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안해천,최은옥 한국식품과학회 2015 Food Science and Biotechnology Vol.24 No.4
Chlorophyll, carotenoid, total phenolic compound, and tocopherol contents of daraesoon (shoot of the Siberian gooseberry tree, Actinidia arguta Planchon) were determined after blanching and during drying in the dark (13 days) and under light (10 days), using HPLC and spectrophotometry. Blanching caused a significant (p<0.05) increase in the total chlorophyll content to 4,629.8 mg/kg from 2,932.5 mg/kg, and in the β-carotene content to 4,953.4 mg/kg from 4,590.5 mg/kg, compared with controls. Drying of blanched daraesoon caused a significant (p<0.05) loss of chlorophylls and carotenoids, and carotenoids were more affected by light than chlorophylls. Total phenolic compounds and tocopherols were also degraded during blanching and drying, with more degradation of tocopherols (up to 54.3%) than for total phenolic compounds (up to 15.8%). In spite of degradation of pigments and antioxidants during drying, dried daraesoon provides high levels of health-beneficial carotenoids, total phenolic compounds, and tocopherols.
안해천,김정하,김정인,어중혁,최은옥 한국식품과학회 2014 Food Science and Biotechnology Vol.23 No.4
In vitro α-glucosidase and pancreatic lipaseinhibitory activities of ethanol extracts of samnamul (driedyoung shoots of Aruncus dioicus) during rehydration(soaking, 30 min boiling, and re-soaking in water) andsubsequent cooking at 180oC with/without perilla oil weredetermined for evaluation of its anti-diabetic and antiobesitypotential. Polyphenol and flavonoid contents weremonitored by spectrophotometry. Rehydration and boilingof samnamul reduced α-glucosidase and pancreatic lipaseinhibitory activities as well as polyphenol and flavonoidcontents, whereas soaking time did not have a significanteffect. Of the rehydration and cooking processes, 30 minboiling was the most critical in reducing anti-diabetic andanti-obesity activities as well as antioxidant contents. Perillaoil addition improved α-glucosidase inhibitory activity,possibly through polyphenol addition. These results suggestthat dietary intake of samnamul can reduce the risks ofdiabetes and obesity, and perilla oil can additionallyimprove its health benefits.
건조 삼나물과 다래순의 재수화와 고온 가열조리 중 색소와 산화방지성분의 함량 변화
안해천,최은옥,An, Haechun,Choe, Eunok 한국식품조리과학회 2016 한국식품조리과학회지 Vol.32 No.4
Purpose: This study was conducted to evaluate the effect of rehydration and subsequent heating at high temperature on the pigments and antioxidants of dried samnamul (Aruncus dioicus) and daraesoon (Actinidia arguta). Methods: Rehydration included 16 h-soaking in cold water, and 30 min-boiling and 1 h-infusion in water. Rehydrated samnamul and daraesoon were heated at $180^{\circ}C$ for 10 or 20 min with or without perilla oil addition (10%) for cooking. Pigments and antioxidants were determined by HPLC and spectrophotometry. Results: Rehydration caused decreases in pigment and polyphenol contents, but increase in tocopherol content. Cooking by heating without addition of perilla oil resulted in increases in chlorophyll and carotenoid contents, but decreases in polyphenol and tocopherol contents. Decrease in tocopherol content by heating at $180^{\circ}C$ was reversed by the addition of perilla oil. Conclusion: This study strongly suggested that cooking of samnamul and daraesoon at $180^{\circ}C$ with perilla oil could improve color, texture, and potential health functionality by recovering the loss of antioxidants and pigments with antioxidant activity.
우리나라 일부 산채의 in vitro 산화 방지 활성, 알파-글루코시데이스와 췌장 라이페이스 저해 활성
안해천(Haecheon Ahn),정라나(Lana Chung),최은옥(Eunok Choe) 한국식품과학회 2015 한국식품과학회지 Vol.47 No.2
묵나물 형태의 삼나물, 미역취, 다래순, 방풍나물, 생채인 참나물의 75% 에탄올 추출물의 산화 방지 활성, α-glucosidase와 pancreatic lipase 저해 활성을 평가하고 폴리페놀 화합물, 플라보노이드, 토코페롤 함량을 구하여 이들 활성과의 상관관계를 분석하였다. DPPH 라디칼 소거활성은 삼나물과 다래순에서, α-glucosidase 저해활성은 삼나물, 다래순, 방풍나물에서, pancreatic lipase 저해 활성은 미역취와 다래순에서 높았다. 삼나물 추출물은 폴리페놀 화합물과 플라보노이드 함량이 매우 높았으며, 다래순 추출물은 다른 산채에 비해 토코페롤 함량이 높았다. 산채 추출물의 산화 방지 성분 함량과 in vitro 활성 사이의 상관관계는 플라보노이드와 DPPH 라디칼 소거 활성 사이의 상관관계(r2=0.8895)를 제외하고는 높지 않았으나, α-glucosidase와 pancreatic lipase 저해 활성이 높았던 삼나물과 다래순은 항당뇨, 항비만 활성을 가진 잠재적인 식품 자원으로 기대되었다. This study evaluated in vitro antioxidant activity, antioxidant content, and α-glucosidase and pancreatic lipase inhibitory activities of ethanol extracts of samnamul (shoot of Aruncus dioicus), miyeokchwi (Solidago virgaurea), daraesoon (shoot of Actinidia arguta Planchon), and bangpungnamul (leaves of Ledebouriella seseloides), as muknamul, and fresh chamnamul (Pimpinella brachycarpa). Tocopherol content (4.8-78.3 mg/100 g) of sanchae was lower than polyphenols (4.4-12.2 g/100 g). Daraesoon with high tocopherol contents showed high antioxidant activity and α-glucosidase and pancreatic lipase inhibitory activities. Samnamul had the highest levels of polyphenols and flavonoids, the highest antioxidant activity, and α-glucosidase inhibition. Antioxidant activity was correlated with flavonoid content (r2=0.8895), but was not correlated with the levels of other antioxidants tested, suggesting that polyphenol content in samnamul, miyeokchwi, daraesoon, bangpung, and chamnamul might not be critical determinant of antioxidant activity. Our results strongly suggest that samnamul and daraesoon could be useful in the treatment of diabetes and obesity.
Effects of Light and Lipids on Chlorophyll Degradation
Edwald Lee,안해천,최은옥 한국식품과학회 2014 Food Science and Biotechnology Vol.23 No.4
Stability of chlorophylls was studied in a systemof paraffin oil with added oleic acid or triolein under 1,700lx and in the dark. Chlorophyll contents were determinedusing HPLC. Sample oxidation was evaluated using theheadspace oxygen content based on GC and the peroxidevalue. The chlorophyll content decreased with time andwas higher and decreased faster under light than in thedark. Singlet oxygen was involved in chlorophyll degradationunder light. Chlorophyll degradation was lower and slowerin samples with added lipids than in samples withoutlipids. Protection of chlorophyll from photodegradationwas greater using triolein than using oleic acid. Oxidationwas slightly higher in samples with added lipids than insamples without lipids, however, there was no significant(p>0.05) difference between samples with added oleic acidor triolein. Decreased chlorophyll degradation under lightby lipids is due to competition between lipids andchlorophyll for singlet oxygen.