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박송아 심미안 2021 문학들 Vol.- No.65

B16F10 피부암 세포에서 AMPK-VASP 신호경로를 경유한 셀레늄의 Apoptosis 효과

박송이,Sol Hwa Lee,박옥진,김영민 대한암예방학회 2010 Journal of cancer prevention Vol.15 No.4

A vasodilatator-stimulierte phosphoprotein (VASP), Ena/VASP family, is connected to actin. It is associated with the signal pathway involving the proliferation and migration of cells, and VASP regulates cells-cells contacts. VASP in the blood acts as cAMP-and cGMP-dependent protein kinase. According to the positions of amino acid at Ser-157, Ser-239 and Thr-278, it is regulated by AMP-activated protein kinase (AMPK) that acts as Ser-Thr kinase. Recently the VASP is known to the new substrate of AMPK. The AMPK plays a role of the energy sensor. If AMPK is activated in cancer cells, apoptosis is induced in them. However, activation of mTOR is known to be promoted cancer growth. In addition, Akt, which is a kind of Ser-Thr kinase, is responsible for proliferation, differentiation, and growth of cells, and it inhibits apoptosis in cancer cells and is known to engage in angiogenesis and metastasis. In this study,when selenium is treated in B16F10 cancer cells, we suggest that the growth of B16F10 cells is inhibited. We determined with MTT assay, Fluorescence-Activated Cell Sorting (FACS), and Western blotting. Finally, it is observed whether these inhibitory effects is due to induction of apoptosis, and we have tried to evaluate the anti-tumor effect of selenium, through VASP, AMPK, mTOR and Akt signal pathway. (Cancer Prev Res 15, 313-319, 2010)

탄소나노튜브 혼입 전도성 건설재료의 저항변화율 추정을 위한 기계학습 기반 모델

박송이(Park, Song Ee),양범주(Yang, Beomjoo) 대한토목학회 2020 대한토목학회 학술대회 Vol.2020 No.10

자아 성장과 맞춤형의 멋진 신세계 이면 - 1인가구의 노동, 관계, 가족 영역에서 보이는 신자유주의 통치성의 그림자

박송이,김수영 비판사회학회 2024 경제와 사회 Vol.- No.141

The number of single-person households has expanded globally. In particular, South Korea has witnessed a dramatic rise in the percentage of single-person households among all households since 2000, with figures increasing from 20.0% in 2005 to 27.2% in 2015, 31.7% in 2020 and further to 34.5%. Researchers have sparked various debates on the shift from the traditional nuclear family system to an individualized family. The main point of contention is whether individual choose to live alone as positive alternative household or whether the single-person household is a negative result of social isolation or inequality. Although they seem to be making confliciting claims, researchers miss the political economy structure and are unable to explain why individuals act according to the neoliberal order by focusing only on fragmentary side. Based on these limitations, this study analyzed the interviews of 21 single-person households in the field of labor, human relations, and family by theory of neoliberal governmentality. The results are as follows. First, in the neoliberal govermentality that appears in the field of labor, single-person households perceived ubiquitous pressure to work constantly, and they were internalizing a sense of responsibility to grow oneself. However, behind the subjectification of being immersed in self-growth, anxiety about failure was laid. Second, in the neoliberal governmentality that appears in the field of human relations, participants were too immersed in self-growth, blocking relationships in the real life, sitting on the fence while just watching people, and attempting to establish optimized relationship but feeling emotional emptiness. Finally, in the neoliberal governmentality that appears in the field of family, they felt the burden of family formation, and paradoxically have a desire to establish an optimized family at the same time. However, because there were no clear alternatives to solve loneliness within this ambivalence, they just managed loneliness through family of origin and felt vagueness and loneliness about unmanageable family building. This study interprets the current situation that single-person households are vigorously investing themselves in self-growth, but losing the realm of relationships that can serve as another source of life for them, transforming Korean society like Aldous Huxley’s “Brave New World.” Therefore, the research suggests that public discourse and institutional experiments are needed to form a political economic structure that can establish authentic relationships.

Curcumin Induces Apoptosis by the Regulation of AMPKα1 and HIF-1α Levels

박송이,김윤이,이윤경,김영민,박옥진 대한암예방학회 2012 Journal of cancer prevention Vol.17 No.2

Under the hypoxic condition, cancer cells initiate their own adaptive pathways to have a facilitated oxygen and energy supply for survival. In this adaptive response, hypoxia-inducible factor-1α (HIF-1α) is a key regulator to stimulate angiogenesis, glucose transport and metabolism. We applied curcumin to examine the effect on HIF-1α induction and the regulatory effect of AMPKα1 on HIF-1α in HT-29 colon cancer cells. Our results showed that curcumin effectively induced apoptosis, dose dependently and reduced HIF-1α, which was elevated in hypoxic state induced by CoCl2. In addition, curcumin inhibited AMPKα1 activity, a modulator of adaptive pathway in response to hypoxia-induced energy depletion. These observations suggest that AMPKα1 may positively modulate HIF-1α stabilization and therefore, curcumin down-regulates AMPKα1 which seldom occurs in normoxic state. Curcumin induces apoptosis of HT-29 colon cancer cells through regulation of AMPKα1- HIF-1α pathway against hypoxic state.

