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- 저자 : 이근배 ( Seung Young Lee (검색결과 4 건)
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체중 부하 후 전족부 배열의 방사선적 변화: 무지 외반각 및 중족골간 각과 내측 종자골의 위치
노성만,이근배,최진,천승영,허창익,Rowe, Sung-Man,Lee, Keun-Bae,Choi, Jin,Cheon, Seung-Young,Hur, Chang-Ich 대한족부족관절학회 2005 대한족부족관절학회지 Vol.9 No.1
Purpose: To determine the radiographic changes in forefoot geometry with weight-bearing. Materials and Methods: The forefoot radiographs of 100 normal Korean adults, 50 male and 50 female volunteers, were evaluated both in nonweight-bearing and weight-bearing. The mean age was 27 years with range of 21-39 years. Those with normal feet were selected from volunteers having no history of foot problems or other musculoskeletal diseases. Results: The changes of measured angle between phalanges and metatarsals with weight-bearing were as follows; Hallux valgus angle was noted to increase in 20% of the feet, decrease in 59%, and remained unchange in 21%. Intermetatarsal angle $1{\sim}2$ was noted to increase in 76% of the feet, decrease in 3%, and remained unchange in 21%. Intermetatarsal angle $1{\sim}5$ was noted to increase in 95% and remained unchange in 5%. Shift in medial sesamoid on weight-bearing was also not consistent. Lateral shift was noted in 27%, no shift in 66%, medial shift in 7%. Conclusion: The generalized concept that the angles between bones and shift of medial sesamoid in the forefoot will change consistently with weightbearing was not found.
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Histone에 관한 연구(XIV) Paramphistomum cervi의 Histone
이승영,이근배,Lee, Seung-Young,Lee, Keun-Bai 생화학분자생물학회 1978 한국생화학회지 Vol.11 No.3
Paramphistomum cervi의 총 histone의 분획을 관찰하여 다음과 같은 결과를 얻었다. Histone을 Johns 및 Phillips and Johns의 방법으로 분리 추출하였다. 각 분획은 polyacrylamide disc gel 전기이동법에 의하여 정제하였다. 총 histone의 수율은 조직 100g당 569mg이었으며 이 값은 포유동물에 비하면 좀 큰 값이다. DNA는 206mg이었다. 따라서 DNA : histone 의 비는 1 : 0.37이다. Paramphistomum cervi의 5개의 분획 즉 H1, H2a, H2b, H3 및 H4의 함유량의 비는 13.2%, 3.7%, 26.9%, 51.5% 및 4.7% 이며 포유동물에 비하면 현저한 차이를 보여주고 있다. Paramphistomum cervi의 histone의 각 분획들의 amino산 조성을 보면 각 histone이 포유동물 및 고동식물의 값과 동일하지는 않으나 비슷한 값을 보여주고 있다. 현재까지 기생충의 histone에 관한 연구는 찾아볼 수 없으며 저자의 연구로써 Paramphistomum cervi는 진핵세포를 가진 고등동물 및 식물속에 들어있는 5개의 histone 분획과 같은 histone 분획들을 가지고 있음이 밝혀졌으며, 또 그 histone 단백질이 강한 보수성을 지니고 있음이 확인되었다. The five major fractions of histone in Paramphistomum cervi (P. cervi) were isolated and purified according to the method of Phillips and Johns. This is the first of such isolation from parasite tissues. The histone fractions were identified by the acrylamide disc gel electrophoresis and by amino acid analysis. Amino acid compositions of purified histione fractions indicated that the histones were homologous, if not identical, to the H1, H2a, H2b, H3 and H4 histones of calf thymus. The yield of whole histone recovered was 569mg per 100g of weight of the parasite. This value was slightly larger than that of mammalian tissues. The yield of DNA was 206mg per 100g of wet weight of the parasite. Consequently, the ratio of DNA to histone was 0.37. The relative amounts of 5 histone fractions from P. cervi., i.e., H1, H2a, H2b, H3 and H4 were 13.2%, 3.7%a, 26.9%, 51.5% and 4.7%, respectively. This finding of relatively low proportion of histones H2a, and H4 and high proportion of histone H3 in P. cervi. appeared to be due to the species specificity. The electrophoretic behaviors of each histone fractions were almost similar to those of histones from calf thymus. The apparent difference in the ratio of DNA to histone between P. cervi higher organisms was observed, showing the ratio of the parasite: had approximately one third as much the ratio of the animal. The evidence was presented that P. cervi. contained five major histore fractions which were homologous to corresponding 5 fractions of higher eukaryotes, and this study confirmed earlier observations strongly on the conserved primary sequence of histone proteins in nature.
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Histone 에 관한 연구 ( ⅩⅣ ) Paramphistomum cervi 의 Histone
이승영,이근배 ( Seung Young Lee,Keun Bai Lee ) 생화학분자생물학회 1978 BMB Reports Vol.11 No.3
The five major fractions of histone in Paramphistomum cervi (P. cervi) were isolated and purified according to the method of Phillips ar.d Johns. This is the frist of such isolation from parasite tissues. The histone fractions were identified by the acrylamide disc gel electrophoresis and by amino acid analysis. Amino acid compositions of purified histione fractions indicated that the histones were homologous, if not identical, to the H1, H2a, H2b, H3 and H4 histones of calf thymus. The yield of whole histone recovered was 569mg per 100g of weight of the parasite. This value was slightly larger than that of mammalian tissues. The yield of DNA was 206mg per 100g of wet weight of the parasite. Consequently, the ratio of DNA to histone was 0.37. The relative amounts of 5 histone fractions from P. cervi., i.e., H1, H2a, H2b, H3 and H4 were 13.2%, 3.7%a, 26.9%, 51.5% and 4.7%, respectively. This finding of relatively low proportion of histones H2a, and H4 and high proportion of histone H3 in P. cervi. appeared to be due to the species specificity. The electrophoretic behaviors of each histone fractions were almost similar to those of histones from calf thymus. The apparent difference in the ratio of DNA to histone between P. cervi higher organisms was observed, showing the ratio of the parasite: had approximately one third as much the ratio of the animal. The evidence was presented that P. cervi. contained five major histore fractions which were homologous to corresponding 5 fractions of higher eukaryotes, and this study confirmed earlier observations strongly on the conserved primary sequence of histone proteins in nature.
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