매실 추출물을 함유한 기능성 음료 개발

배지현,김기진,김성미,이원재,이선장,Bae, Ji-Hyun,Kim, Ki-Jin,Kim, Sung-Mi,Lee, Won-Jae,Lee, Sun-Jang 한국식품과학회 2000 한국식품과학회지 Vol.32 No.3

본 연구에서는 매실 추출물을 0.5%, 5%, 10%, 15% 및 20% 함유한 매실음료를 제조하여 이들의 위암, 대장암, 간암 및 자궁암 세포주 증식에 미치는 항암효과를 MTT(3-[4,5-dimethylthiazol-2-yl]-2,5-diphenyltetrazolium bromide) assay, 세포독성 test 및 현미경 관찰 등을 통하여 조사해 보았다. 또한 12주간의 매실음료 섭취가 운동선수들의 체내 수분대사 조절 능력에 미치는 영향을 비교해 보았다. 20% 매실 추출물을 함유한 매실음료의 경우 위암 세포주인 SNU-16에 대해 증식억제 효과를 보였고, $1000\;{\mu}g/mL$ 농도에서는 유의적인 세포수의 감소를 관찰할 수 있었다. 매실음료를 배양액에 첨가하여 6일간 배양시켰을 때 SNU-16 세포주의 형태학적 변화를 볼 수 있었고 fibroblast에는 아무런 변화가 없었다. 매실음료를 섭취한 운동선수들의 경우 혈중 Na, K 및 Cl농도는 안정시에 비해서 운동 중 회복기에 유의하게(P<0.05) 증가하였으며 12 주간의 음료섭취 후 두 그룹 모두 거의 동일한 변화양상을 나타내었다. This study was performed to investigate the cytotoxic effect of the Prunus mume extracts containing beverages on the growth of SNU-16 gastric cancer cell, SNU-C2A colon cancer cell, SNU-449 liver cancer cell and HeLa cervical cancer cell. The inhibitory effect on the growth of the cancer cell lines was examined by MTT(3-[4,5-dimethylthiazol-2-yl]-2,5-diphenyltetrazolium bromide) assay, cytotoxicity test and microscopy. Also this study aimed to compare the changes of blood electrolytes and osmolarity during submaximal exercise for the intake of the Prunus mume extracts containing beverages. 20% Prunus mume extracts containing beverage exhibited the greatest inhibitory effect on the growth of SNU-16 and significantly inhibited at the concentration of $1000\;{\mu}g/mL$ in the MTT assay. Morphological changes in SNU-16 which treated with the same beverage were observed under inverted microscope. The change of blood electrolytes and osmolarity during submaximal exercise showed no significant differences between before and after intake of the beverage in both groups.

외상성 공막 천공 환자에서 시행한 자가테논낭이식 1예

배지현,성현경,김하경,남우호,Ji Hyun Bae,Hyun Kyoung Seong,Ha Kyoung Kim,Woo Ho Nam 대한안과학회 2008 대한안과학회지 Vol.49 No.10

Purpose: To report the use of autologous Tenon’s capsule graft for repair of scleral defects caused by traumatic scleral perforation. Case summary: An 81-year-old man presented with loss of vision in his right eye after a perforating injury caused by a cow horn. Examination showed a laceration of the sclera at 12 o’clock approximately 5~6 mm in length, and a uveal tissue was prolapsed into the wound. The best corrected visual acuity was 0.1. Primary repair of the eye was insufficient because of tissue loss. The inferonasal Tenon’s capsule graft was carefully dissected from the sclera and tailored to fit the defect. The graft was covered with a conjunctival flap. The scleral defect was successfully closed with the autologous Tenon’s capsule graft. Three months after grafting, phacoemulsification with intraocular lens implantation was performed. Conclusions: Autologous Tenon’s capsule graft is an effective measure to repair traumatic scleral defects and is useful when patch grafts are unexpectedly needed. J Korean Ophthalmol Soc 2008;49(10):1706-1710

