고지방과 콜레스테롤 식이로 유도된 고지혈증 흰쥐에서 노루궁뎅이버섯의 항고지혈증 효과

장형석,윤기남,Jang, Hyung Seok,Yoon, Ki Nam 대한임상검사과학회 2017 대한임상검사과학회지(KJCLS) Vol.49 No.3

This study was conducted to investigate the dietary supplementation of fruiting body of Hericium erinaceus (HE) mushroom on lipid profiles of serum and histological changes of the liver in rats with high fat and cholesterol diet. Five-week old female Sprague-Dawley albino rats were divided into three groups of 8 rats each: The normal control diet (NC group), high fat and cholesterol diet (HFC group), and HFC diet supplemented with 5% fruiting powder of Hericium erinaceus (HFC+HE group). In the HFC+HE group, serum total cholesterol, low density lipoprotein, and triglyceride concentrations were significantly reduced compared with the NC group. Body weight gain of those in the HFC+HE group were lower than those in the HFC group; whereas HFC+HE had no effect on the levels of plasma albumin, creatinine, blood urea nitrogen, uric acid, glucose, and total protein. The enzyme activities related to the liver function, such as aspartate aminotransferase (AST), alanine aminotransferase (ALT), and, alkaline phosphatase (ALP), were lower in the NC group than in the HFC group, but without significance. Feeding the mushroom increased the excretion of total lipid and cholesterol. A histopathological analysis showed that the those in the HFC group developed hepatic steatosis, whereas those in the HFC+HE group developed small fat droplet. In conclusion, these results suggest that 5% HE supplementation to HFC diet provided health benefits by acting on lowering atherogenic lipid profile in rats with high fat and cholesterol diet.

객담을 이용한 Mycobacteria의 검출과 중합효소 연쇄반응의 민감성 비교

장형석 ( Hyung Seok Jang ) 대한임상검사과학회 2015 대한임상검사과학회지(KJCLS) Vol.47 No.2

Although Mycobacterium tuberculosis complex strains remain responsible for the majority of diseases caused by mycobacterial infections worldwide, the increase in HIV (human immuno deficiency virus) infections has allowed for the emergence of other non-tuberculous mycobacteria as clinically significant pathogens. M. tuberculosis was detected by two-tube nested polymerase chain reaction (PCR) and non-tuberculous mycobacteria was detected by PCR-restriction fragment length polymorphism (RFLP) with Msp I. Result of niacin test is equal to result of two-tube nested PCR after culture for M. tuberculosis. In this study, acid fast bacilli stain (AFB. stain) >2+ case, Detection of Mycobacteria is similar to result before culture and after culture. AFB. stain <1+ case, result of mycobacteria is distinguished. Conclusionly, these results suggest that identification of mycobacteria must go side by side both culture and PCR for more fast and accuracy.

신령버섯 자실체 메탄올 추출물의 항산화, 항당뇨 및 Nitric Oxide의 저해 효과

윤기남 ( Ki Nam Yoon ),장형석 ( Hyung Seok Jang ),진가헌 ( Ga Heon Jin ) 대한임상검사과학회 2015 대한임상검사과학회지(KJCLS) Vol.47 No.4

Agaricus brasiliensis, belonging to the family Agaricaceae of Agaricales, Basidiomycota, has been used for edible and medicinal purposes. This study was initiated to evaluate the antioxidant, anti-diabetic, and nitric oxide inhibitory activities of fruiting bodies of A. brasiliensis extracted with methanol. The HPLC analysis of phenolic compounds from the mushroom extracts identified 10 phenolic compounds including gallic acid, procatechuic acid, chlorogenic acid, (-)-epicatechin, vanillin, rutin hydrate, naringin, quercetin, formononetin, and biochanin-A. The free radical scavenging activities of methanol extract were lower than that of positive control, BHT. The chelating effects of methanol extract were significantly higher than those of the positive control, BHT at the all concentrations tested. The methanol extract exhibited the lower reducing power activities compared with the positive control at the 0.5∼6.0 mg/mL concentration. The mushroom extract inhibited the α-glucosidase activity by 54.48% and 78.43% at the 1.0 and 2.0 mg/mL while acarbose, the positive control, inhibited the α-glucosidase activity by 51.77% and 81.81% at the same concentrations, respectively. Nitric oxide (NO) production in lipopolysaccahride (LPS) induced RAW 264.7 cells were inhibited by the methanol extracts in a concentration dependent manner. Therefore, it is concluded that fruiting bodies of A. brasiliensis contained natural antioxidant, anti-diabetic, and anti-inflammatory substances which can be useful for human health.

