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이삼선,김재원,지차호,Lee, Sam-Sun,Kim, Jae-Won,Jee, Cha-Ho 대한수의학회 2002 大韓獸醫學會誌 Vol.42 No.2
Fowl red mite, Dermanyssus gallinae, is the most important ectoparasite affecting egg layers worldwide. More than 35 compounds have been used for fowl red mite control. Although some of them are efficient, several compounds are unsuitable in terms of food safety and environmental problems. Some compounds are efficient in theory but inadequate in practice. It is also expensive in material and labor to control effectively. Effective doses are very close to toxic doses and repeated treatment is required. Repeated, long term treatment of compounds on fowl red mite populations, may cause heritable resistance against the mites. In this study, antigenicity of fowl red mite and house dust mite, Dermatophagoides pteronyssinus, were identified by SDS-PAGE, silver staining, Western blotting and ELISA to investigate immune effects against fowl red mite using somatic antigens of fowl red mite and house dust mite. By SDS-PAGE, silver staining and Western blotting, several common antigens (110, 60, 56, 49, 46 kDa) of both fowl red mite and house dust mite were recognized. To identify immune effect of somatic antigens of fowl red mite and house dust mite, sixty white leghorn broilers(1 week old) were used. Among sixty white leghorn broilers, twenty were immunized with fowl red mite somatic antigens(Group I), twenty immunized with house dust mite antigens(Group II), and twenty were control group without antigen(Group III), respectively. After immunization, it was identified that antibody titers were increased both in group and II. Then all groups were challenged with fowl red mites. After 2 months, measurements of body weights, packed cell volume(PCV), ELISA OD values and numbers of mites were significant(p<0.05). These results suggest that fowl red mite and house dust mite, which are easy to collect and maintain, can be good vaccine candidates against fowl red mite in chicken.
마삼선(Sam Sun Ma),윤용범(Yong Beum Yoon),안남성(Nam Sung Ahn),이재걸(Jae Gul Lee),김현실(Hyun Sil Kim) 대한전기학회 2006 대한전기학회 학술대회 논문집 Vol.2006 No.7
계통운영 보조서비스는 전력계통의 안정성 확보, 전기품질 유지 및 전력거래 활성화를 위한 필수적인 서비스이다. 계통운영 보조서비스의 비용요소는 연료비, 운영유지비, 자본비용, 기회비용으로 구분된다. 이의 적정비용규모를 산정하여 보조서비스 제공자에게 보상함으로써 전력의 공급안정성 확보에 크게 기여할 수 있게 된다. 여기서는 국내 진력시장에서 연료비와 운영유지비 측면에서 적정비용규모를 산정하고 현재 운용중인 시장규칙과 비교하여 개선방안을 제시한다.
이삼선(Sam-Sun Lee) 대한치과의사협회 2014 대한치과의사협회지 Vol.52 No.3
The quality assurance program of a panoramic radiography is been discussed in two ways: phantom image tests and clinical image quality assurance tests. Methods and standards of each of the test are presented, which efforts guarantee a proper quality of image, improve standard of dental care and decrease radiation exposure.
모델변환을 이용한 시간지연 선형시스템의 지연종속 $H_{\infty}$ 제어
마삼선,이희송,김진훈,Ma, Sam-Sun,Lee, Hee-Song,Kim, Jin-Hoon 대한전기학회 2001 전기학회논문지 D Vol.50 No.9
This paper deals with the design of delay-dependent $H_{\infty}$ control for time-delayed linear systems. We propose the two different model transformations to handle efficiently the time delay and derive conditions linear matrix inequalities (LMI's) of which are dependent on the size of time-delay and its time-derivative. The $H_{\infty}$ controller is obtained by checking the feasibility of these LMI's. Finally, we show the usefulness and applicability of our results by an example.
승삼선 ( Sam Sun Seung ),이승철 ( Seung Chul Lee ) 대한설비관리학회 2010 대한설비관리학회지 Vol.15 No.2
It is important to evaluate thermal environmental characteristics and the amenities in a large space such as a building with a performance hall. Because there are sometimes occurred inefficient HVAC system operation and excessive energy consumptions. Especially in a thermal environmental view, people feel thermal displeasure because of temperature differences between vertical and horizontal directions. We have performed thermal environmental characteristics analysis for N Korean performance arts center by using CFD techniques. Consequently we confirmed that supply flow balancing after T.A.B decreased thermal stratification locally and increased people`s thermal comfort. So it is necessary to carry out T.A.B for HVAC system in a large space. And it can be used as a fundamental design data for large space design.