교정나이 3세가 된 미숙아의 굴절이상, 약시 및 사시

배지현,최동규,Ji Hyun Bae,Dong Gyu Choi 대한안과학회 2010 대한안과학회지 Vol.51 No.10

Purpose: To evaluate the refractive status, anisometropia, amblyopia and strabismus in 3-year-old premature children. Methods: A total of 161 eyes from 82 premature infants were retrospectively reviewed and divided into three groups according to the presence of retinopathy of prematurity (ROP) and treatment; Group 1 (68 eyes): without ROP, Group 2-1 (32 eyes): spontaneously regressed ROP, and Group 2-2 (61 eyes): regressed ROP with treatment. The incidences and clinical features of refractive errors, anisometropia, amblyopia and strabismus at the age of three years were compared among the three groups. Results: The incidences of myopia, astigmatism, anisometropia and the severity of myopia increased according to the presence of ROP and treatment (p = 0.03, 0.02, 0.001, and 0.04, respectively). There were no significant differences in the incidences of hyperopia among the three groups; however, the severity of hyperopia in Group 2-2 was higher than those in the other two groups (p = 0.01). Patients in Group 1 had better best corrected visual acuity (BCVA) compared with those of the other two groups (p < 0.001). However, no significant differences in the incidences of strabismus or amblyopia among the three groups (p = 0.80 and 0.85, respectively) were found, and the ratio of esotropia : exotropia was 1:1.3. Conclusions: Regular ocular examination should be required in children who were born prematurely in order to detect and treat ophthalmologic problems such as refractive errors, amblyopia and strabismus. J Korean Ophthalmol Soc 2010;51(10):1385-1391

식중독 유발 세균의 증식에 미치는 겨우살이 추출물의 영향

배지현,노숙희,박효정,Bae Ji-Hyun,No Suck-Hee,Park Hyo-Jung 동아시아식생활학회 2005 동아시아식생활학회지 Vol.15 No.2

This study was performed to investigate the antimicrobial effect of the Viscum album var. coloratum extracts against food-borne pathogens. First, the Viscum album var. coloratum was extracted with methanol at room temperature and the fractionation of the methanol extracts was carried out by using petroleum ether, chloroform, and ethyl acetate, and methanol, respectively. The antimicrobial activity of the Viscum album var. coloratum extracts was determined by using a paper disc method against food-borne pathogens and food spoilage bacteria. The petroleum ether extracts of Viscum album var. coloratum showed the highest antimicrobial activity against Bacillus cereus and Shigella dysenteriae. Synergistic effect in inhibition was observed when Viscum album var. coloratum extract was mixed with Perillae folium extract as compared with each extract alone. Finally, the growth inhibition curves were determined by using petroleum ether extracts of Viscum album var. coloratum against Bacillus cereus and Shigella dysenteriae. The petroleum ether extract of Viscum album var. coloratum had strong antimicrobial activity against Bacillus cereus at the concentration of 5,000 ppm. At this concentration, the growth of Bacillus cereus was retarded more than 24 hours and up to 12 hours for Shigella dysenteriae. In conclusion, the petroleum ether extracts of Viscum album var. coloratum inhibit efficiently Bacillus cereus and Shigella dysenteriae.

