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고슴도치 위장관의 Gastrin(G)세포, Glucagon(L)세포, Somatostatin(D)세포 및 Cholecystokinin(I)-8세포의 면역세포화학적 연구
최월봉,원무호,박형진,서지은 한국통합생물학회 1987 동물학회지 Vol.30 No.2
Recently, the researches on the enteroendocrine cells of vertebrates have made a remarkable advance by the immunocytochemical methods. This study was attempted to investigate the topographical distributions and the shapes of gastrin, glucagon, somatostatin and cholecystokinin-8 immuno-reactive cells in the gastrointestinal tract of the Korean hedgehog, Erinaceus koreanus. For light-microscopical examination of immunocytochemistry, the tissue specimens taken from the various portions(body and pyloric protion of stomach, duodenum, jejunum, ileum and rectum) were fixed in glutaraldehyde-picric acid-acetic acid (GPA) or 10% neutral buffered formalin solutions. For the demonstration of immunoreactive cells, the paraffin sections (6$\mu$m) were immunocytochemically identified by PAP procedure (Sternberger, 1979) with gastrin, glucagon, somatostatin and cholecystokinin-8 antisera. Gastrin-immunoreactive cells were mainly distributed in the pyloric portion of stomach and were a few in the duodenum and jejunum. The shapes of these cells were round or oval in the pyloric portion and pyramidal in the small intestine. Glucagon-immunoreactive cells were sparsely distributed in the only small intestine. The shapes of these cells were mainly pyramidal. Somatostatin-immunoreactive cells were a few in the pyloric portion and duodenum, and were sparsely distributed in the body of stomach and jejunum. The shapes of these cells were round or oval in the stomach and oval or pyramidal in the small intestine. Cholecystokinin-8-immunoreactive cells were sparsely distributed in the only small intestine. The shapes of these cells were mainly oval or pyramidal.
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최월봉,윤상선,고병문,조승묵,남성안,최창도,Choi, Wol-Bong,Yoon, Sang-Seon,Ko, Byoung-Moon,Jo, Seung-Mook,Nam, Seong-Ahn,Choi, Chang-Do 한국현미경학회 1997 Applied microscopy Vol.27 No.4
The substantia nigra of the Mongolian gerbil was studies by tyrosine hydroxylase immunohistochemistry and immunoelectron microscopy with preembedding method. The purpose was to obtain information on the distribution and ultrastructure of the Tyrosine hydroxylase immunoreactive and dopaminergic neurons in the substantia nigra, in order to provide the necessary background for the gerbil. Large number of tyrosine hydroxylase immunoreactive neurons were located in the compact part of substantia nigra. Findings in the gerbil, compared to observations in the other species, included the presence of prominent bundles of tyrosine hydroxylase immunoreactive cytoplasmic processes passing in the dorsoventral direction from pars compacta into pars reticulata at middle and caudal levels of the substantia nigra, and the presence of a distinct tyrosine hydroxylase immunoreactive substantia nigra pars lateralis. Tyrosine hydroxylase immunoreactive neurons had well-developed cell organelles, especially rough endoplasmic reticulum, free ribosome and poly-ribosome, and showed the infoldings of the nuclear envelope. We anticipate that the present description of the cellular organization of the tyrosine hydroxylase immunoreactive dopaminergic area in the substantia nigra of gerbil will be useful for the animal experimental model of Parkinson's disease.
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배추흰나비 후뇌에 대한 전자현미경적 연구 2. 연접기구
최월봉,서지은 한국곤충학회 1985 Korean journal of entomology Vol.15 No.1
저자들은 각종 동물의 소뇌의 계통분류학적 계열에 따른 전자현미경적 연구의 일환으로서 배추흰나비 (pieris repae Linne) 후뇌를 연구대상으로 하여 neuropil을 형성하는 신경종말의 연접형태의 미세구조를 전자 현미경으로 관찰하였던 바 다음과 같은 결론을 얻었다. 배추흰나비의 후뇌에서는 축삭-세포 체연접은 전연 볼 수 없었으며 축삭-수상돌기연접만이 이루어져 있었다. 축삭-수상돌기 연접은대 및 소구형의 연접소포와 타원형의 연접소포를 가진 3형의 축삭종말로서 연접을 하고 있었다. For the critical point of view. since the phylogenetical approach will give the ultimate value in the correlative study between structural and functional bases, the present study was carried out to make an electron microscopic analyses of the synaptic organizations of the tritocerebrum in the butterfly (Pieris rapae Linne) from Insecta. Samples for electron microscopic study were fixed by immersion with 1% paraformaldehyde 1% glutaraldehyde mixture (phosphate buffer, pH 7. 4), and final tissue blocks trimmed under dissecting microscope were osmicated (1% OsO4). They were embedded in Araldit or Epon 812, and ultra-thin sections were made by LKB-III ultratome following the inspection of semi-thin sections stained with toluidine blue-bolax solutions. Ultra-thin sections contrasted with uranyl acetate and lead citrate were observed with JEM 100B or Hitachi 500 electron microscope We classified the boutons based on the size and shape of synaptic vesicles, the state of synaptic organelles and synaptic junctions. In the tritocerebrum of the butterfly no axo-somatic synapsis was observed and there was only axo-dendritic synapsis. In this case, the synaptic boutons contained three types of vesicles, large and small round, and flat.
