Dehydro - L - ascorbic Acid 가 흰쥐 간 조직호흡과 Succinic Dehydrogenase 활성에 미치는 영향

문경애,김현옥,송정석 ( Kyung Ae Moon,Hyun Ock Kim,Chung Suk Song ) 생화학분자생물학회 1975 BMB Reports Vol.8 No.2

Dehydro-L-ascorbic acid (DHA) was added to the liver homogenates of rats and the O₂-consumption was observed. DHA increased the O₂-consumption at low levels of DH A but decreased it at high levels. DHA inhibited the O₂-consumption in the succinic dehydrogenase-cytochrome oxidase system of the liver homogenates of rats, but addition of cytochromeic reversed the inhibitory effect of DHA and tended to increase the O₂-consumption when cytochrome c was added in sufficient quantity. It may be suggested then that DHA stimulates succinic dehydrogenase but inhibits the activity of cytochrome c.

백서 가토 및 닭에 있어서 그 년령에 따라 조직내 Dehydroascorbic Acid Reductase 활성에 대한 연구

김현옥,사혜순,송정석,Kim, Hyun-Ock,Sa, Hae-Soon,Song, Chung-Suk 생화학분자생물학회 1973 한국생화학회지 Vol.6 No.3

백서 가토 및 닭의 여러장기에서 dehydro-L-ascorbic acid reductase(DHA-reductase)의 활성을 측정하고 다음과 같은 결과를 얻었다. 1) 백서 가토 및 닭의 각 동물의 간, 뇌, 신, 소장, 근육, 부신에서 DHA-reductase 활성은 모두 부신에서 높았고, 근육에서 가장 낮았다. 간, 뇌, 소장에서 DHA-reductase 활성은 부신에서 보다는 낮고, 근육에서 보다는 높았다. 그리고 신장에서는 간, 뇌, 소장에서 보다 약간 낮았다. 2) 간에서 DHA-reductase 활성은 백서와 가토에서는 생후 일차 떨어지다가 다시 증가 하였고, 닭에서 생후 처음부터 증가 하였다. 3) 백서에서 DHA-reductase 활성은 cytosol에만 있었다. Dehydro-L-ascorbic acid reductase (DHA-reductase) activities in various tissues of rats, rabbits and chicken were determined and the following results were obtained: 1) Among the DHA-reductase activities in liver, brain, kidney, small intestine, muscle and adrenal of rats, rabbits and chicken, the highest activity was found in adrenal and the lowest activity in muscle of each animal. The DHA-reductase activities in liver, brain and small intestine were lower than that in adrenal, but were higher than that in muscle of each animal and the activity in kidney was slighly lower than those in liver, brain and small intestine. 2) DHA-reductase activities were decreasing immediately after the birth but soon were increasing in liver of rats and rabbits, but the DHA-reductase activity in liver of chicken was increasing immediately after the birth. 3) DHA-reductase activity in rats was found only in cytosol.

흰 쥐에서 비타민 A 흡수에 미치는 비타민 C의 영향

김현옥,김제현,송정석,Kim, Hyun-Ock,Kim, Je-Hyun,Song, Chung-Suk 생화학분자생물학회 1974 한국생화학회지 Vol.7 No.2

백서에 있어서 비타민 C가 비타민 A의 장내 흡수에 미치는 영향에 관하여 관찰하였다. 백서는 백미식, 10% casein 첨가식, 10% casein 식, 30% casein 식 등 단백함량이 다른 여러 식이로 사육하였다. 비타민 A는 실험에 따라 2000 혹은 40,000 I.U.를 매일 tube-feeding 방법으로, 비타민 C는 10mg 씩 매일 복강내 주사로 3~7일간 투여하였다. 바다민 A의 혈액과 간내의 농도는 삼염화안티몬에 의한 색반응을 이용하여 측정하였으며 다음과 같은 결과를 얻었다. 1) 모든 생체내 실험에서 혈청 비타민 A의 농도는 비타민 C에 의하여 증가되었고 특히 30% casein 식이군은 10% casein 식이군에 비하여 비타민 C의 영향이 컸다. 2) 10%, 30% casein 식이군에서 간내 바타민 A 농도는 비타민 C에 의하여 증가되었다. 3) 유리관류 간 실험에서 비타민 C는 혈청과 간의 비타민 A 농도 변화에 아무 영향도 주지 못하였다. 이러한 결과로 보아 바타민 C는 비타민 A의 장내흡수에는 관여하지만 혈청에서 간으로의 이동에는 관여하지 않는 것 같다. The effect of vitamin C on the intestinal absorption of vitamin A was observed in the rat. The various protein content diets (rice. 10% casein added in rice. 10%, and 30% casein) were fed. Vitamin A(2,000-40,0000 IU) and vitamin C(10 mg) at each day were administered for 3-7 days by the tube-feeding and the intraperitoneal injection, respectively. Vitamin A content in serum and liver was determined by the method of Oser et al. (1943). The following results were obtained: 1) Vitamin A content in the serum was increased by vitamin C in the all in vivo experiments, and 30% casein diet enhanced vitamin C effect on the increasing content as compared with 10% casein diet. 2) Vitamin A content in the liver was increased by the vitamin C in 10% and 30% casein diets. 3) Vitamin C did not significantly change the net vitamin A content in serum and liver in the isolated perfused system. These results may indicate that vitamin C may give effect on the enhancement of the intestinal absorption of vitamin A. but not on the circulatory transport into the liver.