햇빛유치원 교사들의 수업목표 설정과 적용에 관한 실행연구

배지현(Bae Jee-Hyun),이경화(Lee Kyung-Hwa) 한국영유아보육학회 2012 한국영유아보육학 Vol.0 No.70

본 연구의 목적은 교육목표 중 ‘수업목표’가 유아교육현장에서 어떻게 설정되고 수업에 적용되는지 알아보고, 신교육목표분류학을 매개로 수업목표에 대한 유아교사들의 반성적 실행과 수업개선을 도모하는데 있다. 햇빛유치원의 교사 5명을 대상으로, 6주간 실시한 연구의 결과를 살펴보면, 첫째, 유아교사들의 수업목표 설정방법은 ‘교육이론’, ‘발달수준’, ‘주제’, ‘시기’, ‘동료 교사’ 등을 복합적으로 고려한 방법이었다. 둘째, 교사들의 수업목표개선을 위한 실행과정은, ‘신교육목표분류학의 소개와 적용가능성’ 논의, ‘개선을 위한 적용의 시작’, ‘다양한 적용방법 개발과 적용’, ‘개선의 지속과 미래를 위한 종합’으로 나타났다. 본 연구의 시사점은, 수업목표에 대한 반성적 논의는 장학의 역할과 교사의 반성적 성장을 이끌어 낼 수 있다는 점, 수업목표의 역할에 대한 재고해볼 여지를 주었다는 점이다. The purpose of this study was to examine the ways of instructional goal setting, which belonged to educational objectives, among early childhood teachers and their methods of attaining selected goals. A new taxonomy of educational objectives was utilized to shed light on their reflective attainment of instructional goals and the process of their improvement. The findings of the study were as follows: First, as for the ways of instructional goal setting among the early childhood teachers in Sunshine Kindergarten, they set instructional goals in consideration of educational theories, the developmental level of preschoolers, the selected theme, time period and fellow teachers. And they reflected on their practices of setting goals without taking evaluation into account. Second, concerning the ways of improving instructional goals among the early childhood teachers, they had a discussion about the feasibility of a new taxonomy of educational objectives after they were explained about that. In the next stage of acting, the teachers started to apply it in accordance with the level of activities, to use questioning, to make a program evaluation and to offer differentiated guidance customized to the level of preschoolers. Thus, they got to set their instructional goals in a more productive manner. In the final stage of sustainable improvement and preparation for the future, they produced new evaluation tools by taking their selected goals into consideration, and carefully thought of the significance of the study in which they participated. The findings of the study had some implications: First, having a reflective discussion on instructional goals can serve as an opportunity of supervision for early childhood teachers. Second, this study provided diverse perspectives about the roles of instructional goals. Third, this study found that having a discussion on instructional goal setting and its attainment could serve as an opportunity for teachers to achieve a reflective growth.

식품 및 환경, 기타 물푸레 추출물의 식중독성 미생물에 대한 항균효과

배지현 ( Ji Hyun Bae ) 한국미생물생명공학회 2004 한국미생물·생명공학회지 Vol.32 No.3

쇠비름 추출물이 신생아 장염 유발 미생물에 미치는 영향

배지현 ( Ji Hyun Bae ) 한국식품영양학회 2012 韓國食品營養學會誌 Vol.25 No.2

본 연구에서는 신생아 질환과 사망 원인 중 첫 번째에 해당하는 설사의 주된 원인균인 C. jejuni와 기타 장내 염증 유발 유해 세균에 대한 쇠비름 추출물의 항균력을 검증하였다. 과거부터 민간이나 한방에서 널리 사용되어져 온 쇠비름은각종 항균 효과나 항염증, 항암 효과 등이 있는 것으로 알려져있다, 쇠비름을 각종 유기용매와 열수로 추출하여 Salmonellatyphimirium, Salmonella enteriditis, Shigella dysenteriae, Shigellaflexneri, Shigella sonnei 및 C. jejuni에 대한 항균활성을 조사해 본 바, 쇠비름의 ethylacetate 추출물 및 열수 추출물이 이들에 대한 항균력을 나타내었다. 쇠비름의 ethylacetate 추출물이 10 ㎎/㎖ 농도에서 Salmonella typhimurium에 대해 가장높은 항균력을 나타내었고, 열수 추출물도 유사한 항균성을 나타내었다, C, jejuni에 대한 쇠비름 열수 추출물의 MIC 범위는 10~20 ㎎/㎖였으며, MIC50은 10 ㎎/㎖, MIC90은 20 ㎎/㎖이었다, 쇠비름 열수 추출물은 10 ㎎/㎖ 이상의 농도에서C. jejuni에 대한 강한 항균력을 나타내 보였다. 또한 액체 배지에 배양한 C. jejuni에 대해 쇠비름의 열수 추출물이 10~20㎎/㎖ 농도에서 36시간까지 성장 억제 효과를 보였다. Diarrheal diseases constitute one of the major causes of morbidity and mortality in infants and young children globally. One of the main microorganisms causing diarrheal diseases is Campylobacter jejuni. For treatment of these diseases, Portulaca oleracea has been widely used as a folk remedy for a long time. This study was performed to investigate the antimicrobial activity of P. oleracea against gastroenteritis pathogens including C. jejuni. P. oleracea was extracted with petroleum ether, chloroform, ethylacetate, methanol, and hot water. The antimicrobial activity of the P. oleracea extracts was determined using the paper disc method, minimum inhibitory concentration, and the liquid culture method. The 10 ㎎/㎖ ethylacetate extract showed the strongest antimicrobial activity against Salmonella typhimurium, Salmonella enteriditis, and Shigella spp. The hot water extract from P. oleracea showed the highest anti-microbial activity against C. jejuni at 10~20㎎/㎖. The hot water extract of P. oleracea retarded the growth of C. jejuni for 36 hr at 42℃.