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고슴도치 췌장 내분비의 Glucagon(A),Insulin(B),Somatostatin(D)및 Pancreatic Polypeptide(PP)
최월봉,최창도,원무호,서지은,김남중 한국통합생물학회 1988 동물학회지 Vol.31 No.2
고슴도치의(Erinaceus koneanus)췌강 각 부위별에 출현하는 glucagon(A)세포,insulin(B)세포, somatostatin(D)세포 및 pancreatic polypeptide(PP)세포 등 4종의 내분비세포의 출현빈도, 분포상태, 모양 및 크기 등을 관찰하고자 췌장을 비장부와 십이지장부로 구분하고 이를 각각 근위부, 중간부 및 원위부로 세분한 후 면역세포화학적 방법을 시도하여 광학현미경하에서 검경하였던 바 다음과 같은 결론을 얻었다. Glucagon(A)세포의 크기는13 $\mu$ m x 9.5 $\mu$ m였고 그 모양은 원형, 난원형 및 추체형을 띠고 있었으며 췌도의 주변부와 외분비 실질사이에 분포하고 있었다. A세의 출현빈도는 비장부의 모든 부의에서 24.5%-30.5%, 십이지장부의 원위부에서 30.1%로 관찰되었으나 십이지장부의 근위부와 중간부에서는 2.6%-5.2%로 관찰되었다. Insulin(B)세포의 크기는 11.6$\mu$m x 9.4$\mu$m로서 원형 또는 난원형을 띠었고 췌도내에 균등하게 분포하고 있었으며, 비장부의 모든 부위와 십이지장의 원위부에서는 63.5%-68.9%, 십이지장부의 근위부와 중간부에서는 35.9%-55.6%로 출현하였다. Somatostatin(D)세포는 크기가 12.6$\mu$m x 9.1 $\mu$m로서 주변부와 외분비 실질사에 나타났으며 세포의 출현빈도는 비장부와 십이장부의 모든 부위에서 2.6%-5.9%로서 소수 관찰되었다. Pancreatic polypeptide(PP)세포는 크기가 12.8$\mu$ m x 8.5 $\mu$m였고 세포의 모양은 원형, 난원형 및 추체형 등 다양하였으며 췌도의 주변부와 외분비실질간에 주로 분포하고 있었다. PP세포의 출편빈도는 십이지장부의 근위부와 중간부에서는 각각 42.5%, 55.4%로서 관찰된 반면 비장부의 모든 부위와 십이지장부의 원위부에서는 0.5%-2%로서 소수 출현되었다. This study was attempted to investigate the regional distribution and shapes of pancreatic endocrifle cells in the hedgehog, Erinaceus koneanus by the immunocytochemical PAP methods (Nalane, 1968; Stemberger, 1979). The tissue specimen taken from the splenic and duodenal regions of pancreas(proximai, middle and distal portions, respectively) were fixed with Bouin solution and the sections(5$\mu$ m) were followed by simple and double staining with 2 substrates, DAB and 4-CI-1-naphthol. The results were as following: Glucagon(A) cells, 13 $\mu$ m x 9.5 $\mu$ m in size, were found in the islets periphery and among the exocrine parenchyma. A cells were abundant in all the portions of splenic region and distal portion of duodenal region in contrast to a few in the proximal and middle portion of duodenal region. The shapes of the A cells were round, oval and pyramidal types. Insulin(B) cells, 11.6$\mu$m x 9.4$\mu$m in sise, were round or oval in shape and located throughout the islets. B cells were the most numerous cell types in all portions of splenic region and distal protion of duodenal region as compared with the other portions. Somatostatin(D) cells, 12.6$\mu$m x 9.1 $\mu$m in size, were round or oval in shape and found in the islets periphery and scattered in the exocrine parenchyma. These cells were rare in all the portions of splenic and duodenal region. Pancreatic polypeptide(PP) cells of various type, 12.8$\mu$ m x 8.5 $\mu$ m in size, were found in the islets periphery and among the exocrine parenchyma. PP cells were numerous in the proximal and middle portion of duodenal region, but rarely scattered in the other portions.
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