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반추가축영양 : 생균제 개발을 위한 새로운 효모균의 선별
신형태(H . T . Shin),금동혁(D . H . Keum),이향우(H . W . Lee),이동권(D . K . Rhee),황보식(B . S . Hwang),이재홍(J . H . Lee) 한국축산학회 2001 한국축산학회지 Vol.43 No.5
A thermotolerant strain of yeasts was isolated from cane molasses at 39℃ from the view of commercial potential of direct-fed microbials (DFMs), and tested for its tolerance to acid in the pH range 1.0∼7.0, bile salts and a mixture of volatile fatty acids (acetic acid: propionic acid: butyric acid= 6.5:2.0:1.5). For comparative purposes, various Saccharomyces cerevisiae strains were also used. The isolated strain DY01 grew very well at up to pH 2.0 and was resistant to high concentrations (0.6%∼0.9%, w/v) of bile salts. Among the strains tested, DY01 showed least inhibition (i.e. 40∼45% inhibition) with 0.25% (v/v) addition of a mixture of volatile fatty acids at 39℃. It appears that this strain, identified as yeast lssatchenkia orientalis. may be used as a potential candidate for a DFM.