방사선의 선량변화가 수종의 정상세포와 종양세포주의 세포활성도와 apoptosis 유발에 미치는 영향
박인우,이삼선,허민석,최순철,Park In-Woo,Lee Sam-Sun,Heo Min-Suk,Choi Soon-Chul 대한영상치의학회 1999 Imaging Science in Dentistry Vol.29 No.2
Purpose : The study was aimed to detect the differences in the cell viability and the apoptosis induction after irradiation on normal and tumorigenic cells. Materials and Methods : The study. that was generated for two human normal cells(RHEK, HGF-l) and two human tumor cells(KB. HT-1080). was tested using MTT assay at 1 day and 3 day after irradiation and TUNEL assay under confocal laser scanning microscope at 1 day after irradiation. Single irradiation of 0.5. 1, 2. 4. and 8Gy were applied to the cells. The two fractions of 1. 2. 4. and 8Gy were separated with a 4-hour time interval. The irradiation was done with 5.38Gy/min dose rate using Cs-137 irradiator at room temperature. Results and Conclusions : 1. In 3-day group. the cell viability of HGF-1 cell was significantly decreased at 2. 4 and 8Gy irradiation, the cell viability of KB cell was significantly decreased at 8Gy irradiation and the cell viability of HT-I080 cell was significantly decreased at 4 and 8Gy irradiation. 2. There was significant difference between RHEK and KB cell line in the cell viability of 3-day group at 8Gy irradiation. There was significant difference between RHEK and HGF-1 cell line in the cell viability of 3-day group at 4 and 8Gy irradiation. 3. There was a significantly decreased cell viability in 3-day group than those in 1-day group at 2. 4 and 8Gy on HGF-1 cell. at 4 and 8Gy on HT-I080 cell. at 8Gy on KB cell. 4. We could detect DNA fragmented cells only on KB cell. Number of apoptotic cells of KB cell was significantly increased at 4 and 8Gy irradiation. However, there was no correlation between cell viability and apoptosis. 5. On all 4 cell lines, there were no differences between single and split irradiation method in cell viability and apoptosis.
인체의 암세포주와 치은섬유모세포주에서 방사선조사가 apoptosis 유발에 미치는 영향에 관한 연구
박무순,이삼선,최순철,박태원,유동수,Park Moo-Soon,Lee Sam-Sun,Choi Soon-Chul,Park Tae-Won,You Dong-Soo 대한영상치의학회 1998 Imaging Science in Dentistry Vol.28 No.1
The radiation-induced apoptosis was studied for two human cancer cell lines (KB cells, RPMI 2650 cells) and the human gingival fibroblast cell line (HGF-1 cells). The single irradiation of 2, 10, 20Gy was done with 241.5 cGylmin dose rate using the /sup 137/Cs MK cell irradiator. The cells were stained with propidium iodide and examined under the fluoro-microscope and assayed with the flow cytometry a day after irradiation. Also, the LDH assay was done to determine the amount of necrotic cells. The obtained results were as follows: 1. On the fluoro-microscope, many fragmented nuclei were detected in the KB, RPMI 2650, and HGF-1 cells after irradiation. 2. On the DNA content histogram obtained from the flow cytometry, the percentages of the pre-Gl peak of the control and 2, 10 and 20Gy irradiation group were 4.5, 55.0, 52.3, and 66.6% on KB cells, 2.7, 3.3, 31.8, and 32.6% on RPMI 2650 cells and 2.8, 21.8, 30.4, and 40.2% on HGF-1 cells respectively. 3. The number of Gl-stage cells was abruptly decreased after 2Gy irradiation on KB cells and 10Gy irradiation on RPMI 2650 cells, But there was a slight decrease without regard to irradiation dose on HGF-1 cells. 4. There was no significantly different absorbance in extracellular LDH assay along the experimental cell lines.
버스전용 지하주차장의 환기 특성에 관한 수치해석적 연구
이승철(Lee, Seung-Chul),승삼선(Seung, Sam-Sun) 한국산학기술학회 2007 한국산학기술학회논문지 Vol.8 No.2
본 연구에서는 전산유체역학 기법을 도입하여 17대 버스가 주차된 지하주차장의 환기특성을 분석하였다. 그 결과 지하주차장내 버스 4대가 운행할 때 CO와 NO의 평균농도는 각각 1.3과 0.3 ppm으로 나타났다. 또한 15대 버스가 공회전할 때 지하주차장내의 CO와 NO<sub>x</sub>의 평균농도는 2.9와 0.8 ppm으로 각각 나타났다. 따라서 두 가지 경우의 결과가 허용기준을 충족하여 지하주차장에 설치될 환기설비의 용량 및 위치는 적정한 것으로 판단된다. In this paper, the ventilation characteristics of an underground parking lot with 17 buses has been investigated for two cases by computational fluid dynamics. It has been found that the average concentration of CO and NO<sub>x</sub> gas in the parking lot were 1.3 and 0.3 ppm respectively, when four buses operated. When the fifteen vehicles stopped in the parking lot, the average concentration of CO and NO<sub>x</sub> gas was 2.9 and 0.8 ppm respectively. The average concentration of two pollutants(CO, NO<sub>x</sub>) are lower than the standard values. Therefore the facilities and its location is proper to ventilation.