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      • 家蠶에서 分離된 새로운 微胞子蟲 K79의 病理學籍 硏究 : Ⅱ .微胞子蟲의 病原成 및 發育經過 Ⅱ.Pathogenicity and Developmental Progress of the Microsporidia

        尹在洙,林種聲 한국잠사학회 1985 한국잠사곤충학회지 Vol.27 No.1

        In order to clarify the taxanomic position of a new microsporidia K79 which was isolated from the silkworm larvae, Bombyx mori L. in Korea in 1979, the following several experiments such as estimation of pathogenicity in diferent instar, histopathological examination under light and electronic microscope and examination of fine structures of the spores were carried out and their results obtained are as follows. In the test of pathogenicity by oral inoculation, the new microsporidia K79 was lower than Nosema bombycis and the susceptibility of the new microsporidia to silkworm was getting lower as the silkworm larvae grew. The lesion of Silkworms' tissue which was infected with the new microsporidia K79 was found in the epithelial cells of trachea, fat body and silk gland cells. The developmental process of the new microsporidia K79 in vivo could be divided into the following five stages: sporoplasm, schizont, sporont, sporoblast, and spore. This process was just the same as that of N. bombycis, but its development was slower than that of N. bombycis. Several differences in the fine structure of the spore under electron microscope, which could be important keys for the classification of microsporidia, were obtained. Anchoring disk and polaroplast lamella of the new microsporidian spore were disclosed to be different from those of N. bombycis. An average number of polar filament coils of the new microsporidian spore was 16 at an angle of 75°. On the basis of various keys for the classification of microsporidia, the results obtained from various experiments proved that the newly isolated microsporidia should be classified into the Genus, "Nosema", but its further classification for species should be conducted in the future. Therefore, it may be reasonable that the new microsporidia is temperally classified as Nosema sp. K79 considering the fact that it was discovered in Korea in 1979.

      • SCOPUSKCI등재

        눈꽃동충하초균의 누에 감염이 고치에 미치는 영향

        윤재수 한국곤충학회 2001 Entomological Research Vol.31 No.2

        눈꽃동충하초균의 감염이 고치에 미치는 영향을 알아보기 위하여 5령 누에에 균을 접종하고 실험을 수행하였다. 저 농도 눈꽃동충하초균의 침입을 받은 누에는 건강한 누에처럼 생존하여도 경과일수의 지연, 체중의 감소를 가져 왔고, 견질면으로 보아도 고치 무게, 고치층 무게, 번데기 무게 등의 감소로 견질이 저하되었다. Cordyceps, Paecilomycesjaponica, was inoculated against silkworm, Bombyx mori in the 5th instals to know effect of cordyceps on the cocoon quality. Feeding period of larvae in 5th instals was more longer than normal larvae. Larval body weight, cocoon weight, cocoon layer weight, and pupal weight were reduced 21.56%, 13.64%, 15.18% and 11.74% respectively. The cocoon quality of silkworms which inoculated with cordyceps was inferior than cocoon quality of normal silkworms.

      • KCI등재
      • KCI등재

        Effect of Cordyceps, Paecillomyces sp., Cordyceps pruinosa and Paecillomyces japonica, on the Development of Domestic Silkworm, Bombyx mori

        윤재수 한국곤충학회 2005 Entomological Research Vol.35 No.4

        This study was carried out to investigate pathogenicity of cordyceps on the silkworm larva, Bombyx mori. For experiment, Cordyceps, Pacillomyces sp. strain collected from Chilgab mountain area in Chungnam Province, Cordyceps pruinosa collected from Chiak mountain area in Gangwon Province and Pacillomyces japonica collected from dead silkworm larva at the rearing room in Sangju National University were used. Spores of cordyceps were propagated on sterile PDA (potato dextrose agar 39 g, water 1,000 mL) at 25.C for 21 days. Spores of cordyceps isolated from media were inoculated on integuments of 3rd instar newly exuviated silkworm for pathogenicity on the silkworm larvae. The cordyceps used in this study was highly infectious to the silkworms. Virulence of cordyceps was different depending on species. Pacillomyces japonica was the most efficacious with 70% mortality on silkworm larvae. Cordyceps had adverse effect on the overall rearing from larval period to mounting of matured silkworm larvae, cocoon making, pupation and moth emergence. The result suggests that cordyceps used in this study may be useful for the tracking of biocontrol.

      • KCI등재

        Studies on Entomopathogenic Fungi Isolated from Dead Pine Caterpillars, Dendrolimus spectabilis

        윤재수 한국곤충학회 2003 Entomological Research Vol.33 No.4

        Entomopathogenic fungi isolated from pine caterpillars, as biological control agents were evaluated against the 5th instar larvae of silkworms, bombyx mori. The body weights of silkworm larvae were reduced by entomopathogenic fungi. Virulence of the spores isolated from the insects was more stronger than that of the spores isolated from media. LD50 value of the pathogen was 6,464×103 spores/ml. Entomopathogenic fungi, Beauveria, isolated from dead pine caterpillars was possible agents for use in microbiological control.

