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Aroase AP10에 의한 문어 가수분해물의 Angiotensin Converting Enzyme 저해 Peptide의 특성
박영범(Yeung Beom Park) 한국식품영양과학회 2009 한국식품영양과학회지 Vol.38 No.2
문어육의 Aroase AP10 가수분해물을 제조하고 이들 가수분해물을 한외여과막을 통과시켜 회수한 분자량 10,000 Da이하의 저분자물질을 Bio-gel P-2 gel chromatography를 행하여 ACE 저해효과를 가지는 3개 획분을 분취하였다. 또한 이들 획분을 SuperQ-Toyopearl 650S column을 이용한 이온교환크로마토그래피에 의해 4개의 활성획분을 분리하였다. 이중 ACE 저해효과가 가장 높은 C-1 획분의 아미노산 조성은 arginine, lysine, histidine 및 leucine의 함량이 가장 많아 전체의 약 60%를 차지하였으며 IC??은 3.10 ㎍으로 나타났다. The peptides from Aroase AP10 enzymatic hydrolysates of octopus proteins were isolated and tested for inhibitory activity against angiotensin converting enzyme (ACE). The Aroase AP10 hydrolysates were filtered through PM-10 membrane (M.W. cut-off 10,000) to obtain the peptides fractions with ACE inhibition activity. These fractions were applied to a Biogel P-2 column. Three active fractions (A, B, and C) were collected and applied to a SuperQ-Toyopearl 650S column chromatography, leading to the isolation of four active fractions (A-1, A-2, B-1, and C-1). Among the active fractions, C-1 had the highest ACE inhibitory activity (IC??=3.10 ㎍). The main composition of its amino acids is arginine, lysine, histidine and leucine, which cover about 60% of the total amino acids.
해조류 중의 Anti - Tumor Initiator 및 Promoter의 해석 - 4 : 발암성 Heterocyclic Amine에 대한 곰피 추출물 중의 돌연변이원성 억제인자
박영범(Yeung-Beom Park),안종관(Jong-Khan Ahn),유승재(Sung-Jae Yoo),박덕천(Douck-Choun Park),김인수(In-Soo Kim),박영호(Yeung-Ho Park),김선봉(Seon-Bong Kim) 한국식품영양과학회 1998 한국식품영양과학회지 Vol.27 No.3
해조류 중의 돌연변이원성 억제인자를 해석하기 위하여 돌연변이원성 억제작용이 강한 곰피를 시료로 하여 식품의 가열조리시에 생성되는 발암성 heterocyclic amine인 PhIP 및 MeIQx에 대한 돌연변이원성 억제효과와 억제 인자를 살펴 보았다. 실험의 결과, 곰피로부터 추출한 알긴산의 돌연변이원성 억제효과는 미미하였으며, 그 가수분해물은 10㎎/plate의 농도에서 PhIP에 대하여는 41.0~51.6%, MeIQx에 대하여는 24.3~30.5%의 억제효과를 나타내었고, 가수분해시간에 따른 돌연변이원성 억제작용의 영향은 적었다. 곰피의 phenol추출물과 bromophenol은 0.5㎎/plate에서 PhIP 및 MeIQx에 대하여 90% 정도의 돌연변이원성 억제효과를 나타내었다. 그리고, 곰피 색소 중의 chlorophyll 및 그 유도체 중에서는 chlorophyllin이 억제작용이 높게 나타나 PhIP 및 MeIQx에 대하여 각각 90% 및 80% 이상의 억제효과를 나타내었다. 곰피의 carotenoid 성분중에서는 lutein 및 α-cryptoxanthin이 PhIP 및 MeIQx에 대해 약 90%/㎎의 억제효과를 나타내었다. The present study was performed to elucidate desmutagenic principles from Ecklonia stolonifera extracts against 2-amino-l-methyl-6-phenylimidazo[4,5-b]pyridine(PhIP) and 2-amino-3,8-dimethylimidazo[4,5-f]quinoxaline{MeIQx) with Salmonella/mammalian-microsome mutagenicity test. Alginate, phenols, chlorophyll and carotenoids from Ecklonia stolonifera were extracted and their desmutagenicities were assayed. Alginate hydrolysates showed desmutagenic activities against PhIP and MeIQx at high level dose. Phenol fractions and bromophenol showed desmutagenic activity of about 90% per 0.5㎎ against PhIP and MeIQx. Chlorophyllin among chlorophyll derivatives exhibited remarkable desmutagenic activities of 92.9% and 82.7% at 20μM against PhIP and MeIQx, respectively. Carotenoids, such as lutein and α-cryptoxanthin isolated from Ecklonia stolonifera exerted also high desmutagenic activity. Major desmutagenic substances from Ecklonia stolonifera are considered to be chlorophyllin, phenols, lutein, α-cryptoxanthin and low molecular alginates.
