두릅의 휘발성 향기성분에 관한 연구

정태영(Tae Yung Chung),김소미(So Mi Kim) 한국응용생명화학회 1996 Applied Biological Chemistry (Appl Biol Chem) Vol.39 No.5

The volatile concentrate obtained from the edible Korean dureup plant (Aralia elata Seem.) by a distillation-extraction system was separated into hydrocarbon and oxygen-containing fractions, and the latter was further separated into nine subfractions by silica gel column chromatography. Gas chromatography (GC) and gas chromatography/mass spectrometry (GC/MS) were utilized to identify 167 volatile compounds in the fractions. The volatile compounds included 72 hydrocarbons, 31 alcohols, 23 aldehydes, 16 esters. 10 acids. 6 ketons, 3 furans, 2 phenols. 1 indole. 1 oxide, 1 sulfide, and 1 lactone. β-Caryopyllene, a sesquiterpene hydrocarbon, was the most abundant volatile compound identified in Korean dureup (19.53%). Dureup oil was found to possess a woody or herbaceous aroma following sensory evaluation of each fraction and individual volatile component using a GC-sniff apparatus.

진달래꽃으로부터 분리된 플라보노이드 화합물의 항산화성에 관한 연구

정태영(Tae Yung Chung),김미애(Mi Ae Kim),(A . Daniel Jones) 한국응용생명화학회 1996 Applied Biological Chemistry (Appl Biol Chem) Vol.39 No.4

Seven antioxidative flavonoids were isolated from Jindalrae flowers (Rhododendron mucronulatum Turcz.), an edible plant in Korea. These compounds were identified as afzelin, ampelopsin, catechin, myricetin, myricitrin, quercetin and quercitrin on the basis of IR, UV, FAB-MS, ¹H NMR, and ^(13)C NMR data. These compounds were consisted of two flavonols, three flavonol glycosides, a flavane, and a dihydroflavonol. The flavonol glycosides (14.4 g) present in both ethyl ether and ethyl acetate fractions comprised up to 82% of their total flavonoid amount (17.6 g) finally recovered by means of polyamide C-200 column chromatography. preparative TLC, recrystallization, and Sephadex LH-20 column chromatography. The antioxidant activities were measured in an ethanol solution of linoleic acid in the presence of ferric thiocyanate. The antioxidant efficiency increased in the order of afzelin$lt;α-tocopherol$lt;catechin$lt;quercitrin$lt;quercetin$lt;myricitrin$lt;ampelopsin$lt;myricetin.

Volatile Flavor Components in Chinese Quince Fruits, Chaenomeles sinensis koehne

Tae-Yung Chung(정태영),Dae-Sun Cho(조대선),Jae-Chul Song(송재철) 한국식품과학회 1988 한국식품과학회지 Vol.20 No.2

Constituents of the DRIED TOMATO FRUITS (Lycopersicon esculentum, Mi Soo)

정태영(Tae Yung Chung),早瀨文孝(Fumitaka Hayase),紘谷明沖(Akihiro Okitani),加藤博通(Hiromichi Kato) 한국식품영양과학회 1987 한국식품영양과학회지 Vol.16 No.3

