고분염 분석법 ( high - resolution banding ) 에 의한 한우 염색체의 표준 표지 설정

손시환,이재익 ( S . H . Sohn,J . I . Lee ) 한국축산학회 1998 한국축산학회지 Vol.40 No.5

Using the high-resolution banding technique, a karyotyping for Korean Cattle was performed. Blood samples were collected from 119 male cattle that had been stationed at the National Livestock Research Institute for progeny test and then were prepared to in vitro cultures followed by karyotyping; morphology and G-, Cbanding patterns of prophase, prometaphase and metaphase chromosomes were analyzed. All their 58 autosomes revealed almost a zero value of centromeric index, indicating that they are acrocentric; sex chromosomes X and Y were submetacentric and metacentric chromosome, respectively. Chromatin condensation, which increased with the progression from prophase to metaphase in all chromosomes, was greater in longer chromosomes than in shorter ones. Prophase and metaphase chromosomes exhibited 544 and 303 G-bands per haploid, respectively, with variable band intensity. This number of G-bands in prophase was much greater than G-band that can be obtained by a conventional method, implicating an improved karyotyping for Korean Cattle. Moreover, autosomes exhibited the C-bands at or near centromere, whereas sex chromosomes had variable numbers of them on variable locations. The proportion of constitutive heterochromatin, ranging 13∼27%, increased with decreasing chromosomal length and also with increasing chromatin condensation. We propose these results as new standard karyotyping for Korean Cattle

유우의 산유능력에 관여하는 형질들의 유전력 , 유전상관 및 상관반응 추정에 관한 연구

손시환,오봉국,이정구,조윤연,오대균 ( S . W . Sohn,B . K . Ohh,J . K . Lee,Y . Y . Cho,D . K . Ohh ) 한국축산학회 1983 한국축산학회지 Vol.25 No.1

The objects of this study were to estimate the heritabilities, phenotypic and genetic correlations among productive traits and to find optimum selection method based on genetic parameters for each trait in dairy cattle. The data used in this study were obtained from the Holstein Friesian cows of The National Livestock Breeding Station. There were 1429 records from 354 cows, representing 44 sires. The data collected from 1969 through 1981. The traits studied were Milk Yield, Fat Yield, Fat Percentage, Peak Yield and Peak Lactation Period. The results obtained in this study were summarized as follows; 1. In the average performance of productive traits, milk yield was 4820.32 ± 1152.05 ㎏, fat yield 170.29 ± 41.62 ㎏, fat percentage 3.54 ± 0.33 %, peak yield 24.31 ± 5.75 ㎏ and peak lactation period 44.12 ± 27.39 day. As compared with average foreign report, above average performances showed low trend except peak lactation period. 2. The heritability estimates from sire component were 0.211 for milk yield, 0.249 for fat yield, 0.520 for fat percentage, 0.236 for peak yield, 0.199 for peak lactation period. As a result, these traits are not high heritable characters except for fat percentage. 3. The genetic correlation coefficient of milk yield with fat yield was 0.841, milk Meld with fat percentage -0.098 and fat yield with fat percentage 0.458. Relationship of milk yield was measured to be 0.518 with peak yield, 0.744 with peak lactation period. Expected genetic change in productive traits based on a phenotypic selection differential was also studied. Therefore, direct selection may be more effective than indirect selection.

혈액세포를 이용한 염색체 분리 분석에 관한 방법적 고찰

손시환,정구민,Sohn, S.H.,Chung, K.M. 대한생식의학회 1994 Clinical and Experimental Reproductive Medicine Vol.21 No.2

This study was carried out to develop the methodology of chromosome preparation from blood cultures in mammals which included human, mouse, cattle and pig. For karyotyping, 0.5-5.0ml of peripheral blood were collected and cultured. The satisfactory results were obtained from macroculture and microculture in all species. In culture, the patterns of cell growth were no difference among media except serum concentration and mitogen supplement. The presence of mitogen and fetal bovine serum in medium significantly affected the mitotic index. The optimal culture condition was 37$^{\circ}C$ for 3 days. And the concentration of colcemid and reincubation time also affected the chromosome morphology. In harvest, chromosome patterns were mainly affected on hypotonic treatment which included treated time and temperature, dropwise of fixative solution, and drying after slide preparation.