박영범(Yeung-Beom Park) 한국식품영양과학회 2005 한국식품영양과학회지 Vol.34 No.8
일상 식생활에서 널리 섭취하고 있는 식용 및 미식용인 16종의 해조류를 사용하여 니트로사민의 직접적인 생성인자인 아질산염 소거작용에 관하여 검토하였다. 그 결과, 갈조류의 경우가 녹조류 및 홍조류에 비해 우수한 분해효과를 나타내었으며 갈조류 중에서도 다시마과의 감태, 곰피, 구멍쇠 미역이 뛰어난 아질산염 분해효과를 나타내었다. 또한 각 시료 모두 pH의존성이 크게 나타나 pH 4.2 및 6.0에서보다 pH 1.2에서 아질산염 분해효과가 뛰어났으며 아질산염 분해효과가 우수할수록 환원력이 높아 아질산염 분해능과 환원력은 밀접한 관련성을 나타내었다. Nitrite plays an important role in the formation of carcinogenic nitrosamine. In order to effectively inhibit the formation of carcinogenic nitrosamine in food and biological systems, scavenging of residual nitrite is necessary. In screening test of nitrite-scavenging effect of seaweed, the methanol extract from Phaeophyta was more effective than those from Rhodophyta and Chlorophyta. Among the brown algae family, Laminariaceae, A. cribrosum (90.4%) and E. cava (80.1%), E. stolonifera (79.0%) that belong to genus Ecklonia showed marked nitrite-scavenging effect at pH 1.2. Furthermore, these algae showed potent reducing power and radical scavenging activity. The nitrite-scavenging effect was correlated with level of reducing power.
오징어 간 액젓으로부터 분리된 Angiotensin Converting Enzyme 저해 Peptide의 특성
박영범(Yeung Beom Park) 한국식품영양과학회 2010 한국식품영양과학회지 Vol.39 No.11
오징어 가공 부산물인 오징어 간의 효율적 이용을 위하여 오징어 간을 이용하여 액젓을 제조하고 이들 액젓의 ACE 저해효과를 살펴보았다. 액젓의 ACE 저해활성은 12개월까지는 점차적으로 증가하였으나 그 이후에는 저해활성이 둔화되어 거의 일정한 저해활성을 유지하였다. 숙성 액젓 중 15개월째 액젓(IC??=29.66 ㎍)을 한외여과막으로 통과시켜 회수한 분자량 10,000 Da 이하의 저분자물질을 Bio-gel P-2gel chromatography를 행하여 ACE 저해효과를 가지는 3개 획분을 분취하였다. 이들 획분 중에서 ACE 저해효과가 가장 높은 B 획분을 SuperQ-Toyopearl 650S column을 이용한 음이온 교환크로마토그래피에 의해 B-1의 활성획분을 분리하였다. 획분 B-1의 아미노산 조성은 lysine, glycine 및 proline의 함량이 가장 많아 전체의 약 85%를 차지하였으며 IC??은 5.46 ㎍으로 나타났다. In order to utilize squid liver by-products, which is normally discarded as industrial waste in the process of squid manufacturing, salt-fermented squid liver sauce was prepared experimentally and also tested for inhibitory activity against angiotensin converting enzyme (ACE). ACE inhibitory activity of squid liver sauce was increased with the elapse of fermentation days until 12 months, followed by a constant level of inhibitory activity thereafter. 15-month-old sauce (IC??=29.66 ㎍) was filtered through PM-10 membrane (M.W. cut-off 10,000 Da) to obtain the peptides fractions with ACE inhibition activity. Filtered fractions were applied to a Bio-gel P-2 column and three active fractions (A, B and C) were collected. Among them, fraction B applied to a SuperQ-Toyopearl 650S column chromatography lead to the isolation of active B-1 fraction. It has the ACE inhibitory activity (IC??=5.46 ㎍). The main composition of its amino acids is lysine, glycine and proline, which cover about 85% of the total amino acids.
