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시금치에서 분리한 광계 2 복합체의 불활성화와 재활성화에 대한 연구
김현식(Hyun Sik Chun),박인호(In Ho Park),이혜주(Hae Joo Lee),선우양일(Yang Il Sun Woo),이진범(Chin Bum Lee) 한국식물학회 1990 Journal of Plant Biology Vol.33 No.4
Inactivation and reactivation of photosynthetic oxygen evolving complex were studied with isolated spinach (Spinacia oleraceda. L.) photosystem Ⅱ particles by the activity of oxygen evolution and chlorophyll fluorescence. When the particles were treated with Tris and urea, the oxygen evolution was inactivated and three polypeptides having molecular weights of 33 kDa, 24 kDa and 18 kDa were simultaneously released. But in NaCl-treated particles, two polypeptides of 24 kDa and 18 kDa were removed from PS Ⅱ particles. The oxygen evolution activities of Tris and urea-treated particles were not restored by adding cation ions (Mg^2+, Mn^2+ and Ca^2+), but the NaCl-treated particles were restored by exogenously added Ca^2+. The removal of these extrinsic polypeptides, especially 33 kDa, markedly showed the decrease of the variable fluorescene (Fv). These results are likely to be due to dissipate thermal energy by antenna of photosystem Ⅱ complexes.
전종균,김강부,김현식 선문대학교 ·중소기업기술지원연구소 1999 선문공대 연구/기술 논문집 Vol.4 No.1
본 연구에서는 공작기계의 이상상태 감시 및 진단 기법을 기술하였다. 특히, 주요 진단부를 스핀들과 이송축으로 선정하여 각 신호를 가속도, 회전오차, 이송속도, 부하, 온도 등으로 종류별로 측정하였다. 또한, 시편을 1mm와 4mrn로 절삭하여 측정한 신호를 비교하였고 공작기계 감시 및 진단 시스템을 구축하기 위한 기본적인 장치구성을 정립하였다. Monitoring and diagnosis technique of abnormal states in machine tool was studied. Especially, a spindle and feed-axis were selected as a main diagnosis part, and acceleration, rotational gap, feed speed, load capacity, and temperature were measured. Specimens were examined by cutting in 1mm and 4mm to compare the signals with each other. Basic experimental apparatus was established for monitoring and diagnosis system of machine tool.