EXPRESSION , ISOLATION AND PROPERTIES OF FUR ( FERRIC UPTAKE REGULATION ) PROTEIN OF Escherichia coli K -12

위세찬,J . B . Neilands 한국생화학분자생물학회 (구 한국생화학회) 1987 추계학술대회집 Vol.- No.-

Aerbactin Operon 의 Operator 와 Fur ( Ferric Uptake Regulation ) 단백질간의 결합하는 성질

위세찬,최수영,손정인 ( Se Chan Wee,Soo Young Choi,Jeong In Sohn ) 생화학분자생물학회 1993 BMB Reports Vol.26 No.8

Binding properties of Fur protein to the operator region of aerobactin operon were investigated. By nitrocellulose filter binding assay, dissociation constant of Fur-operator complex was ranged 3∼6×10^(-12) M² using Mn^(2+) as a corepressor. Hill plot of the binding isotherm indicates that the repressor protein forms dimer in order to bind the operator. Stability of Fur-operator complex depends on the divalent transition metal involved in the complex. Binding affinity of the protein was not altered by the different incubation temperature. However, at higher temperature the repressor showed higher saturation level with the same amount of operator.

인삼추출물이 주정 대사에 미치는 영향 , 주정 투여쥐의 간장의 조직화학적 연구

위세찬,김봉용,백태홍,주충노 ( Sec Han Eui,Bong Yong Kim,Tai Hong Paik,Chung No Joo ) 생화학분자생물학회 1978 BMB Reports Vol.11 No.1

미생물 농약

위세찬 한국미생물학회 1988 微生物과 産業 Vol.14 No.2

이글은 siderophore의 식물과의 관계에 집중될것이다. 독자들은 Neilands와 Leong의 review에서 많은 도움을 얻으리라 믿는다. 한편 siderophore의 또다른 면모(관점)에 관심이 있는 독자를 위하여 몇가지 참고문헌을 제시하고자 한다. 미생물에 의해 생성되는 siderophore의 종류및 화학성분에 관한 것, 철분을 포함하는 항생물질에 관한 것들이 있고 철분에 의한 조절기작의 최근의 분자 생물학적 연구도 있다. 그외에 철분의 흡수과정에 필요한 유전적 배경에 관한 것도 있다.

생명과학기술의 현황과 전망-21세기의 생명과학기술

위세찬,Wi, Se-Chan 한국과학기술단체총연합회 1998 과학과 기술 Vol.31 No.3

생물산업(생명과학기술)은 정보산업과 함께 21세기를 주도할 대 산업중의 하나이다. 지난 수십년간 급속히 발달한 분자생물학과 유전공학에 힘입어 생명과학기술은 폭발적으로 팽창해왔다. 생명과학기술은 그동안 인슐린을 비롯해 성장호르몬, 혈약응고인자 등 세상을 놀라게 했다. '인간복제'를 둘러싸고 전 세계를 떠들썩하게 했던 이 생명과학기술에 새 세기엔 어떻게 펼쳐질 것인가 알아본다.

Aerobactin Operon의 Operator와 Fur(Ferric Uptake Regulation) 단백질간의 결합하는 성질

위세찬,최수영,손정인,Wee, Se-Chan,Choi, Soo-Young,Sohn, Jeong-In 생화학분자생물학회 1993 한국생화학회지 Vol.26 No.8

순수 분리된 Fur 단백질과 Aerobactin Operon의 작동자 부분을 포함하는 DNA 절편간의 결합하는 성질을 Nitrocellulose filter binding assay에 의하여 측정하였다. Fur 단백질은 $Fe^{2+}$ 이외에도 많은 전이 금속을 보조 억제 인자로서 수용하는 것으로 보인다. 얻어진 Fur-작동자 결합의 해리상수는 $Mn^{2+}$을 보조 억제 인자로 사용하였을 경우$(3{\sim}6){\times}10^{-12}M^{2}$이었으며 Fur 단백질은 Dimer로서 작동자와 결합하는 것으로 나타났다. Fur-작동자 결합을 $43^{\circ}C$에서 수행 하였을 때는 $23^{\circ}C$ 또는 $37^{\circ}C$ 때와 동일한 해리상수를 얻었다. 그러나 Fur 단백질은 고온에서는 동량으로 첨가된 작동자로부터 더 높은 포화 수치(saturation level)를 보여준다. Binding properties of Fur protein to the operator region of aerobactin operon were investigated. By nitrocellulose filter binding assay, dissociation constant of Fur-operator complex was ranged $(3{\sim}6){\times}10^{-12}M^{2}$ using $Mn^{2+}$ as a corepressor. Hill plot of the binding isotherm indicates that the repressor protein forms dimer in order to bind the operator. Stability of Fur-operator complex depends on the divalent transition metal involved in the complex. Binding affinity of the protein was not altered by the different incubation temperature. However, at higher temperature the repressor showed higher saturation level with the same amount of operator.

Sequence of Thyroxine - Binding Globulin Gene in a Korean Family with Complete TBG Deficiency

위세찬,최의열,백창호,장연복 한국유전학회 1996 Genes & Genomics Vol.18 No.3

Genomic DNAs from an 11 year-old boy with suspected complete thyroxine-binding globulin deficiency(TBG-CD) and a normal individual were subjected to polymerase chain reactions and sequenced. The entire coding region and exon/intron boundary of TBG gene from a normal individual showed no base change in exons as reported. However, sequence of the TBG gene from the suspected individual revealed a single nucleotide deletion at codon 352 in exon 4. The mutation was further confirmed by allele-specific amplification of genomic DNAs from the affected hemizygous boy, a hemizygous normal male, and the heterozygous boy's mother with primers containing 3' end nucleotide missing in TBG-CD or normal sequence. Several distinct mutations in the coding regions of the TBG gene have been shown to be associated with TBG-CD. The Korean type identified in this study was found to be the same mutation type of TBG-CD in Japanese. In addition, we identified 3 more base changes in introns both in normal and TBG-CD subjects.

