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- 저자 : 최원재 ( Won Chae Choe ) (검색결과 2 건)
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실험연구 : 쥐 배아심장 H9c2세포의 허혈 후 재산소화 시 Propofol 농도가 활성산소 발생에 미치는 영향
김윤홍 ( Yun Hong Kim ),김현수 ( Hyun Soo Kim ),이영재 ( Young Jae Yi ),최원준 ( Won Joon Choi ),송준규 ( Jun Kyu Song ),이선민 ( Seon Min Lee ),최원재 ( Won Chae Choe ),김성수 ( Sung Soo Kim ) 대한마취과학회 2006 Korean Journal of Anesthesiology Vol.51 No.1
Background: Reoxygenation of an ischemic heart causes a decrease in the cardiac function, which is known as reperfusion injury that is associated with an increase in the concentration of reactive oxygen species (ROS). This study examined the effect of the propofol concentration on the generation of ROS during reoxygenation in rat embryonic heart H9c2 cells. Methods: Cultured H9c2 cells were examined in the following sequences: Prehypoxic, Hypoxic and Reoxygenation period. Each period required 60 minutes. The cells were exposed to propofol at the beginning of the prehypoxic period. Thirty minutes later, DCFH-DA (dichlorofluorescin diacetate) 10μM was added to detect the ROS. The propofol concentrations used were 0, 5, 25, 50, 250μM in the first experiment and 0, 1, 2, 3, 4, 5μM in the second experiment. The ROS level was estimated using a fluorometer at 5-minute intervals from 5 to 60 minutes after reoxygenation. Results: When the propofol concentrations was > 5μM, the ROS levels were significantly lower than those of the untreated group (P0) (P < 0.05). At propofol concentrations < 5μM, the ROS levels 35 to 60 minutes after reoxygenation were significantly lower that in the untreated group (P < 0.05). Between 5 and 30 minutes after reoxygenation, the cells exposed to 1, 4 and 5μM propofol also showed lower ROS levels than the untreated group P0. However, 2 and 3μM propofol did not show any significant difference in ROS values to those observed in the untreated group except for 2μM at 25 minutes after reoxygenation. Conclusions: During the reoxygenation period in H9c2 cells, propofol concentrations > 5μM inhibited ROS production over the whole period, and even 1μM showed some inhibition of ROS. (Korean J Anesthesiol 2006; 51: 89~93)
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세포성장 주기에 의한 S . pombe 의 dihydrofolate reductase 유전자의 발현 조절
김지영,노현모,최준호,최원재,정해정 한국유전학회 1995 Genes & Genomics Vol.17 No.1
We have previously isolated dihydrofolate reductase (DHFR) gene from fission yeast Schizosaccharomyces pombe by the phenotype of resistance to a folate analog, trimethoprim. One species of the DHFR transcript was identified by Northern blot analysis and was shown to be about 1,500 nucleotides in size. In order to examine regulation of the DHFR gene during cell cycle, we have analyzed the level of the DHFR mRNA in S. pombe cells in different growth phases, exponential or stationary phase, and in synchronized cells. The S. pombe DHFR mRNA level was higher in exponential phase than in stationay phase by four to five folds. The analysis of the DHFR mRNA from the synchronized cells has revealed that the level of DHFR mRNA increased by several folds on the onset of S phase, indicating that the expression of DHFR gene may be regulated at the transcriptional level during cell cycle in S. pombe as in the mammalian cells.
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