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신규 분리된 Mychonastes sp.의 생장, 지방산 및 색소 생산에 생장배지가 미치는 영향
임경준(Kyung June Yim),장현진(Hyun-Jin Jang),박예지(Yeji Park),남승원(Seung Won Nam),황병수(Byung Su Hwang),정지영(Ji Young Jung),이창수(Chang Soo Lee),김지훈(Z-Hun Kim) 한국해양바이오학회 2022 한국해양바이오학회지 Vol.14 No.1
This study examined the growth, fatty acid (FA) content, and carotenoids of a newly isolated freshwater microalga, Mychonastes sp. 246, in various culture media. The appropriate temperature and light intensity for culturing Mychonastes sp. 246 were determined as 18°C–22°C and 200–250 μmol/m2/s using a high throughput photobioreactor. The microalgal cells were cultivated in 0.5 L bubble column photobioreactors using BG11, Bold’s Basal media, and f/2 media. According to the growth results of the microalgae, BG11, among the tested media, showed the highest biomass concentrations (3.5 ± 0.1 g/L in 10 d). To enhance the biomass growth of the microalgae, the N:P ratio in BG11 was manipulated from 45:1 to 7:1 based on the stoichiometric cell composition. The biomass concentrations of Mychonastes sp. 246 grown on the manipulated BG11 (MBG) increased to 38% (4.6 ± 0.3 g/L in d) compared with the original BG11 (3.3 g/L). The FA content of the microalgae grown on the MBG was lower (8.4%) than that of the original BG11 (10.1%) while the FA compositions did not exhibit any significant differences. Furthermore, three kinds of carotenoids were identified in Mychonastes sp. 246, zeaxanthin, lutein, and β-carotene. These results suggest an effective strategy for increasing biomass concentrations, FA content, and carotenoids of microalgae by performing a simple N:P adjustment in the culture media.
장현진(Hyun-Jin Jang),임경준(Kyung June Yim),조복연(Bok Yeon Jo),남승원(Seung Won Nam),남영호(Young Ho Nam),황병수(Byung Su Hwang),이창수(Chang Soo Lee),정지영(Ji Young Jung),우성환(Sung Hwan Woo),이순(Soon Lee),홍은미(Eunmi Hong),김 한국생물공학회 2021 KSBB Journal Vol.36 No.2
In this study, antioxidant and anticancer activities of methanolic extracts from newly isolated freshwater microalgae were investigated. First, four microalgal strains in Korea were identified as Mychonastes sp. 246, Mychonastes sp. 247, Mychonastes pushpae 248, and Mychonastes sp. 249 through 5.8S-ITS2 gene sequencing and their carotenoid contents were analyzed. Then, we accessed antioxidant, cytotoxic, and anticancer activities of microalgal methanolic extracts. We found that the genus Mychonastes contained 226~2,607 μg/g, 138~1,594 μg/g, and 126~339 μg/g of β-carotene, lutein, and zeaxanthin, respectively. All of the microalgal extracts showed increased antioxidant and anticancer activities in a dose-dependent manner. Antioxidant activities of the extracts were 31 ± 7%, 27 ± 8%, 23 ± 7%, and 17 ± 8%, respectively, when treated with 50 mg/mL. From the results of cytotoxicity test, we did not find any serious growth inhibition of human mesenchymal stem cells treated at 10 μg/mL to 200 μg/mL. In addition, the microalgal extracts could effectively inhibit growth of all tested A549, BxPC-3, HepG2, and SKOV-3 cancer cells ranged from 16% to 62% when treated with 200 μg/mL. These results indicate the methanolic extract of four strain of genus Mychonastes could be used as a potential natural material as pharmaceutical ingredients.
신규 분리된 담수미세조류 Parachlorella sp.의 지방산 생산성 향상을 위한 배지 조성 연구
박한울 ( Hanwool Park ),임경준 ( Kyung June Yim ),민지호 ( Ji-ho Min ),강성모 ( Sung-mo Kang ),한찬우 ( Chan-woo Han ),이창수 ( Chang-soo Lee ),정지영 ( Ji Young Jung ),홍성주 ( Seong-joo Hong ),이철균 ( Choul-gyun Lee ),김지훈 ( 한국미생물 · 생명공학회 2020 한국미생물·생명공학회지 Vol.48 No.3
본 연구에서는 국내 낙동강 수계에서 신규하게 분리된 미세조류인 Parachlorella sp. 종의 바이오매스 및 지방산 생산성에 대한 배지의 영향을 연구하였다. 미세조류 배양에 통상적으로 사용되는 BG-11, TAP, BBM 배지를 사용하여 바이오매스 생산성은 TAP 배지에서, 지방산 축적은 BBM 배지에서 가장 잘 일어나는 것으로 확인되었고, 지방산 생산성을 향상시키기 위해 암모니아와 아세트산을 사용하는 TAP 배지의 조성을 변화하여 BBM 배지처럼 지방산 축적을 유도하며 바이오매스 생산성을 증가시킨 MTAP 배지를 개발하였다. 전체적인 바이오매스와 지방산 생산성을 높이기 위해서는 MTAP-1 배지가 적합하여 바이오매스 생산성과 지방산 생산성은 기존의 TAP 배지 대비 각각 14%, 45% 증가하였다. 생리 활성 효과로 인해 관심도가 높은 오메가-3 지방산의 생산에는 MTAP-4 배지가 가장 적합하여 바이오매스 생산성과 오메가-3 지방산 생산성이 기존 BBM 배지 대비 각각 18%, 39% 증가하여 목표 중점 생산물질(바이오매스, 총 지방산, 또는 오메가-3 지방산)의 생산성을 향상시킬 수 있는 신규 배지 2종의 조성을 개발하였다. Parachlorella sp. is an efficient fatty acid producer that can be used in the production of biofuels, feeds, and fertilizers. Microalgae show varying responses to culture conditions, even those within the same species. In this study, growth and fatty acid composition of a newly isolated Parachlorella sp. from the Nakdong river of Korea in different culture media were investigated. The microalga was cultivated in 400 ml bubble column photobioreactors using BG-11, BBM, TAP, and modified TAP (MTAP) media. It was shown that using BBM led to greater fatty acid accumulation (34%), while using TAP medium led to greater biomass productivity (0.34 g/l/day). Composition of the TAP medium was modified to have the N:P ratio of BBM while also varying concentrations of N and P to improve fatty acid productivity. One of the modified TAP media, MTAP-1 (104.8 mgN/l, 135.2 mgP/l, N:P ratio = 0.77), showed the highest fatty acid concentration of 0.69 ± 0.04 g/l, while those from TAP and BBM were 0.48 ± 0.06 g/l and 0.40 ± 0.02 g/l, respectively. The results showed that microalgal fatty acid productivity could be enhanced by changing the N:P ratio and concentrations.