Therapeutic Effect of IL1β Priming Tonsil Derived-Mesenchymal Stem Cells in Osteoporosis

유민주,Cho Sungkuk,Shin Sunhye,Kim Jung-Mi,박현경,Cho Sungyoo,Hwang Yu Kyeong,박대휘 한국조직공학과 재생의학회 2021 조직공학과 재생의학 Vol.18 No.5

Background: Stem cell therapies can be a new therapeutic strategy that may rebalance anabolic and anti-resorptive effects in osteoporosis patients. Tonsil-derived mesenchymal stem cells (TMSCs) can be an alternative therapeutic source for chronic degenerative diseases including osteoporosis. MSCs acquire immune regulatory function under the inflammatory cytokines. Since interleukin (IL) 1β is known to be one of inflammatory cytokines involved in osteoporosis progression, treatment of IL1β with TMSCs may enhance immunomodulatory function and therapeutic effects of TMSCs in osteoporosis. Methods: For IL1β priming, TMSCs were cultured in the presence of the medium containing IL1β for 1 day. Characteristics of IL1β priming TMSCs such as multipotent differentiation properties, anti-inflammatory potential, and suppression of osteoclast differentiation were assessed in vitro. For in vivo efficacy study, IL1β priming TMSCs were intravenously infused twice with ovariectomized (OVX) osteoporosis mouse model, and blood serum and bone parameters from micro computed tomography images were analyzed. Results: IL1β priming TMSCs had an enhanced osteogenic differentiation and secreted factors that regulate both osteoclastogenesis and osteoblastogenesis. IL1β priming TMSCs also suppressed proliferation of peripheral blood mononuclear cells (PBMCs) and decreased expression of Receptor activator of nuclear factor kappa-Β ligand (RANKL) in PHA-stimulated PBMCs. Furthermore, osteoclast specific genes such as Nuclear factor of activated T cells c1 (NFATc1) were effectively down regulated when co-cultured with IL1β priming TMSCs in RANKL induced osteoclasts. In OVX mice, IL1β priming TMSCs induced low level of serum RANKL/osteoprotegerin (OPG) ratio on the first day of the last administration. Four weeks after the last administration, bone mineral density and serum Gla-osteocalcin were increased in IL1β priming TMSC-treated OVX mice. Furthermore, bone formation and bone resorption markers that had been decreased in OVX mice with low calcium diet were recovered by infusion of IL1β priming TMSCs. Conclusion: IL1β priming can endow constant therapeutic efficacy with TMSCs, which may contribute to improve bone density and maintain bone homeostasis in postmenopausal osteoporosis. Therefore, IL1β priming TMSCs can be a new therapeutic option for treating postmenopausal osteoporosis. Background: Stem cell therapies can be a new therapeutic strategy that may rebalance anabolic and anti-resorptive effects in osteoporosis patients. Tonsil-derived mesenchymal stem cells (TMSCs) can be an alternative therapeutic source for chronic degenerative diseases including osteoporosis. MSCs acquire immune regulatory function under the inflammatory cytokines. Since interleukin (IL) 1β is known to be one of inflammatory cytokines involved in osteoporosis progression, treatment of IL1β with TMSCs may enhance immunomodulatory function and therapeutic effects of TMSCs in osteoporosis. Methods: For IL1β priming, TMSCs were cultured in the presence of the medium containing IL1β for 1 day. Characteristics of IL1β priming TMSCs such as multipotent differentiation properties, anti-inflammatory potential, and suppression of osteoclast differentiation were assessed in vitro. For in vivo efficacy study, IL1β priming TMSCs were intravenously infused twice with ovariectomized (OVX) osteoporosis mouse model, and blood serum and bone parameters from micro computed tomography images were analyzed. Results: IL1β priming TMSCs had an enhanced osteogenic differentiation and secreted factors that regulate both osteoclastogenesis and osteoblastogenesis. IL1β priming TMSCs also suppressed proliferation of peripheral blood mononuclear cells (PBMCs) and decreased expression of Receptor activator of nuclear factor kappa-Β ligand (RANKL) in PHA-stimulated PBMCs. Furthermore, osteoclast specific genes such as Nuclear factor of activated T cells c1 (NFATc1) were effectively down regulated when co-cultured with IL1β priming TMSCs in RANKL induced osteoclasts. In OVX mice, IL1β priming TMSCs induced low level of serum RANKL/osteoprotegerin (OPG) ratio on the first day of the last administration. Four weeks after the last administration, bone mineral density and serum Gla-osteocalcin were increased in IL1β priming TMSC-treated OVX mice. Furthermore, bone formation and bone resorption markers that had been decreased in OVX mice with low calcium diet were recovered by infusion of IL1β priming TMSCs. Conclusion: IL1β priming can endow constant therapeutic efficacy with TMSCs, which may contribute to improve bone density and maintain bone homeostasis in postmenopausal osteoporosis. Therefore, IL1β priming TMSCs can be a new therapeutic option for treating postmenopausal osteoporosis.

