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- 저자 : 양득석 ( Sang Jik Lee (검색결과 4 건)
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양득석,정희만,이재양,이상직,Yang, Deuk-Seok,Jung, Hoe-Man,Lee, Jae-Yang,Lee, Sang-Jik 생화학분자생물학회 1988 한국생화학회지 Vol.21 No.1
소 심장 미토콘드리아에 의한 NADH 산화에 여러 약용식물 (인삼, 도라지, 더덕)의 사포닌 및 전형적인 계면 활성제 (sodium dodecyl sulfate, deoxycholate, Triton X-100)들이 미치는 영향을 조사하였다. 인삼사포닌, 도라지사포닌, 더덕사포닌 및 여러 계면 활성제들이 $10^{-5}-10^{-5}M$ 농도에서는 미토콘드리아의 NADH 산화를 촉진시킨 반면 $10^{-4}M$에서는 이를 저해하였다. 호흡활성을 저해하는 사포닌 및 계면 활성제들의 농도로 미토콘드리아를 전처리 한 후 cytochrome c를 가했을때는 다시 NADH 산화속도가 증가되었다. Cytochrome oxidase의 활성에 있어서도 위의 사포닌들과 계면 활성제들의 영향을 받았으나 미토콘드리아의 경우와는 다른 경향을 보여주었다. 사포닌 및 전형적인 계면 활성제에 의한 전자전달계의 활성이 촉진되는 것은 미토콘드리아 내 외막간에 존재하는 cytochrome c의 유동성의 증가로, 활성이 저해되는 것은 막간 cytochrome c의 유실로 추측할 수 있다. 따라서 이러한 cytochrome c의 유동성 증가 및 제거는 인상을 포함한 여러 식물 사포닌들의 계면 활성적 작용에 기인함을 알았다. The bovine heart mitochondria were separated by centrifugation and the saponins of the herbs ginseng, bellflower, and Codonopsis lanceolate were fractionated by a solvent partition. NADH oxidations were assayed in terms of 340 nm absorption decrease when the electron transport system contained the saponins. In this assay some typical surfactants dodecyl sulfate, deoxycholate, and Triton X-100 were also used for comparison. In general, the electron transport from NADH to $O_2$ was improved when $10^{-7}-10^{-5}M$ saponins or surfactants were added to the reaction systems. However. gradual decrease in the mitochondrial activity was observed as the concentration of the saponins as well as the typical surfactants was increased up to $10^{-4}M$ for example. In the cases of the pretreatment of the mitochondria with the saponins or surfactants, the oxidation rates were decreased. Increase in concentrations of exogenous cytochrome c contributed proportionally to the rate increase. The activity of a purified cytochrome oxidase was considered to be somewhat affected by the saponins or the surfactants. It is considered that the activation of the electron transport by the saponins may be caused by an increase in mobility or fluidity of the cytochrome. while the deactivation of the transport may be by an elimination of the cytochrome. Added NADH. cytochrome, and the saponins/typical surfactants may contact with the inner membrane of the mitochondrion. These mobility increase and elimination of the cytochrome may largely be attributed to the surface activity of the saponins from the plants including the ginseng.
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양득석,정회만,이재양,이상직 ( Deuk Seok Yang,Hoe Man Jung,Jae - Yang Lee,Sang Jik Lee ) 생화학분자생물학회 1988 BMB Reports Vol.21 No.1
The bovine heart mitochondria were separated by centrifugation and the saponins of the herbs ginseng, bellflower, and Codonopsis lanceolate were fractionated by a solvent partition. NADH oxidations were assayed in terms of 340 nm absorption decrease when the electron transport system contained the saponins. In this assay some typical surfactants dodecyl sulfate, deoxycholate, and Triton X-100 were also used for comparison. In general, the electron transport from NADH to O₂ was improved when 10^(-7)-10^(-5) M saponins or surfactants were added to the reaction systems. However, gradual decrease in the mitochondrial activity was observed as the concentration of the saponins as well as the typical surfactants was increased up to 10^(-4) M for example. In the cases of the pretreatment of the mitochondria with the saponins or surfactants, the oxidation rates were decreased. Increase in concentrations of exogenous cytochrome c contributed proportionally to the rate increase. The activity of a purified cytochrome oxidase was considered to be somewhat affected by the saponins or the surfactants. It is considered that the activation of the electron transport ¹y the saponins may be caused by an increase in mobility or fluidity of the cytochrome, while the deactivation of the transport may be by an elimination of the cytochrome. Added NADH, cytochrome, and the saponins/typical surfactants may contact with the inner membrane of the mitochondrion. These mobility increase and elimination of the cytochrome may largely be attributed to the surface activity of the saponins from the plants including the ginseng.
