간의 지방섭취세포의 구조와 기능

원봉래 대한수의사회 1981 대한수의사회지 Vol.17 No.4

FSC는 상시지방적을 보유하는 특성을 가지고 있으므로 지방섭취세포(fat-storing cell, FSC)라고 명명되어으며 유동에 노출하는 성세포, 내피세포와 달리 이 세포의 유동면은 교원직유와 내피세포에서 유래하는 윤랑선에 의하여 피복된다. 더욱이 이 세포는 각종 척추동물에 존재한다는 사실이 확인 되었으며 그 지방적은 동물의 종류에 따라 특유한 형을 구비하는 동시에 개체차가 없다. FSC는 이물탐식성이 없으며 지방적을 함유한 성세포와는 위치적, 기능적으로 다르다는 것이 증명되었다. 발생학적으로는 간엽세포에 속하며 유사분제에 의하여 증식능력을 가지고 있다. 이 세포에는 glycogen이 증명되며 모든 지방세포의 실험이 나타나는 성적에서와 유사하게 항시 지방을 합성하여 종의 특유한 형을 가진 지방적으로서 저장됨을 시사하였다. 전현적관륜에 의하여 FSC가 유동주위강(disse腔) 속에 있어 성세포, 내피세포와는 달리 유동에 노출하지 않고 항상 내피세포층과 교원직유에 의하여 유동에서 격리됨을 추정하였다. 이로 인하여 많은 학자들에 의하여 FSC가 승인을 받았다. 오늘날에는 유동을 둘러싸고 있는 상재세포가 내피세포, 성세포 및 FSC의 3종임이 인증이 되었다. 이 세포의 미세구조상의 특징은 기저막이 없는 공허한 세포로 보이나 항상지방적(空胞)을 보유하고 잘 발달한 조면소포체를 가지며 유동내피면에 따라 분기확산하는 돌기를 가지고 있다. 그리고 세포체는 유동주위강을 달리는 무수신경직유의 varicosity (사립체 및 신경결합소포를 함유한 신경종말)와의 사이에 adrenalin 작동성 synaps를 형성한다. FSC의 기능적 의의는 ㄱ, 간소엽내 교원직유형성에 참여 ㄴ. 유동내피하 돌기는 내피를 외측에서 지지하고 보강하며 또한 유동을 둘러싸고 연장하는 돌기는 수축하여 유동강을 축소한다. ㄷ. 지방을 저장하여 간세포의 energe원을 공급하며 VitaminA를 그 속에 저장한다. ㄹ. 간의 해독작용에 관여한다.

쥐의 대뇌동맥윤에 관한 연구

원봉래,이재현 COLLEGE OF AGRICULTURE KYUNGPOOK NATIONAL UNIVERSI 1984 慶北大農學誌 Vol.2 No.-

Little has been reported concerning circulus arterious cerebri in adult rats. Certain morphological features not described previously were observed. The more significant of results these were as follows: The asymmetrical origin of the posterior cerebral artery was found to occur in more than half of the animals. Presence of cleft-like buttonhole formations along an anterior cerebral artery at one side or both sides was a feature that show practically the species characteristic. The supplemental anterior communicating artery was occasionally found at the place of fusioning with the two anterior cerebral arteries. Exceptionally, 3 cases of the anterior cerebral artery did not fuse to both arteries but they were unusually connected with the two anterior communicating arteries. An olfactory artery to the nasal cavity was prominently branched from the anterior cerebral artery in all animals.

Vinyl腐蝕法에 依한 在來山羊 脾柱靜脈의 區域的 走行에 關한 硏究

金鍾涉,金武剛,金容根,元鳳來 慶尙大學校 1972 論文集 Vol.11 No.-

The studies were conducted to observe the segmental running of splenic trabecular veins, employing the vinylite corrosion technique in sixty adult Korean goats, and the following results were obteined: 1. The splenic trabecular veins of the goat were collected into 5 chief Rami of the splenic veins(called Rr. superior, media et inferior, Vv. polares superior et inferior), and the splenic parenchyma was divided into 3-8 intralienal venous segments by the course of trabecular veins. 2. Each intralienal venous segment was divided into 2-4 minute segments, therefore 7-22 minute segments were observed in the spleen. 3. The anastomoses were found between segments or minute segments. 4. The splenic arterial and venous system of the Korean goat took a individual course in the splenic parenchyma respectively.

