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Vehicular Ad-hoc Network 환경에서 IPFS 와 블록체인을 활용한 안전하고 효율적인 교통정보시스템
박한울 ( Hanwool Park ),허가빈 ( Gabin Heo ),도인실 ( Inshil Doh ) 한국정보처리학회 2022 한국정보처리학회 학술대회논문집 Vol.29 No.1
현재의 교통정보시스템은 수집된 정보를 서버에서 가공하여 서비스하는 형태로 이루어져 있다. 이러한 형태는 네트워크 구성이 비교적 단순하고 유지관리 비용이 적게 든다는 장점이 있지만, 반면에 실시간성이 저하되고 보안이 제대로 보장되지 않을 수 있다는 문제가 있으며, 최근 많은 연구가 이루어지고 있는 VANET 환경에서의 교통정보시스템도 broadcast storm 의 가능성을 안고 있다. 본 연구에서 제안하는 교통정보시스템은 자동차가 수집한 돌발 상황에 대한 데이터를 RSU(Road Side Unit)가 수신하고, 이후 메시지를 노드들에게 보낼 때 블록체인에 업로드함으로써 보안성과 broadcast storm 문제들을 해결할 수 있으며, raw data 를 IPFS 에 저장하여 시스템고도화에 사용할 수 있어 참여자들이 교통 상황에 대해 신속하게 대응할 수 있도록 하는 장점을 갖는다.
미세조류 Chlamydomonas reinhardtii dZL 균주의 100 L 평판형광생물 반응기를 이용한 대량 배양에서의 접종 농도에 따른 바이오매스 생산성 향상 연구
박한울(Hanwool Park),강성모(Sung-Mo Kang),민지호(Ji-Ho Min),김준호(Jun-Ho Kim),조용희(Yonghee Cho),김기현(Ki-Hyun Kim),진언선(Eonseon Jin),홍성주(Seong-Joo Hong),이철균(Choul-Gyun Lee) 한국생물공학회 2020 KSBB Journal Vol.35 No.1
Beside the commercialized pigments from microalgae, such as astaxanthin, beta-carotene, and c-phycocyanin, zeaxanthin is another pigment with great potential for commercialization as it can be used to prevent or cure age-related macular degeneration. However, low zeaxanthin productivity due to its low content in microalgal biomass has been the major obstacle to commercialization. With recent advances in genome editing technology, a green microalga Chlamydomonas reinhardtii dZL was engineered to accumulate zeaxanthin with growth by knocking out enzymes that convert zeaxanthin into other carotenoids. In the present study, C. reinhardtii dZL was cultivated using TAP medium in 100 L flat-panel photobioreactors (PBRs), and the effect of initial cell density(ICD) on biomass productivity in batch operation was investigated. ICD of 0.1 g/L was predicted to yield highest biomass productivity from experiments using 2 L bubble column PBRs. In 100 L flat-panel PBRs, as ICD increased from 0.005 g/L to 0.08 g/L, biomass productivity was increased from 0.03 g/L/ day to 0.16 g/L/day. However, further increasing ICD from 0.08 g/L to 0.16 g/L reduced to 0.14 g/L/day as the maximum biomass concentration was limited by the light intensity. The results showed that ICD could affect biomass productivity in positive and negative ways, and thus ICD should carefully be determined with regards to the culture conditions. Such strategy could be applied to improve productivity for other compounds of interests in other microalgae as well.
신규 분리된 담수미세조류 Parachlorella sp.의 지방산 생산성 향상을 위한 배지 조성 연구
박한울 ( Hanwool Park ),임경준 ( Kyung June Yim ),민지호 ( Ji-ho Min ),강성모 ( Sung-mo Kang ),한찬우 ( Chan-woo Han ),이창수 ( Chang-soo Lee ),정지영 ( Ji Young Jung ),홍성주 ( Seong-joo Hong ),이철균 ( Choul-gyun Lee ),김지훈 ( 한국미생물 · 생명공학회 2020 한국미생물·생명공학회지 Vol.48 No.3
본 연구에서는 국내 낙동강 수계에서 신규하게 분리된 미세조류인 Parachlorella sp. 종의 바이오매스 및 지방산 생산성에 대한 배지의 영향을 연구하였다. 미세조류 배양에 통상적으로 사용되는 BG-11, TAP, BBM 배지를 사용하여 바이오매스 생산성은 TAP 배지에서, 지방산 축적은 BBM 배지에서 가장 잘 일어나는 것으로 확인되었고, 지방산 생산성을 향상시키기 위해 암모니아와 아세트산을 사용하는 TAP 배지의 조성을 변화하여 BBM 배지처럼 지방산 축적을 유도하며 바이오매스 생산성을 증가시킨 MTAP 배지를 개발하였다. 전체적인 바이오매스와 지방산 생산성을 높이기 위해서는 MTAP-1 배지가 적합하여 바이오매스 생산성과 지방산 생산성은 기존의 TAP 배지 대비 각각 14%, 45% 증가하였다. 생리 활성 효과로 인해 관심도가 높은 오메가-3 지방산의 생산에는 MTAP-4 배지가 가장 적합하여 바이오매스 생산성과 오메가-3 지방산 생산성이 기존 BBM 배지 대비 각각 18%, 39% 증가하여 목표 중점 생산물질(바이오매스, 총 지방산, 또는 오메가-3 지방산)의 생산성을 향상시킬 수 있는 신규 배지 2종의 조성을 개발하였다. Parachlorella sp. is an efficient fatty acid producer that can be used in the production of biofuels, feeds, and fertilizers. Microalgae show varying responses to culture conditions, even those within the same species. In this study, growth and fatty acid composition of a newly isolated Parachlorella sp. from the Nakdong river of Korea in different culture media were investigated. The microalga was cultivated in 400 ml bubble column photobioreactors using BG-11, BBM, TAP, and modified TAP (MTAP) media. It was shown that using BBM led to greater fatty acid accumulation (34%), while using TAP medium led to greater biomass productivity (0.34 g/l/day). Composition of the TAP medium was modified to have the N:P ratio of BBM while also varying concentrations of N and P to improve fatty acid productivity. One of the modified TAP media, MTAP-1 (104.8 mgN/l, 135.2 mgP/l, N:P ratio = 0.77), showed the highest fatty acid concentration of 0.69 ± 0.04 g/l, while those from TAP and BBM were 0.48 ± 0.06 g/l and 0.40 ± 0.02 g/l, respectively. The results showed that microalgal fatty acid productivity could be enhanced by changing the N:P ratio and concentrations.
