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신규 분리된 담수미세조류 Parachlorella sp.의 지방산 생산성 향상을 위한 배지 조성 연구
박한울 ( Hanwool Park ),임경준 ( Kyung June Yim ),민지호 ( Ji-ho Min ),강성모 ( Sung-mo Kang ),한찬우 ( Chan-woo Han ),이창수 ( Chang-soo Lee ),정지영 ( Ji Young Jung ),홍성주 ( Seong-joo Hong ),이철균 ( Choul-gyun Lee ),김지훈 ( 한국미생물 · 생명공학회 2020 한국미생물·생명공학회지 Vol.48 No.3
본 연구에서는 국내 낙동강 수계에서 신규하게 분리된 미세조류인 Parachlorella sp. 종의 바이오매스 및 지방산 생산성에 대한 배지의 영향을 연구하였다. 미세조류 배양에 통상적으로 사용되는 BG-11, TAP, BBM 배지를 사용하여 바이오매스 생산성은 TAP 배지에서, 지방산 축적은 BBM 배지에서 가장 잘 일어나는 것으로 확인되었고, 지방산 생산성을 향상시키기 위해 암모니아와 아세트산을 사용하는 TAP 배지의 조성을 변화하여 BBM 배지처럼 지방산 축적을 유도하며 바이오매스 생산성을 증가시킨 MTAP 배지를 개발하였다. 전체적인 바이오매스와 지방산 생산성을 높이기 위해서는 MTAP-1 배지가 적합하여 바이오매스 생산성과 지방산 생산성은 기존의 TAP 배지 대비 각각 14%, 45% 증가하였다. 생리 활성 효과로 인해 관심도가 높은 오메가-3 지방산의 생산에는 MTAP-4 배지가 가장 적합하여 바이오매스 생산성과 오메가-3 지방산 생산성이 기존 BBM 배지 대비 각각 18%, 39% 증가하여 목표 중점 생산물질(바이오매스, 총 지방산, 또는 오메가-3 지방산)의 생산성을 향상시킬 수 있는 신규 배지 2종의 조성을 개발하였다. Parachlorella sp. is an efficient fatty acid producer that can be used in the production of biofuels, feeds, and fertilizers. Microalgae show varying responses to culture conditions, even those within the same species. In this study, growth and fatty acid composition of a newly isolated Parachlorella sp. from the Nakdong river of Korea in different culture media were investigated. The microalga was cultivated in 400 ml bubble column photobioreactors using BG-11, BBM, TAP, and modified TAP (MTAP) media. It was shown that using BBM led to greater fatty acid accumulation (34%), while using TAP medium led to greater biomass productivity (0.34 g/l/day). Composition of the TAP medium was modified to have the N:P ratio of BBM while also varying concentrations of N and P to improve fatty acid productivity. One of the modified TAP media, MTAP-1 (104.8 mgN/l, 135.2 mgP/l, N:P ratio = 0.77), showed the highest fatty acid concentration of 0.69 ± 0.04 g/l, while those from TAP and BBM were 0.48 ± 0.06 g/l and 0.40 ± 0.02 g/l, respectively. The results showed that microalgal fatty acid productivity could be enhanced by changing the N:P ratio and concentrations.
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학생의 체질, 체력변화에 관한 종단적 조사 분석 : 초, 중, 고 학생을중심으로
김대건,한찬우 東國大學校 敎育硏究院 1997 교육문제연구 Vol.12 No.-
This study, the status of physique development, physical condition, physical strength by years, ages, and sexs, provided by the department of school physical education, local support bureau, ministry of education resulted from physique for 10 years from 1995 to 1995, change status of a physical condition and a physical strength for 10 years from 1985 to 1995 with the object of 100,580 students in total of 450 sample school for 10 years based on the result of physique, physical condition and six items of physical charter investigated by above mentioned authorities with the view of the examing the change of physique, physical condition, physical strength for the students' between the age of through 17 of the county wide elementary, middle, and high school vertically. 1. The change of the of physical condition A number of students' weak sight increased from 1.47% in 1985 to 3.40% in 1995 or 2.3 times. This means that it has increased 2.3 times compared with 10 years. Refracting deficiency increased from 8.16% in 1985 to 21.49% in 1995. This indicated that it has abruptly increased 2.6 times as many as 10 years period. As the result of 1995 investigation 3.4% of the country wide students' for 0.2 to 0.4% were proved to have the weak sight and 21.49% students' for 0.3% to 0.6% of the whole students', on top of these other students' proved to have the refracting deficiency including long sight and astigmatism students. Students' sight weakenization was more serious with the height of school years. The study was indication that about 13 students wore glasses in the case of the elementary school students', 32 students', and 32 in the case of the middle school and 42 of 100 students' in the case of the high school students' it was necessary for than to remedy their eye sight. The statistics of deceases about nose and neck including emphysema and the swollening of tonsil increased a little from 21% to 2.75% compared with 10 years ago. The study also showed diseases including caries increased a little to 51.2% from 50.06% in 1980. 1 can conclude the physical condition for students' continuesly go wrong. 2. The aspect of physical strength Change The change for the worse of all the events of students' over all the sports events shows the gradual of for all students'. Compared with 10 years before l00m running the male average record decreased 0.38sec, while it decreased to 0.71sec in the case of the female students', male students' pule-up decreased to 1.52 times and the record for female students' decreased to 2.91sec. The record of Hand throwing in the case of the male students' decreased 01.9m while in he case of female students' decreased to 1.66m and only the sit-up for the students' indicated the smiler number of it exceptionaly, However this part also indicated inferity compared with Ole record one year before. Standing broad jump indicated 3.16m Hl the case of male students' and 9.7cm decreas respectively in the case of female students' The physical measurement fields the list showing remarkable difference was the distance run. It's difference showed 24sec in the case of male students' and 25.Isec in the case of female students, compared with 10 years. This is the indication that the sustained power of adolescent's heart and lungs remarkably decreased.
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