마른진흙버섯 자실체의 Xanthine Oxidase, Cholinesterase 및 염증 저해 효과

윤기남 ( Ki Nam Yoon ),장형석 ( Hyung Seok Jang ) 대한임상검사과학회 2018 대한임상검사과학회지(KJCLS) Vol.50 No.3

본 연구에서는 마른진흙버섯 자실체를 메탄올과 열수를 이용해 추출한 물질의 anti-xanthine oxidase, anti-cholinesterase 및 염증 저해 효과에 대한 연구를 수행하였다. 마른진흙버섯 자실체의 메탄올 추출물과 열수 추출물의 xanthine oxidase에 대한 저해효과는 양성대조군으로 사용한 allopurinol과 대등하게 높은 효과를 나타냈다. Acetylcholinesterase에 대한 메탄올 추출물의 1.0∼2.0 mg/mL 농도에서의 저해활성은 양성대조군인 galanthamine과 유사하게 높았지만 butyrylcholinesterase에 대한 메탄올과 열수 추출물의 저해활성은 양성대조군에 비해 실험에 사용한 모든 농도범위에서 유의하게 낮았다. PC-12 세포에 glutamate의 처리에 의해 유도된 독성은 40 mg/mL와 100 mg/mL 농도의 메탄올 추출물과 100 mg/mL 농도의 열수 추출물의 처리에 의해 크게 완화되어 PC-12 세포의 생존율이 유의하게 증가하는 것이 관찰되었다. 마른진흙버섯의 메탄올과 열수 추출물의 염증 저해 실험에서 RAW 264.7 대식세포에 메탄올 추출물을 2.0 mg/mL 농도로 처리하고 염증을 매개하는 LPS를 추가로 투여한 후 RAW 264.7 세포에 생성되는 NO를 측정한 결과, 양성대조군에 비해 3.37배 높은 저해효과를 나타냈고, 처리한 자실체 메탄올 추출물의 농도가 증가함에 따라 생성된 NO의 양이 현저하게 감소하는 경향을 나타내었다. 또한 기염제인 carrageenan에 의해 흰쥐 뒷발에 유도된 부종 저해 실험에서는 투여한 버섯 추출물의 농도가 증가함에 따라 흰쥐의 뒷발에 유도된 부종의 용적이 농도 의존적으로 감소하는 경향을 나타냈다. 따라서 마른진흙버섯 자실체에 함유된 물질은 acetylcholinesterase과 butyrylcholinesterase 등의 cholinesterase에 대한 저해작용과 glutamate에 의해 유도된 PC-12세포의 독성을 완화하고 또한 염증을 저해하는 효과를 나타내 기억력이 감퇴되는 초기 알츠하이머병과 염증을 완화하는 천연 소염제로의 이용이 가능할 것으로 사료된다. Phellinus gilvus is a medicinal mushroom used that has been used in folk medicine in Asian countries for centuries. The aim of this study was to investigate the anti-xanthine oxidase, anti-cholinesterase, and anti-inflammatory activities of methanol (ME) and hot water (HW) extracts prepared from fruiting bodies of Ph. gilvus. ME and HW had good anti-xanthine oxidase (XO) activities compared to allopurinol, an inhibitor of xanthine oxidase. ME showed comparable and slightly lower inhibitory activity against acetylcholinesterase (AChE) and butyrylcholinesterase (BChE), respectively, than galanthamine, a standard AChE and BChE inhibitor. ME also showed a protective effect against glutamate-induced cytotoxicity at 40 mg/mL and 100 mg/mL in PC-12 cells. ME (0.5∼2.0 mg/mL) significantly inhibited nitric oxide (NO) production in RAW 264.7 murine macrophage cells stimulated with lipopolysaccharide (LPS). Carrageenan-induced hind-paw edema in rats was significantly reduced 2∼6 hr after treatment with 50 mg/kg of ME, which was comparable to administration of 5 mg/kg of indomethacin, the positive control. These results demonstrate that ME and HW of Ph. gilvus fruiting bodies possess good anti-xanthine oxidase, anti-cholinesterase, and anti-inflammatory activities.

