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흰쥐 이하선 선세포에서 Potassium Release와 관련된 Calcium 동원계
서정택,김순이,이승일 대한구강생물학회 1992 International Journal of Oral Biology Vol.16 No.1
On stimulating receptors(muscarinic, substance P) in plasma membrane of the salivary gland, the elevation of cytosolic cacium(Ca^2+) evokes the salivary secretion. There are two basic mechanisms fo Ca^2+ mobilization by which the cell increase its cytosolic free calcium : 1) via Ca^2+ release triggered by inositol trisphosphate (IP_3), and 2) via entry of extracellular Ca^2+. In addition, there are several possibilities by which intracellular Ca^2+ concentration may be modlulated by the IP_3-insensitive Ca^2+ pool (IICP) and intracellular Ca^2+ mobilization might be sophisticately intercommunicated with types of agonists and the interaction among agonists. This study was aimed at characterizing the Ca^2+ mobilization system of rat parotid glands in resonse to carbachol (CCh) and substance P (Sub-P). The minced tissue of rat parotid acini was placed in the perifusion chamber with the K^+-sensitive electrode(Orion Research), followed by perfusing the Krebs-Ringer bicarbonate solution. In order to stimulate the parotid acini, CCh and Sub-P were perfused with KRB solution. Also the K^+-release from parotid acini was measured and interpreted in terms of the intracellular Ca^2+ concentration. In the absence of Ca^2+ the parotid acini was repeatedly stimulated by CCh. As a result, CCh-induced K^+-release was dramatically reduced. However, the addition of Ca^2+ caused an increase in the CCh-induced K^+release. On the other hand, staurosporine(10^-6M), an inhibitor of PKC, did not affect the CCh-induced K^+release. A dose of 10 mM caffeine given to parotid acini increased the K^+release in both cases of Ca^2+, and Ca^2+ free solution. But the level of K^+release was sustained higher in the presence of Ca^2+. In addition, parotid acini were stimulated by the CCh under the condition of caffeine pretreatment. Consequently, CCh-induced K^+release was remarkably inhibited in the presence or absence of Ca^2+, while Sub-P-induced K^+release was not changed by the caffeine pretreatment. Nevertheless, if CCh or Sub-P was administrated with caffeine at a time. Caffeine did not blunt the CCh-induced K^+release. Furthermore, the removal of caffeine in the sustained phase resulted in the successive increase of K^+release by the CCh, while not in the case of Sub-P. Taken all together, CCh-and Sub-P-induced K^+release seem to be caused by Ca^2+ entry and the cytosolic Ca^2+ released from internal Ca^2+ store. However, the regulation of Ca^2+ mobilization of Sub-P might be different from that of CCh. In particular, the process of Ca^2+ entry may be closely associated with the filling state of internal Ca^2+ store. However, the interrelation between Ca^2+ entry and internal Ca^2+ store in terms of Ca^2+ mobilization, should be remained to be solved.
남궁문,이병주,손영국,이정택 圓光大學校 環境建設硏究所 1999 環境建設論文集 Vol.8 No.-
In this paper, we surveyed to population of Iksan for that what is the major obstacle factor in policy which activate to use bicycle before introducing the green transportation policy. Also, for understanding the degree of affection the qualitative factor as road images for using the bicycle, we firstly analyze to the road images using the Principal Analysis and then construct the Choce Model using the LISREL(Linear Structural Relationships). As the result of scheme, the major factor is a degree of danger about crashing between two road users on roads. Therefore, before introducing the green transportation policy for activating to use bicycle as a greed mode, if the authorities make a alternative for protect bicycle user from so many dangerous factor, introduction of the green transportation policy using bicycle will be built successfully.