Isolation of Alkaloids with Immune Stimulating Activity from Oryza sativa cv. Heugnambyeo

유민주,정하숙,Ryu, Min-Ju,Chung, Ha-Sook Korean Chemical Society 2010 대한화학회지 Vol.54 No.1

유색미인 흑남벼(Oryza sativa cv. Heugnambyeo) 미강의 ethylacetate 가용성 용매분획물로부터 NO 생성을 저해시키는 단일화합물을 순수 분리한 후, HREI-MS 및 2D-NMR 분석을 통해 화학구조를 4-carboethoxy-6-methoxy-2-quinolone (1)과 4-carboethoxy-6-hydroxy-2-quinolone (2)으로 명명하였다. We describe the immune stimulatory effects of compounds determined by means of activity-monitored extraction and isolation techniques. As a result, 4-carboethoxy-6-methoxy-2-quinolone (1) and 4-carboethoxy-6-hydroxy-2-quinolone (2) were isolated from the ethyl acetate-soluble fraction of the Oryza sativa cv. Heugnambyeo bran, and were determined to exert significant inhibitory effects in macrophage cell line (murine RAW 264.7) and murine splenocytes. The structures were elucidated on the basis of spectroscopic evidence, particularly the results obtained via hetero nuclear multiple-bond connectivity and high-resolution MS spectroscopy. Up to date, compound (1) was isolated as natural sources for the first time.

오미자 열수추출물의 대장암세포 증식억제 효과

유민주;정하숙 덕성여자대학교 대학원 2011 덕성여자대학교 대학원 논문집 Vol.13 No.-

This study investigated anticancer activity of Schizandra chinensis Baillon for the evaluation as a functional food resources. Schizandra chinensis Baillon were extracted with water for 3 hr at 90 °C. In the present study, we investigated the antiproliferative activity of the water extract of Schizandra chinensis Baillon in human colon cancer cell line(HT-29) via cell viability assay, morphology study, cell cycle analysis and RT-PCR. The HT-29 cells were cultured in the presence of several concentrations(0, 1.0, 2.0, 4.0 mg/mL) of water extracts of Schizandra chinensis Baillon. As a result, Schizandra chinensis Baillon could inhibit the colon cell growth in a dose-dependent manners, which was associated with morphological changes and apoptotic cell death with cell shrinking, chromatin condensation and apoptotic bodies. In cell cycle analysis, treatment with Schizandra chinensis Baillon resulted in marked increases of colon cells in the GO(sub-Gl) and decrease in the G0/G1 phases. And p53 mRNA expression were increased. These results suggest that Schizandra chinensis Baillon inhibits the growth of human colon cancer cells by various apoptosis-aiding activities as well as apoptosis itself.

부모교육 통합프로그램이 장애아동 어머니의 양육태도와 양육스트레스에 미치는 효과

유민주 ( Min Joo Yoo ),선우현 ( Sun Woo Hyun ) 한국정서ㆍ행동장애아교육학회 2009 정서ㆍ행동장애연구 Vol.25 No.2

