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해조류 중의 anti-tumor initiator 및 promoer 의 해석 - PhIP 와 MeIQx 의 돌연변이원성을 억제하는 해조 추출물 소재의 연구
박영범(Yeung Beom Park),김인수(In Soo Kim),유승재(Sung Jae Yoo),안종관(Jong Khan Ahn),이태기(Tae Gee Lee),박덕천(Douck Chon Park),김선봉(Seon Bong Kim) 한국수산과학회 1998 한국수산과학회지 Vol.31 No.4
The present study was conducted to prepare seaweed extracts suppressing mutagenic activity of 2-amino-1methyl-6-phenylimidazo[4,5-b]pyridine(PhIP) and 2-amino-3,8-dimethylimidazo[4,5-f]quinoxaline(MeIQx) derived from cooked meat products. The tumor initiation activity of PhIP and MeIQx was assayed with Ames method using Salmonella typhimurium TA98 in the presence of S-9 mixtures before and after addition of methanol-solubles of seaweed, such as, Phaeophyta; Undaria pinnatifida, Ecklonia stolonifera, Ecklonia stolonifera, Ecklonia cava, Laminaria japonica, Sargassum fulvellum, Sargassum horneri, Sargassum miyabei, Sargassu, thunbergii, Agarum cribrosum and Hizikia fusiformis, Phodophyta; Porphyra yezoensis, Grateloupia elliptica, Lomentaria catenata, Plocamium telfairiae and Gracilaria verrucosa, the highter desmutagenic activity than Rhodophyta and Chlorophyta. E. stolonifera, E. cava and S. miyabei, among Phaeophyta exerted the stronger desmutagenic activity(above 90%/2 ㎎). The ethyl actate, diethyl ether and chloroform extracts except water extracts from E, stolonifera exhibited a high desmutagenic activity. The ethyl acetate extract of E. stolonifera which showed highest acuity was fractionated with SephadexLH20 column chromatography to give active fraction A-7, which showed desmutagenicity of 90%/㎎ against PhIP and 80%/㎎ against MeIQx. The active fraction had the absorbance at 207.7 and 232㎚.
해조류 중의 anti - tumor initiator 및 promoter 의 해석 - 곰피 추출물중의 발암 promotion 억제 인자
박영범(Yeung Beom Park),김인수(In Soo Kim),유승재(Sung Jae Yoo),안종관(Jong Khan Ahn),이태기(Tae Gee Lee),박덕천(Douck Chon Park),김선봉(Seon Bong Kim) 한국수산과학회 1998 한국수산과학회지 Vol.31 No.4
To elucidate anti-tumor promoter from seaweed. the anti-tumor promoting activity of Ecklonia stolonifera, Undaria pinnatifida and Laminaria japonica extracts were determined by Epstein-Barr virus (EBV)-early antigen (EA) induction caused by a tumor promoter, teleocidin B-4. The methanol extracts of seaweed were subsequently fractionated with diethyl ether, distilled water, chloroform and ethyl acetate. Among the solvent fractions tested, chloroform and ethyl acetate fraction of E. stolonifera showed a high anti-tumor promoting activity at the levels of 88.0 and 85.9% by the addition of 20 ㎍/㎖respectively. To characterize anti-tumor promoters from solvent fractions of E. stolonifera, the effects of phenols, chlorophyll derivatives and carotenoids on the anti-tumor promoting activity were investigated. Phenols, such as bromophenol and phloroglucinol showed anti-tumor promoting activity of 57∼66% at 20 ㎍/㎖. Pigments, such as chlorophylls and carotenoids exerted high anti·tumor promoting activities. Chlorophyll a and pheophorbide a exhibited the activity of 77.4% and 66.6% at 5μM/㎖respectively. The active compounds of carotenoids were tentatively identified as lutein and α-cryptoxanthin from the profiles of visible spectra and R_f value of their authentic compounds, and showed anti-tumor promoting activities of 76.9% and 84.4% at dose of 20 ㎍/㎖, respectively.
멸치육 효소 가수분해물의 Angiotensin 전환효소 저해작용
이태기(Tae Gee Lee),박영범(Yeung Beom Park),박덕천(Douck Chon Park),염동민(Dong Min Yeum),김인수(In Soo Kim),구연숙(Yeun Suk Gu),박영호(Yeung Ho Park),김선봉(Seon Bong Kim) 한국수산과학회 1998 한국수산과학회지 Vol.31 No.6
To develop functional food material with angiotensin converting enzyme (ACE) inhibitory peptides, muscle protein of anchovy, Engraulis japonica was hydrolyzed during 48 hrs by digestive proteases such as pepsin, trypsin, α-chymotrypsin, and commercial proteases such as papain, bromelain, complex enzyme, Flavourzyme, Novozym, Neutrase, Protamex and Alcalase. The only 50% ethanol soluble hydrolysates were tested for inhibitory activity against ACE and yield of 50% ethanol soluble peptide-nitrogen (ESPN_(50)). ACE inhibition effects and yield of ESPN_(50) occurred as hydrolysis time increased to 8 hrs. Among those proteases tested, hydrolysates by Alcalase and α-chymotrypsin had greater ACE inhibitory activity (80 and 74%, respectively) with elevated levels of ESPN_(50) (48 and 58 ㎎/㎖, respectively), while Protamex hydrolysates had greater ACE inhibitory activities (73%) with reduced levels of ESPN_(50) (7.2 ㎎/㎖) than others. Amino acid compositions of 50% ethanol solubles obtained from those hydrolysates were rich in glutamic acid, aspartic acid, cysteine and leucine.