건조 토마토의 향미성분과 관련있는 성분조성을 알고자 무기질, 당, 유리형 아미노산, 핵산 및 지질등의 불휘발성 성분을 분석 정량하였다.<br/> 회분의 주요성분은 칼륨, 칼슘 및 인이며, 카드뮴과 납은 극소량함유 되었고, 당류에서는 glucose와 fructose가 주성분이었으며, fructose가 glucose보다 약간 함량이 높았다.<br/> 총 유리 아미노산의 함량은 8322.5㎎%이였고, 주요 아미노산은 asparagine, aspartic acid, urea, glutamic acid, β-alanine 및 γ-aminobutyric acid로서, 총 유리 아미노산의 58.01%를 차지하였다.<br/> 핵산과 관계되는 물질은 CMP, UMP, GMP 및 IMP였으며, 이중 CMP가 전체의 약 58.07%로서 가장 함량이 높았고, IMP가 가장 낮은 함량을 보였다.<br/> 총지질로 부터 얻어진 검화물 중의 지방산의 주성분은 C_(18:2), C_(18:1), C_(16:0)이었으며, 불검화물중의 주요성분은 stigmasterol 및 β-sitosterol 이었다. For surveying of constituents concerning the flavor of the dried tomato fruits, nonvolatile components such as minerals, sugars, free amino acids, nucleotides and lipids were analyzed and determined.<br/> Potassium, calcium and phosphorus were the major constituents of ashes, but trace amounts of cadmium and lead were determined.<br/> Glucose and fructose were the major constituents in sugars, and the latter slightly showed higher value than the former.<br/> The content of total free amino acids was 8322.5㎎%, and that of the major components such as asparagine, aspartic acid, urea, glutamic acid, ß-alanine and γ-aminobuthyric acid showed 58.01 % to the total amount.<br/> The nucleotides were composed of CMP, UMP, GMP and IMP, and CMP among them showed the highest value as about 58.07% to the total amount. On the other hand, IMP showed the lowest one.<br/> The major components of the total fatty acids from the saponifiable fraction in the lipids were C_(18:2), C_(18:1) and C_(16:0), and those of the total sterols from unsaponifiable one were stigmasterol and ß-sitosterol.

진달래꽃으로부터 분리된 페놀산 화합물의 항산화성에 관한 연구

정태영(Tae Yung Chung),김미애(Mi Ae Kim),(A . Daniel Jones) 한국응용생명화학회 1996 Applied Biological Chemistry (Appl Biol Chem) Vol.39 No.6

Six phenolic acids were isolated from Jindalrae flowers (Rhododendron mucronulatum Turcz.), an edible plant in Korea. These compounds were identified as chlorogenic acid, 3,5-O-dicaffeoylquinic acid, 4,5-O-dicaffeoylquinic acid, caffeic acid, ferulic acid, and p-coumaric acid on the basis of IR, UV, ¹H and ^(13)C NMR, FAB-MS, ES-MS and/or EI-MS data. Chlorogenic acid (0.2 g) present in both ethyl acetate and ethyl ether fractions comprised up to 38.5% of the total phenolic acid amount (0.52 g) finally recovered by means of polyamide C-200 column chromatography, preparative TLC, recrystallization, and Sephadex LH-20 column chromatography. The antioxidant activities were measured in an ethanol solution of linoleic acid in the presence of ferric thiocyanate. The antioxidant efficiency increased in the order of p-coumaric acid$lt;α-tocopherol$lt;ferulic acid$lt;caffeic acid$lt;chlorogenic acid$lt;4,5-dicaffeoylquinic acid$lt;3,5-dicaffeoylquinic acid.

Nonvolatile Flavor Components in Chinese Quince Fruits, Chaenomeles sinensis koehne

Tae-Yung Chung(정태영),Dae-Sun Cho(조대선),Jae-Chul Song(송재철) 한국식품과학회 1988 한국식품과학회지 Vol.20 No.3

참취 분말이 에탄올을 투여한 흰쥐의 항산화계에 미치는 효과

이승은(Seung Eun Lee),성낙술(Nak Sul Seong),정태영(Tae Yung Chung),최미연(Mie Youn Choi),윤은경(Eun Kyong Yun),정유진(Yu Jin Jeung) 한국식품영양과학회 2001 한국식품영양과학회지 Vol.30 No.6