해조류 중의 anti - tumor initiator 및 promoter 의 해석 - 곰피 추출물중의 발암 promotion 억제 인자
박영범(Yeung Beom Park),김인수(In Soo Kim),유승재(Sung Jae Yoo),안종관(Jong Khan Ahn),이태기(Tae Gee Lee),박덕천(Douck Chon Park),김선봉(Seon Bong Kim) 한국수산과학회 1998 한국수산과학회지 Vol.31 No.4
To elucidate anti-tumor promoter from seaweed. the anti-tumor promoting activity of Ecklonia stolonifera, Undaria pinnatifida and Laminaria japonica extracts were determined by Epstein-Barr virus (EBV)-early antigen (EA) induction caused by a tumor promoter, teleocidin B-4. The methanol extracts of seaweed were subsequently fractionated with diethyl ether, distilled water, chloroform and ethyl acetate. Among the solvent fractions tested, chloroform and ethyl acetate fraction of E. stolonifera showed a high anti-tumor promoting activity at the levels of 88.0 and 85.9% by the addition of 20 ㎍/㎖respectively. To characterize anti-tumor promoters from solvent fractions of E. stolonifera, the effects of phenols, chlorophyll derivatives and carotenoids on the anti-tumor promoting activity were investigated. Phenols, such as bromophenol and phloroglucinol showed anti-tumor promoting activity of 57∼66% at 20 ㎍/㎖. Pigments, such as chlorophylls and carotenoids exerted high anti·tumor promoting activities. Chlorophyll a and pheophorbide a exhibited the activity of 77.4% and 66.6% at 5μM/㎖respectively. The active compounds of carotenoids were tentatively identified as lutein and α-cryptoxanthin from the profiles of visible spectra and R_f value of their authentic compounds, and showed anti-tumor promoting activities of 76.9% and 84.4% at dose of 20 ㎍/㎖, respectively.
해조류 중의 anti-tumor initiator 및 promoer 의 해석 - PhIP 와 MeIQx 의 돌연변이원성을 억제하는 해조 추출물 소재의 연구
박영범(Yeung Beom Park),김인수(In Soo Kim),유승재(Sung Jae Yoo),안종관(Jong Khan Ahn),이태기(Tae Gee Lee),박덕천(Douck Chon Park),김선봉(Seon Bong Kim) 한국수산과학회 1998 한국수산과학회지 Vol.31 No.4
The present study was conducted to prepare seaweed extracts suppressing mutagenic activity of 2-amino-1methyl-6-phenylimidazo[4,5-b]pyridine(PhIP) and 2-amino-3,8-dimethylimidazo[4,5-f]quinoxaline(MeIQx) derived from cooked meat products. The tumor initiation activity of PhIP and MeIQx was assayed with Ames method using Salmonella typhimurium TA98 in the presence of S-9 mixtures before and after addition of methanol-solubles of seaweed, such as, Phaeophyta; Undaria pinnatifida, Ecklonia stolonifera, Ecklonia stolonifera, Ecklonia cava, Laminaria japonica, Sargassum fulvellum, Sargassum horneri, Sargassum miyabei, Sargassu, thunbergii, Agarum cribrosum and Hizikia fusiformis, Phodophyta; Porphyra yezoensis, Grateloupia elliptica, Lomentaria catenata, Plocamium telfairiae and Gracilaria verrucosa, the highter desmutagenic activity than Rhodophyta and Chlorophyta. E. stolonifera, E. cava and S. miyabei, among Phaeophyta exerted the stronger desmutagenic activity(above 90%/2 ㎎). The ethyl actate, diethyl ether and chloroform extracts except water extracts from E, stolonifera exhibited a high desmutagenic activity. The ethyl acetate extract of E. stolonifera which showed highest acuity was fractionated with SephadexLH20 column chromatography to give active fraction A-7, which showed desmutagenicity of 90%/㎎ against PhIP and 80%/㎎ against MeIQx. The active fraction had the absorbance at 207.7 and 232㎚.