저온 생장성이 우수한 분리 미세조류 Tetraselmis sp. 5개주의 생장 패턴 및 지방산 조성 분석
박한울(Hanwool Park),허동희(Donghee Hoh),신동우(Dong-Woo Shin),김지훈(Z-Hun Kim),홍성주(Seong-Joo Hong),임상민(Sang-Min Lim),이철균(Choul-Gyun Lee) 한국해양바이오학회 2019 한국해양바이오학회지 Vol.11 No.1
For successful microalgal biodiesel production, the strain should be selected carefully. Fast growth rate and high fatty acid contents are desired traits for algal biodiesel production. In ocean cultivation of microalgae, seawater temperature slowly changes over seasons, and rotating algal strains in accordance with their optimal temperature could improve overall productivity. Additionally, use of indigenous strain is preferred to alleviate potential impacts on the environment. In this study, five strains of Tetraselmis sp. from nearshore of Youngheung Island, Incheon, Korea, were isolated during winter and characterized for their growth patterns and fatty acid compositions in the low temperatures (5–15°C). The five strains showed various characteristics in optimal growth temperature, fatty acid contents, and compositions. Compared with a strain of Tetraselmis sp., isolated from Ganghwa island in a previous study, a rapid-growing strain with 237% higher biomass productivity and an oleaginous strain with twice higher fatty acid contents at 10°C were isolated. The oleaginous Tetraselmis strain showed the highest fatty acid productivity among the strains, having 438% higher productivity than the previous strain. Using the new isolates in the seasons with low seawater temperature would improve microalgal fatty acid productivity in ocean cultivation.
플레어 팁 축소 모형을 이용한 Flaring 소음 연구
박성종(Seongjong Park),김종원(Jongwon Kim),김한울(Hanwool Kim),이재훈(Jaehoon Lee),임효남(Hyo-Nam Im),이희성(Heesung Lee),이동연(Dongyeon Lee) 대한기계학회 2020 대한기계학회 춘추학술대회 Vol.2020 No.12
Flare system on offshore production facilities is a safety device for burning and discharging residual gas from oil or gas wells. When flaring occurs, gas is burnt off combining with air on the flare tips, which releases high noise to the surroundings. Therefore, flaring noise has to be strictly controlled to protect workers hearing and countermeasures against the noise are taken into account at the design stage. In general, the study of flaring noise effect on working environment has been dependent on noise calculation or the data provided by flare tip manufacturers. However, the reliability of the noise data is insufficient to take appropriate measures and it is difficult to verify the noise data due to the lack of experience and database. In this study, in order to assess the characteristics of flaring noise, a series of combustion experiments has been carried out using flare tip scale models. Flaring noise are measured at steady-state with intervals of 5.0 m from the center of flare tips with varying the shape, size and gap of the tip nozzle and gas flow rate. In addition, the measured flaring noise levels are compared to calculated results from the commercial software.
부유형 해양 광생물반응기를 이용한 자외선과 초기 미세조류 접종 농도와의 상관관계 규명
김지훈,박한울,정성균,김수권,김희윤,박용성,홍한마루,이철균,Kim, Z-Hun,Park, Hanwool,Jung, Seong-Gyun,Kim, Su-Kwon,Kim, Hee-Yun,Park, Yong Sung,Hong, Han Ma Roo,Lee, Choul-Gyun 한국해양바이오학회 2015 한국해양바이오학회지 Vol.7 No.2
The purpose of this study was to investigate a relationship between ultraviolet radiation and initial cell density (ICD) of microalgae using a floating marine photobioreactor (PBR). To examine the effect of ultraviolet (UV) radiation in sunlight on biomass productivity as a function of ICD, 0.5-L floating PBRs covered with or without UV cut-off film were placed in an outdoor rectangular tank containing 200 L of water. At the lower ICDs, 0.01 and 0.05 g/L, biomass productivities in the PBRs without UV cut-off film decreased by $278{\pm}21%$ and $222{\pm}3%$ compared with those with the film, respectively. In contrast, the presence of UV cut-off film did not have a significant effect on biomass productivities at the higher ICDs, 0.25 and 1.25 g/L. When the differences in biomass productivity made by the UV cut-off film were plotted against the sum of cell projection area per light receiving area of the PBR, the results revealed that the inhibitory effect of UV on biomass productivity can be negligible when the sum of cell projection area is equal to the light receiving area of the PBR. These results show that photoinhibition caused by UV radiation could be eliminated via operating the PBR with a proper ICD.