In vitro 항산화 활성이 확인된 참취가 생체 내에서도 활성을 나타내는 지를 확인하기 위해 대조군과 5% 및 10% 참취분말을 첨가한 식이를 4주간 흰쥐에 투여하면서 마지막 1주간 알콜로 산화적 스트레스를 유발한 후 희생시킨 흰쥐의 체중 증가량과 함께 체중에 대한 장기의 상대 중량, 간, 신장에 서의 지질과산화, 항산화효소 활성, glutathione 및 혈청 알부민의 함량을 분석하였다. 4주간의 사육 후 체중 증가량은 대조군, 5% 참취첨가군 그리고 10% 참취첨가군의 순으로 높았으나 유의적인 차이는 없었으며 체중에 대한 상대적인 장기 중량 중에서 간, 비장은 대조군에 비해 참취 첨가군의 경우 더 낮은 비율을 나타내었다. 간에서 지질과산화로 생성되는 TBARS 함량은 대조군(66 μg)에 비해 5%(46 μg) 및 10% 참취 분말 식이군(35 μg)에서 참취첨가량이 증가할수록 낮았다. Mn-SOD와 catalase 활성은 참취 첨가군들에서 대조군보다 낮았으나 Cu, Zn-SOD와 glutathione peroxidase 활성 및 glutathione 함량은 실험군들간에 유의적인 차이를 나타내지 않았다. 신장에서는 Mn-SOD와 glutathione peroxidase 활성이 대조군보다 참취 첨가군에서 유사하거나 낮은 경향을 나타내었으나 Cu, Zn-SOD와 catalase의 활성 및 TBARS, glutathione의 함량은 실험군들간에 유의적인 차이를 보이지 않았다. 한편, 혈청 알부민의 함량은 실험군들간에 큰 차이가 없었다. 이상과 같은 실험결과를 종합할 때 참취는 에탄올에 의해 유발된 산화적인 스트레스를 적절하게 방어하여 특히 간장에서의 지질과산화 및 Mn-SOD 활성의 감소를 가져왔으며 그 효과는 참취의 첨가량에 비례하는 경향을 나타내었으므로 생체 안에서 알코올과 같은 산화적 스트레스에 대한 항산화제로서의 역할을 효과적으로 수행할 수 있을 것으로 기대된다. The present study was conducted to investigate effect of powdered herb of Aster scaber Thunb. (chamchwi) on antioxidant system in ethanol-administrated rats. Four week-old Sprague Dawley male rats which had initial body weights of 97.10±4.50 g were randomly divided into three groups: control (ethanol treated, vitamin E-deficient group); 5% chamchwi (ethanol-treated, 5% chamchwi powder-supplemented group): 10% chamchwi (ethanol-treated, 10% chamchwi powder-supplemented group). Three groups of rats were supplemented with three experimental diets for 4 weeks and orally administrated 10% ethanol (v/v) daily via drinking water in the last experimental week. Contents of TBARS (thiobarbituric acid reactive substance), glutathione in liver and kidney and serum albumin were determined. The activities of superoxide dismutase (SOD), catalase and glutathione peroxidase (GSH-px) in liver and kidney were also analyzed. Relative weight of liver and spleen to body in chamchwi groups was lower than that in control group (p<0.05). The most remarkable result was that liver TBARS contents in chamchwi groups (5% chamchwi group, 46 μg in MDA value; 10% chamchwi group, 35μg) were significantly lower (p<0.05) than that in control group (66 μg). The supplement of chamchwi powder lowered the activity of manganese-superoxide dismutase (Mn-SOD), catalase in liver and GSH-px in kidney. The levels of glutathione in liver and kidney and serum albumin were not significantly different in all experimental groups (p<0.05). These results indicate that powdered herb of Aster scaber decreases lipid peroxidation and acitvity of Mn-SOD increased by alcohol-induced oxidative stress in liver of rats.

표고버섯의 열수추출 다당류가 발암원을 첨가급여한 흰쥐의 지질 대사에 미치는 영향

최미연(Mie-Youn Choi),임상선(Sang-Sun Lim),정태영(Tae-Yung Chung) 한국식품영양과학회 2000 한국식품영양과학회지 Vol.29 No.2

