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NANB 간질환 환자에서 2세대 효소 면역 검사법과 중합효소 연쇄 반응법을 이용한 C형 간염바이러스 감염의 진단
이복근(Bock Gyeon Lee),권기호(Ki Ho Kwon),박양훈(Yang Hun Park),김한성(Han Sung Kim),정찬수(Chan Su Jeong),제영성(Young Sung Jae),이준상(Joon Sang Lee),유방현(Bang Hyun Liu),박영홍(Young Hong Park),이기영(Kee Young Lee) 대한소화기학회 1994 대한소화기학회지 Vol.26 No.5
N/A The first-generation enzyme linked imrnumosorbent assay(ELISA) method to detect hepati- tis C virus(HCV) has been used with relatively good sensitivity in the diagnosis of chronic non A non B hepatitis infecton, but its sensitivity in cases of acute hepatitis remains poor. The secondeneration ELISA which uses recombinant proteins and synthetic peptides from both structural and nonstructural regions increases the sensitivity in diagnosing HCV infection, and diminishes the number of false-positive reactions. The polymerase chain reaction(PCR) has been used to detect infectivity of anti HCV positive patients more accurately, to evaluate ver- tical transmission, and to monitor the response to antiviral ther~apy for chronic HCV infection. Serum samples from 35 patients with non A non B type liver disease were tested by the secondeneration ELISA and the PCR to evaluate the roles of hepatitis C viral infections in NANB patients. HCV RNA was detected by single polymerase c~t~ain reaction technique using primers deduced from the 5noncoding region of HCV genome. Among the 35 patients, 27 pa- tients were positive for anti HCV (Group A), while 8 were negative for anti HCV(Group B). Among anti HCV positive patients, 11% and 33% o~f patients were related to the transfu- sion and acupuncture, respectively. We could detect HCV RNA in the sera from 27 patients (100%) in the group A and 4 patients(50%) in the group B. We conclude that the combination methods of secondeneration ELISA plus PCR reduce the chance of false diagnosis of HCV infection compared to the first generation ELISA and the PCR can be used to diagnose HCV infection in anti HCV negative hepatitis patients.(Ko- rean J Gastroenterol 1994; 26; 825 832)