두경부 편평상피세포암 세포주에서 세포주기조절인자의 활성 및 이상 : 후두편평상피세포암에서 종양억제유전자 CDKN2 유전자의 발현이상

송시연 ( Si Youn Song ),한태희 ( Tae Hee Han ),배창훈 ( Chang Hoon Bai ),김용대 ( Yong Dae Kim ),송계원 ( Kei Won Song ) 영남대학교 기초/임상의학연구소 2005 Yeungnam University Journal of Medicine Vol.22 No.2

Background: Cyclin-dependent kinase (CDK) inhibitors are family of molecules that regulate the cell cycle. The CDKN2, a CDK4 inhibitor, also called p16, has been implicated in human tumorigenesis. The CDKN2 inhibits the cyclin/CDK complexes which regulate the transition from G1 to S phase of cell cycle. There is a previous report that homozygous deletion of CDKN2 region on chromosome 9p21 was detected frequently in astrocytoma, glioma and osteosarcoma, less frequently in lung cancer, leukemia and ovarian cancer, but not detected in colon cancer and neuroblastoma. However, little is known about the relationship between CDKN2 and laryngeal cancer. Therefore this study was initiated to investigate the role of CDKN2 in human laryngeal squamous cell carcinoma development.1) Materials and methods: We used 5 human laryngeal carcinoma cell lines whether they have deletions or losses of CDKN2 gene expression by DNA-PCR or RT-PCR, respectively. We examined 8 fresh frozen human laryngeal cancer tissues to detect the loss of heterozygosity (LOH) of CDKN2. PCR was performed by using microsatellite markers of short arm of human chromosome 9 (D9S126, D9S144, D9S156, D9S161, D9S162, D9S166, D9S171, D9S200 and D9SIFNA). For informative cases, allelic loss was scored if the signal of one allele was significantly decreased in tumor DNA when compared to the same allele in normal DNA. Results: The CDKN2 DNA deletion was observed in 3 cell lines. The CDKN2 mRNA expression was observed in only one cell line, which was very weak. LOH was detected in 7 cases (87.5%). Conclusion: These results suggest that CDKN2 plays a role in the carcinogenesis of human laryngeal squamous cell carcinoma.

이구개지단 증후군 1례

최경종,은선진,이성형,송계원 영남대학교 의과대학 1991 Yeungnam University Journal of Medicine Vol.8 No.1

In 1962, a case of "generalized skeletal dysplasia with multiple anomalies" was reported by Taybi as a new clinical syndrome. The most characteristic features of the syndrome seemed to be hearing loss, cleft palate and peculiar digital anomalies, so, the syndrome designated as otopalatodigital syndrome by Dudding, et al. Recently, The authors have experienced a case OPD syndrome who visited Yeungnam University Hospital with the chief complaint of hypernasality, and underwent pharyngoplasty for correction of VPI. We present here a new case and review the literature on the subject.

Testosteron Cyclopentylproponate의 造血促進作俑에 關한 硏究

宋啓源 慶北大學校 醫科大學 1971 慶北醫大誌 Vol.12 No.2

The author studied the erythropoietic response to testosterone cyclopentylpropionate as indicated by erythropoietin activity as well as marrow nucleated red cell count in albino rats and the following results were obtained. (1) The albino female rats injected with a single dose of 60 ㎎/㎏ of body weight of testosterone cyclopentylpropionate intramuscularly showed an enhanced erythropoietin activity and marrow nucleated red cell count on the third day reaching their peaks by the seventh day and followed by a gradual decline. (2) The optimum single dose of testosterone cyclopentylpropionate for erythropoietic response was 120 ㎎/㎏ of body weight among the groups of 30 ㎎/㎏, 60㎎/㎏, 120㎎/㎏ and 240㎎/㎏ of body weight. (3) The erythropoietin activity and marrow nucleated red cell count measured one week after a single dose of 60 ㎎/㎏ of body weight of testosterone cyclopentylpropionate in male and female rats revealed the latter sex to have greater response than the former.

