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This Study was conducted to estimate the degree of dominance and gene frequency of some sueful characters in tobacco. The eight parents and a set of 28 crosses of F'1s was F'2s were used as materials, and planted on oriental's and burley cultivated system as randomized block designs, respectively. The observed characters were six agronomic characters which were plant height, number of leaves per plant, leaf length, leaf width, days to flowering and yield, and the data obtained from the experiment were analyzed from methods by Hayman's and Jinks. The results obtained are summarized as follows: 1. In Vr-Wr graphical analysis, number of leaves per plant, leaf length, days to flowering and yield were found to be inherited in partial dominance, and plant height was over dominance to be similar to complete dominance, but leaf width was inherited with partial dominance close to complete dominance. 2. In the gene frequency, two varieties Xanthi-Basma and KA 102, for days to flowering and yield had larger number of dominant genes as those were situated near the point of origen. 3. Additive effects of genes(D) were greater than dominance effects of Genes(H) for six agronomic characters except plant height, and mean degree of dominance over all loci was lower than 1 for days to flowering yield, leaf length and number of leaves per plant.
The study was conducted to obtain the genetic informations for some useful characters in tobacco breeding. The eight parents and a set of 28 crosses of F1's and F2's were used as materials, and planted on two different cultivated systems, i. e., oriental's and burley systems, during 1986-1988 at taegu Experiment Station, Korea Ginseng & Tobacco Research Institute. The observed characters were six agronomic characters which were plant height, number of leaves per plant, leaf length, leaf width, days to flowering, yield and five chemical components, nicotine, total nitrogen, protein nitrogen, total volatile base and pet, ether extract. The results obtaining are summarized as follows: 1. The higher heritabilities were found for days to flowering yield and nicotine in both generations, but values for number of leaves per plant, leaf length, total nitrogen and protein nitrogen were lower than other characters. 2. Genotypic correlation coefficients among all pairs of characters were slightly higher than the corresponding phenotypic correlations in F1's and F2's on two different cultivated systems. The relationship between leaf length and leaf width was the positive correlation, but that between number of leaves per plant and leaf width was negative. 3. From the genotypic correlations between yield and other characters, a conclusion that the yield was highly correlated with plant height, leaf length, leaf width and days to flowering was given. 4. Quality was positively correlated with number of leaves per plant and nicotine, but negatively with the other agronomic characters and chemical components.
The field evaluation were conducted on dihaploid progenies derived from anther culture of F1 plant of Drama x Sohyang (N. tabacum L.) The mean values of agronomic and chemical traits of dihaploids such as Nicotine, length/width, plant height, Leaf length, leaves per plant and Yield were greater than those of tar parents. The correlation coefficient of dihaploids were similar to those of conventional lines and varieties. In path analysis, in relating yield components to yield, leaves per plant and leaf width had a direct effect on Yield, but the other components such as plant height, leaf length and days to flowering influenced Yield indirect way.
본 연구는 담배육종에 있어 유용형질이 담배 수량에 영향을 미치는 직접 및 간접효과들을 잡종초기세대에 분석함으로써 앞으로의 선발육종에 대한 기초정보를 얻기 위하여 수행하였다. 시험방법은 유전적 특성이 다양한 8개 품종을 공시하여 이면교잡을 하고 양성된 F1 , F2 각 세대별 28개 조합을 Orient 및 Burley 종 각 재배법에 따라 1986년부터 1988년까지 본 연구소 대구시험장에서 수행하였다. 조사항목은 초장, 엽수, 엽장, 엽폭, 개화일수 및 수량 등 6개 농업형질과 nicotine, total nitrogen, protein nitrogen, total volatile base, pet. ether extract 등 6개의 내용성분들로서 얻어진 결과는 다음과 같다. 1. 각 형질들의 수량에 대한 직접효과는 세대나 재배법에 따라 다소의 차이는 있었지만 대개 엽장, 엽폭, 개화일수 및 엽수의 순으로 높았으나 내용성분은 낮았다. 2. 간접효과는 재배법이나 세대에 관계없이 대개 엽장, 개화일수, 그리고 total nitrogen의 형질들이 타형질에 미치는 영향이 컸었다. The study was intended to clarify the direct and indirect effects of agronomic and chemical characters influencing upon yield in tobacco breeding. The eight parents and a set of 28 crosses of F1 's and F2 's were used as materials, and planted on two different cultivated systems, i.e., oriental's and burley systems, during 1986 to 1988 at Daegu Experiment Station. Four characters which were leaf length, leaf width, days to flowering and number of leaves per plant influenced the highly direct effects upon the leaf yield, but five chemical components were expressed as low effects. In indirect effects, leaf length, days to flowering and total nitrogen had the high indirect effects influencing upon leaf yield via the other characters. Accordingly, the results were discussed with a conclusion that the selection should be based on the data from leaf length, days to flowering, leaf width and number of leaves per plant.
Condensation heat transfer can be classified in dropwise condensation and filmwise condensation, and for the industrial purpose, the former is more useful than the latter because of the higher heat transfer rate. But it is difficult to maintain the dropwise condensation continuously since most of the metal surfaces become wetted after exposure to a condensing vapor over an extended period of time. To maintain dropwise condensation continuously , various surface coatings and promoters have been used recently, but these methods must be reconsidered about the durability of condensing surface. Therefore, in this study, evaporating method of various pure metals on the condensing surface has been performed to maintain dropwise condensation. The results have showed that the heat transfer rate of silver evaporating surface is higher than any other metal evaporating in dropwise area. Transition temperature and filmwise condensation curves are uniform regardless of kinds of evaporating metals.
We constructed a H. pylori expression vector which consisted of both a His-tag and a GST tag as purification toolsfor recombinant protein and a chloramphenicol resistant catgene as a reporter. The backbone of the vector pBK contained an ColEI origin of replication and a kanamycin resistantgene. A set of oligos for the His-tag and the PCR product of gst (glutathione S-transferase) gene were inserted sequentiallyin frame in themulti-cloning site of pBK. The orf of cat was inserted downstream of the gst to generate pBKHGC. The 3' part of H. pylori clpB and flaA were cloned into the vector which was introduced into H. pylori. Recombinant proteins were purified by GSH affinity column, digested with thrombin and were analyzed by western blotting. The final recombinant proteins were successfully purified.