신체 부위별 표피에서 밀착연접 단백질 중 오클루딘의 발현도 연구

김지숙 ( Ji Sook Kim ),장형석 ( Hyung Seok Jang ) 대한임상검사과학회 2015 대한임상검사과학회지(KJCLS) Vol.47 No.4

Tight junctions (TJ) have recently been identified in the granular cell layer of the human epidermis, where they contribute to the normal adhesion between keratinocytes and to the physiologic barrier function of the epidermis. Among the TJ proteins in the epidermis, occludin is an important transmembrane protein, which is considered as a major component. The purpose of this study is to investigate whether regional variation exists in the expression of the tight junction protein occludin in normal human epidermis. Indirect immunofluorescence staining for occludin was performed with specimens taken from different areas of normal skin (4 from each of 7 different anatomical sites, including the scalp, face, posterior neck, upper arm, abdomen, lower back, and inner thigh). The degrees of the expression-intensity in each specimen were estimated with the reciprocals of positive end-point titer of occludin in an indirect immunofluorescence study. The highest degree expression-intensity of the TJ protein occludin among the different areas of normal epidermis was observed on the face and abdomen with a titer of 600 (p=0.001). The lowest intensity of expression of occludin was seen in the epidermis from the upper arm. Skin specimens from the scalp, neck, back, and leg demonstrated intermediate degrees of the expression in intensity. The expression of occludin in the skin samples obtained from different locations of the body showed a statistically significant variation. This suggests that there is a certain degree of regional variation in the expression-intensity of TJ protein ‘occludin’ in the human epidermis.

국내에서 수집된 마른진흙버섯 자실체의 항산화 및 항균 효과

윤기남 ( Ki-nam Yoon ),장형석 ( Hyung Seok Jang ) 대한임상검사과학회 2016 대한임상검사과학회지(KJCLS) Vol.48 No.4

This study was initiated to evaluate the antioxidant and antimicrobial activities of methanol extract (ME) and hot water extract (HWE) obtained from the fruiting bodies of medicinal mushroom, Phellinus gilvus. The free radical scavenging activity of ME from P. gilvus on 1,1-diphenyl-2-picrylhydrazyl (DPPH) were 93.65% at 2 mg/mL, which was comparable with the positive control, butylated hydroxytoluene (BHT, 96.97%) at the same concentration. The ferrous ion-chelating ability of ME and HWE was significantly higher than that of BHT at all concentration levels. The antimicrobial assay of ME was performed against six bacteria and one species of fungus. ME exhibited antibacterial activity against 5 out of 6 bacteria: Staphylococcus aureus, Streptococcus mutans, Bacillus subtilis, Escherichia coli, and Pseudomonas aeruginosa; whereas, ME did not show antimicrobial activity against gram-negative bacterium Vibrio vulnificus and fungal species Candida albicans. The minimum inhibitory concentration (MIC) of ME against 5 strains of bacteria, such as S. aureus, S. mutans, B. subtilis, E. coli, and P. aeruginosa, was 100, 100, 50, 100, 200 mg/mL, respectively. The results suggest that good antioxidant and microbial activities of P. gilvus fruiting bodies might be used for natural antioxidant and antimicrobial agents.

