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장기간 진행하지 않는 인면역결필바이러스 ( Human Bmmunodeficiency Virus , HIV )-1 감염자로부터 분리한 HIV-1의 전체 염기서열 결정
조영걸(Young Geol Jo),이희정(Hee Jung Lee),Ronald C. Desrosiers 대한바이러스학회 1999 Journal of Bacteriology and Virology Vol.29 No.2
To characterize the molecular nature of human immunodeficiency virus (HIV)-1, we determined the full-length HIV-1 sequences from cultured peripheral blood mononuclear cells (PBMC) of a Korean long-term nonprogressor (LTNP). Without antiretroviral therapy, the individual has maintained CD4+ T counts over 500/㎕ from 1989 to 1999. Plasma viral RNA copy was 992 U/ ml in 1998. Culture supernatant showed positive from culture days 9. A series of 9 overlapping PCR products were amplified from cultured PBMC and cloned. About 9.2 kb from R of 5' LTR to R of 3' LTR was determined by automated sequencing. The G-to-A hypermutations were shown throughout the entire region. As a result of G to A hypermutations, premature stop codon was found in integrase coding region. Though there was no recombination between subtypes over all genomes, TATA box in both LTRs was TAAAA which is detected in subtype E instead of TATAA in subtype B. And, there were nucleotide GC insertion between NF-kB I and Sp1 III, and duplication of TCF-1a in LTR. We could not find any deletion of amino acid in Nef, Gag, Pol and Env gene. This study is the first report on molecular nature of full genomes of HIV-1 isolated in Korea.
인면역결핍 바이러스 pol 유전자 염기서열 결정에 의한 지도부딘 ( ZDV ) 내성 돌연변이의 탐지
조영걸(Young Geol Jo),이희정(Hee Jung Lee),성흥섭(Heung Suk Sung),김유겸(Yoo Gyum Kim),김영봉(Young Bong Kim),이용진(Yong Jin Lee),김미정(Mi Jung Kim),김대곤(Dae Gon Kim),원영호(Young Ho Won),조군제(Goon Je Jo) 대한바이러스학회 1999 Journal of Bacteriology and Virology Vol.29 No.4
The nested polymerase chain reaction (PCR) assay was used to determine the sequences of reverse transcriptase (RT) codons 41, 67, 70, 210, 215 and 219 of human immunodeficiency virus-1 (HIV-1) pol gene. Template DNA was obtained from uncultured peripheral blood mononuclear cells from 27 Korean HIV-1 infected patients treated with ZDV and Korean red ginseng. The second PCRs were done for 2 separated regions (RT codons 13-98 and 152 259) with 5 ㎕ of the first PCR product. Nucleotide sequences were determined by direct sequencing. In the 27 patients, CD4+ cell count decreased from 230±117/㎕ to 152±162/㎕ for 46±26 months (Mo), and actual duration of ZDV intake was 27±16 Mo. In the 16 patients who had been treated with ZDV therapy ≥25 Mo, the incidences of 70R, 215F/Y, and 41L were 61%, 28% and 22%, respectively and those of 67N, 210W and 219Q were 17%. The incidences of 215F/Y were 6.7% for group ≤12 Mo treatment, 22.7% for group with 13 to 24 Mo, and 27.8% for group ≥25 Mo. There was no mutation in 9 patients. It might be associated with the interruption of ZDV therapy for more than 6 months in 6 patients. This study shows that the detection of mutation could be useful prognostic marker with other clinical and virological data, and very low mutation rate is dectected compared to overseas reports.
담도 협착에서 종양 특성과 솔질세포검사 순서에 따른 진단 정확도
조영걸 ( Yeong Geol Jo ),이태훈 ( Tae Hoon Lee ),조현득 ( Hyun Deuk Cho ),박상흠 ( Sang Heum Park ),박재만 ( Jae Man Park ),조영신 ( Young Sin Cho ),정윤호 ( Yunho Jung ),정일권 ( Il Kwun Chung ),최현종 ( Hyun Jong Choi ),문종호 ( 대한소화기학회 2014 대한소화기학회지 Vol.63 No.4
Background/Aims: There are few data supporting the diagnostic yield of brush cytology depending on the order of cytologic reparation method or the location or shape of tumors in biliary strictures. We investigated diagnostic yields and variations n brush cytology with direct smear and cell-block preparations according to sampling preparation sequence and tumor location nd shape in biliary strictures. ethods: Patients who had undergone ERCP with tissue sampling between August 2009 and April 2013 were analyzed etrospectively. Group A was examined using brush cytology with direct smear followed by cell-block with or without biopsy, hile the reverse order was performed for group B. esults: Among 138 enrolled patients, 92 patients (A: 36, B: 56) underwent both brush cytology with direct smear and cell-block reparations. No differences in sensitivity, specificity, or accuracy were observed according to the sampling preparation method nd the location or shape of tumors in biliary strictures. The cellularity observed from brush cytology with direct smear was etter than that from cell-block according to the location of the tumor (p<0.01). The diagnostic yield was increased in both roups with addition of an endobiliary biopsy. onclusions: No difference in diagnostic accuracy was observed between the sequences of preparation for brush cytology ith direct smear and cell-block techniques. Brush cytology showed better cellularity for diagnosis.
장승호(Jang Seung-Ho),조영걸(Jo Young-Geol),도화용(Do Hwa-Yong),오지택(Oh Ji-Taek) 한국철도학회 2005 한국철도학회 학술발표대회논문집 Vol.- No.-
In this paper, the trend of the market demand for the technology of railway cars and the development direction of other country or company were studied by analysing the patent map. The effort for developing the technology is intensified from the middle of 1960s and it seems to be more strengthened especially in the parts of bogie, driving motor/power, window and door. Recently the increasing rate of the number of patent application from Germany and China is noticeable. The results of this paper can be used for making the development plans of competitive technologies and benchmarking of them.
정경원(Gyung Won Jung),남정현(Jung Hyun Nam),이호정(Ho Jung Lee),홍해남(Hae Nam Hong),조영걸(Young Geol Jo) 대한바이러스학회 2000 Journal of Bacteriology and Virology Vol.30 No.1
The aim of this study was to investigate viral etiology in dilated cardiomyopathy (DCM) by polymerase chain reaction (PCR) or nested reverse transcription PCR (RT-PCR), and characterize the enteroviral RNA presented in the clinical specimens. Twenty-eight paraffin-embedded heart tissue samples were assayed to detect cytomegalovirus, herpes simplex virus type 1, type 2, parvovirus, adenovirus, and enterovirus (EV) with each specific primer. Of these 28 patients (mean age: 27, M: 24, F: 4), 26 were histologically diagnosed as DCM and 2 as myocardial infarction (MI). Nested RT-PCR detected enteroviral RNA in 7 (26.9%) of 26 patients with DCM, and none of patients with MI. And none of DNA viruses tested were detected from the samples. Amplified products were also genotyped by single-strand conformation polymorphism (SSCP). Three subtypes can be differentiated from 7 clinical specimens. Furthermore, direct sequence analysis was performed to determine whether genetic variation of EV is present in the explanted heart tissues from patients with DCM. Although most of the sequences among the wild isolates have the greatest similarity to those of coxsackievirus B3, there are specific regions of variable sequences (no 490 - no 510). The data suggest that enterovirus may be a major viral pathogen for the DCM in Korea and nucleotide sequence data indicate that coxsackievirus B3 may be a leading etiologic agent of DCM.