오미자 열수추출물의 대장암세포 증식억제 효과

유민주,정하숙 한국산업식품공학회 2011 산업 식품공학 Vol.15 No.1

본 실험에서는 대장암 세포 HT-29의 증식을 억제하고 세포 사멸을 유도하는 천연소재 발굴을 목적으로, 오미자(Schizandra chinensis Baillon) 열수 추출물을 이용하여 인체 대장암 세포 HT-29의증식에 미치는 영향을 확인하였다. 오미자 열수 추출물이 HT-29 대장암 세포의 apoptosis 유도 효과 및 기전에 미치는 영향을 분자생물학적 방법으로 실험하여 다음과 같은 결론을 얻었다. MTT assay를 통해 인체 대장암세포 HT-29는 오미자 시료농도 0, 1.0, 2.0, 4.0 mg/mL에서 암세포 사멸농도가 각각 0%, 10%, 70%, 88%를 나타내었다. 대장암세포에 오미자 추출물을 처리하고 cell cycle 분석 결과, 시료농도 의존적으로 sub-G1기가 증가하였고, G0/G1기는 감소되는 것을 통해, apoptosis가 일어나 세포 증식을 저해하는 것으로 확인되었다. 대장암세포 핵의 형태학적 변화를 보면, 오미자 추출물 처리 시 농도 의존적으로 세포수가 감소되는 것이 뚜렷이 관찰되었고 cell shrinking, chromatin condensation 등 apototic body 등과 같은 형태학적 변화들이 뚜렷하게 관찰되었다. RT- PCR을 통한 유전자 발현은, 오미자 추출물 농도 의존적으로 p53 유전자 발현이 증가되는 것을 통해 대장암세포의 증식억제를 확인할 수 있었다. In vitro 실험에서 오미자 열수추출물이 대장암세포의 성장을 저해하는 효과가 있음을 확인하였으며, 오미자 추출물에 함유된 활성 본체 규명 및 apoptosis를 유도하는 작용기작에 관한 연구가 수행중이다. The anticancer activity of Schizandra chinensis Baillon was investigated for the development of functional food resources. The antiproliferative activity of hot water extracts of Schizandra chinensis Baillon in human colon cancer cell line (HT-29) were identified using cell viability, morphology study, cell cycle and RT-PCR analyses. HT-29 cells were cultured in several concentrations (0, 1.0, 2.0, 4.0 mg/mL) of water extracts of Schizandra chinensis Baillon. In our study, colon cancer cell growth could be inhibited by hot water extracts of Schizandra chinensis Baillon in a dose-dependent manners. It was associated with morphological changes and apoptotic cell death with cell shrinking, chromatin condensation, apoptotic bodies and cell cycle analysis. These results suggest that Schizandra chinensis Baillon may inhibit the growth of human colon cancer cells by various apoptosis-aiding activities as well as apoptosis itself.

오미자 열수추출물의 대장암세포 증식억제 효과

유민주 ( Min Ju Ryu ),정하숙 ( Ha Sook Chung ) 한국산업식품공학회 2011 산업 식품공학 Vol.15 No.1

본 실험에서는 대장암 세포 HT-29의 증식을 억제하고 세포 사멸을 유도하는 천연소재 발굴을 목적으로, 오미자 (Schizandra chinensis Baillon) 열수 추출물을 이용하여 인체 대장암 세포 HT-29의 증식에 미치는 영향을 확인하였다. 오미자 열수 추출물이 HT-29 대장암 세포의 apoptosis 유도 효과 및 기전에 미치는 영향을 분자생물학적 방법으로 실험하여 다음과 같은 결론을 얻었다. MTT assay를 통해 인체 대장암세포 HT-29는 오미자 시료농도 0, 1.0, 2.0, 4.0 mg/mL에서 암세포 사멸농도가 각각 0%, 10%, 70%, 88%를 나타내었다. 대장암세포에 오미자 추출물을 처리하고 cell cycle 분석 결과, 시료농도의 존적으로 sub-G1기가 증가하였고, G0/G1기는 감소되는 것을 통해, apoptosis가 일어나 세포 증식을 저해하는 것으로 확인되었다. 대장암세포 핵의 형태학적 변화를 보면, 오미자 추출물 처리 시농도 의존적으로 세포수가 감소되는 것이 뚜렷이 관찰되었고 cell shrinking, chromatin condensation 등 apototic body 등과 같은 형태학적 변화들이 뚜렷하게 관찰되었다. RT- PCR을 통한 유전자 발현은, 오미자 추출물 농도 의존적으로 p53 유전자 발현이 증가되는 것을 통해 대장암세포의 증식억제를 확인할 수 있었다. In vitro 실험에서 오미자 열수추출물이 대장암세포의 성장을 저해하는 효과가 있음을 확인하였으며, 오미자 추출물에 함유된 활성 본체 규명 및 apoptosis를 유도하는 작용기작에 관한 연구가 수행중이다. The anticancer activity of Schizandra chinensis Baillon was investigated for the development of functional food resources. The antiproliferative activity of hot water extracts of Schizandra chinensis Baillon in human colon cancer cell line (HT-29) were identified using cell viability, morphology study, cell cycle and RT-PCR analyses. HT-29 cells were cultured in several concentrations (0, 1.0, 2.0, 4.0 mg/mL) of water extracts of Schizandra chinensis Baillon. In our study, colon cancer cell growth could be inhibited by hot water extracts of Schizandra chinensis Baillon in a dose-dependent manners. It was associated with morphological changes and apoptotic cell death with cell shrinking, chromatin condensation, apoptotic bodies and cell cycle analysis. These results suggest that Schizandra chinensis Baillon may inhibit the growth of human colon cancer cells by various apoptosis-aiding activities as well as apoptosis itself.