레일연마에 의한 철도차량 소음영향 검토

김종환(Jong Hwan Kim),장형석(Hyung Suk Jang),김재환(Jae Hwan Kim),임효석(Hyo Seok Lim),김병희(Byung Hee Kim) 대한기계학회 2018 대한기계학회 춘추학술대회 Vol.2018 No.5

작업환경측정 시료 채취기의 부착 위치에 따른 유기화합물의 농도 분포에 관한 연구

권영규 ( Yung Gyu Kwon ),원정일 ( Jung Il Won ),장형석 ( Hyung Seok Jang ),심상효 ( Sang Hyo Sim ) 한국산업보건학회 2015 한국산업보건학회지 Vol.25 No.3

Objectives: The purpose of this study is to investigate differences in concentration according to the position at the left or right shoulder within a 30 cm of radius of workers’ respirators and provide basic data for the establishment of an industrial health policy. Methods: Personal samples were collected from a total of 65 workers from 27 manufacturing firms in South Gyeongsang-do Province from November 5, 2011 to December 30, 2012 after classifying the laborers into left- and right-side groups. The organic compound samples were collected and analyzed in accordance with the NIOSH Manual of Analytical Methods (NMAM) 1501. Results: In terms of the concentration of organic compounds collected from both left and right shoulders at the position of workers’ respirators, isobutyl acetate was the highest with 145 ppm at the left shoulder, followed by ethyl acetate (133.5 ppm) and toluene (38.13 ppm). At the right shoulder, on the contrary, ethyl acetate (149.3 ppm) was the highest, followed by toluene (46.26 ppm), xylene (29.63ppm) and isopropyl alcohol (28.06 ppm). Overall, the right shoulder was higher than the left shoulder in terms of concentrations. Conclusions: For the measurement of the working environment, workers’ personal samples should be collected at the place closest to the respirator. In terms of the reduction of error, the attachment of two sample media is expected to reduce errors in exposure assessment.

저손실 재구성 분배기를 이용한 빔 성형 배열 안테나 설계

태현성(Hyun-Sung Tae),손왕익(Wang-Ik Son),장형석(Hyung-Seok Jang),오경섭(Kyoung-Sub Oh),유종원(Jong-Won Yu) 한국전자파학회 2012 한국전자파학회논문지 Vol.23 No.4

본 논문에서는 새로운 구조의 빔 성형 배열 안테나를 제안한다. 제안된 빔 성형 배열 안테나는 새로운 구조의 저손실 재구성 분배기 및 입체 배열을 통해, 빔 방향 및 빔 폭 제어 특성을 가진다. 여기서, 제안하는 재구성 분배기는 개별 안테나로의 재구성 가능한 RF 전력 분배 기능을 가지고 있기 때문에, 본 안테나에서의 핵심 기술이라 할 수 있다. 또한, 제안된 안테나의 연구 및 특성 입증을 위해, 본 논문에서는 입체형 빔 성형 배열 안테나를 재구성 분배기와 함께 구현하였으며, 실험적으로 RF 전력 분배 모드 제어를 통해 1:1부터 1:N까지 다양한 방사 모드를 가지고 있는 것을 확인하였다. In this paper, new beam-forming array antenna is proposed. The proposed beam-forming array antenna has control ability for beam-direction/width by employing low loss reconfigurable power divide and three-dimensional array structure. The reconfigurable power divider is key idea in the proposed antenna, because it has reconfigurable RF power distribution ability to each antenna. And, for research and verification of the proposed antenna, 3-dimensional beamforming array antenna is implemented, and the experimental results show that the proposed antenna has various radiation modes from 1:1 to 1:N by adjusting RF power distribution.

균열저감 콘크리트의 제조사별 수축특성 평가

이지환(Lee, Ji-Hwan),박희곤(Park, Hee-Gon),박기홍(Park, Gi-Hong),진정언(Jin, Jeong-Eon),장형석(Jang, Hyung-Seok),김준년(Kim, Joon-Yeoun) 한국콘크리트학회 2017 한국콘크리트학회 학술대회 논문집 Vol.29 No.1