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문소은,석은지,하진아,양현원,윤보경,이민주 한국발생생물학회 2024 발생과 생식 Vol.28 No.1
Gonadotropin-releasing hormone (GnRH), a critical hormone produced in the hypothalamus, is essential for regulating reproductive processes. It has also been demonstrated the presence of GnRH and its receptors (GnRHR) in ovarian and uterine tissues, but little was known about the regulation mechanism of their expression in these organs and ovarian aging. Therefore, the aim of this study was to investigate the expression of GnRHR in the ovary and uterus of mice, particularly after high-dose gonadotropin treatments and in relation to aging. Quantitative realtime-PCR (qRT-PCR) revealed that pituitary gland had the highest GnRHR expression in both young and aged mice. In addition, liver expression was higher in young mice, whereas thymus expression was higher in aged mice. GnRHR mRNA was present in the ovaries of both young and aged mice but nearly undetectable in the uterus of aged mice. We next examined the expression of GnRHR in the ovary and uterus in response to high-dose administration of pregnant mare serum gonadotropin (PMSG). After PMSG administration, GnRH mRNA levels were significantly decreased in the ovary but increased in the uterus. The expression of GnRH mRNA in these organs showed opposite trends to that of GnRHR expression. These results suggest the involvement of GnRH in age-related reproductive decline and the potential effects of high-dose gonadotropin treatments on reproductive organ function.
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하진아,신정우,석은지,김수현,선소정,양현원 한국통합생물학회 2023 Animal cells and systems Vol.27 No.1
Estradiol (E2) and progesterone (P4) are essential sex steroid hormones that play critical roles in thepituitary gland and uterus. Recently, nesfatin-1, a polypeptide hormone that regulates appetiteand energy homeostasis in the hypothalamus, was found to be expressed in the pituitary glandand uterus. In this study, we aimed to investigate the relationship between these two steroidhormones and the expression and function of nesfatin-1 in the pituitary gland and uterus usingGH3 cells, a lacto-somatotroph cell line, and THESC cells, an endometrial stromal cell line. First,we verified the presence of nesfatin-1 and nesfatin-1 binding sites in GH3 and THESC cells. E2increased the mRNA expression of NUCB2, the gene encoding the nesfatin-1 protein, in GH3cells, while P4 had no significant effect. In THESC cells, NUCB2 mRNA expression was decreasedby E2 but increased by P4. In addition, nesfatin-1 significantly increased growth hormone (GH)and prolactin (PRL) mRNA expression in GH3 cells, and E2 enhanced this effect. In THESC cells,nesfatin-1 significantly increased the mRNA expression of insulin-like growth factor bindingprotein 1 (IGFBP1) and PRL, which are decidualization marker genes, and P4 further enhancedthis effect. These results suggest that nesfatin-1 may act as a local regulator of GH and PRLproduction in the pituitary gland and decidualization in the uterus, modulating its effects inresponse to E2 and P4.
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NUCB2/nesfatin-1 suppresses the acrosome reaction in sperm within the mouse epididymis
김수현,선소정,김민비,하진아,석은지,양현원 한국통합생물학회 2023 Animal cells and systems Vol.27 No.1
Nesfatin-1, a polypeptide hormone derived from the nucleobindin 2 (NUCB2) precursor protein, isknown to regulate appetite and energy metabolism. Recent studies have also shown that NUCB2/nesfatin-1 is expressed in the reproductive organs of mice. However, the expression and potentialrole of NUCB2/nesfatin-1 in the mouse epididymis remain unclear. Therefore, we investigated theexpression of NUCB2/nesfatin-1 in the mouse epididymis and its potential function. NUCB2/nesfatin-1 was detected in the epididymis by qRT-PCR and western blotting, and highexpression levels were observed in epididymal epithelial cells by immunohistochemical staining. Pregnant mare’s serum gonadotropin (PMSG) and human chorionic gonadotropin (hCG)injections significantly increased NUCB2/nesfatin-1 expression in the epididymis. Aftercastration, NUCB2/nesfatin-1 expression in the epididymis decreased, but was significantlyincreased by testosterone injection. Nesfatin-1-binding sites were found in the middle piece oftesticular sperm, but were scarcely detected in the sperm head. By contrast, nesfatin-1 bindingsites were identified on the sperm head within the epididymis. Furthermore, nesfatin-1treatment inhibited the acrosome reaction in epididymal sperm. These results suggest that thenesfatin-1 protein produced in the epididymis binds to nesfatin-1 binding sites on the spermhead and plays a role in suppressing the acrosome reaction before ejaculation.
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