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正常마우스 副腎皮質細胞更新系에 미치는 生後齡의 影響에 關한 自記放射法的 硏究
成耆晙,韓萬熙 최신의학사 1970 最新醫學 Vol.13 No.10
The effects of successive increase of postnatal ages on the adrenal cortex from the normal albino mouse have been investigated by means of the microradioautographic technique after the intraperitoneal administration of single dose of 0.8 microcurie/mg of body weight of tritiated thymidine. Sixty-eight mice(of which 29 were male and the rest of them were 39 of female) were put into six age groups. The age range was from 4-day old suckling to the age more than 50-week old of mature adult. Each of the mice was sacrificed at 120 minutes after the administration of the radioactive i s6lope, and the stripping film method was adopted after the histological preparation of three micra section from the adrenal gland tissue. The microslides were stained with Ehrlich's acid haematoxylin after 28 days exposure in room temperature. 1. Regardless the sex,. high cell renewal frequencies from the suckling young age groups except the 3 week old group were contrasted with those of the adult groups of low. 2. Of the suckling young, successive increase of the postnatal age was induced decrease of the cell renewal frequencies of the fascicular zone. This fact was reversed in their glomerular zone. 3. Of the adult male, decrease of the cell renewal rates of the glomerular zone with increase of the postnatal age were accompanied by concommitant increase of those in their reticular zone. 4. It appears to be that the adrenocortical cell renewal frequencies were modified by the sex hormones through both the ovarian cyclic change and related postnatal ages. 5. The lowest cell renewal rate in the 3-week old group suggests further detection is needed.
成耆晙 최신의학사 1968 最新醫學 Vol.11 No.9
The detection of cell renewal patterns in the brain cortex from 70 normal adult male mice has been investigated by means of radioautography following the single dose of the. intravenous administration' of H3- thymidine C0.8 uc/grm of body weight. 1. The renewal of the cells has been observed in the brain cortex. ` 2. Among the cells in the brain cortex, such as neuron and glia cells of the cerebral cortex,- Purkinje cells and the other cells of the cerebellar cortex, only the glial cell has the diurnal cycle in its proliferative activity. 3. The first division of all the observed cells were completed by 7 hours after the administration of H^(2) thymidine. 4. These results suggest that further studies are necessary.