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2-Aminobenzothiazole 유도체의 합성 및 항균작용에 관한 연구
정상헌,정원근,정필근,이남복 대한약학회 1976 약학회지 Vol.20 No.1
Sixteen compounds of 2-aminobenzothiazole derivatives were synthesized from alkyl ($C_{1-5}$) p-aminosalicylate by thiocyanation reaction. The NMR spectra of synthesized compounds showed that they were actually mixture of 5-hydroxy-6-alk-oxycarbonyl-2-aminobenzothiazole [alkoxy=methoxy(Ia), ethoxy (IIa), n-propoxy (IIIa), iso-propoxy (IVa), n-butoxy (Va), iso-butoxy (VIa), n-amoxy (VIIa), iso-amoxy(VIIIa)] and 7-(1b), ethoxy(IIb), n-propoxy(IIIb), iso-propoxy(IVb), n-butoxy(Vb), iso-butpxy (VIb), n-amoxy (VIIb), iso-amoxy (VIIIb)]. The mixtures of two isomeric benzothiazole were separated by two isomers varied with the kind of alkyl chain in alkyl p-aminosalicylate. These compounds were subjected to the test for antimicrobial activities using Staphylococcus aureus and Escherichia coli by tube dilutioin method. The seven compounds, Ia, IIa, IIIa, VIa, IIIb, IVb and Vb showed inhibition of the growth of S. aureus at the concentration of 10${\mu}$g/ml. As to the growth of E. coli, IVb, VIb, VIIb, and VIIIb were observed inhibition at the concentration of 1${\mu}$g/ml. Ia, IIa, IIIa, VIIa, Va, VIIIa, and IIb exhibited potential antimicrobial activities against showed inhibition of the growth of E.coli at the concentration of 100${\mu}$/ml.
Lectobacillus sporogenes에 의한 $\beta$-Galactosidase 생산에 관한 연구 ( I ) -균체외 $\beta$-Galactosidase의 생산 -
김영만,이정치,정필근,최용진,양한철 한국미생물 · 생명공학회 1983 한국미생물·생명공학회지 Vol.11 No.1
유포자 유산균인 Lactobacillus sporogenes 가 특이하게 균체외 $\beta$-galactosidase를 다량 생산하는 것을 발견하여 효소생산을 위한 영양요구성과 배양조건을 조사하였다. 영양요구성으로는 탄소원으로서 lactose 1%, 유기질소원으로서 peptone 1.5%, 무기질소원으로서 ammonium sulfate 0.2%, phosphorus 원으로 ammonium phosphate, dibasic을 0.8%. mineral은 potassium chloride 0.05%, ferric chloride 0.001% 첨가했을 때 최대의 효소생산을 나타내었다. 배지의 최적 initial pH는 7.0, 최적배양온도는 37$^{\circ}C$, aeration효과는 500$m\ell$용 진탕 flask에 배지량을 50~200$m\ell$ 주입, 140 strokes/min (진폭 7cm)으로 진탕 배양하였을 때 최고의 효소생산을 나타냈다. 상기의 최적조건에서 균체증식은 24시간 배양에 최고에 달하는 반면 $\beta$-galactosidase 생산은 균체내 효소생산의 경우 균체증식 속도와 비례적으로 증가하여 배양 24시간 전후에서 30U/$m\ell$로 최고치를 나타내고 균체외 효소는 배양 40시간 전후 즉, 균증식의 후기 정지기에서 38U/$m\ell$로 가장 높은 효소생산량을 보였다. 또한 fermentor 실험 역시 flask배양과 거의 비슷한 배양 양상을 보였으며 균체외 효소역가는 45U/$m\ell$로 진탕 flask 배양 결과보다 다소 양호하였다. Cultural conditions for the production of extracellar $\beta$-galactosidase by Loctobacillus sporogenes, a spore forming lactic acid bacterium, were investigated with shaken flask and jar fermenter cultures. The fermentation medium giving maximum $\beta$-galactosidase yield was found to consist of 1 % lactose as a carbon source, 1.5% peptone as an organic nitrogen source. 0.2% ammonium sulfate as an inorganic nitrogen source, 0.8% ammonium phosphate dibasic as a phosphorus source, and 0.05% potassium chloride and 0.001% ferric chloride as mineral source. Optimal initial pH of the medium was 7.0 and the highest enzyme excretion was observed after 40 hours of cultivation at 37$^{\circ}C$. In this experiment, the 500$m\ell$ conical flask containing 50-200$m\ell$ of medium was shaken at 140 strokes per minute with 7cm amplitude in a reciprocating shaker. The maximum enzyme value attained was 38 U/$m\ell$ of the culture broth which was found to be slightly higher than the highest intermolecular enzyme activity (30 U/$m\ell$) observed after 24 hours of incubation. In the fermentor culture, the fermentation profile was shown to be similar to that observed in the shaken flask experiment. But the maximum extracellular enzyme activity was 45 U/$m\ell$ to be even higher than the value obtained with the shaken flask culture.