혈액배양에 의한 일본산 메추리의 염색체 분리기법

손시환(S . H . Sohn),(N . S . Fechheimer),(B . K . Ohh) 한국축산학회 1990 한국축산학회지 Vol.32 No.12

N/A

G - banding 에 의한 닭 염색체 표식인자에 관한 연구

손시환(S . H . Sohn),오봉국(B . K . Ohh) 한국축산학회 1987 한국축산학회지 Vol.29 No.12

The G-banding patterns of chicken chromosomes had characteristic and distinctive bands along the individual chromosome arms. Especially, the six larger pairs of chicken chromosomes were easily identifiable. By the karyotype and densitometric analysis, the forms of G-banding marker in each breed were as followed. Most breeds had a similar chromosome configuration although each macrochromosome had different pattern in band number, size and darkness. This could be explained as a consequence of chromosome condensation during mitosis and of genetic material differences.

닭 ( Gallus domesticus ) 염색체의 수 및 형태 고찰

손시환(S . H . Sohn),오봉국(B . K . Ohh) 한국축산학회 1987 한국축산학회지 Vol.29 No.12

The chromosome morphological structure, size and number were studied by early embryos of chicks. The chromosomes of 100 early embryos which were derived from a pure line of Single Comb White Leghorn, White Cornish, and crossbreds of these breeds were examined. In the results, the total number of chromosomes appeared to be 72-78. But the modal number for the diploid chromosome was at least 78. These chromosomes were consist of 6 pairs of macrochromosomes, 32 pairs of microchromosomes and ZW or ZZ sex chromosomes. In addition the chromosome size and shape were studied by the centromeric index and relative length of pure breeds and crossbreds. The differences of morphological figures were not significant in centromeric index and relative length. Instead, this pattern was very similar among breeds except that the one individual of the cross between Leghorn (♂) and Cornish (♀) produced triploid embryos. The gonosomic complement was ZWW. The results of the present investigation of chromosome pairs were as follows. 1. Pair 1 and 2; metacentric and submetacentric chromosomes which could be clearly distinguished from each other by size. 2. Pair 3 and 4; acrocentric chromosomes of similar length but the 4th pair had a distinct short arm which was not present in the 3rd. 3. Pair 5; metacentric sex chromosomes. Z chromosome had relative 5th length but the W chromosome had slightly shorter length than 7th pair of chromosomes. 4. Pair 6; acrocentric chromosomes similar in shape to pair 3 but of little more than half the size. 5. Pair 7 and 8; acrocentric chromosomes, but the 7th pairs had a definite short arm. 6. Pair 9; similar length to the 7, 8 pairs but had a medially placed centromere. 7. Microchromosomes of 30 pairs; nearly all acrocentric chromosomes which appeared as paired dots.

가시오갈피와 두충의 첨가 급여가 브로일러의 생산 능력, 혈장 생화학 지표 및 텔로미어 함량에 미치는 영향

손시환(S. H. Sohn),장인석(I. S. Jang),문양수(Y. S. Moon),김영주(Y. J. Kim),이수희(S. H. Lee),고영현(Y. H. Ko),강선영(S. Y. Kang),강혜경(H. K. Kang) 한국가금학회 2008 韓國家禽學會誌 Vol.35 No.3

한국 꿩 ( Korean Ring - necked Pheasant ) 의 형태적 특징과 핵형 분석

최성복(S . B . Choi),손시환(S . H . Sohn),정선부(S . B . Chung),정일정(I . C . Cheong),오희정(H . J . Ohh) 한국축산학회 1991 한국축산학회지 Vol.33 No.6

A preliminary study of phenotypic characteristics and karyotype of Korean ring-necked pheasant was carried out. The phenotypic characters of interest were mainly feather color and body conformation. In phenotypic characteristics. the feather color patterns and body type showed significant differences in male and female. A distinctive feature of Korean ring-necked pheasant male had red skin around eyes. white ring neck. and brownish-yellow color in whole body feather In contrast. a female bad mixed color of dark brown and buff in whole body feather. Through the karyological analysis from carp embryonic tissues the diploid complements of Korean ring-necked pheasant had a total of 82 chromosomes which were mostly acrocentric chromosomes except metacentric chromosome I and Z sex chromosome. The morphologic structures of these chromosomes were almost telomeric acrocentric. However, chromosome 5 and W sex chromosome were the acrocentric chromosomes with a short arm. The centromeric indices of chromosome I and Z sex chromosome were 39% and 42% respectively. The relative lengths of these chromosomes were 32.5% and 13.3% respectively.

