Effect of High Glucose on MUC5B Expression in Human Airway Epithelial Cells

예상백,최윤석,최요한,배창훈,김용운,박소영,송시연,김용대 대한이비인후과학회 2017 Clinical and Experimental Otorhinolaryngology Vol.10 No.1

Objectives. Excessive production of mucus results in plugging of the airway tract, which can increase morbidity and mortality in affected patients. In patients with diabetes, inflammatory airway disease appears with more frequent relapse and longer duration of symptoms. However, the effects of high glucose (HG) on the secretion of mucin in inflammatory respiratory diseases are not clear. Therefore, this study was conducted in order to investigate the effect and the brief signaling pathway of HG on MUC5B expression in human airway epithelial cells. Methods. The effect and signaling pathway of HG on MUC5B expression were investigated using reverse transcriptase-polymerase chain reaction (RT-PCR), real-time PCR, enzyme immunoassay, and immunoblot analysis with specific inhibitors and small interfering RNA. Results. HG increased MUC5B expression and epidermal growth factor receptor (EGFR) expression, and activated the phosphorylation of EGFR and p38 mitogen-activated protein kinase (MAPK). Pretreatment with EGFR inhibitor significantly attenuated the HG-induced phosphorylation of p38 MAPK, and pretreatments with p38 inhibitor or EGFR inhibitor significantly attenuated HG-induced MUC5B expression. In addition, knockdown of p38 MAPK by p38 MAPK siRNA significantly blocked HG-induced MUC5B expression. Conclusion. These findings suggest that HG induces MUC5B expression via the sequential activations of the EGFR/p38 MAPK signaling pathway in human airway epithelial cells.

전두와의 해부학적 변이와 전두동염:부비동 전산화단층촬영을 이용한 분석

우현재,예상백,배창훈,송시연,김용대 대한비과학회 2009 Journal of rhinology Vol.16 No.1

Background and Objectives:The frontal recess (FR) cells, including the frontal cell (FC), agger nasi cell (ANC), supraorbital ethmoid cell (SOC) and inter-frontal sinus septal cell (IFSSC), can interfere with the drainage system of the frontal sinus. We evaluated the relationship between the FR cells and the frontal sinusitis. Subjects and Methods:All paranasal sinus CT scans performed from July 2004 through June 2005 were reviewed. Of the 675 scans reviewed, 317 patients (634 sides) were selected for data collection. Exclusion criteria included a clinical history of neoplasms, bony deformities, and extensive disease responsible for obscuring the bony anatomy. Similarly, CT scans with severe artifacts were also excluded. Result:FCs were present in 21.9% of the patients. According to the Bent’s classification, the prevalence of each FC type was as follows; type 1 FC (13.6%), type 2 FC (3.2%), type 3 FC (1.9%) and type 4 FC (3.1%). Of the four types of FR cells, type 4 had a significant association with frontal sinusitis. The incidence of hyperpneumatized frontal sinus, ANC, SOC, IFSSC were significantly higher among patients with the FC than those without the FC (p<0.05). Patients without the ANC or with type 1 uncinate process, according to Stammberger’s classification, displayed a higher frequency of frontal sinusitis (p<0.05). There was a statistically significant decrease in the frequency of frontal sinusitis among patients with hypopneumatized frontal sinus (p<0.05). Conclusion:In our series, the frontal sinusitis was influenced by each types of FC, attachment sites of uncinate process and the degree of frontal sinus pneumatization. Therefore, these anatomic variations in the frontal recess should be appropriately addressed during the surgical management of the frontal sinusitis. Background and Objectives:The frontal recess (FR) cells, including the frontal cell (FC), agger nasi cell (ANC), supraorbital ethmoid cell (SOC) and inter-frontal sinus septal cell (IFSSC), can interfere with the drainage system of the frontal sinus. We evaluated the relationship between the FR cells and the frontal sinusitis. Subjects and Methods:All paranasal sinus CT scans performed from July 2004 through June 2005 were reviewed. Of the 675 scans reviewed, 317 patients (634 sides) were selected for data collection. Exclusion criteria included a clinical history of neoplasms, bony deformities, and extensive disease responsible for obscuring the bony anatomy. Similarly, CT scans with severe artifacts were also excluded. Result:FCs were present in 21.9% of the patients. According to the Bent’s classification, the prevalence of each FC type was as follows; type 1 FC (13.6%), type 2 FC (3.2%), type 3 FC (1.9%) and type 4 FC (3.1%). Of the four types of FR cells, type 4 had a significant association with frontal sinusitis. The incidence of hyperpneumatized frontal sinus, ANC, SOC, IFSSC were significantly higher among patients with the FC than those without the FC (p<0.05). Patients without the ANC or with type 1 uncinate process, according to Stammberger’s classification, displayed a higher frequency of frontal sinusitis (p<0.05). There was a statistically significant decrease in the frequency of frontal sinusitis among patients with hypopneumatized frontal sinus (p<0.05). Conclusion:In our series, the frontal sinusitis was influenced by each types of FC, attachment sites of uncinate process and the degree of frontal sinus pneumatization. Therefore, these anatomic variations in the frontal recess should be appropriately addressed during the surgical management of the frontal sinusitis.