분리 세포의 단층세포 배양법에 의한 인체 비점막 상피세포의 배양

박호선,송시연,김용대,민명기,서장수,송계원 영남대학교의과대학 1998 Yeungnam University Journal of Medicine Vol.15 No.2

비중격 만곡증 환자의 비내수술시 체취한 정상하비갑개 점막조직으로부터 상피세포만을 분리세포의 단층배양법(monolayer culture of dissociated cells)으로 배양하여 비점막 상피세포 배양법을 정립하고 또한 배양된 세포가 상피세포임을 동정하기 위하여 간접 면역형광항체법으로 상피세포 특유의 cytokeratin을 확인하였고 투과전자현미경으로 상피세포만이 가지고 있는 교소체(desmosome) 및 장세사(tonofilament) 등을 확인하였으며 6회까지 계대배양을 할 수 있었다. 향후 본 연구에서 배양된 비점막 상피세포를 이용하여 알레르기성비염 등과 같은 비점막 염증성 질환의 병태생리에서 상피세포의 역할에 대한 연구와 호산구등 염증세포의 침윤과 상피세포의 손상에 접착분자 및 cytokine의 상호작용에 관한 연구가 계속될 수 있을 것으로 기대된다. Different techniques for culturing respiratory epithelial cells have been developed to overcome the limitations of studies on in vivo and on bioptic material. Traditionally, culture systems are divided into organ cultures, explant cultures and dissociated cell cultures. The first two contain both epithelial and non-epithelial cells. However, in monolayer cultures of dissociated cells only epithelial cells are present, the effects observed are caused by a pure epithelial responses. The purpose of this study is to establish primary culture method of human nasal epithelium (HNEC) by monolayer culture of dissociated cells to evaluate the role of the epithelial cells in the allergic and non-allergic nasal inflammatory reactions. HNEC was prepared by primary culture method of monolayer culture of dissociated cells from human inferior nasal turbinate mucosa of septal deviation patients. Primary cultured cells were characterized by indirect immunofluorescence assay and transmission electron microscopy. The immunoreactivities of cytokeratin-pan and cytokeratin No. 8 were observed in cultured HNEC. However, the immoreactivities of vimentin and von Willebrand factor were not observed in cultured HNEC. The tonofilaments and desmosome were observed in cultured HNCE. The cultured epithelial cells were identified to be pure nasal epithelial cells. The monolayer culture of dissociated cells could successfully be employed for further study to investigate the role of the epithelial cells in allergic or non-allergic nasal inflammatory diseases.

두경부 편팡상피세포암 세포주에서 세포주기 조절인자의 활성 및 이상:후두편평상피세포암에서 종양억제 유전자 CDKN2 유전자 발현이상

송시연,한태희,배창훈,김용대,송계원 영남대학교 의과대학 2005 Yeungnam University Journal of Medicine Vol.22 No.2

Background: Cyclin-dependent kinase (CDK) inhibitors are family of molecules that regulate the cell cycle. The CDKN2, a CDK4 inhibitor, also called p16, has been implicated in human tumorigenesis. The CDKN2, inhibits the cyclin/CDK complexes which regulate the transition from G1 to S phase of cell cycle. There is a previous repot that homozygous deletion of CDKN2 region on chromosome 9p21 was detected frequently in astrocytoma, glioma and osteosarcoma, less frequently in lung cancer, leukemia and ovarian cancer, but not detected in colon cancer and neuroblastoma. However, little is known about the relationship between CDKN2 and laryngeal cancer. Therefore this study was initiated to investigate the role of CDKN2 in human laryngeal squamous cell carcinoma development. Materials and methods: We used 5 human laryngeal carcinoma cell lines whether they have deletion or losses of CDKN2 gene expression by DNA-PCR or RT-PCR, respectively. We examined 8 fresh frozen human laryngeal cancer tissues to detect the loss of heterozygosity(LOH) of CDKN2 PCR was performed by using micosatellite markers of short arm of human chromosome 9 (D9S126, D9S144, D9S156, D9S161, D9S162,D9S166, D9S166, D9S171,D9S200 and D9SIFNA).For informative cases, allelic loss was scored of the signal of one allele was significantly decreased in tumor DNA when compared to eh same allele in normal DNA. Results: the CDKN2 DNA deletion was observed in 3 cell lines. The CDKN2 mRNA expression was observed in only one cell line, which was very weak. LOH was detected in 7 cases(87.5%) Conclusion: These results suggest that CDKN2 plays a role in the carcinogenesis of human laryngeal squamous cell carcinoma