The Effect of Ginseng on Alcohol Metabolism A Histochemical Study of Alcohol Fed Rat Liver

위세찬,김봉용,백태홍,주충노,Eui, Se-Chan,Kim, Bong-Yong,Paik, Tai-Hong,Joo, Chung-No 생화학분자생물학회 1978 한국생화학회지 Vol.11 No.1

In the view of the widespread human consumption of ethanol and its toxic effect on liver, it was attempted in this study to observe the effect of the total extract of Panax ginseng roots on the liver of rats fed with 12% ethanol prior to ginseng extract administration and the following results were obtained. 1) When mice or rats were fed with ginseng extract containing diet, the rate of body weight increase was found higher than that of control group. 2) When rats was free access to 12% ethanol instead of water, their body weight increase rate was lowered. When alcohol dosed animals were fed with 2% ginseng extract containing diet, their body weight increase was found faster than that of control animal. 3) A photomicroscopic study of the liver section of alcohol fed rats showed that 4-10 days' alcohol feeding did not cause severe hepatic injury but longer (more than 14 days) period of alcohol feeding injured the liver cells. 4) A histochemical study of hepatocytes of the above animals showed that administration of ginseng extract for 58 days after 14 days' alcohol feeding modified the liver injury (fatty degeneration and glycogen deposition). 5) Analysis of hepatic protein, sugar and lipid of the above animals under different feeding conditions showed that alcohol feeding lowered the protein composition and caused sugar and lipid deposition significantly but ginseng extract helped to maintain the normal levels of the above components. 6) The above results led us to conclude that the ginseng extract has remarkable effects of protecting the liver from alcohol intoxication. 인간 사회에서 애용되는 주정이 간장에 미치는 독성을 고려하여 인삼 추출물과 12% 에탄올을 투여한 쥐간에 미치는 영향을 관찰하고 다음과 같은 실험 결과를 얻었다. 1) 생쥐나 백서에 인삼 추출물을 함유한 사료를 투여하면 체중의 증가율이 대조군에 비하여 높다. 2) 쥐에게 물대신 12% 에탄올을 자유로이 먹게하면 그의 체중증가율이 낮아진다. 그러나, 주정을 투여한 쥐에게 인삼 추출물이 함유된 사료를 먹이면 그들의 체중 증가율은 대조군보다 빠르다. 3) 4일~10일간 주정을 투여한 쥐의 간 조직의 현미경적 고찰에 의하면 그다지 큰 간세포의 변화를 관찰하기 어려우나 오랫동안(14일 이상) 알코올을 투여하면 간 세포의 장해상이 관찰된다. 4) 쥐의 간 세포의 조직화학적 관찰 결과 14일간 주정을 투여한 후 58일간 2%인삼 추출물이 함유된 사료를 투여하면 상해진 간 세포가 거의 정상으로 회복된다. 5) 여러가지 사료 투여조건이 다른 쥐의 간장의 단백질, 당류, 지방질을 분석한 결과 주정 투여로 인하여 간장의 단백질 성분이 저하되고, 당과 지방질이 상당량 침체되지만 인삼 추출물은 위의 성분들을 정상수준으로 유지하는 데 효과가 있는 것으로 관찰 되었다. 6) 위의 결과로 부터 인삼 추출물은 알코올 독성으로 부터 간을 보호하는데 상당한 효과가 있는 것으로 생각된다.

글루탐산 생성 박테리아에 관한 생화학적 연구

김택영,위세찬 ( Taik Young Kim,Se Chan Wee 생화학분자생물학회 1975 BMB Reports Vol.8 No.3

Isocitrate lyase and malate synthetase were found to be active in Brevibacterium anamoniagenes grown on the acetate media, indicating that the glyoxylate cycle was operated. The specific activity of the isocitrate lyase from the acetate media showed about 70 times greater than that from the glucose media. The optimum pH for the partially purified isocitrate lyase was 7.0∼7.2 and Km value for the DL-isocitrate was determined as 0.41 mM. The effects of divalent ions were studied. Magnesium ion was an effective activator, but calcium, cobalt, cupric, ferrous, and manganese ions were found to precipitate in the assay mixtures. Iodoacetate, p-hydroxymercuribenzoate and phosphoenolpyruvate were the strong inhibitors for the enzyme. Phosphoenolpyruvate inhibited the enzyme to 78% at pH 6.85 and 47% at pH 7.25. A possible mechanism of the phosphoenolpyruvate to the regulation of the glyoxylate cycle was discussed.

GABA transaminase 의 새로운 보조인자 유사체로서의 6 - Br - pyridoxal - 5 - phosphate

최수영,위세찬,김두식 ( Soo Young Choi,Sechan Wee,Doo Sik Kim ) 생화학분자생물학회 1989 BMB Reports Vol.22 No.2

6-Br-pyridoxal-5-phosphate, a new cofactor analog of pyridoxal-5-phosphate, was synthesized and purified homogeneously by organic and enzymatic methods. This cofactor analog binds to cofactor binding site of GABA transaminase. Resolved and reduced GABA transaminase restore the catalytic activity by 6-Br-PLP. These results indicate that the 6-Br-PLP remains covalently attached to the amino acid residue of the catalytic site and acts like natural cofactor.