해바라기씨중의 식용단백질에 관한 연구 -단백질의 분리 및 그의 화학적 조성에 관한 연구-

조성희,김준평,Cho, Sung-Hye,Kim, Jun-Pyong 한국식품과학회 1977 한국식품과학회지 Vol.9 No.2

한국에서 재배된 해바라기(Helianthus annus)씨 단백질의 화학적 성질을 규명하고자 이의 염용해성 단백질을 추출한 다음 정제하여 주단백질을 분리하여 본 결과 1. 염용해성 단백질의 추출용매는 10% 염화나트륨을 함유한 0.02M sodium phosphate buffer가 가장 좋았다. 2. 해바라기씨 단백질의 필수아미노산 조성은 비교적 양질인 것으로 나타났다. 3. 염용해성단백질의 disc전기이동에서 7개의 band를 나타내었다. 4. 염용해성단백질의 주단백질은 Sephadex G-150과 A-25 column으로 정제하여 얻을 수 있었다. 4. 주단백질의 분자량은 약 86,000이였다. We have investigated for amino acid composition and molecular weight of the sunflower main protein which was purfied by Sephadex column. The results were obtained as fellow. 1. The salt-soluble sunflower proteins were highly dispersible in 0.02M sodium phosphate buffer, containing 10% sodium chloride. 2. The sunflower proteins were characterized by comparatively high levels of essential amino acids. 3. Seven bands of component of sunflower proteins were found in disc electrophoretic gel column. 4. The sunflower main protein was purified by Sephadex G-150 and A-25 column chromatography 5. The molecular weight was estimated 86,000 for the sunflower main protein.

중-노년층 여성의 실버타운 개발유형에 대한 태도분석

조성희,강혜경,Cho, Sung-Heui,Kang, Hye-Gyoung 한국주거학회 2008 한국주거학회 논문집 Vol.19 No.3

The purpose of this study was to investigate the attitude of the middle and old age about the elderly housing and the planning elements according to the types of silver town. For this study, a pre-interview and a survey method were used for data collection. A questionnaire survey of 372 women over 45 years old was carried out. Among the data, 321 cases were analyzed by SPSS for Windows V12.0. The major findings were as follows: 1) Regarding the elderly housing, While the couple cohabitation type preferred the existing housing and the general housing, the single habitation type preferred the apartment and silver town. Some people wanted silver town occupancy due to the decrease of children support burden, the other did not want it due to economical burden and the absence of homelike affection. When they move into silver town, they wanted to take money, clothes, and photo album. 2) In silver town development, while the couple cohabitation type preferred the apartment, independent house, the single type preferred the apartment, housing complex and group-home. The unit housing sizes of them were around 82.6 and 49.6 square meters respectively. In the space composition of unit housing, while the couple cohabitation type preferred bath room, kitchen, living room and two of bed room, the single type preferred one of bed room and bath room at least. On the other hand, this study found out that it was possible to plan the space for eating and washing as the common space. 3) According to single or couple, and ages, there were significant differences in preferred spatial planning elements, which needs to be reflected in the silver town development. These results can provide improvement guidelines for the development of the silver town with the differentiation of design factors for the silver town planners.

한국산 밀가루 단백질의 화학적 조성에 관한 연구 (제2보) 주 Gliadin 단백질의 분자량 측정 및 말단아미노산의 검출

조성희,김준평,Sung Hye Cho,Jun Pyong Kim 대한화학회 1977 대한화학회지 Vol.21 No.3

장광품종의 밀 gliadin 단백질을 정제하여 주 gliadin 단백질을 얻은 다음 이의 아미노산조성과 분자량 그리고 N-말단 및 C-말단 아미노산을 조사하여 본 결과 1. 주 gliadin 단백질의 아미노산 조성은 glutamic acid의 함량이 가장 많았다. 2. 주 gliadin 단백질의 분자량은 60,200 ${\pm}$200이었다. 3. 주 gliadin 단백질의 N-말단 아미노산은 phenylalanine이었고, C-말단 아미노산은 methionine이었다. We have investigated for amino acid composition, molecular weight and terminal amino acid of the main gliadin protein which was purified by Sephadex column. The results were obtained as follows: 1. The highest content of amino acid was glutamic acid for the main gliadin protein. 2. The molecular weight was estimated 60,200 ${\pm}$200 for the main gliadin protein. 3. Identified N-terminal and C-terminal amino acid of the main gliadin protein were phenylalanine and methionine, respectively.

