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가시오갈피 생물반응기 배양에서 질소급원이 세포생장과 이차대사 생산에 미치는 영향
안진권,이위영,박소영,Ahn, Jin-Kwon,Lee, Wi-Young,Park, So-Young 한국식물생명공학회 2003 식물생명공학회지 Vol.30 No.3
The effects of inorganic nitrogen sources such as KNO$_3$ and NH$_4$ NO$_3$ on cell growth and production of chlorogenic acid and eleutheroside E derivative were investigated in 5L bioreactor cultures of Eleutherococcus senticosus. The cell growth in the 1/2MS medium containing 15mMKNO$_{3}$. The fresh weight of cells harvested from bioreactor was affected by the concentration ratio of NO$_3$$^{[-10]}$ and NH$_4$$^{+}$ in culture medium. At the viewpoint of secondary metabolite production, the production of chlorogenic acid was affected by the concentration of NH$_4$$^{+}$ in the culture medium, but not by the total concentration of nitrogen sources in the culture medium. Futhermore, eleutheroside E derivative production was also affected by the concentration ratio of NO$_3$$^{[-10]}$ and NH$_4$$^{+}$ in the culture medium. Base on those results, it is suggested that cell growth and production of secondary metabolite(chlorogenic acid and eleutheroside E derivative) could be manipulated by controlling the total concentration of nitrogen sources and the concentration ratio of NO$_3$$^{[-10]}$ and NH$_4$$^{+}$ in the culture medium. medium.
안진권,박영기,이위영,박소영 한국산림과학회 2007 한국산림과학회지 Vol.96 No.5
This study was caried out to investigate the impacts of jasmonic acid (JA) on adventitious rootculture of Eleutherococcus senticosus. Adventitious root of E. senticosus were treated with jasmonic acid(JA) and cultured for 30 days. JA inhibited the root growth but increased eleutherosides accumulation, totaltotal phenolic contents in E. senticosus adventitious root to 39.81 g/g, about 2.6 times higher than that ofthe control. Consequently, high acumulation of total phenolic contents led to increase the antioxidantactivity to 82.41%. The antioxidant activity of control was 37.89% at 250 g/mL. A linear correlation (R2= 0.9937, 0.9648 and 0.983) was also shown between antioxidant activity (at 1250, 1875, and 2500 g/E. senticosus.
생물반응기 배양에서 생장조절제에 따른 섬오갈피 부정근 증식 및 Eleutheroside와 Chlorogenic Acid생산
안진권,박소영,이위영,이정주 한국식물생명공학회 2005 JOURNAL OF PLANT BIOTECHNOLOGY Vol.32 No.1
The influence of different growth regulators on biomass of adventitious roots and secondary metabolites accumulation in bioreactor cultures of Eleutherococcus koreanum was studied. The maximum growth of adventitious roots was observed in the presence of 2.0 mg/L IBA (6.7 g DW/L). However the productivity of eleutheroside E was not significantly different among IBA levels. High level of thidiazuron (TDZ) efficiently increased both root growth and secondary metabolites production. Especially when 0.1 mg/L TDZ was combined with 3.0 mg/L IBA, the highest eleutheroside E accumulation (3,327 g/g DW) was observed. When the same TDZ level was treated in combination with 5.0 mg/L IBA, both chlorogrnic acid and eleutheroside B were accumulated to maximum level. In contrast, zeatin remarkably suppressed the accumulation of both eleutheroside B and chlorogenic acid. 생물반응기를 이용한 섬오갈피 부정근 배양시 첨가되는 생장조절제의 종류와 농도에 따른 부정근의 생장과 이차대사산물 생산과의 관계를 조사하였다. IBA 농도별 부정근 생장은 IBA 2.0 mg/L에서 6.7 g DW/L로 가장 높았으나, eleutheroside E 함량은 IBA 농도에 따라 차이가 없었다. TDZ 농도에 따른 부정근 생장은 농도가 증가함에 따라 생장도 증가하여 eleutheroside E 함량은 IBA 3.0 mg/L에 TDZ 0.1 mg/L 처리구에서 3,327 μg/g DW로 가장 생산량이 많았다. 이와 달리 eleutheroside B (153 μg/g DW)과 chlorogenic acid (7.7 mg/g DW)는 IBA 5.0 mg/L에 TDZ 0.1 mg/L 처리구에서 함량이 가장 높았다. Zeatin은 부정근 생육과 eleutheroside E 함량에는 크게 영향을 미치지 않으나 eleutheroside B와 chlorogenic acid 생산을 크게 억제하였다.
