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Bacillus licheniformis CN - 115 균주를 이용한 청국장 제조 과정에 있어서 단백질 및 아미노산의 변화
석영란(Yeong Ran Seok),김영활(Yung Hawl Kim),김성(Sung Kim),우희섭(Hi Seob Woo),김태완(Tae Wan Kim),이선호(Son Ho Lee),최청(Cheong Choi) 한국응용생명화학회 1994 Applied Biological Chemistry (Appl Biol Chem) Vol.37 No.2
Chungkook-Jang was produced by fermenting Bacillus licheniformis CN-115. The changes of chemical composition, enzyme activity, and amino acids during the fermentation were investigated. The proximate composition was shown irregular fluctuation phenomenon during the fermentation, but only the moisture tended some reducing during the fermentation just after steaming. The content of amino nitrogen was increased radically after the 36 hours of fermentation and became the highest level at 18.072 ㎎/g at the 60 hours of it. In accordance with the fermentation of Chungkook-Jang, pH got to the 8.39 at 60 hours with increasing, protease activity was increased according to the fermentation and acid and neutral protease activity was reduced after being reached at the highest activity at 48 hours. The most suitable pH was 6.5 and temperature was 35℃ for dissolution-activated of protein in the process of fermentation of Chungkook-Jang. The content of water soluble protein and the content of salt soluble protein were increased at continuously according to the fermentation time of Chungkook-Jang the largest quantity. The molecular weight of water soluble protein of Chungkook-Jang fermented for 48 hours was about 19,000. The amino acids of water soluble protein just after steaming were totally 16 kinds and proline was amino acid and them was in series by glutamic acid and serine in that ordered. The amino acids salt soluble protein, just after steaming were totally 16 kinds and was the largest quantity phenylalanine, glutamic acid and aspartic acid and aspartic acid in that order.
정 관석,나 영왕,심 상인,김 석현 한국작물학회 2014 한국작물학회지 Vol.59 No.3
Field emergence of Azuki bean is poor due to hard seed coat as compared to other legumes. In this study, an attempt was made to develop prediction method with regression analysis based on various seed vigor tests in laboratory for field emergence of azuki bean. Azuki bean seeds artificially aged to provide various levels of seed quality were evaluated by the standard germination test (SGT), cold germination test (CT), cool germination test (CGT), complex stressing vigor test (CSVT), tetrazolium(TZ) vigor test and electroconductivity test. The SGT was suitable for predicting the field emergence in the unaged high vigor seeds. The abnormal seedling percentage and shoot length in the CGT were highly correlated with field emergence of moderate vigor seeds artificially aged for 2 days. Electroconductivity, seed viability in the CSVT, and vigor and predicted germinability in the tetrazolium vigor test were also useful for predicting field emergence. Percent of ungerminated seed in the CSVT was correlated with field emergence in the low vigor seeds artificially aged for 4 days. In a stepwise multiple regression analysis, seed viability in the SGT, normal seedling percentage and dry matter weight in the CGT accounted for 86.9% of the predicted value of field emergence in azuki bean.
나 영왕,최 유미,백 형진,이 석영,강 정훈,김 석현 한국작물학회 2014 Korean journal of crop science Vol.59 No.3
The purpose of this study was to know the seed longevity of rice (Oryza sativa L.) germplasm for effective viability monitoring. The longevity was determined via germination tests of 3,066 accessions of rice germplasm from the National Agrobiodiversity Center, Rural Development Administration, Korea. The rice germplasm accessions have been conserved at a mid-term storage (4°Csealed metal can on either sides for 25~26.5 years. The final germination percentages of 3,066 rice germplasm accessions of 6.5±1.0% seed moisture content with 94% initial germination stored at 4°C for 26.5 years declined to 47% while at -18°C for 25 years maintained high germinability as 93%. Germination time courses, which represent the average performance of rice ecotypes stored at 4°C and 30% RH, were fitted regression equation, to calculate the time at which germination characteristically declined to 50% (P50). These P50 values of Indica, Japonica, Javanica and Tongil type in rice were 39.9, 22.9, 25.4 and 31.8 years, respectively. The rice germplasm stored at 4°C could be clustered in 4 groups using quartile of final germination after 26.5 years storage. The seed longevity (P50) of each group was estimated by regression equation of changed germination percentages according to storage periods. The P50 values of group Ⅰ, group Ⅱ, group Ⅲ and group Ⅳ were 21.1, 23.6, 30.0 and 75.7 years.
나 영왕,백 형진,최 유미,이 석영,이 정로,정 종욱,박 용진,김 석현 한국작물학회 2014 한국작물학회지 Vol.59 No.3
The purpose of this study was to develop the cost-effective and efficiency seed longevity prediction method of rice (Oryza sativa L.) germplasm for viability monitoring. To find an optimum predicting method for rice seed longevity at genebank, an accelerated ageing (AA) test, a controlled deterioration (CD) test and a dry-heat treatment (DHT) were conducted to the four groups of rice germplasm based on ecotype, such as Indica, Japonica, Javanica and Tongil type. Among the three artificial aging treatments, the dry-heat treatment of 36 hours at 90°C is suggested as a routine predictive test method of rice germplasm longevity at a genebank. The distribution of germination rate on 3,066 accessions which conserved 26.5 years at 4°C showed similar trend with the result of distribution by dry-heat treatment at 90°C on 36 hours using 106 accessions of rice selected samples which composed four ecotype groups. The results show that the dry-heat treatment affect not only predicting the rice seed longevity but also determining effective interval for monitoring germination of rice germplasm in genebanks.
김오종,유선경,노희권,기창돈,최민우,석효정,윤동환,박병운,지철규 한국항공우주학회 2017 International Journal of Aeronautical and Space Sc Vol.18 No.3
Among various applications of the High Altitude Long Endurance (HALE) Unmanned Aerial Vehicle (UAV), this paper has a focus on the Global Positioning System (GPS) utilizing pseudolite and its improved performance, particularly during the multi-purpose missions. In a multi-purpose mission, the HALE UAV follows a specified flight trajectory for both navigation applications and missions. Some of the representative HALE missions are remote exploration, surveillance, reconnaissance, and communication relay. During these operations, the HALE UAV can also be an additional positioning signal source as it broadcast signals using pseudolite. The pseudolite signal can improve the availability, accuracy, and reliability of the GPS particularly in areas with poor signal reception, such as shadowed regions between tall buildings. The improvement in performance of navigation is validated through simulations of multi-purpose missions of the solar-powered HALE UAV in an urban canyon. The simulation includes UAV trajectory generation at stratosphere and uses actual geographical building data. The results indicate that the pseudolite-equipped HALE UAV has the potential to enhance the performance of the satellite navigation system in navigationally degraded regions even during multi-purpose operations.