최승자 시의 비극의식

박송이 한남대학교 한남어문학회 2007 한남어문학 Vol.31 No.-

MCF-7 유방암세포에서 Resveratrol과 Quercetin의 병행처리에 의한 AMPK 활성 및 시너지 효과

박송이,박옥진,김영민,이윤경 대한암예방학회 2009 Journal of cancer prevention Vol.14 No.3

pathway in many cancer cells. Resveratrol and quercetin have been known to exert anti-inflammatory or anti-cancer effects through regulating several pathways. AMP-activated protein kinase (AMPK) is one of the most important protein kinases involved in signal pathways related to metabolic control. AMPK is regulated by AMP/ATP ratio and activated by ATP consumption. Recently, it has been reported that AMPK is involved in the regulation of proliferation and/or apoptosis in several cancer cells. Our study investigated the synergistic effects of resveratrol and quercetin on apoptosis and AMPK activation in MCF-7 breast cancer cells. Treatment with resveratrol and quercetin or co-treatment with resveratrol and quercetin for 24 h inhibited cancer cell proliferation, and increased apoptotic cell death. The synergistic effects of resveratrol and quercetin were observed both in the inhibition of cell growth and the acceleration of apoptotic cell death. Also, the combined treatment of resveratrol and quercetin more strongly activated AMPK, and this effect was abolished by AMPK inhibitor Compound C in MCF-7 breast cancer cells. Treatment of AICAR, synthetic AMPK activator also increased phospho-AMPK levels. Our results suggest that combined treatment of resveratrol and quercetin is a more effective way in controlling cancer proliferation and apoptosis. (Cancer Prev Res 14, 218-224, 2009)

Regulation of AMP-activated Kinase and Cyclooxygenase-2 by Estrogen in Non-cancerous, Tumor-promoter Stimulated Non-cancerous or Breast Cancer Cells

박송이,이원섭,박옥진,이윤경,김영민 대한암예방학회 2009 Journal of cancer prevention Vol.14 No.2

Estrogen has been suggested as a promoter of breast cancer for its cell proliferative effect and antiapoptotic properties, and moreover, recent epidemiological data suggest that females are at higher risk for lung cancers than males, and it is attributed to the greater exposure to the genotoxicity of estrogen metabolites in the form of hydroxyestradiols. Our previous studies have revealed that AMP-activated protein kinase (AMPK) activation is strongly related to the inhibition of cancer cell growth. We examined the regulation of AMPK and cyclooxygenase-2 (COX-2) by exogenous estrogen in non-cancerous normal cells, TPA (12-O-tetradecanoylphorbol-13-acetate)-treated normal cells and MCF-7 breast cancer cells to elucidate the effects of estrogen treatment on AMPK and COX-2. The exogenous estrogen strongly stimulated COX-2 expression in TPA treated cox-2 +/+ cells and MCF-7 cells, but such stimulation was not observed with non-TPA treated normal cells. Estrogen activated AMPK in all three kinds of cells, and in TPA-treated and cancerous cells the activation of AMPK with estrogen could not abolish COX-2 expression. These results indicate that the cell type is an important defining factor of AMPK and COX-2 regulation by estrogen, and this may have some significance to unravel the possible link between estrogen and cancers.