표고버섯의 열수추출물과 발암원인 butter yellow를 6주동안 흰쥐에게 급여한 후 간 및 혈장 의 지질성분 분석 및 간 기능에 미치는 영향을 검토한 결과는 다음과 같다. Butter yellow 단독 투여군인 BO군에서는 기본식이만을 준 대조군(NO)에 비하여 식욕 감퇴와 체중이 감소한 반면, 표고버섯의 열수추출물(PS) 급여군인 NP군 및 BP군에서는 PS 비급여군인 NO군과 BO군에 비하여 체중 증가량이 현저히 높았다. 간의 총 지질, 총 콜레스테롤, 중성 지질 및 인지질의 함량은 PS투여군이 대조군에 비하여 유의적으로 낮았으나, butter yellow 첨가군(BO)에서는 NO군과 비교하여 인지질은 비슷한 수치였으며, 총지질 및 총 콜레스테롤은 유의적으로 높았다. 혈장의 중성지질과 총 콜레스테롤 함량 또한 BO군에서 지질성분들의 수치가 유의적으로 높았고 PS의 투여로(BP군) 중성지질과 총 콜레스테롤 농도가 감소되는 효과가 있었다. 이상의 결과를 종합해 볼 때 표고버섯 열수추출 다당류(PS)는 화학적 발암물질인 butter yellow 투여시에 나타나는 지질대사 이상으로 인한 지질의 상승을 방지하며 이러한 결과는 PS의 지질과산화에 대한 방어 작용 또는 콜레스테롤 저하작용을 하는 성분에 의한 것으로 추측되며 이러한 혈장과 간의 중성 지질 및 콜레스테롤 농도의 상승을 방지하는 작용의 물질은 동맥경화증을 예방할 수 있으며, 이는 지질과산화로 인한 지단백질의 변화에 대하여 방어작용과 세포, 면역 수준에서의 부수적 효과를 배제할 수는 없는 것으로 사료된다. 또한 PS의 투여로 발암물질을 투여하지 않았을 때와 유사한 수치를 나타남으로서, 이 분획물들 중에 함유되어 있는 유효 성분이 간장의 해독기구에 영향을 주어 간 기능을 활성화 하거나 또는 지방축적 및 고지질화에 완만한 개선작용을 나타내고 있음을 알 수 있었다. The effects of hot water soluble polysaccharides extract (PS) of Lentinus edodes on the lipid components in the liver and plasma of the rats fed the butter yellow were investigated. Twenty four rats were divided into four groups and fed with the diet containing 15% casein, 30% sucrose and 10% soybean oil (basal diet; NO group), supplemented with butter yellow (BO group) or/and PS (NP, BP group). The contents of triglyceride and phospholipid in liver were significantly lower in BP group than BO group but the content of total lipid and total cholesterol were not significant difference between BO and BP. The concentrations of total cholesterol and triglyceride of plasma were significantly lower in BP than BO. As the results PS of Lentinus edodes prevent the triglyceride and cholesterol rise and then improve the lipid metabolism of rats fed the butter yellow.

땅콩 품종간 항산화 활성 비교

이승은(Seung-Eun Lee),박장환(Chang-Hwan Park),방진기(Jin-Ki Bang),성낙술(Nak-Sul Seong),정태영(Tae-Yung Chung) 한국식품영양과학회 2004 한국식품영양과학회지 Vol.33 No.6

