Helicobacter pylori성 위염에 대한 Mastic Gum의 효과

노임환 ( Im Hwan Roe ),남승우 ( Seung Woo Nam ),명나혜 ( Na Hye Myung ),김정택 ( Jung Taik Kim ),신지현 ( Ji Hyun Shin ) 대한소화기학회 2003 대한소화기학회지 Vol.41 No.4

Background/Aims: Mastic, a natural resin obtained from the Pistacia lentiscus tree, has been reported to have an antimicrobial activity against Helicobacter pylori (H. pylori). We investigated the effects of mastic containing chewing gum on the subjects with H. pylori-infected gastritis. Methods: A double blind clinical trial was carried out on forty-eight volunteers with H. pylori infected gastritis. They were divided into age and sex matched two groups and then treated with the chewing gum containing mastic (1 mg/piece) and placebo, 3 times for 15 minutes a day before meals for 90 days. The 13C-urea breath test and upper gastrointestinal endoscopy with biopsy for rapid urease test and histologic examination were performed at 0, 30, and 90 days after treatment in all subjects including 15 H. pylori-negative controls. Results: All subjects were well compliant. The treatment with mastic gum led to significant decrease in △13C(‰) values. The △13C(‰) value measured at 90 days was significantly lower than those at 0 and 30 days (p=0.033, p=0.034, respectively). Moreover, significant improvement was observed in active gastric inflammation at 90 days compared to before the treatment (p=0.004). Placebo group did not show any changes in △13C(‰) values and active inflammatory scores according to treatment duration. Conclusions: Mastic gum has a beneficial effect on H. pylori-infected gastritis, and can be considered as one of novel treatments. (Korean J Gastroenterol 2003;41:277-283)

관련성 위장질환 치료를 위한 계란항체 ( Immunoglobulin Yolk ) 의 기대 효과

노임환(Im Hwan Roe),남승우(Seung Woo Nam),양미라(Mie Rha Yang),명나혜(Na Hye Myung),김정택(Jung Taik Kim),신지현(Ji Hyun Shin) 대한소화기학회 2002 대한소화기학회지 Vol.39 No.4

Background/Aims: The importance of eggs as a source of specific antibodies (IgY) is well recognized. We investigated the efficacy of specific anti-Helicobacter pylori (H. pylori) IgY antibody against H. pylori infection. Methods: Specific anti-H. pylori IgY was obtained from the hens which were immunized with whole cell lysate (200 킽/mL) of H. pylori. Specific IgY was recognized by SDS-PAGE and the activity was determined by ELISA. After administration of specific IgY, the growth changes of H. pylori, the response of H. pylori-infected KATO III and AGS cell, and in vivo effect of IgY on H. pylori-infected gerbil model were determined. Results: Specific IgY inhibited the growth of H. pylori by 90% within 6 hours in contrast to no inhibitory effect on the growth of E. coli or L. salivarius. After treating with 10 mg/mL of specific IgY, H. pylori-infected (107 CFU/mL) KATO III and AGS cell did not proliferate. Bacterial adhesion was definitely decreased in H. pylori-infected AGS cell treated with 10 mg/mL of IgY. Oral administration of 10 mg/mL of IgY daily for 30 days remarkably inhibited gastric inflammation in H. pylori-infected gerbil, whereas 1 mg/mL of IgY did not reduce gastric inflammation. Conclusions: Specific anti-H. pylori IgY might provide promising effects on the H. pylori-associated gastric diseases. (Korean J Gastroenterol 2002;39:260-268)

대한내과학회 연구비 결과보고 : Helicobacter pylori의 간균형에서 구균형으로의 형태 변화에 따른 전자 현미경적 관찰 및 항원발현의 변화에 관한 연구

노임환 ( Im Hwan Roe ),신지현 ( Ji Hyun Shin ),이학성 ( Hak Sung Lee ),이종화 ( Jong Hwa Lee ) 대한내과학회 1999 대한내과학회 춘계학술대회 Vol.1999 No.2