      • 나비목 해충에 대한 곤충병원성곰팡이, Beauveria bassiana와 Metarhizium anisopliae의 병원성

        윤재수,김형환,김도완,이상명,김동수,이동운,Yun Jae-Su,Kim Hyeong-Hwan,Kim Do-Wan,Lee Sang Myeong,Kim Dong-Soo,Lee Dong-Woon 한국잔디학회 2004 한국잔디학회지 Vol.18 No.4

        경북지방에서 분리한 곤충병원성곰팡이 Beauveria bassiana와 Metarhizium anisopliae를 이용하여 나비목 해충인 거세미나방 (Agrotis segetum), 배추흰나비 (Artogeia rapae), 도둑나방 (Mamestra brassicae), 배추좀나방(Plutella xylostella), 파밤나방 (Spodoptera exigua), 담배거세미나방 (S. litura)의 생물적 방제 가능성을 알아보기 위하여 실험한 결과, 거세미나방 2$\cdot$3령충에 대하여 B. bassiana와 M. anisopliae의 반수치사농도 ($LC_50$)는 접종7일 후에 각각 $4.3\times10^6\;conidia/m\ell$와 $2.4\times10^5\;conidia/m\ell$였다. 배추흰나비 2$\cdot$3령충에 대한 B. bassiana와 M. anisopliae의 $LC_50$는 접종 7일 후에 각각 $1.2\times10^7\;conidia/m\ell$와 $5.2\times10^6\;conidia/m\ell$였다. 도둑나방 2$\cdot$3령충에 대한 B. bassiana와 M. anisopliae의 $LC_50$는 접종 7일 후에 각각 $1.5\times10^6\;conidia/m\ell$와 $9.7\times10^5\;conidia/m\ell$였고, 배추좀나방 2$\cdot$3령충에 대한 B. bassiana와 M. anisopliae의 $LC_50$는 각각 $3.0\times10^6\;conidia/m\ell$와 $1.4\times10^6\;conidia/m\ell$였다. 파밤나방 2$\cdot$3령충에 대한 B. bassiana와 M. anisopliae의 $LC_50$는 접종 7일 후에 각각 $6.3\times10^6\;conidia/m\ell$와 $2.6\times10^6\;conidia/m\ell$였고, 담배거세미나방 2$\cdot$3령충에 대한 B. bassiana와 M. anisopliae의 $LC_50$는 각각 $1.6\times10^7\;conidia/m\ell$와 $3.4\times10^6\;conidia/m\ell$였다. Biological control of lepidopterous insect pests, Agrotis segetum, Artogeia rapae, Mamestra brassicae, Plutella xylostella, Spodoptera exigua, and S. litura with entomopathogenic fungi, Beauveria bassiana and Metarhizium anisopliae isolated from Gyeongbuk province were investigated. Mean lethal concentration ($LC_50$) values of B. bassiana and M. anisopliae against $2\cdot3rd$ instar of A. segetum larvae were $1.2\times10^7\;conidia/m\ell$ and $5.2\times10^6\;conidia/m\ell$, respectively. $LC_50$ values of B. bassiana and M. anisopliae against $2\cdot3rd$ instar of A, rapae larvae were $1.2\times10^7\;conidia/m\ell$ and $5.2\times10^6\;conidia/m\ell$, respectively. $LC_50$ values of B. bassiana and M. anisopliae against $2\cdot3rd$ instar of M. brassicae and P. xylostella, larvae were $1.5\times10^6\;conidia/m\ell$, $9.7\times10^5\;conidia/m\ell$, $3.0\times10^6\;conidia/m\ell$ and $1.4\times10^6\;conidia/m\ell$, respectively. $LC_50$ values of B. bassiana and M. anisopliae against $2\cdot3rd$ instar of S. exigua, and S. litura larvae were $6.3\times10^6 \;conidia/m\ell$, $2.6\times10^6\;conidia/m\ell$, $1.6\times10^7\;conidia/m\ell$ and $3.4\times10^6\;conidia/m\ell$ respectively.

      • KCI등재

        緣坐와 連坐制攷

        윤재수 동아대학교 석당학술원 1983 石堂論叢 Vol.8 No.-

        1. 緣生與連坐 犯罪因行爲人具備犯罪構成要件而成立, 此爲現代刑法之適例, 但明朝及朝鮮王朝律則因政策上理由, 本人縱無犯罪, 祗因其與犯罪人的正犯(主犯)有一定身分關係, 卽予處罰, 此乃緣坐與連坐。緣坐因本人與犯罪人有家族關係或一定範圍內之親屬關係而坐罪。卽所謂旅刑及三旅刑。蓋爲收威혁之效而設。親屬之緣坐, 係由於親屬一體的觀念, 令其負緣帶責任。緣坐, 乃逕對事實加以處罰。卽緣坐人言, 其受處罰之。連坐, 乃本人與犯罪人係同僚或上下官司之闢係而坐罪。連坐, 通常因同僚或上下官司關係之故, 令其負連帶責任。1)緣坐:關於犯罪的刑事上之責任, 係於行爲者本人一身上爲止, 可是在於特別的犯罪事例上, 因爲與犯罪人的正犯有某一種身分上之關係, 故産生了使負刑事上緣帶責任的緣坐, 與人民組成?伍, 關於?伍內的犯罪, 要盡告發之義務, 如有違犯時, ?伍科與刑罰的緣坐。其稱謂緣坐制。2)連坐:關於某一官吏所犯之公罪, 僅是以其他同僚官吏在文案上連署的行爲, 就能科與刑罰的連坐, 其稱謂連坐制。2. 緣坐與連坐之區別 綠坐與連坐, 雖然同是因他人之行爲緣(連)累于犯罪而被處罰, 他在行爲方面上或犯罪構成要件方面上, 타的性質完全不同。1) 緣坐之性質:犯罪行爲人的家族及親屬之身分關係, 以及?伍關係, 雖然不會犯法可是在刑事上被緣累, 要負責任, 其稱爲緣坐。2) 連坐之性質:完成文案行爲人之同僚官吏與上下官司, 在文案僅是因做了連署的行爲而連累至刑事上之責任, 其稱爲連坐。緣坐與連坐, 明顯的不同之要因, 卽緣坐是緣關親屬或?伍之關係, 再連坐是連關同僚或上下官: 之闢係而區別。

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