Telomere Length and Telomerase Activity of Korean Native Chicken

D. S. Choi,M. J. Lee,J. A. Park,Y. J. Kim,C. Park,I. S. Jeon,S. H. Sohn(손시환) 한국가금학회 2006 한국가금학회 정기총회 및 학술발표회 Vol.23 No.-

The telomere length and telomerase activity of Korean Native Chicken cells were analyzed along with aging. The cells were collected from brain, heart, liver, kidney and testis tissues during physiological stages. Telomere length was analyzed by Q-FISH using the telomeric DNA probe. Telomerase activity was performed by TRAP assay. The amount of telomeres on chicken cells was decreased along with aging in most tissues. Furthermore, telomere quantity was significantly different among tissues. The telomeres in gonadal cells had much more than those of other cells. The telomerase activity was down-regulated in cells of brain, heart and liver tissues. Whereas gonadal cells showed a constitutive telomerase activity during all stage of life. In conclusions, the telomere quantity and telomerase activity in chicken are closely relate to cell proliferation and tissue specificity during developmental stages and aging.

리포좀을 이용한 형질전환 닭 생산에 대한 연구

변승준,박철,양보석,김태윤,손시환,김상훈,전익수,Byun S. J.,Park C.,Yang B. S.,Kim T. Y.,Sohn S. H.,Kim S. H.,Jeon I. S. 한국가금학회 2004 韓國家禽學會誌 Vol.31 No.4

본 연구는 기존의 형질전환 닭 생산방법 중의 하나인 1세 포기 수정란에 유전자를 직접 주입하는 유전자 미세주입방법을 개선할 목적으로 리포좀과 외래 표지 발현유전자인 GFP를 사용하여 외래유전자의 핵전이 효율성과 주입된 외래 유전자의 발현의 지속성을 닭의 배자에서 검증하고자 시도하였다 외래유전자는 배반엽 단계 혹은 1세포기 수정란의 세포질에 리포좀과 유전자의 혼합물 혹은 오직 유전자만을 미세주입을 하였다. 연구 결과들은 리포좀을 사용한 경우 naked DNA에 비하여 배반엽 단계와 1세포기 수정란 모두에서 효율적으로 외래 유전자를 핵내로 도입할 수 있음을 배양 3과 4일차 닭의 배자에서 GFP발현 양상을 통하여 확인하였다. 또한 주입된 외래 유전자에 의해 만들어진 GFP는 배자에서 일주일 정도 지속적으로 발현됨이 관찰되었다. 리포좀 방법은naked유전자 주입 방법에 비해 1세포기와 배반엽 단계 수정란 모두에서 효율적으로 외래 유전자를 핵내로 이동시키는 능력을 가지나, 주입된 유전자의 염색체 삽입에는 큰 영향을 미치지 않는 것으로 판단된다. 따라서 닭의 수정란에서 리포좀 방법은 외래유전자 도입에 유용한 수단으로 이용되어질 수 있을 것으로 사료된다. Microinjection of DNA is a general method for generating transgenic animals, but the rate of transgenesis in chickens is very low. So it was carried out to investigate the efficiency of liposome-mediated gene transfer in stage one cell of chicken embryo with GFP expression vector. In order to determine efficiency and duration of the introduced foreign gene, it was microinjected DNA with liposome or naked DNA into the germinal disc of stage one cell or stage-X chicken embryo. Analysis of reporter gene expression in day-4 embryos showed that GFP expression was observed only in the liposome-mediate embryo groups and detectable up to day-8 embryos. The results suggest that stable integration of the introduced gene using liposome is a rare event. Nevertheless the liposome-mediated gene transfer may be a useful method to transfer a foreign gene into the stage one cell of chicken embryos.