안진권,이위영,박영기,Ahn, Jin-Kwon,Lee, Wi-Young,Park, Young-Ki 한국산림과학회 2007 한국산림과학회지 Vol.96 No.1
This study was carried out to investigate the effect of $NO_3{^-}$ and $NH_4{^+}$ on the adventitious root growth and eleuthroside synthesis of Eleutherococcus senticosus during 3 L-bioreactor culture. The change of medium component ratio was also measured during culture. The fresh weignt of adventitious root reached to the greatest level of 24.4g FW/L in the presence of 50 mM $NO_3{^-}$ and 10 mM $NH_4{^+}$, representing 3.4-fold increase compared to the 60 mM $NH_4{^+}$. However, as the increase of the portion of $NH_4{^+}$, the root growth was decreased. Maximum eleutheroside B and E1 production were $249{\mu}g/g$ and $43{\mu}g/g$, respectively, with 30 mM total nitrogen source. The maximum production of eleutheroside E were $788{\mu}g/g$ with 120 mM total nitrogen source. The greatest weight of adventitious root increased up to 6.2 fold of inoculum size within 9 weeks. The change of pH was influenced from 4.81 to 6.35 and the amounts of $NH_4{^+}$ and $K^+$ were decreased during culture periods. From these results we suggest, need further study of various treatment to increase the growth of biomass and the accumulation of useful secondary metabilites. 생물반응기를 이용하여 가시오갈피 부정근 증식시 부정근의 생장과 유용 이차대사산물인 eleuthroside 류 생산에 미치는 $NO_3{^-}$와 $NH_4{^+}$의 영향 및 배양기간에 따른 배지성분의 변화량을 분석하였다. 부정근 증식은 질소농도가 50 mM $NO_3{^-}$와 10 mM $NH_4{^+}$ 농도비율로 참가된 처리구에서 최대(24.4g FW/L)에 이르러 60 mM $NH_4{^+}$ 첨가에 비해서는 3.4배 증식되었다. 또한 $NH_4{^+}$의 첨가농도비율이 높아질수록 부정근의 증식은 감소하였다. Eleutheroside B와 E1은 총 질소량이 30 mM 처리에서 각각 $249{\mu}g/g$와 $43{\mu}g/g$을 생산하여 가장 우수하였으나, eleutheroside E는 총 질소농도가 높아질수록 생산량도 높아져 120 mM에서 $788{\mu}g/g$로 가장 우수하였다. 부정근 생장은 초기 접종량 대비 배양 9주만에 6.2배가 증식되었고, 배양기간별 배지 성분의 변화에서는 산도는 4.81-6.35로 변화가 비교적 심했고, $NH_4{^+}$와 $K^+$는 배양기간이 경과할수록 함유량이 낮았다. 이러한 결과로 보아 부정근의 생장과 유용이차대사산물의 함량을 높이기 위하여 다양한 처리의 연구가 수행되어야 할 것으로 생각된다.
생물반응기 배양에서 생장조절제에 따른 섬오갈피 부정근 증식 및 Eleutheroside와 Chlorogenic Acid 생산
안진권,박소영,이위영,이정주,Ahn Jin-Kwon,Park So-Young,Lee Wi-Young,Lee Jeong-Ju 한국식물생명공학회 2005 식물생명공학회지 Vol.32 No.1
생물반응기를 이용한 섬오갈피 부정근 배양시 첨가되는 생장조절제의 종류와 농도에 따른 부정근의 생장과 이차대사산물 생산과의 관계를 조사하였다. IBA 농도별 부정근 생장은 IBA $2.0\;\cal{mg/L}$에서 6.7 g DW/L로 가장 높았으나, eleutheroside E 함량은 IBA 농도에 따라 차이가 없었다 TDZ 농도에 따른 부정근 생장은 농도가 증가함에 따라 생장도 증가하여 eleutheroside E 함량은 IBA $3.0\;\cal{mg/L}$에 TDZ $0.1\;\cal{mg/L}$ 처리구에서 $3,327\;{\mu}g/g$ DW로 가장 생산량이 많았다. 이와 달리 eleutheroside B ($153\;{\mu}g/g$ DW)과 chlorogenic acid ($7.7\;\cal{mg/g}$ DW)는 IBA $5.0\;\cal{mg/L}$에 TDZ $0.1\;\cal{mg/L}$ 처리구에서 함량이 가장 높았다. Zeatin은 부정근 생육과 eleutheroside E 함량에는 크게 영향을 미치지 않으나 eleuthe-roside B와 chlorogenic acid 생산을 크게 억제하였다. The influence of different growth regulators on biomass of adventitious roots and secondary metabolites accumulation in bioreactor cultures of Eleutherococcus koreanum was studied. The maximum growth of adventitious roots was observed in the presence of $2.0\;\cal{mg/L}$ IBA (6.7 g DW/L). However the productivity of eleutheroside E was not significantly different among IBA levels. High level of thidiazuron (TDZ) efficiently increased both root growth and secondary metabolites production. Especially when $0.1\;\cal{mg/L}$ TDZ was combined with $3.0\;\cal{mg/L}$ IBA, the highest eleutheroside E accumulation ($3,327{\mu}g/g $DW) was observed. When the same TDZ level was treated in combination with $5.0\;\cal{mg/L}$ IBA, both chlorogrnic acid and eleutheroside B were accumulated to maximum level. In contrast, zeatin remarkably suppressed the accumulation of both eleutheroside B and chlorogenic acid.