쇠비름 추출물 발효액이 Campylobacter jejuni의 증식에 미치는 영향

배지현 ( Ji Hyun Bae ) 한국식품영양학회 2012 韓國食品營養學會誌 Vol.25 No.2

본 연구에서는 신생아 질환과 사망 원인 중 첫 번째에 해당하는 설사의 주된 원인균인 Campylbactor jejuni에 대한 쇠비름 발효액의 항균 효과를 검증해 보고자 하였다. 민간요법과 한방에서 널리 이용되어온 쇠비름은 각종 항균 효과나 항염증, 항암 효과 등이 있는 것으로 알려져 있는 식용 식물로, 여기에서 식물성 유산균을 분리해 각종 프로바이오틱스와 더불어 쇠비름 발효액을 제조하였다. Lactobacillus rhamnosus, L.acidophilus, L. bulgaricus, L. delbrueckii, L. plantarum, Leuconostoc mesenteroides 및 Bifidobacterium longum과 쇠비름에서 분리한 균을 쇠비름 추출물에 적용하였을 때, Leuconostoc mesenteroides 와 쇠비름에서 분리한 식물성 유래 유산균이 쇠비름 추출액에서 가장 잘 자랐고, 여기에 2% yeast extract, 1% peptone 및 0.05~1% 인산을 첨가하였을 때 가장 적합한 발효액을 만들수 있었다. 쇠비름 발효액에 들어있는 생균수는 발효 48시간후 1×1012 CFU/㎖로 증가하였고, 2주간 냉장 보관을 한 이후에도 생균수가 1.3×1010 CFU/㎖로 남아있었다. 쇠비름 발효액의 pH와 산도는 발효 48시간 이후 3.7과 3.14로 각각 나타나 일반 유제품 발효액의 기준 범위 안에 들어감을 알 수 있었다. PLAB과 Leuconostoc mesenteroides로 만든 쇠비름 발효액은 10 ㎎/㎖ 이상의 농도에서 C. jejuni에 대해 증식 억제효과를 나타냈으며, 이 억제 효과는 48시간 동안 지속되었다. One of the main microorganisms causing diarrheal diseases is Campylobacter jejuni. Purslane or Portulaca oleracea is an edible plant containing polyphenols that has been widely used as a folk remedy for treatment of diarrhea for a long time. This study was performed to investigate the antimicrobial activity of fermented P. oleracea extracts made with probiotics and plant-origin lactic acid bacteria(PLAB) isolated from P. oleracea against C. jejuni. Lactobacillus rhamnosus, L. acidophilus, L. bulgaricus, L. delbrueckii, L. plantarum, Leuconostoc mesenteroides and Bifidobacterium longum were applied to P. oleracea to make a fermentation broth of purslane. Leuconostoc mesenteroides and the lactic acid bacteria isolated from P. oleracea grew best in the fermentation broth of P. oleracea extracts when the broth was combined with 2% yeast extract, 1% peptone, and 0.05 to 1% potassium phosphate. The number of viable cells in the fermentation broth containing purslane extracts after 48 hours increased to 1×1012 CFU/㎖ and remained at 1.3×1010 CFU/㎖ after refrigeration for 2 weeks. The pH and acidity of purslane-fermented broth after 48 hours of fermentation was 3.7 and 3.14, respectively, which show that the fermentation broth was within the range of the general standards of fermented dairy products. The antimicrobial activity of the fermented P. oleracea extracts was determined using the liquid culture method. The 10 ㎎/㎖ concentration of the fermented P. oleracea extract made with Leuconostoc mesenteroides and the lactic acid bacteria isolated from purslane showed the strongest antimicrobial activity against C. jejuni. The fermentation broth of purslane with the probiotics retarded the growth of C. jejuni for 48 hours at 42℃.