기능성 소재로의 이용성을 검토하기 위해 9가지 땅콩 품종의 종실, 종피 및 종실체에 대한 항산화 활성을 비교하였으며 그 결과는 다음과 같다. 사람의 저밀도지단백(LDL)의 산화에 대해 종실, 종실체 및 종피는 각각 85.3~91.0%, 77.4~85.8%, -1.7~46.1%의 저해활성을, linoleic acid 산화에 대해 각각 94.0~94.9%, 94.2~95.0%, 69.4~80.2%의 활성을 나타내었다. 각 품종별 LDL 산화저해능은 보원땅콩, 미광땅콩, 신대광땅콩 및 기풍땅콩이, linoleic acid 과산화 저해활성은 신대광, 미광, 대풍, 왕, 기풍 등의 품종이 비교적 우수한 효과를 나타내었다. Superoxide anion radical에 대한 소거활성을 분석한 결과 종피(88.3~98.7%), 종실체(16.7~56.3%) 및 종실(-28.0~20.0%)의 순으로, DPPH 라디칼에 대해서는 종피 (89.9~90.8%), 종실체(6.0~12.0%) 및 종실(0.6~2.1%)의 순으로 높게 나타났다. 품종별 superoxide anion 라디칼 소거능은 대신땅콩, 대광땅콩, 기풍땅콩, 새들땅콩, 대풍땅콩 등이, DPPH 라디칼 소거능은 대광땅콩, 대신땅콩, 새들땅콩 및 기풍땅콩 등이 비교적 우수한 활성을 나타내었다. 총페놀 함량에서는 종피, 종실체, 종실이 각각 12.7~21.0 mg%, 2.46~4.24 mg%, 0.94~1.16 mg%의 함량을 나타내었으며 대광, 대신, 보원, 새들, 기풍 등의 품종이 상대적으로 높은 함량을 나타내었다. 품종 간의 차이는 LDL 산화저해에 대해 종피가, DPPH 라디칼에 대해서는 종실이, superoxide anion radical에 대해 종실체가, 총페놀 함량에서는 종피가 비교적 큰 것을 확인할 수 있었다. 이러한 항산화 활성 및 총페놀 함량을 종합할 때 대광, 대신, 기풍, 새들 등의 땅콩 품종이 비교적 우수한 결과를 나타내었다. To increase utilities as functional materials, nine peanut varieties were evaluated on antioxidant activity and total phenol content. Peanut varieties were individually divided into three parts such as part without seed coat, seed coat, and seed. Each of the 80% ethanol-extracted solutions from the parts was used for the assay. Inhibitory activities on low density lipoprotein (LDL) oxidation by the part without seed coat, seed coat, and seeds from 9 varieties were respectively 85.3~91.0%, 77.4~85.8%, -1.7~46.1%, and those on linoleic acid oxidation were 94.0~94.9%, 94.2~95.0%, 69.4~80.2%, respectively. Seed coats, parts without seed coat, and seeds from 9 peanut varieties showed scavenging activities as 88.3~98.7%, 16.7~56.3%, -28.0~20.0% on superoxide anion radical and as 89.9~90.8%, 6.0~12.0%, 0.6~2.1% on 1,1-diphenylpicrylhydrazyl (DPPH) radical, respectively. Total phenol contents of seed coat, seed, and part without seed coat were 12.7~21.0 mg%, 2.46~4.24 mg%, 0.94~1.16 mg% as tannic acid equivalent and those of Daekwang, Daesin, Bowon, Saedl, Kipung among the peanut varieties were comparatively high. In conclusion, Daekwang, Daesin, Kipung, and Saedl among the peanut varieties showed comparatively effective antioxidant activities.

징거미새우의 初期發生 및 飼育試驗(Ⅲ)

鄭泰永 釜山大學校 師範大學 1979 교사교육연구 Vol.6 No.2

1)Larvae of Macrobraeium nippoensis originated from Rak Dong River went through ten zoeal stages to the post larvae in 38 days approximately under the rearing laboratory conditions; pH 7~8, water temperature 23℃~26℃, chlonity 6.44~6.57‰while the other larvae of the same species originated from Kasumiga-ura Estury were required 18 days from 1st zoeal stage to 'post larval stage under similar conditions by the report Kwon. 2)The post laval stages were come after 10th zoeal stage ih this experiment but the post larvae in Kwon's experiment were come-after 9th zoeal stage. 3)Average body lengths of 1st zoeal stage were 1.58mm and average body lengths of post larvae were 4.91mm in this experiment. In Kwon's experiment, average body lengths oi 1st zoeal stage were 2.06mm and averge body lengths of post larvae were 5.31mm. In compavision, larvae of this experiment were smaller than that of Kwon's experiment through the whole stages. 4)Lots of difference occured in numbers of segments, setae, pulmouse setae of all appendages through whole stages and differentiation of periopods and pleopods were occured in some of different stages, between the two experiments. 5)Of the post larval stage, first tooth of rostrum was located on the middie portion of carpace in this experiment but the first tooth was located directly above the posterior margin of the orbit in Kwon's experiment, and flagellum length of 2nd Antenna was 1½ times of the scale jn this experiment while the flagellum length was a half of body length in Kwon's experiment. 6)In condition of this viewpoint, it seemed that Macrobracium nippoensis originated from Rak Dong River should be Ecotypes or Subspecies of the origin of Kasumiga-ura. Further-more,the two populations were isolated long geological times and not mixed. Further texonomical study compare with two populations of adult forms would be examined in future.