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해조류 중의 Anti - Tumor Initiator 및 Promoter의 해석 - 4 : 발암성 Heterocyclic Amine에 대한 곰피 추출물 중의 돌연변이원성 억제인자
박영범(Yeung-Beom Park),안종관(Jong-Khan Ahn),유승재(Sung-Jae Yoo),박덕천(Douck-Choun Park),김인수(In-Soo Kim),박영호(Yeung-Ho Park),김선봉(Seon-Bong Kim) 한국식품영양과학회 1998 한국식품영양과학회지 Vol.27 No.3
해조류 중의 돌연변이원성 억제인자를 해석하기 위하여 돌연변이원성 억제작용이 강한 곰피를 시료로 하여 식품의 가열조리시에 생성되는 발암성 heterocyclic amine인 PhIP 및 MeIQx에 대한 돌연변이원성 억제효과와 억제 인자를 살펴 보았다. 실험의 결과, 곰피로부터 추출한 알긴산의 돌연변이원성 억제효과는 미미하였으며, 그 가수분해물은 10㎎/plate의 농도에서 PhIP에 대하여는 41.0~51.6%, MeIQx에 대하여는 24.3~30.5%의 억제효과를 나타내었고, 가수분해시간에 따른 돌연변이원성 억제작용의 영향은 적었다. 곰피의 phenol추출물과 bromophenol은 0.5㎎/plate에서 PhIP 및 MeIQx에 대하여 90% 정도의 돌연변이원성 억제효과를 나타내었다. 그리고, 곰피 색소 중의 chlorophyll 및 그 유도체 중에서는 chlorophyllin이 억제작용이 높게 나타나 PhIP 및 MeIQx에 대하여 각각 90% 및 80% 이상의 억제효과를 나타내었다. 곰피의 carotenoid 성분중에서는 lutein 및 α-cryptoxanthin이 PhIP 및 MeIQx에 대해 약 90%/㎎의 억제효과를 나타내었다. The present study was performed to elucidate desmutagenic principles from Ecklonia stolonifera extracts against 2-amino-l-methyl-6-phenylimidazo[4,5-b]pyridine(PhIP) and 2-amino-3,8-dimethylimidazo[4,5-f]quinoxaline{MeIQx) with Salmonella/mammalian-microsome mutagenicity test. Alginate, phenols, chlorophyll and carotenoids from Ecklonia stolonifera were extracted and their desmutagenicities were assayed. Alginate hydrolysates showed desmutagenic activities against PhIP and MeIQx at high level dose. Phenol fractions and bromophenol showed desmutagenic activity of about 90% per 0.5㎎ against PhIP and MeIQx. Chlorophyllin among chlorophyll derivatives exhibited remarkable desmutagenic activities of 92.9% and 82.7% at 20μM against PhIP and MeIQx, respectively. Carotenoids, such as lutein and α-cryptoxanthin isolated from Ecklonia stolonifera exerted also high desmutagenic activity. Major desmutagenic substances from Ecklonia stolonifera are considered to be chlorophyllin, phenols, lutein, α-cryptoxanthin and low molecular alginates.
해조류 중의 anti - tumor initiator 및 promoter 의 해석 - 곰피 추출물중의 발암 promotion 억제 인자
박영범(Yeung Beom Park),김인수(In Soo Kim),유승재(Sung Jae Yoo),안종관(Jong Khan Ahn),이태기(Tae Gee Lee),박덕천(Douck Chon Park),김선봉(Seon Bong Kim) 한국수산과학회 1998 한국수산과학회지 Vol.31 No.4
To elucidate anti-tumor promoter from seaweed. the anti-tumor promoting activity of Ecklonia stolonifera, Undaria pinnatifida and Laminaria japonica extracts were determined by Epstein-Barr virus (EBV)-early antigen (EA) induction caused by a tumor promoter, teleocidin B-4. The methanol extracts of seaweed were subsequently fractionated with diethyl ether, distilled water, chloroform and ethyl acetate. Among the solvent fractions tested, chloroform and ethyl acetate fraction of E. stolonifera showed a high anti-tumor promoting activity at the levels of 88.0 and 85.9% by the addition of 20 ㎍/㎖respectively. To characterize anti-tumor promoters from solvent fractions of E. stolonifera, the effects of phenols, chlorophyll derivatives and carotenoids on the anti-tumor promoting activity were investigated. Phenols, such as bromophenol and phloroglucinol showed anti-tumor promoting activity of 57∼66% at 20 ㎍/㎖. Pigments, such as chlorophylls and carotenoids exerted high anti·tumor promoting activities. Chlorophyll a and pheophorbide a exhibited the activity of 77.4% and 66.6% at 5μM/㎖respectively. The active compounds of carotenoids were tentatively identified as lutein and α-cryptoxanthin from the profiles of visible spectra and R_f value of their authentic compounds, and showed anti-tumor promoting activities of 76.9% and 84.4% at dose of 20 ㎍/㎖, respectively.
해조류 중의 anti-tumor initiator 및 promoer 의 해석 - PhIP 와 MeIQx 의 돌연변이원성을 억제하는 해조 추출물 소재의 연구
박영범(Yeung Beom Park),김인수(In Soo Kim),유승재(Sung Jae Yoo),안종관(Jong Khan Ahn),이태기(Tae Gee Lee),박덕천(Douck Chon Park),김선봉(Seon Bong Kim) 한국수산과학회 1998 한국수산과학회지 Vol.31 No.4
The present study was conducted to prepare seaweed extracts suppressing mutagenic activity of 2-amino-1methyl-6-phenylimidazo[4,5-b]pyridine(PhIP) and 2-amino-3,8-dimethylimidazo[4,5-f]quinoxaline(MeIQx) derived from cooked meat products. The tumor initiation activity of PhIP and MeIQx was assayed with Ames method using Salmonella typhimurium TA98 in the presence of S-9 mixtures before and after addition of methanol-solubles of seaweed, such as, Phaeophyta; Undaria pinnatifida, Ecklonia stolonifera, Ecklonia stolonifera, Ecklonia cava, Laminaria japonica, Sargassum fulvellum, Sargassum horneri, Sargassum miyabei, Sargassu, thunbergii, Agarum cribrosum and Hizikia fusiformis, Phodophyta; Porphyra yezoensis, Grateloupia elliptica, Lomentaria catenata, Plocamium telfairiae and Gracilaria verrucosa, the highter desmutagenic activity than Rhodophyta and Chlorophyta. E. stolonifera, E. cava and S. miyabei, among Phaeophyta exerted the stronger desmutagenic activity(above 90%/2 ㎎). The ethyl actate, diethyl ether and chloroform extracts except water extracts from E, stolonifera exhibited a high desmutagenic activity. The ethyl acetate extract of E. stolonifera which showed highest acuity was fractionated with SephadexLH20 column chromatography to give active fraction A-7, which showed desmutagenicity of 90%/㎎ against PhIP and 80%/㎎ against MeIQx. The active fraction had the absorbance at 207.7 and 232㎚.