안와내벽 외향골절의 비내시경적 치료

배창훈,예상백,박강식,송시연,김용대 대한이비인후과학회 2005 대한이비인후과학회지 두경부외과학 Vol.48 No.12

Background and Objectives:Before introducing transnasal endoscopic sinus surgery, surgical repair of the blowout fracture includes the transorbital approach with an external incision and/or the maxilloethmoidal approach. The advantages of the transnasal endoscopic technique include magnified direct visualization and easy access to the medial orbital walls. Gelfoam is a slowly absorbable material and is rigid enough to provide support where Silastic sheet is to be placed. The purpose of this study was to evaluate outcomes of transnasal endoscopic reconstruction with Gelfoam and Silastic sheet of medial orbital wall fracture. Subjects and Method:This study was a retrospective analysis 27 patients who underwent transnasal endoscopic reduction of blowout fracture from February 1999 to April 2004. The follow-up period was at least over than 12 months. Results:The study population included 18 males and 9 female patients ranging from 13 to 54 years (mean, 33.3 years) of age. Twenty of 23 patients with diplopia showed a complete improvement of diplopia and 3 patients showed an incomplete improvement. All of 13 patients with limitation of ocular movement and 2 patients with enophthalmos revealed a complete resolution of their symptoms. There were no significant complications in all patients although ocular pain and epiphora were observed temporally after operation. Conclusion:Transnasal endoscopic reconstruction with Gelfoam and Silastic sheet for medial orbital wall fracture is a very successful technique that does not accompany major complications and has definite cosmetic advantage of causing no external scarring.

제라늄 에센셜 오일이 후각자극을 통해 식이섭취의 조절에 미치는 효과

최승재,예상백,나형균,김귀옥,배창훈,송시연,김용운,박소영,김용대 대한이비인후과학회 2011 대한이비인후과학회지 두경부외과학 Vol.54 No.11

Background and Objectives Recently, obesity has become one of the major health problems in our society. To overcome this problem, keeping a balance between food intake and energy expenditure is very important. Many natural substances including essential oils have been suggested for their potential effect on reducing weight. This study was performed to evaluate whether aroma inhalation of essential oil has a role in appetite regulation and works on the central nervous system through the olfactory stimulus. Materials and Method Food intake was measured after 30 minutes of treatment with essential oil in overnight fasted Sprague-Dawley rats. In the control group, saline was used instead of essential oil. Changes in pro-opiomelanocortin (POMC) and neuropeptide Y (NPY) mRNA expression levels in the hypothalamus were measured following 30 minutes of treatment with geranium essential oil using real-time polymerase chain reaction. Results Of the seven essential oils, geranium significantly decreased the amount of food intake compared to the control group. Geranium essential oil significantly increased POMC mRNA expression in the hypothalamus, but did not change the NPY mRNA expression. The increased POMC mRNA expression was reversed by treatment with xylocaine, which blocks the olfactory perception. Conclusion These results suggest that geranium essential oil has anorexic effect through the activation of POMC neurons in the hypothalamus via olfactory stimulus.