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이상직,박영수,이재양,이상호,양득석,Lee, Sang-Jik,Park, Young-Soo,Lee, Jae-Yang,Lee, Sang-Ho,Yang, Deuk-Seok 생화학분자생물학회 1991 한국생화학회지 Vol.24 No.1
소 심장 미토콘드리아 외막에 존재하는 전자운반체 cytochrome $b_5$의 추출에 미치는 영향을 계면활성제인 digitionin과 DMS(n-dodecyl-$\beta$-D-maltoside)에 대하여 조사하였고, 계면활성이 있는 인삼사포닌의 영향도 관찰하였다. Digitonin과 DMS를 미토콘드리아 외막에 각각 1% 처리하여 원심분리로 얻어진 상층액 속에 cytochrome $b_5$ 성분이 상당량 함유되어 있음을 확인하였으나 2%와 5%의 인삼사포닌을 처리했을 때는 두 경우 모두 그의 가용화를 위한 영향력을 확인할 수 없었다. Digtionin과 DMS 각 1% 처리로 얻어진, cytochrome $b_5$가 상당량 포함된, 원심분리 상층액에 대하여 DEAE-cellulose 이온교환크로마토그라피를 하였다. 용리된 cytochrome $b_5$의 순도를 전기영동에 의하여 단일 띠로 확인하였고 분자량은 13000~20000 정도이었다. The Effect of ginseng saponin on the extraction of cytochrome $b_5$ which is an electron-transport protein present at the outer membrane of bovine heart mitochondrion was compared with those of two surfactants-digitonin and DMS (n-dodecyl-$\beta$-D-maltoside). It was observed that much cytochrome $b_5$ was contained in the supernatant obtained by the spinning the mitochondrial outer membrane which was treated with 1% of digitonin or DMS. But, treating the membrane with 2% or 5% of ginseng saponin could not solubilize the cytochrome. An ion-exchange chromatography of DEAF-cellulose was performed for the supernatant rich in cytochrome $b_5$ which was obtained from the treatment of 1% of digitonin or DMS and then spinning. The purity of the cytochrome fraction eluted was confirmed through a single electrophoretic band. The molecular weight of the protein was 13,000-20,000.
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이상직,박영수,이재양,이상호,양득석 ( Sang Jik Lee,Young Soo Park,Jae Yang Lee,Sang Ho Lee,Deuk Seok Yang ) 생화학분자생물학회 1991 BMB Reports Vol.24 No.1
The Effect of ginseng saponin on the extraction of cytochrome b_5 which is an electrontransport protein present at the outer membrane of bovine heart mitochondrion was compared with those of two surfactants-digitonin and DMS (n-dodecyl-β-D-maltoside). It was observed that much cytochrome bs was contained in the supernatant obtained by the spinning the mitochondrial outer membrane which was treated with 1% of digitonin or DMS. But, treating the membrane with 2% or 5% of ginseng saponin could not solubilize the cytochrome. An ion-exchange chromatography of DEAF-cellulose was performed for the supernatant rich in cytochrome b_5 which was obtained from the treatment of 1% of digitonin or DMS and then spinning. The purity of the cytochrome fraction eluted was confirmed through a single electrophoretic band. The molecular weight of the protein was 13,000-20,000.
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