백두옹 추출물의 식중독성 미생물에 대한 항균효과

배지현(Bae Ji-Hyun) 韓國營養學會 2004 Journal of Nutrition and Health Vol.37 No.8

신교육목표분류학에 기초한 교사의 수업목표 인식과 설정에 따른 유아문학수업 개선 사례

배지현 ( Jee Hyun Bae ),이경화 ( Kyung Hwa Lee ) 한국어린이문학교육학회 2014 어린이문학교육연구 Vol.15 No.3

본 연구의 목적은 신교육목표분류학을 기초해 교사들의 수업목표 인식과 설정에 따른 유아문학수업에 관한 실행과정을 통해 수업 개선을 도모하는데 있다. 이를 위해 유아교사 3명을 대상으로, 16주간 실시한 연구 결과를 살펴보면, 첫째, 수업목표 인식과 설정에 따른 유아문학수업 개선에 관한 실행과정은 ‘계획하기: 수업목표 설정에 관한 반성적 점검과 개선방안 찾기’, ‘행동하기: 적용상의 어려움과 극복과정’, ‘관찰하기: 지속적 반성과 실행을 통한 수업 개선’, ‘반성하기: 지속적 미래를 위한 반성과 종합’을 거쳤다. 교사들은 목표 수준의 향상과 수업에의 반영, 달라진 발문들을 통해 종합적인 ‘유아문학수업개선을 위한 교사발문전략’ 다섯 가지를 정리하였다. 둘째, 수업목표 인식과 설정에 따른 유아문학수업 개선 사례의 특징은 ‘자기장학과 동료장학의 통합적 수업 개선’과 ‘신교육목표분류학의 유아문학교육적 활용’으로 나타났다. 연구의 시사점은 협력적 실행연구의 장학역할과 수업목표인식과 설정을 통한 다양한 문학수업에의 적용이 가능함을 보여준 것이다. The purpose of this study was to examine how kindergarten teachers offered literature lessons in consideration of their awareness and setting of instructional objectives according to the new taxonomy of educational objectives in an effort to step up the improvement of literature education for young children. The subjects in this study were three selected kindergarten teachers. This study was implemented for 16 weeks, and the findings of the study were as follows: First, the teachers tried to improve their literature classes in four stages based on their awareness and setting of instructional objectives. The first stage was planning, which was to check the selected instructional objectives from a reflective perspective and seek reform measures. The second was acting out, which was to address difficulties that they faced. The third was observing, which was to improve literature lessons through continuing reflection and acting out, and the fourth was reflecting, which was to keep reflecting and piece together what``s reflected in pursuit of sustainable development. The teachers formulated five teacher questioning strategies to improve their literature classes after they tried to raise the level of objectives, to reflect the selected objectives in their classes and to ask questions in a different way. Second, regarding the meanings of the efforts to improve early childhood literature classes based on awareness and setting of instructional objectives, one goal was to improve classes in an integrated manner and through self-supervision and peer supervision, and the other was to utilize the new taxonomy of educational objectives in early childhood education.