Biotin 표지 HBV DNA 를 이용한 Dot Hybridization 에 관한 연구

노임환(Im Hwan Roe),이동후(Jung Hye Roe),노정혜(Dong Hoo Lee) 대한소화기학회 1988 대한소화기학회지 Vol.20 No.3

N/A A dot blot hybridization technique utilizing a biotin-labelled recombinant DNA probe was used to examine hepatitis B virus (HBV) DNA in serum. The lowest amount of HBV DNA in serum detectable by the color development of an avidin-biotin alkaline phosphatase complex was 40 picogram per 50 microliter. Validity of this method was confirmed by autoradiography using 32P-labelled and 3H-labelled HBV DNA probes. HBV DNA was found in 100% (34/34) of the HBsAg-positive and in 73.5% (25/34) of the HBsAg-negative subjects. In contrast, all nine cases showing negativity in HBV DNA were also HBsAg-negative. Correlation of Hbe antigen/antibody with HBV DNA was investigated in 19 sera of which HBsAg was negative but anti-HBc positive. Of 13 sera with anti-Hbe 10 (76.9%) cases revealed HBV DNA positivity, while four (66.7%) of six sera without anti-HBe were positive in HBV DNA. In conclusion, serum dot hybridization assay utilizing a bir>tinylated probe proved useful in the detection of a free form of HBV DNA regardless of the presence of HBsAg and irrespective of HBeAg-anti-HBe status. Also an anti-HBs does not always correspond to the clearance of HBV. And this indicates, in turn, that the potential infectivity of certain sera previously considered to be safe should be kept in mind. Moreover, it is emphasized that practical advantages in speed, reproducibility, and safety have made this alternative even more attractive than autoradiography using radioisotope- labelled probes.

위십이지장질환에서 Helicobacter Pylori 의 DNA Variation 에 관한 연구

노임환(Im Hwan Roe),김창인(Chang In Kim),하동렬(Dong Ryul Ha),진영주(Young Joo Jin),송일한(Il Han Song),임창영(Chang Young Lim),김정원(Jung Won Kim),김정택(Jung Taik Kim),이종화(Jong Hwa Kim),염정선(Jung Sun Yeom) 대한내과학회 1997 대한내과학회지 Vol.53 No.4

N/A Background: The evidence for H. pylori as a gastrointestnal pathogen is now very strong, if not overwhelming. Among the pathogenic factors of H. pylori, flagella and urease are considered to be major factors causing the gastrododenal disease. We observed the gene diversity of H. pylori using the PCR-amplified 1.4Kb fla A gene and 0.9Kb ure B gene and examined the relationship between the gene pattern and the gastroduodenal disease. Method: Fifty-one cases of isolated strains were cultured at the Helicobacter-selective blood agar plates. To compare the gene diversity among the isolates of gastroduodenal disease genotypes was analyzed by PCR-based RFLP. 1.4Kb fla A gene and 0.9Kb ure B genes from isolates were amplified by PCR and digested with Hae 3 restriction enzymes to observe the restriction fragment length polymophysm. Protein patterns were also compared to examine the antigenic variations. Total cell proteins, and octyl-glucose extracts from isolates were analyzed by SDS-PAGE gel electrophoresis. Results: 41 cases (80.4%) of H. pylori were isolated in the 51 cases of gastroduodenal diseases. We could classify theses isolates 3 types of PCR-RFLP in the fla A gene, 900+500bp, 500+500+400bp, 600+800bp, and 9 types in the ure B gene. PCR-RFLP in the fla A gene and ure B gene of the isolates was different from the standard strain of Australia and the genetic diversity was not related to the types of the gastroduodenal disease. We demonstrated variations in the protein pattern and antigenic profiles among the isolates by SDS-PAGE analysis. These data also did not show any relationship between protein pattern and types of gastroduodenal diseases. Conclusion: Tese studies showed many different gene diversity in the flagella and urease gene without any relationship with the types of gastoduodenal disease. And variable protein pattern were noted among the strains of H. pylori. Further studies to demonstrate the pathgenecity of H. pylori should be continued even if there was no relationship between the genomic diversity of the flagella or urease and the types of gastroduodenal disease.

