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박정호,Jin-Ho Park,Wonho Choi,Byung-Kwan Lim 대한독성 유전단백체 학회 2019 Molecular & cellular toxicology Vol.15 No.4
Backgrounds: Coxsackievirus B3 (CVB3) is a member of the family Picornaviridae, and along with polioviruses, belongs to the Enterovirus genus. The CVB3 genome is composed single-stranded RNA encoding polyproteins, which are cleaved to individual functional proteins by 2A and 3C proteases proteins which have been targeted for drug development. Here, we showed that protease activity required to activate a toxic protein may be used to prevent viral infection. Methods: We modified the MazE-MazF antitoxin-toxin system of Escherichia coli to fuse a C-terminal fragment of MazE to the N-terminal end of toxin MazF with a linker having a specific protease cleavage site for CVB3. This fusion protein formed a stable dimer and was capable of inactivating the mRNA interferase activity of MazF which cleaves the ACA sequence in mRNA substrates. Results: The incubation of 2A proteases with the fusion proteins induced cleavage between the MazE and MazF fragments from the fusion proteins; the subsequent release of MazF significantly inhibited virus replication. Additionally, we note that, CVB3 infected HeLa cells quickly died through a MazF toxin mediated effect before virus protein expression. Conclusion: These findings suggest that the MazEF fusion protein has a strong potential to be developed as an anti-virus therapy following CVB3 infection.
중성자 발생용 구형 집속빔 핵융합 장치의 방전현상 연구
박정호,주흥진,고광철,Park, Jeong-Ho,Ju, Heung-Jin,Ko, Kwang-Cheol 한국전기전자재료학회 2007 전기전자재료학회논문지 Vol.20 No.5
Application field of neutron beam is very broad including industry, medicine and science. But the research and development and use of neutron beam is restricted within in narrow limits in this country, because neutron beam facility is insufficient - a big research facility of nuclear reactor(HANARO) and some small industrial facilities which use radioisotope neutron source are available. This paper compare and investigate the results of experiment and numerical analysis of the discharge in the spherically convergent beam fusion device which were expected as a portable neutron source. The spherically convergent beam fusion device will offer stability in neutron production, possibility of movement for convenience, low construction cost and higher neutron flux than radioisotope neutron source. The star mode discharge which efficiently generate neutron, were observed at both results.