아파트 평면형태에 따른 발코니 공간의 사용행태에 관한 연구

조성희,박지혜,Cho, Sung-Heui,Park, Ji-Hye 한국주거학회 2007 한국주거학회 논문집 Vol.18 No.3

The purpose of this study was to investigate the using behavior of the balcony space in apartment housing for improving apartment balcony as dwelling space. For that, it is needed to identify the practical meaning of balcony space as function so balcony expansion and the using behavior of balcony space were investigated and analyzed. For this study, survey and depth-interview methods were used for data collection. The major findings were as followings: 1) Balcony expansion mainly occurred in balcony of livingroom and bedroom. Balcony expansion showed several phases according to the type of plan and function of balcony space and few residents chose expansion of all balconies. 2) The using behavior of balcony space was categorized by these various functions of balconies. It was also related to unit-plan type of apartment housing. These results can provide improvement guidelines for the balcony with the differentiation of design factors for residents.

고정된 효소의 Hysteresis 현상에 관한 연구

조성희,Cho, Sung-Hye 생화학분자생물학회 1983 한국생화학회지 Vol.16 No.2

고정된 단백질막중에서 확산되는 양성자의 이동에 기초를 둔 Eyring의 이론에 의하여 computer로 papain의 hysteresis 현상을 simulation하여 본 결과는 다음과 같았다. 즉 기질과 효소는 그들의 농도가 클수록 hysteresis를 잘 나타내었고 염과 완충용액은 이와반대로 그들의 농도가 적을수록 잘 나타내었다. 그리고 완충용액에 hysteresis 대한 현상은 염의 농도에 따라서 큰 영향을 받았으며, 이는 염을 첨가하였을때 보다 첨가하지 않은 상태에서 훨씬 더 잘 나타내었다. 이러한 현상이 실험적으로 동일한 결과가 나타날지는 실험적 연구가 뒷받침 되어야 할 것이다. Based on Erying's multibarrier activation process, a mathematical model was formulated to account for the proton diffusion through an enzyme attached at synthetic membrane. Then the phenomenon of enzyme hysteresis was tested by using computer-simulation with papain as an immobilized enzyme. The pH changes resulting from hysteresis appeared to occur markedly at higher concentration of the substrate (BSA) and enzyme, whereas at lower concentrations of salt and buffer, they appeared profoundly. Experimental support is needed to confirm the present results.

한국산 밀가루 단백질의 화학적 조성에 관한 연구 (제1보). Gliadin 단백질의 아미노산 조성 및 그의 정제

조성희,김준평,Sung Hye Cho,Kim Jun Pyong 대한화학회 1977 대한화학회지 Vol.21 No.3

한국산 밀 품종들 중에서 장광의 것을 선정하여 단백질을 추출한 다음 이 단백질의 아미노산 조성과 gliadin 단백질의 정제로 주 gliadin 단백질을 분리하여 본 결과 1. Gliadin과 glutenin 단백질은 다같이 disc 전기이동상에 5개의 band를 나타내었다. 2. Gliadin과 glutenin 단백질은 다같이 glutamic acid의 함량이 가장 많았다. 3. Gliadin과 glutenin 단백질간에 아미노산 조성의 차이는 gliadin이 glutenin보다 alanine의 함량은 적었으나 proline의 함량은 많았다. 4. 주 gliadin 단백질은 gliadin 단백질을 Sephadex G-150과 A-25 coumn으로 정제하여 얻었다. We have investigated the amino acid composition of gliadin and glutenin from wheat flour Jang Kwang variety and have purified the main gliadin protein by Sephadex column. The results were obtained as follows: 1. Five bands of component for both gliadin and glutenin were found in disc electrophoretic gel column 2. The highest content of amino acid in gliadin and glutenin were glutamic acid and alanine was present in comparatively large amounts in glutenin, whereas proline was rich in gliadin 3. Main gliadin protein was purified by Sephadex G-150 and A-25 column chromatography and identified its purity by disc electrophoresis.