단거리 육상선수와 일반여대생의 신체구성, 골밀도와 골밀도 관련 요인 분석

유민주(Min Ju Rhyu),오수일(Su Ill Oh) 한국여성체육학회 2011 한국여성체육학회지 Vol.25 No.3

The purpose of this study was to establish the effect of long-term running exercise as compared with bone mineral density, related hormones(estrogen, osteocalcin, calcitonin, parathyroid hormone(PTH)), vitamin D level and amount of sunshine of runners(n=10) and students(n=10) in a college. The data of study were analyzed by t-test,(and 생략) correlation analysis and regression analysis by stepwise, and the alpha level of α=.05 was set for all test of significance. The conclusion of the study were as follows. The runner group`s muscle mass and lean body mass of body composition, lumber spine(L2, L3, L4, total) and femur spine(neck, upperneck, wards, troch, shaft, total) of bone mineral density, vitamin D level and amount of sunshine were significantly higher than the control group`s. There were significant relationship between lumber spine and weight, muscle mass and lean body mass of body composition, between femur bones and musle mass and lean body mass of body composition and between lumber spine and femur bones with amount of sunshine.

은행잎 추출물로부터 Flavonoid 분리 및 대장암세포의 Apoptosis 유도

서진아;유민주;고은희;정하숙 덕성여자대학교 자연과학연구소 2012 자연과학 논문집 Vol.18 No.-

This study was conducted on isolation of pure compound(1) from 80% methanol extracts of Gingko biloba L. leaves through silica gel open column chromatography. The chemical structure was identified by ^1H-NMR and ^13C-NMR spectroscopic analysis. The anti-proliferation activity of Ginkgo biloba L. leaves on HCT116 human colon cancer cells was investigated. It was found that 80% methanol extracts of Ginkgo biloba L. inhibits HCT116 cell growth in a dose-dependent manner by MTT and western blot assays. The results indicates that Ginkgo biloba L. extracts inhibit growth of HCT116 cells by the induction of apoptosis. It might be mediated by change the Bcl family proteins and increase the activation of caspase-3 and caspase-9.

유기농 茶와 주요 Catechins의 대장암세포 사멸효과

김민지;유민주;이범진;이정대;김영걸;정하숙 덕성여자대학교 자연과학연구소 2012 자연과학 논문집 Vol.18 No.-

건강한 삶을 오랫동안 유지하기 위한 현대인의 요구에 부응하며, 건강효능이 우수한 천연소재에 대한 연구가 꾸준히 지속되고 있다. 전 세계적으로 널리 음용되고 있는 차(Camellia sinensis L.)는 항산화, 항염증, 항암작용을 가진 phytochemicals이 다량 함유되어 있다. 본 연구에서는 대장암세포 성장을 억제하는 천연소재 발굴을 목적으로, 무발효차(우전, 세작, 중작, 대작)와 발효차{선향, 운향, 삼다연)롤 열수 추출물로 제조한 후 HT-29 세포증식 억제효과를 확인하였다. 또한 茶에 함유된 주요 catechins 성분의 함량을 분석하고, 함량이 높은 EGCG, ECG, EGC를 분리하고, 대장암세포 성장저해 효과를 규명하였다. 실험 결과, 무발효차의 경우 총 catechins 함량이 12.8%로 우전과, 발효차의 경우 8.01%로 선향이 가장 높았으며, catechins 중에서도EGCG가 가장 많이 함유되었다. HT-29 대장암세포증식은 중작, 선향과 운향이 200ug/mL농도에서부터 유의적으로 감소하였으며, 이는 catechins 함량이 높을수록 낮은 농도에서도 대장암세포 증식이 유의적으로 감소하였고, catechins 중 ECG가 가장 높은 사멸효과를 나타내었다.

Ring-Oscillator based Digital PLL with Fine TDC for FMCW LiDAR with 1us Lock Time in 28nm CMOS

Min-Joo Yoo(유민주),Seung-Ju Lee(이승주),Eui-Geun Kim(김의근),Joon-Hee Park(박준희),Jin-Wook Burm(범진욱) 대한전자공학회 2023 대한전자공학회 학술대회 Vol.2023 No.6