Dehydro - L - ascorbic Acid 가 백서 간에서 Vitamin A 대사에 미치는 영향

조무연,김현옥,송정석 ( Moo Youn Cho,Hyun Ock Kim,Chung Suk Song ) 생화학분자생물학회 1976 BMB Reports Vol.9 No.2

The effect of dehydro-L-ascorbic acid (DHA) on the transfer of vitamin A to the liver was observed in the isolated rat liver perfusion. DHA had no effect on the transfer of vitamin A to the liver. The addition of DHA to the perfusate did not raise the level of either oxidized or reduced form of vitamin A in the liver. From this fact, DHA seems to be destroyed as soon as it is absorbed into the liver or not to be absorbed to the liver and rather to be destroyed in the blood.

Dehydro - L - ascorbic Acid 가 L - Ascorbic Acid Oxidase 에 미치는 영향

문경애,김현옥,송정석 ( Kyung Ae Moon,Hyun Ock Kim,Chung Suk Song ) 생화학분자생물학회 1976 BMB Reports Vol.9 No.4

The effect of dehydro-L-ascorbic acid (DHA) on L-ascorbic acid oxidase in the rat liver homogenate was observed with Warburg apparatus and the following conclusions were obtained. L-Ascorbic acid (ASA) and DHA were destroyed considerably even in a state of phosphate buffer solution but when liver homogenate was added to solution both ASA and DHA were protected from destruction probably by the protective effect of the protein of the homogenate. DHA had no effect on the destruction of ASA at low concentration of DHA, but it inhibited the destruction of ASA at high concentration. Inhibition of the destruction of ASA by DHA was perhaps due to the effect of DHA on the cytochrome c.

진동긴박이 비타민 결핍 백서의 전위부 궤양발생에 미치는 영향

김현옥,전유상,김이식,송정석 ( Hyun Ock Kim,Yoo Sang Chun,Ei Sik Kim,Chung Suk Song ) 생화학분자생물학회 1969 BMB Reports Vol.2 No.2

Since the production of hyperplastic ulcers of forestomachs of rats fed on low protein diet by Fibiger in 1913, many investigators produced the same lesions on the rats. In this laboratory, the hyperplastic ulcers of forestomachs of rats fed on low protein diet was produced in a shorter period by shaking the rats. In this experiment, production of ulcers of forestomachs of the riboflavin or thiamine deficient rats by giving shaking stress was investigated. The results were follows; No ulceration of forstomachs of the riboflavin or thiamine deficient rats fed on 20% casein diet was produced by shaking the rats. However, severe hyperplastic ulcers of forestomachs of the thiamine deficient rats fed on 91% rice diet were produced by shaking the rats, while no change of those of rats fed 91% rice diet were observed by the same treatment. So it is concluded that the thiamine deficiency accelerates the production of hyperplastic ulcers of the forestomachs of rats fed on low protein diet.

진동긴박(振動緊迫)이 비타민결핍(缺乏) 자서(自鼠)의 전위부(前胃部) 궤양발생(潰瘍發生)에 미치는 영향(影響)

김현옥,전유상,김이식,송정석,Kim, Hyun-Ock,Chun, Yoo-Sang,Kim, Ei-Sik,Song, Chung-Suk 생화학분자생물학회 1969 한국생화학회지 Vol.2 No.2

Fibiger 가 1913 년(年)에 저단백식이(低蛋白食餌)로 기른 백서(白鼠)의 전위부(前胃部)에 비후성(肥厚性) 궤양(潰瘍) 발생(發生)을 관찰(觀察)한 이래(以來) 많은 학자(學者)들에 의하여 같은 궤양(潰瘍)을 발생(發生)시키었다. 본교실(本敎室)에서는 저단백식이(低蛋白食餌)로 기른 백서(白鼠)에게 진동긴박(振動緊迫)을 가(加)하면 단시일(短時日)안에 전위부(前胃部)에 비후성궤양(肥厚性潰瘍)이 발생(發生)되는 것을 관찰(觀察)하였다. 본실험(本實驗)은 riboflavin 혹은 thiamine 결핍(缺乏) 백서(白鼠)에게 진동긴박(振動緊迫)을 가(加)하고 그 전위부(前胃部) 궤양(潰瘍) 발생(發生) 여하(如何)를 관찰(觀察)한 것이다. Since the production of hyperplastic ulcers of forestomachs of rats fed on low protein diet by Fibiger in 1913, many investigators produced the same lesions on the rats. In this laboratory, the hyperplastic ulcers of forestomachs of rats fed on low protein diet was produced in a shorter period by shaking the rats. In this experiment, production of ulcers of forestomachs of the riboflavin or thiamine deficient rats by giving shaking stress was investigated. The results were follows; No ulceration of forstomachs of the riboflavin or thiamine deficient rats fed on 20% casein diet was produced by shaking the rats. However, severe hyperplastic ulcers of forestomachs of the thiamine deficient rats fed on 91% rice diet were produced by shaking the rats, while no change of those of rats fed 91 % rice diet were observed by the same treatment. So it is concluded that the thiamine deficiency accelerates the production of hyperplastic ulcers of the forestomachs of rats fed on low protein diet.