最近急增한 耳鼻咽喉科領域의 梅毒에 대하여

宋啓源,李在昌,金致益,鄭泰洙,李養璿 대한이비인후과학회 1967 대한이비인후과학회지 두경부외과학 Vol.10 No.1

I^(134) 投與가 흰쥐의 造血能에 미치는 影響에 對하여

宋啓源 대한이비인후과학회 1967 대한이비인후과학회지 두경부외과학 Vol.10 No.4

알레르기성 비염에 대한 Ketotifen(Zaditen)의 임상적 효과

송계원,박문흠,서장수,김용대,은선진 영남대학교 의과대학 1990 Yeungnam University Journal of Medicine Vol.7 No.1

저자들은 1988년 5월 1일부터 12월 31일까지 영남대학교 의과대학 부속병원 이비인후과에 내원한 알레르기성 비염환자 39명을 대상으로 Ketotifen 1일 투 2㎎씩 8주동안 투여한 뒤 재채기 발작, 수성비루, 비폐색, 비강내 이학적 소견, 효과 발현시기, 부작용 등을 관찰하여 다음과 같은 결과를 얻었다. 1) 전반적 개선도는 개선 이상이 66.7%, 약간 개선 이상이 87.2%로 유의한 증상의 호전이 있었다. 2) 증상별로 재채기 발작은 73.5%, 수성비루 71%, 비폐색 58%에서 증상의 호전이 있었으며 재채기 발작과 수성비루에 대해 더욱 효과적이었다. 3) 증상의 발현시기는 1주이내 35.7%, 2주이내 53.8%, 3주이내 66.7%, 4주이내 74.4%, 8주이내 87.2%로 나타냈다. 4) 비점막 소견의 유의한 호전은 없었다. 5) 부작용은 1례에서 경한 복통과 설사가 있어서 투약을 중지하였다. 이상과 같이 Ketotifen은 알레르기성 비염의 치료에 효과적이며 비교적 안전하게 사용할 수 있는 약제라고 생각할 수 있다. Ketotifen, a benzocycloheptathiophene, has an orally effective antiallergic as well as antihistaminic properties. In pervious studies, Ketotifen has shown encouraging results in patient with allergic rhinitis, either perennial or seasonal. 39 patients with allergic rhinitis had been treated with Ketotifen 1 mg twice daily for 8 weeks. And we obtained following results. 1) The efficacy rate in sneezing attack was 73.5%, in nasal discharge 71%, in nasal obstruction 58%. 2) Some improvements in at least one of three major symptoms were noted within 1 week in 30.7%, within 2 weeks in 55.8%, within 3 weeks in 66.7%, within 8 weeks in 87.2%. 3) Physical findings such as colour, swelling of turbinate, character of rhinorrhea were not improved singnificantly. 4) Side effect was observed only in one patient with abdominal pain and diarrhea, which was subsided after interruption of administration. These results suggested that Ketotifen was effective in tratment of allergic rhinitis.

비인강 혈관섬유종 1례

송계원,윤석근,박영식 영남대학교 의과대학 1984 Yeungnam University Journal of Medicine Vol.1 No.1

16세 남자에서 발생한 비인강 혈관섬유중 1례를 수술적 치료하여 경험하였기에 문헌고찰과 아울러 증례 보하는 바이다. Nasopharyngeal angiofibroma is a relatively rare, but is a well known tumor that occurs almost in male, most frequently during the adolescent stage. Its character is histologically benign but clinically malignant because of locally invasive character to the adjascent structures, massive bleeding tendency during surgical procedure and high recurrence rate after treatment. Recently many advancements in diagnostic method and surgical technique has reached satisfactory result in treating this difficult tumor. The authors present a case of nasopharyngeal angiofibroma in 16 years old male patient, who was received surgical removal of the tumor through the transpalatal approach after full diagnostic evaluation and preoperative hormonal therapy.

前頭洞骨腫의 1症例

池重敏,宋啓源,金鎭吉,趙秀子 대한이비인후과학회 1967 대한이비인후과학회지 두경부외과학 Vol.10 No.4