이가 있는 것으로 보아 앞으로 이러한 결과를 활용하면 벼멸구에 대한 저항성기작 연구와 벼멸구 생태 형들의 발생과 피해정도를 예측하여 벼멸구 방제에 중요한 자료로 활용될 수 있을 것으로 생각한다. Some attempts were made to investigate the honeydew excretion of the brown planthopper(BPH), Nilaparvata lugens Stal, biotypes in terms of instal nymph, days after emergence, macropterous and brachypterous mated and unmated female, and macropterous female with different sources fed on 60-day-old rice cultivars with different resistance genes. The feeding amount of the BPH biotypes was measured by using the honeydew excretion test. The feeding amount of fifth instar on Miryang 23 with no resistance gene was higher than that of Cheon-gcheongbyeo with Bph-1 gene by two times. Macropterous and brachypterous females showed low feeding amount at first day after emergence, but they showed high feeding amount from second days after emergence. Also the feeding amount of brachypterous female was higher than that of macropterous female. In addition, the feeding amount of BPH was in the order of nymph<macropterous male<brachypterous male<macropterous unmated female<macropterous mated female<brachypterous unmated female<brachypterous mated female. The feeding amount of the BPH biotype-1, 2, and 3 on Miryang 23 was in the order of laboratory(the insects reared for at least 10 generation in the laboratory)<field(the insects obtained from rice fields, 2 generation after migration)<migration(the insects caught directly from migrating population). The feeding of biotype-2 on Cheongcheongbyeo and biotype-3 on Miryang 63 with bph-2 gene was as high as that of biotype-1, 2, and 3 on Miryang 23.

벼멸구 생태형의 성장특성별 감로배설량의 차이

최주수,박영도 한국생명과학회 1998 한국생명과학회 학술발표회 Vol.20 No.-

온주 밀감 잎에서의 건물증가 및 전류에 미치는 기상요인의 영향

최주수 한국생명과학회 2001 한국생명과학회 학술발표회 Vol.33 No.-

Comparison of Mating Systems in Korean Populations of Oenanthe javanica

최주수,허만규 한국원예학회 2007 Horticulture, Environment, and Biotechnology Vol.48 No.4

The mating systems of natural populations of Oenanthe javanica in Korea were determined using allozyme analysis. For six natural populations, mean multi-loci estimate of outcrossing (tm) was 0.358 across 12 polymorphic loci. The expected inbreeding coefficient at equilibrium (Fe) was 0.476. For five cultivated populations, tm was 0.317 and the expected inbreeding coefficient at equilibrium (Fe) was 0.525. The tm indicates that the cultivated populations had lower values than those of wild populations, which may mean that the domestication process had eroded the level of outcrossing. In most cases, significant heterozygote deficiencies still occurred, suggesting that the presence of other forms of inbreeding in addition to consanguineous mating.

벼와 벼과 잡초에 따른 끝동매미충 유충의 발육 및 성충의 수명과 산란의 차이

최주수,박영도 한국생명과학회 2001 생명과학회지 Vol.11 No.2

Some attempts were made to investigate the biological characterisitcs of the green rice leafhopper(GLH) , Nepthotettix cincticeps Uhler in terms of nymphal development, emergence ratio. preovipositional period, growth index, adult longevity and fecundity fed on rice cultivar, Chucheongbyeo with no resistance gene and water foxtail, Alpecurus aequalis Sobol. var. amurensis Ohwi at 20 and $25^{\circ}C$ with a 16L;8D photoperiod. Development period of nymph on rice cultivar and water foxtail at $25^{\circ}C$ was shorter than that at 2$0^{\circ}C$ by twofold. The nymphal period of female was about one day longer than that of male. The mean emergence ratio of female and male on rice cultivar and water foxtail was 40.0, 76.7, 38.3, 73.3% at 20 and $25^{\circ}C$, respectively. Preovipositional period on rice cultivar and water foxtail at $25^{\circ}C$ was shorter that at 2$0^{\circ}C$ by twofold. Growth index on rice cultivar adn water foxtail at $25^{\circ}C$was higher than that at 2$0^{\circ}C$ by fourfold. Under the 20 and $25^{\circ}C$ condition, the longevity of female and male was longer on water foxtail than on rice cultivar, but the fecundity of GLH was higher on rice cultivar than on water foxtail. In conculsion, water foxtail seems to be adequate for spring host of GLH.

벼멸구 생태형의 성장 특성별 감로 배설량의 차이

최주수,박영도 동의대학교 기초과학연구소 2000 基礎科學硏究論文集 Vol.10 No.1

Some attempts were made to investigate the honeydew excretion of the brown planthopper(BPH), Nilaparvata lugens Stal, biotypes in terms of instar nymph, days after emergence, macropterous and brachypterous mated and unmated female, and macropterous female with different sources fed on 60-day-old rice cultivars with different resistance genes. The feeding amount of the BPH biotypes was measured by using the honeydew excretion test. The feeding amount of fifth instar on Miryang 23 with no resistance gene was higher than that of Cheongcheongbyeo with Bph-1 gene by two times. Macropterous females and brachypterous showed low feeding amount at first day after emergence, but they showed high feeding amount from second days after emergence. Also the feeding amount of brachypterous female was higher than that of macropterous female. In addition, the feeding amount of BPH was in the order of nymph$lt;macropterous male$lt;brachypterous male$lt;macropterous unmated female$lt;macropterous mated female$lt;brachypterous unmated female$lt;brachypterous mated female. The feeding amount of the BPH biotype-1, 2, and 3 on Miryang 23 was in the order of laboratory(the insects reared for at least 10 generation in the laboratory)$lt;field(the insects obtained from rice fields, 2 generation after migration)$lt;migration(the insects caught directly from migrating population). The feeding of biotype-2 on Cheongcheongbyeo and biotype-3 on Miryang 63 with bph-2 gene was as high as that of biotype-1,2, and 3 on Miryang 23.