국민학교 아동의 영양 조사. - 서울과 밀양 국민 학교 아동

조한영,황석귀,김현옥,송정석,Cho, Han-Yung,Whang, Suck-Kuy,Kim, Hyun-Ock,Song, Chung-Suk 생화학분자생물학회 1972 한국생화학회지 Vol.5 No.2

서울 미동국민학교와 밀양 밀주 및 태룡국민학교 5, 6학년 아동들에게 1967년 가을과 1968년 봄에 혈액내 carotene, 비타민 A 및 비타인 C 와 뇨중 thiamine, riboflavin 과 N-methylnicotinamide 배설량을 측정하여 다음과 같은 결론을 얻었다. 1) 밀주와 태룡국민학교 아동들의 혈액내 carotene 농도는 가을에 미동국민학교 아동들보다 윌등히 높았다. 이는 아마 가을에 지방아동들이 감을 많이 먹기 때문인것 같다. 봄에는 지방과 서울이 다같이 비슷함 농도를 보여 주었다. 2) 비타민 C 혈액내 농도는 가을에 있어서 서울과 지방아동들에게서 다 높았고 특히 지방 아동들에게서 높았으나 봄에 와서는 서울 아동들은 월등히 떨어졌었다. 이는 서울 아동들이 겨울에 채소를 적게 먹기 때문인것 같다. 3) Thiamine, ribof1avin, N-methylnicotinamide 뇨중 배설은 지방 아동등에서 약간 높았고 가을보다 봄에 뇨중 배설량은 서울과 지방 아동들이 다 같이 높았다. In autumn of 1967 and spring of 1968, determinations of carotene, vitamin A and vitamin C in blood and the excretion of thiamine, riboflavin and N-methylnicotinamide in urine were carried out on the 5th and 6th grade pupils of primary schools of Midong in Seoul and Milchoo and Taeryong in Milyang, Gyunsang Namdo Province, and the following conclusion was obtained. 1. The carotene level in blood of children of Milchoo and Taeryong was excellently higher than that in blood of children of Midong in autumn. This is probably due to higher intake of persimmon of rural children than that of Seoul children and the carotene blood level in spring was almost same between the children of Seoul and the rural area. 2. The vitamin C level was high in blood of both children of Seoul and Milyang, especially high in that of rural children but in spring the vitamin level was severely reduced in blood of Seoul children. This is probably due to less intake of vegetables during winter. 3. The amount of urinary excretion of thiamine, riboflavin and N-methylnicotinamide was slightly larger in the rural children than in those of Seoul, and it was larger in spring than in autumn.

국민학교 아동의 영양 조사 서울과 밀양 국민 학교 아동

조한영,황석귀,김현옥,송정석 ( Han Yung Cho,Suck Kuy Whang,Hyun Ock Kim,Chung Suk Song ) 생화학분자생물학회 1972 BMB Reports Vol.5 No.2

In autumn of 1967 and spring of 1968, determinations of carotene, vitamin A and vitamin C in blood and the excretion of thiamine, riboflavin and N-methylnicotinamide in urine were carried out on the 5th and 6th grade pupils of primary schools of Midong in Seoul and Milchoo and Taeryong in Milyang, Gyunsang Namdo Province, and the following conclusion was obtained. 1. The carotene level in blood of children of Milchoo and Taeryong was excellently higher than that in blood of children of Midong in autumn. This is probably due to higher intake of persimmon of rural children than that of Seoul children and the carotene blood level in spring was almost same between the children of Seoul and the rural area. 2. The vitamin C level was high in blood of both children of Seoul and Milyang, especially high in that of rural children but in spring the vitamin level was severely reduced in blood of Seoul children. This is probably due to less intake of vegetables during winter. 3. The amount of urinary excretion of thiamine, riboflavin and N-methylnicotinamide was slightly larger in the rural children than in those of Seoul, and it was larger in spring than in autumn.

백서에 있어서 비타민A 흡수 및 이동에 미치는 비타민C 의 영향 (제2보)

김현옥,송정석,사혜순 생화학분자생물학회 1978 BMB Reports Vol.6 No.3