다양한 백색 LED 광과 원적색광 보광에 의한 이고들빼기의 생산성 향상

박송이(Song-Yi Park),오명민(Myung-Min Oh) (사)한국생물환경조절학회 2021 생물환경조절학회지 Vol.30 No.2

인공광 이용형 식물공장에서 인공광원의 전력 소모는 작물생산 비용을 증가시키는 주요한 요인이다. 따라서 인공광원의 광 이용효율을 향상시키는 것은 식물공장의 경제성 확보에 중요한 사안이다. 본 연구의 목적은 다양한 스펙트럼을 갖는 백색 LED와 원적색광을 추가한 다양한 백색 LED에서 재배된 이고들빼기의 생산성과 광 이용효율을 비교하는 것이다. 파종 후 3주된 이고들빼기 묘는 심지 이용 수경재배 시스템에 정식되었고, 기온 20 ± 0.1°C, 상대습도 60 ± 0.1%, 이산화탄소 938.2 ± 5.8μmol·mol<SUP>-1</SUP>, 광도 PPFD 250.6 ± 0.6μmol·m<SUP>-2</SUP>·s<SUP>-1</SUP>, 광주기 16시간으로 설정된 인공광 이용형 식물공장에서 5주 재배되었다. 광질 처리조건은 대조구로 식물재배용 3가지 백색 LED(CL 1, CL 2, CL 3), 백색 LED 그룹[Warm W(WW), Neutral W(NW), Neutral W + Red(NWR), Cool W(CW)], 원적색광이 추가된 백색 LED 그룹(WWFR, NWFR, NWRFR, CWFR)이 사용되었다. 백색 LED에 원적색광의 추가는 지상부 생육특성 항목(생체중, 엽장, 엽면적)을 증대시켰고 WWFR의 지상부 생장이 유의적으로 가장 높았다. 반면에 원적색광의 보광은 엽록소 함량을 감소시켰고 플라보노이드 지수에는 영향을 주지 않았다. 재배기간 동안의 총 소비전력은 CL 1에서 가장 낮았고 WWFR의 총 소비전력이 가장 높았다. WW와 WWFR의 광 이용효율이 높았으며 백색 LED에 원적색광의 추가는 광 이용효율을 3–15% 증대시켰다. 따라서, 백색 LED에 원적색광의 보광은 작물의 생산성과 광 이용효율을 향상시켜 작물의 생산비용을 감소시킬 수 있었다. The electric energy consumption of artificial lighting sources is a major factor to increase the production cost in plant factories with artificial lighting (PFALs). Therefore, improving the light use efficiency of lighting sources is an important task in securing the economic feasibility of PFALs. The purpose of this study was to compare the productivity of Crepidiastrum denticulatum (Houtt.) Pak & Kawano and the light use efficiency of various white LEDs with or without far-red light. Three-week-old seedlings were transplanted in wick culture systems and grown under 20.9 ± 0.1°C air temperature, 60.8 ± 0.1% relative humidity, 938.2 ± 5.8 μmol·mol<SUP>-1</SUP> CO₂ concentration, 250.6 ± 0.6 μmol·m<SUP>-2</SUP>·s<SUP>-1</SUP> PPFD, and 16 h light period in PFAL. Light treatments used were as followed; 3 types of commercial white LEDs (CL 1, CL 2, CL 3) as controls, white LEDs [Warm W (WW), Neutral W (NW), Neutral W+Red LEDs (NWR), Cool W(CW)], and white LEDs with far-red LEDs (WWFR, NWFR, NWRFR, CWFR). Supplemental far-red radiation to white LEDs increased the shoot growth characteristics (shoot fresh weight, leaf length, and leaf area), and the shoot growth of plants in WWFR was the highest. On the other hand, supplemental far-red light decreased the chlorophyll index and did not affect the flavonoid index. Total electric power consumption was the lowest in CL 1 and was the highest in WWFR. The light use efficiency of WW and WWFR was high, and supplemental far-red light to white LEDs increased the light use efficiency by 3–15%. Therefore, supplemental far-red radiation to various white LEDs could reduce the plant production cost by improving the productivity and the light use efficiency of crops in PFALs.

ossible Relationship between AMPK and Apoptosis in HT-29 Colon Cancer Cells Treated with CLA

박송이,Yun-Kyoung Lee,Won Sup Lee,Ock Jin Park,Young-Min Kim 대한암예방학회 2008 Journal of cancer prevention Vol.13 No.4

Conjugated linoleic acid (CLA), an essential fatty acid linoleic acids (LA), has been shown to suppress cancer cell proliferation and induce apoptosis in several cancer cells. AMP-activated protein kinase (AMPK), a highly conserved protein in eukaryotes, function as an intrinsic regulator of mammalian cell cycle and primarily as a sensor of cellular energy level. In this study, we have examined the effect of CLA on apoptosis and the regulatory effect of AMPK and cyclooxygenase-2 (COX-2) pathway in HT-29 colon cancer. Treatment of CLA (20∼320μM) for 24 h inhibited proliferation of cancer cells and increased chromatin condensation levels represented apoptotic death. These apoptotic effects seemed to be related to AMPK-COX-2 pathways. CLA strongly activated AMPK at the concentration of 40μM and above and in the same condition, COX-2 expression was decreased in cancer cells. ROS is a possible upstream signal of AMPK. CLA generated reactive oxygen species (ROS) at the concentration of 40μM and 160μM in HT-29 colon cancer cells. These observations suggest that AMPK has an important role in cancer cell apoptosis induced by CLA and further AMPK-COX-2 pathway may be a possible target in cancer control.