비인강암의 임상적 고찰과 예후인자

배창훈,서영중,예상백,최영호,김용대,송시연 영남대학교 의과대학 2005 Yeungnam University Journal of Medicine Vol.22 No.1

본 연구에서는 1993년 1월부터 2002년 12월까지 10년간 비인강암으로 진단받은 환자 54예를 환자의 의무기록과 전화상담을 통하여 후향 적으로 분석하여 비인강암의 임상적인 특징과 생존율에 미치는 인자에 대하여 알아보고자 하였다. 환자의 연령분포는 16~78세로 평균 연령은 46.9세였다. 40대(26.0%)와 50대(27.8%)에서 호발하였으며, 남자가 여자보다 3.5배 많았다. 경부 종물을 주소로 내원한 환자가 30예(55.5%)로 가장 많았으며, 조직학적 유형은 WHO 제3형이 29예(53.7%)로 가장 많은 분포를 나타내었다. 조직학적 유형에 따른 5년 생존율은 제1형은 40.0% 제 2형은 45.5% 제 3형은 54.1%였다. 비인강암의 발생위치는 Rosenmu¨ller fossa를 포함한 측벽에서 35예(64.8%)로 가장 많이 발생하였다. 진단 시 Ⅲ, Ⅳ기의 진행된 병기군이 79.6%로 비인강암은 비교적 늦게 발견됨을 알 수 있었다. 전체적인 5년 누적 생존율은 46.5%였으며, 초기 병기군과 진행된 병기군의 5년 누적 생존율은 각각 58.3%, 44.2%로 초기 병기군의 생존율이 높은 것을 알 수 있었으나 통계학적 의의는 없었다. 원격전이의 경우 17예(31.5%)에서 발생 하였으며 골, 폐, 뇌, 척추, 간 등의 순서였다. 원발 부위의 완전관해 후 6예(11.1%)에서 국소 재발하여 다시 방사선치료를 시행하거나 항암화학요법과 방사선치료를 병행하여 치료하였다. 이상의 결과에서 보면 비인강암은 50대의남자에서 호발하며, 국내에서는 WHO 제 3형이 가장 빈발한다는 것을 알 수 있었다. 또한 TNM 병기는 비인강암의 생존율을 예측하는데 적절하지 않다고 생각되며, 생존율과 연관된 인자를 찾기 위해 향후 더 많은 연구가 시행되어야 하며 그에 따라 새로운 분류법이 제시되어야 할 것으로 사료된다. Background: Nasopharyngeal cancer is a rare disease with a relatively poor prognosis because it tends to be diagnosed at an advanced stage. The aim of this study was to establish the clinical characteristics of nasopharyngeal cancer. Materials and Methods: The medical records of 54 patients with nasopharyngeal cancer from January 1993 to December 2002 were reviewed retrospectively. Forty one cases were male (75.9%) and thirteen were female (24.1%). The average age was of 46.9 (range 16 to 78 years) years. The majority of patients (79.6%) were diagnosed in the advanced stage. The most common complaints were a neck mass (55.5%) and the WHO type 11I (53.7%) was the most frequent histological type. Results: The cumulative survival rate for a 5-year period was 46.5% and the T stage, N stage, pathologic type, and clinical stage were not significantly related to the survival rate. Sixteen of 54 (31.5%) cases presented with a distant metastasis of the bone, lung, brain, spine, and liver, and six cases (11.1%) presented with a locoregional recurrence. Conclusion: TNM staging is not appropriate for predicting survival rate of nasopharyngeal carcinoma patients. Therefore, a newer staging system, which includes new factors, is needed to predict the prognosis.

호흡기상피세포에서 Leukotriene 수용체를 통한 MUC2/5AC 점액유전자의 발현과 점액분비

김용대,이재억,배창훈,서영중,예상백,송시연 대한이비인후과학회 2004 대한이비인후과학회지 두경부외과학 Vol.47 No.11