Benzoxazolin-2-thione과 PAS의 Alkyl Ester과의 Mannich Base 합성 및 항균력에 관한 연구
정원근(Won Keun Chung),정상헌(Sang Hun Chung),정필근(Pil Keun Chung),윤원영(Won Yung Yoon),이남복(Nam Bok Lee) 대한약학회 1974 약학회지 Vol.18 No.4
Nine benzoxazolin-2-thione derivatives were synthesized as the potential antimicrobial substances. These compounds were tested for the antimicrobial activities using Staphylococcus aureus and Escherichia coli by way of tube dilution method. The three compounds of 1, II and VII inhibited the growth of S. aureus at the concentration of 1mcg/ml, and III, VI, VIII and IX exhibited considerable antimicrobial activities against S. aureus at the concentration of l0mcg/ml. As to the growth of E. Coli, VII VII and VIII inhibited at the concentration of 1mcg/ml. II,III and IV exhibited considerable antimicrobial activities against E. coli at the concentration of 10mcg/ml.
Lactobacillus sporogenes에 의한 혈중 Cholesterol의 저하
석은경(Eun Kyung Seok),김태한(Tae Han Kim),이정치(Jung Chi Lee),정필근(Pil Keun Chung),이금기(Kum Ki Lee) 대한약학회 1987 약학회지 Vol.31 No.5
The increase in the serum cholesterol level of the rabbit fed a high cholesterol diet was prevented by the administration of a Lactobacillus sporogenes preparation, proportionally to the amount of the preparation. About 90% of the increase, in the serum cholesterol level was inhibited in the rabbits fed the preparation (2%) as feed additives. In vitro, L. sporogenes cultured in MRS broth containing PPLO serum fraction as a cholesterol source decreased the cholesterol concentration in the spent broth and increased the intracellular cholesterol concentration. The ability of L. sporogenes to accumulate cholesterol was enhanced by the presence of oxgall in the medium. These results suggest that L. sporogenes may directly assimilate the cholesterol in the gastrointestinal tracts and may prevent the increase of the serum cholesterol level.
한인규(I . K . Han),이상철(S . C . Lee),이진희(J . H . Lee),김정대(J . D . Kim),정필근(P . K . Jung),이정치(J . C . Lee) 한국축산학회 1984 한국축산학회지 Vol.26 No.2
This experiment was conducted for a period of 8 weeks in order to study the effects of Clostridum butyricum ID on the growing performance and the changes in microbial flora of the feces and intestinal contents of broiler chicks. A total number of 200 one day old broiler chides was employed into five treatment groups consisted of control, Cl. butyricum ID 0.02%, Cl. butyricum ID 0.05%, Cl. butyricum ID 0.1% and Zn-bacitracin 0.05% for the feeding trial, metabolic trial and microbial assay. The results obtained were summarized as follows: 1. Body weight gain of chicks fed Cl. butyricum ID at the level of 0.05% and 0.1% was significantly(p $lt; 0.05) higher than that of control, and especially that of chicks fed Cl. butyricum ID at level of 0.05% were the highest among all treatments. No significant differences were found on feed intakes between treatments. Feed efficiency was remarkably(p $lt; 0.05) improved by the chicks fed Cl. butyricum ID at the level of 0.05%, 0.1% and Zn-bacitracin at 0.05% level, and also slightly improved by the chicks fed Cl. butyricum at 0.02% level compared to control. 2. Significant differences were not detected in intestinal pH change among treatments. Although ammonia concentration in ceca was reduced by the addition of Cl. buryricum ID, no difference was found among the levels of addition. 3. The present data revealed that the nutrient utilizability of experimental diets, although nonsignificant, was slightly improved by the addition of Cl. butyricum ID. 4. The supplementation of Cl. butyricum ID to the broiler chick diets caused sharp change in microbial flora counts in the feces after 3 weeks. Counts of such microbial flora as Cl. butyricum and Lactobacilli in feces were greatly increased, but those of Staphylococci and Coliforms were decreased by the addition of Cl. butyricum ID. The addition of Zn-bacitracin caused a little reduction in the counts of Staphylococci and Coliforms compared to control group. 5. As would be expected, it was also found that the counts of Cl. butyricum and Lactobacilli in the large intestinal contents were greatly increased, but those of Staphylococci and Coliforms were decreased by the addition of Cl. butyricum ID. In final, it may be concluded that the supplementation of Cl. butyricum ID for broiler chick diets could improve the weight gain and feed efficiency through the reduction in intestinal ammonia concentration and prevention of diseases possibly caused by the injurious microbial flora in intestine. Therefore, it may be suggested that Cl. butyricum ID can be used as an effective feed additive for growth promotion of broiler chicks. Further studies would be necessary to examine if L. butyricum would exert any beneficial effects on the performance of laying hens.