動物 子宮 粘膜上皮의 脫落 및 再生에 關한 電子顯徵鏡的 硏究

元鳳來 진주농과대학 1969 진주농과대학 연구논문집 Vol.- No.8

Electron microscopic studies were carried out on the uterine mucosa of the rats and cows, to compare the fine structures of epithelial cells of uterine mucosa at the different estrous cycles and to find out possible mechanism of the cell renewal and destruction of the uterine mucosa, and the results obtained were wummarized as follows. 1. Three different layers of epithelial cells were distinguished by their fine morphology particuly at diestrus. These were superficial, dividing and regenerating cell layers which were considered to be the rare incidence but firm morphological evidence for the renewal of the mucosa by direct division of basal cells existed in the mucosa. 2. Cytolysomes of various internal structures were increased in number at the later stage of the cycle in the rats and were considered to be the product of cytolytic activitic of the cells, and suggested that increased number of cytolysomes may lead the cell death. So-called subterminal bars were present at the luminal region of the cells, being paralled to the luminal border and increased in their extent and were segregated from the cytoplasm at the later stage, suggesting mode of the cell destruction on the cow uterine mucosa. 3. Microvilli at the luminal border of the superficial epithelial cells were short and sparse at the proestrus but at estrus and metestrus, these were increased in length and number. 4. Antennulae microvilares radiating from the microvilli were abundant at proestrus but were decreased at estrus and metestrus. 5. In estrus and metestrus, mitochondria of the superficial epithelial cells increased remarkably in their sizes and numbers. 6. Multivesicular bodies and lysosome were also common cytoplasmic organelles present in the cells at any estrus stages, although the former were noticiably increased in number at metestrus stage. 7. Endoplasmic reticulum, both rough and smooth, were found to be increased in their extent, at estrus and metestrus, although smooth endoplasmic reticulum was prominent at the basal region of the cells and it was close approximation to the lipid droplets. 8. Golgi complex of the superficial epithelial cells showed the tendency to increase its extent as the estrus cycle proceeded from proestrus to metestrus. 9. In all stages of the estrous cycle, lipid droplets were observed in the cytoplasm of the epithelial cells although their numbers, sige and distribution were somewhat varied at the different estrous cycles; at the proestrus and estrus states relatively small lipid droplets were present in the basal region of the cell, but at metestrus, their size was increased and seen at the luminal cytoplsm of thec ells. The presence of the lipid droplets in the cytoplasm of the cells at any stages could be related to physiological cellular activities rather than to degenerative process of the cells.

고양이의 胸壁動脈에 關한 硏究 : II The Lateral internal thoracic artery and intercostal artery 第Ⅱ報 外側內胸動脈과 肋間動脈에 關하여

元鳳來 진주농과대학 1967 진주농과대학 연구논문집 Vol.- No.6

The author has studied the origin and distribution of the lateral internal thoracic artery and intercostal artery by using thirty-six cats. The results obtained are as follows: 1. The aortic arch generally is on the 4th thoracic vertebra, but aortic arch sometimes is on the 2nd, 3rd, 5th or 6th thoracic vertebra. 2. The lateral internal thoracic artery generally arises from the deep cervical artery, subclvian artery, or near the origin of the costocervical trunk, but sometimes it originates from the vertebral artery or the internal thoracic artery. 3. The lateral internal thoracic artery is generally distributed over the muscles of the intercostal spaces, from the Ist to the 2nd or only Ist intercostal space, but sometimes from the Ist to the 3rd. 4. The Ist intercostal artery is small artery, which arises from the deep cervical artery, but it is often absent. (Fig. 4.(1, 2, 10 13, 16)) 5. The 2nd and 3rd intercostal arteries generally arise from the supreme intercostal artery, but these do not always arise form the supreme intercostal artery. Sometimes these arteries arise from the thoracic aorta directly. 6. The 4th intercostal artery generally arise from the thoracic aorta directly, but it does not always arise from the thoracic aorta directly. Sometimes it arises from the supreme intercostal artery. 7. Intercostal arteries, being from 5th to 13th intercostal spaces, arise from the thoracic aorta directly. 8. The cephalic and caudal intercostal arteries which originate directly in the thoracic aorta often arise from the common trunk, while the intermediate arteries (7th-11th intercostal arteries) arise directly from the thoracic aorta. Their branches run cranially in the cephalic region and caudally in the caudal region from the common trunk. 9. When the intercostal arteries have a common trunk, the branch runs over (Fig. 4. (1, 2, 5, 7, 8, 15, 16)) or under (Fig. 4. (2, 3, 4, 5, 8, 11, 12)) the neck of the rib. 10. The left intercostal artery mostly arises more cepharically than the right, but somtimes the left and the right interostal arteries originate in the same level. 11. Sometimes the supreme intercostal artery is absent (Fig. 4. (16)) ; in this case all intercostal arteries except only the Ist intercostal artery arise from the thoracic aorta.