산초(Zanthoxylum schinifolium) 추출물과 초피(Zanthoxylum piperitum) 정유의 꽁치과메기 산패 억제 효과

조성희(Sung-Hee Cho),권은혜(Eun-Hye Kwon),오승희(Seung-Hee Oh),우미희(Mi Hee Woo) 한국식품영양과학회 2009 한국식품영양과학회지 Vol.38 No.12

본 연구는 산초 잎의 에탄올 추출물과 초피 과피에서 얻은 정유를 과메기 제조 시 처리하였을 때 산패 억제와 품질 향상 효과를 조사하였다. 산초의 에탄올 추출물(ZS)은 1% 에탄올에, 초피 정유(ZP)는 20% 에탄올에 용해시켜 길이로 2등분하여 탈수시킨 꽁치 1 ㎏ 당 산초 추출물은 0, 0.125, 0.25, 1 ppm 되게, 초피 정유는 0, 0.25, 0.5, 1, 2 ppm 되게 분무하여 과메기를 자연 건조하여 제조하고 다층 필름포장제로 진공 포장하였다. 포장된 과메기를 실험 시까지 -20℃에 보관하였다가 개봉한 후 4℃ 냉장고에서 1, 3, 7일 보관하며 산패 정도와 아민류의 변화, 관능검사를 실시하였다. 과메기의 산가, 과산화물가 및 TBA값은 개봉 후 보관기간이 길어질수록 증가하였으며 산초 추출물 0.125 ppm 이상, 초피 정유 0.25 ppm에서 효과가 나타났으며 이 효과는 농도 의존적이며 보관기간이 길어질수록 더 뚜렷하였다. 산초 추출물과 초피 정유 처리에 의하여 과메기 내의 dimethylamine과 trimethylamine(TMA)의 증가도 억제하였으며 trimethylamine oxide의 함량은 높게 유지되었고 3일 보관한 과메기에서 TMA<4.5 ㎎/100 g을 유지시킬 수 있는 산초 추출물의 농도는 0.25 ppm, 초피 정유는 0.5 ppm이었다. 3일 보관된 과메기에 대한 관능검사 결과 초피정유를 0.5~1 ppm 처리한 시료에서 가장 좋은 점수를 나타내었다. 결론적으로 소량의 산초추출물과 초피 정유는 과메기의 산패를 억제하여 품질을 개선시키는 효과가 있다고 사료된다. The present study was conducted to investigate the effects of components obtained from Zanthoxylum schinifolium and Zanthoxylum piperitum on rancidity and quality of Kwamegi (semi-dried Pacific saury, Coloabis saira). Ethanol extract (ZS) of Zanthoxylum schinifolium leaves or the essential oil (ZP) obtained from pericarp of Zanthoxylum piperitum in 1 or 20% ethanol solution was sprayed to the Pacific saury before Kwamegi preparation at its final concentrations of 0.125~2 ppm in the Kwamegi. The prepared Kwamegi was vacuum packed with multi-layered film (polyethylene/polyamide/EVOH/polyethylene, thickness 80 ㎛) and kept at -20℃ until use. After opening the package Kwamegi was stored at 4℃ for 1, 3 and 7 days during which rancidity tests and sensory evaluation were carried out. Acid, peroxide, and thiobarbituric acid (TBA) values increased with storage time but reduced significantly by the addition of ZS at the concentrations of ≥0.125 ppm and ZP≥0.25 ppm. The effects of ZS and ZP were dose-dependent and more pronounced as storage time prolonged. The ZS and ZP also reduced dimethyamine and trimethyamine (TMA) contents which were increased with time, while they prevented the decrease of trimethyamine oxide. The ZS at the concentration of ≥0.25 ppm and the ZP at >0.5 ppm were needed to maintain TMA less than 4.5 ㎎/100 g for 3 day storage at 4℃. Sensory evaluation of the Kwamegi exhibited a slightly higher preference with the ZS and ZP treated ones at the level of 0.25~0.5 ppm. It is concluded that very low amounts of ZS and ZP are effective in suppression of rancidity of Kwamegi and could be utilized for its quality management.