만성특발성 변비 및 당뇨병성 변비 환자에 대한 대장통과시간의 비교 연구

노임환(Im Hwan Roe),정현채(Hyun Chae Jung),김성연(Sung Yun Kim),이효석(Hyo Suk Lee),윤용범(Yong Bum Yoon),송인성(In Sung Song),최규완(Kyoo Wan Choi),김정룡(Chung Yong Kim),최병인(Byun In Choi) 대한내과학회 1990 대한내과학회지 Vol.39 No.5

N/A Measurement of colonic transit time with radiopaque markers is a simple and useful modality in evaluating canstipation. We calculated the segmental colonic transit time in healthy controls (n=34), chronic idiopathic constipated patients, and constipated patients with diabetic autonomic neuropathy (n=14), Total colonic transit time was 24.1±8.1 hrs for the healthy controls, 21.1±8.8 hrs for men, and 25.8±5.3 hrs in women. Also, the segmental transit time was 3.9±1.7 hrs for the right colon, 11.8±5.2 hrs for the left colon, and 8.5±5.5 hrs for the rectosigmoid. The mean colonic transit time did not differ significantly by age or sex. The proportion of the transit time in the right colon, left colon, and the rectosigmoid were 17.4%, 51.6%, and 32.4%, respectively. The chronic idiopathic constipated patients were 14 females, whose total and segmental transit times were delayed markedly; 74.2±40.9, 18.7±14.4, 33.4±51.6, and 22.1±15.7 hrs for the total colon, right colon, left colon, and rectosigmaid, respectively (p<0.001). These proportions showed no significant difference compared with the healthy controls: 24.0%, 50,5%, and 24.7% for the right colon, left colon, and rectosigmoid, respectively, In 14 cases with constipated diabetic autonomic neuropathy, the colonic transit time was also delayed: 55.1±16.8, 16.2±7.0, 17.0±8.8, and 22.0±11.7 hrs for the total colon, right colon, left colon, and rectosigmoid, respectively. These proportions showed a significant delay of transit time in the rectosigmoid: 29.9%, 31.0%, and 41.2% in the right colon, left colon, and rectosigmoid, respectively, p<0.05, Also, in the non-constipated patients with diabetic autonomic neuropathy, the proportion of colonic transit in the rectosigmoid was as high as 46.6%, even though the transit time was not delayed compared with the healthy controls. It was remarkable that transit in the rectosigmoid was prolonged in the diabetic autonomic neuropathy.

호두까기 식도증과 칼슘 차단제의 투여효과

노임환(Im Hwan Roe),황흥곤(Heung Kon Hwang) 대한내과학회 1995 대한내과학회지 Vol.49 No.6

N/A Objectives : Recently, the nutcracker esophagus has been considered as a major motility defect causing the non-cardiac chest pain, and the pain has usually attributed to the exaggerated contractions of the distal esophageal body. We investigated the effects of the calcium channel blocker on the esophageal contractions and LES pressure. Methods: Thirty four patients with the nutcraker esophagus were administered Diltiazem (180- 270mg/D, N=20) or Nifedipine(30-40mg/day, N=14) for 3 months. Patients underwent manometric testing with the 4 lumens polyvinyl cathter of noninfusion system(SANDHILL TDS 4000) before and after treating with Diltiazem or Nifedipine. Results: Significant pain relief was observed in 28 of 34 cases; 15 of 20 Diltiazem treated cases and 13 of 14 Nifedipine treated ones. Side effects such as headache, dizziness, edema developed in about 30Yo of the Nifedipine treated group, whereas there was no side effects in the Diltiazem treated group. Treatment with Diltiazem or Nifedipine decreased mean distal amplitude by 28.9% and 18.4%, respectively, and mean duration by 27.1% and 16.5%, respectively, Nifedipine reduced LES pressure significantly(p<0.05), whereas Diltiazem reduced distal amplitude end duration much more than Nifedipine did. Conclusion: These studies suggest that calcium channel blockers are effective in treating patients with the nutcracker esophagus, even though the cause of the esophageal chest pain is not consistently related with the distal esophageal contractions of high amplitude, long duration and multiplicity. And also Diltiazem is considered to be better to the patients with the nutcraker esophagus due to the minimal side effects. Key Words: Nutcraker esophagus , Non cardiac chest pain , Ca ehannel blocker