牛 子宮 粘膜 細胞의 Ca45 攝取에 미치는 Steroid Hormone의 影響

元鳳來 진주농과대학 1968 진주농과대학 연구논문집 Vol.- No.7

1. Ca45 攝取는 生體 內에 있어서 子宮 粘膜 細胞에 特히 顯著하다. 2. Ca45 攝取는 特히 子宮 粘膜 細胞의 mitochondria 分劃에 特異的으로 많이 存在한다. 3. Ca45 攝取는 子宮 mitochondria에 있어서는 性周期性이 있으나 肝에 있어서는 없다. 4. Ca45 攝取는 子宮 mitochondria에 있어서 ATP 依存性이 크나 肝에 있어서는 大端치 않다. 5. 2.4-DNP에 의한 Ca45 攝取는 阻害는 子宮 mitochondria에 있어서 크나 肝에 있어서는 그렇지 않다. 6. eistrone은 子宮 mitochondria, 肝 mitochondria 共히 Ca45의 攝取에 影響이 없다. 7. Progestrone은 子宮 mitochondria에의 Ca45 攝取를 强하게 阻害하나 肝 mitochondria에 있어서는 그렇지 않다. Experiments were Carried out to observe the effects of the steriod hormone in uptake of Ca45 by the cell of the endometrium in the cattle. The results obtained are summerized as follows. 1. The endometrium had the hightest uptake rates of Ca45 in the cattle. 2. The concentration of Ca45 shown in the fraction of the mitochondria of the endometrium was higher than the others. 3. The mitochondria of the endometirum had close relation ship with sexual cycle in the uptake of Ca45, but not, in the liver. 4. Ca45 uptake rates by the mitochondria of the endometrium depended highly upon the A.T.P. 5. 2,4-DNP considerably disturbod uptake of Ca45 by the mitochondria of the endometrium, but not in the liver. 6. There were no effects in the uptake of Ca45 in the mitochondria of endometrium and mitochondria of the liver by estrogen. 7. Progestrone disturb the uptake of Ca45 by the mitochondria of endometrium, but not in the liver.

낭견(狼犬)의 교상(咬傷)에 의(依)한 광견병발생례(狂犬炳發生例)

형성해,원봉래,이규명,Hyung, Sung-Hae,Won, Bong-Ran,Lee, Kyu Myung 대한수의학회 1962 大韓獸醫學會誌 Vol.2 No.2

In June, 1957, a rabid wolf near Chinju, Korea attacked and bit 18 persons, and the resulting epidemiologic observations are summarized as follows: (1) Five of 18 persons bitten died of rabies. (2) Ten persons were exposed by bites on the head, of which 5 received Pasteur treatment, and 5 did not. The rabies mortality ratios for these 2 groups were 5:3, and 5:1 respectively. (3) Of 8 persons bitten on the extremities, only one of 5 not receiving Pasteur treatment died of rabies. (4) The incubation period lasted 13 to 17 days, and the clinical course 1 to 2 days.

인삼투여가토(人蔘投與家兎) 간지방섭취세포(肝脂肪攝取細胞)의 전자현미경적관찰(電子顯微鏡的觀察)

이재현,원봉래,Lee, Jae-Hyun,Woun, Bong-Rae 대한수의학회 1980 大韓獸醫學會誌 Vol.20 No.2

The light and electron microscopic observations were carried out in order to know the morphological changes of the fat-storing cells (FSC) in the liver of rabbits given Ginseng for 30 days. The results obtained were as follows: The FSC of experimental group was shown the appearance of a few large lipid droplets, enlarged rough surfaced endoplasmic reticulum, some residual body in the enlarged rER and the nuclear body in some cases. As these findings, it could be suggested that the Ginseng affect the synthesis and accumulation of lipid on the FSC.

사염화탄소(四鹽化炭素)에 의한 간상해시(肝傷害時) 인삼(人蔘)이 간세포(肝細胞)의 미세구조(微細構造)에 미치는 영향(影響)

이재현,원봉래,이차수,Lee, Jae-Hyun,Woun, Bong-Rae,Lee, Cha Soo 대한수의학회 1978 大韓獸醫學會誌 Vol.18 No.2

Election microscopic investigation were conducted on the mature mouse (B.W. about 25g) liver which was treated with Ginseng water extract during three, six and nine days respectivelly after carbon tetracholride injection into abdominal cavity. The results obtained were as follows: The liver cells of control group treated with carbon tetrachloride alone were restored slowly. The liver cells of experimental group treated with Ginseng water extract after carbon tetrachloride injection, however, were shown the appearance of well-developed ${\gamma}-ER$ and Golgi complex, marked aggregation of glycogen particles, and a number of large lipid droplets which are attached markedly with glycogen particles as compared to the control group. As these findings, it could be suggested that Ginseng gives an activation to restore the damaged liver cells.

닭(鷄)의 脊柱骨長 成長에 關한 硏究

毛麒喆,元鳳來 慶北大學校 1977 論文集 Vol.24 No.-

As for the growth analysis of vertebral column in chicken, authors measured each vertebrae of one step per ten chickens in divided five steps each 15-days interval from newborn chicken to sixtieth day, and analized that measured result by means of growth formula and Huzlay's relative grwth rate law. The results obtained in this studies were summarized as follows: 1. Q. G. was most quickly increased in cervical vertebrae, and most slowly increased in coccygeal vertebrae. 2. G. R. was decreased in all vertebrae except coccygeal vertebrae. 3. S. G. R. was decreased in all vertebrae and then cervical vertebrae was great and coccygeal vertebrae slight. 4. G. P. was decreased in all vertebrae except coccygeal vertebrae.