아파트 거주가구의 프라이버시 조절 모델에 관한 연구

조성희(Cho Sung-Heui),박지혜(Park Ji-Hye) 대한건축학회 2009 대한건축학회논문집 Vol.25 No.7

The purpose of this study was to understand the privacy of apartment household. The investigators specifically focused on exploring privacy cognitive types and space using behavior of residents, in order to develop the privacy regulation model in an apartment complex. Surveys and in-depth-interviews were used for date collection. The questionnaire survey was conducted for housewives living in apartments in Busan. As a result, 514 cases were collected and analyzed. The results of this study are as follows: First, secrecy, control, expression, and safety were considered important facets of privacy while cognition regarding solitude and anonymity were relatively unimportant. Second, apartment residents showed ‘We-awareness’ toward their neighborhood within the apartment complex, and apartment residents believed that their privacy could be achieved by controlling social and physical boundaries of an apartment complex. Third, in terms of information regulation within the apartment complex, the apartment residents desired to appropriately control access of their information by using anonymity, different space, and certain behavior toward apartment and non-apartment residents. This study examined the basic characteristics of the privacy regulation mechanism of household in an apartment complex in Korea. The results provide a fundamental guideline for planning and designing residential spaces of the future.

주거 실내디자인에서 실내디자인 스타일의 인식연구

조성희(Cho, Sung-Heui),홍혜은(Hong, Hye-Eun) 한국실내디자인학회 2010 한국실내디자인학회 학술대회논문집 Vol.12 No.1

The objective of this study is to identify types of interior design styles recently appearing in model houses as well as characteristics by type. The study has been conducted based on both literature reviews and case studies. Research methodology includes the initial review of literature for interior design styles and the analysis of those interior design styles being applied to model houses. An expert group then classified the pictures of interior design of model houses into seven concepts to identify the traits of each style. The research is a fundamental study to review different perception of interior design styles between designers and the general public, which, in turn, is expected to provide a basic tool not only for understanding interior design styles, but also for guiding the future path of design.

한국인 총당류 섭취기준 설정

조성희(Cho Sung-Hee),정진은(Chung Chin-Eun),김선희(Kim Sun-Hee),정혜경(Chung Hye-Kyung) 韓國營養學會 2007 Journal of Nutrition and Health Vol.40 No.S

Sugars are a ubiquitous component of our food supply and are consumed as a naturally occurring component of many foods and as additions to foods during processing, preparation, or at the table. Most fruits and dairy products are high in sugars and thus naturally occurring sugars are consumed as part of a healthy diet. Some countries developed recommended daily intake figures (daily values: DVs or guideline daily amounts: GDA) for nutrients, and some countries, but not most have developed DV/GDA for total sugars. Dietary Reference Intakes for Koreans established by the Korean Nutrition Society in 2005, did not include the reference values for total sugar or added sugar. The committee on Dietary Reference Intakes for sugar was constituted in 2006 and discussed whether to specify added sugars or total sugar. Although added sugars are not chemically or physiologically different from naturally occurring sugars, many foods and beverages that are major sources of added sugars have lower micronutrient densities compared with foods and beverages that are major sources of naturally occuring sugars. But it was so hard to calculate a dietary intake of added sugar for Korean people, because there was insufficient information about contents of added sugar during processing or preparation of Korean food. Currently Korean or US food labels contain information on total sugars per serving but do not distinguish between sugars naturally present in food and added sugars. Therefore the committee decided to set the reference value for total sugar for Koreans. According to the recommended diet pattern for Koreans suggested by the Korean Nutrition Society, estimated sugar intake from the sugar containing food based on 2,000 kilocalories is 67g or 13% of total energy. Based on the data available on risk of obesity, hypertension, hyperlipidemia, insulin resistance, and metabolic syndrome from the analysis of Korean NHANES, it was insufficient evidence to set a UL for total sugar, but tended to increase serum LDL cholesterol and obesity at over 20-25% of energy from total sugar when consumed with high carbohydrates. Therefore the committee on Dietary Reference Intakes for sugar set the Acceptable Macronutrient Distribution Range for total sugar as 10-20% of total energy intake. (Korean J Nutr 2007; 40 (Suppl) : 3~8)