암모니아로 인한 위점막 손상의 연구 - Helicobacter pylori의 위장질환 발생기전과 관련하여

노임환(Im Hwan Roe),양미라(Mi Ra Yang),김정택(Jung Taik Kim),조정희(Jung Hee Joe),이종화(Jong Hwa Lee) 대한소화기학회 1997 대한소화기학회지 Vol.29 No.4

N/A Background/Aims: After the identification of Helicobacter pylori as a major cause of gastro- duodenal diseases, special attention was paid to elucidating the mechanism of tissue injury and mucosal inflammation. Among the many exotoxins of H.pylori, ammonia was considered to be a resistant factor against the acidic condition, and to be a pathogenic factor causing mucosal injury. We studied the pathogenesis of the ammonia induced gastric mucosal injury. Methods: We perforrned biochemical and pathological experiments in male Sprague Dawley rats(N=51, 200+ 30gm). The rats were fasted and were killed at 2, 10, and 30 minutes following administration of lml of ammonia(A2, A10, A30, N=6 respectively), or 75% ethanol(E2, E10, E30, N=5, respectively). Additional groups of rats sacrificed on the 8th day following daily administration of 1ml of 1% ammonia(AC, N=6) or 75% ethanol (EC, N=6). After death, some portion of the stomach was rapidly homogenized and processed for the determination of sulphydryls, glutathione(GSH), and malondialdehyde(MDA). Additionally some portion of the stomach was examined pathogically. Results: GSH(p mol/gm tissue) in the rat's gastric mucosa changed from 2.4+0.4 in controls to 1.0+0.3, 0.5+0.1, 0.5+O.l, 0.6+0.1, or 1.4+0.2, 0.4+0.1, 0.5+0.1, and 0.6+0.2 in A2, A10, A30, AC, or E2, E10, E30, and EC, respectively. The level of GSH was significantly decreased(P<0.01) in A2 compared to E2. Also MDA(nmol/gm tissue) in the rats gastric mucosa changed from 26.3+4.4 in controls to 75.7+25.8, 59.9+13.1, 47.6+11.1, 69.2+ 25.3, or 46.3+18.1, 63.6+23.9, 46.8+20.0, and 71.9+20.9 in A2, A10, A30, AC, or E2, E10, E30, and EC, respectively. MDA was significantly increased(P<0.01) in A2 compared to E2. The gastric mucosa in A2 showed more severe hemorrhage and ischemia than E2(50Fo, 30% of the stomach in A2, VS 30%, 0% of the stotnach in E2,) Conclusions: The depletion of the mucosal sulphydryls, GSH, the release of free radicals, and early vascular injury are considered to play an important role in the pathogenesis of ammonia and H.pylori induced gastric injury, and ammonia was found to be more damaging in the rats stomach than ethanol in the early stage of injury. (Korean J Gastroenterol 1997; 29:442-448)

생장 억제 물질로서의 마늘의 효과 - In Vitro , In Vivo 연구

노임환(Im Hwan Roe),남승우(Seung Woo Nam),명나혜(Na Hye Myung),김정택(Jung Taik Kim),신지현(Ji Hyun Shin) 대한소화기학회 2002 대한소화기학회지 Vol.40 No.3

N/A

생체 내 , 외에서 Helicobacter pylori 생장 억제 물질의 탐색 및 효능의 검증

노임환(Im Hwan Roe),남승우(Seung Woo Nam),명나혜(Na Hye Myung),김정택(Jung Taik Kim),신지현(Ji Hyun Shin) 대한소화기학회 2002 대한소화기학회지 Vol.40 No.3

N/A