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흰쥐 간의 Aryl hydrocarbon hydroxylase 에 대한 인삼 추출물의 선택적 효과
선우양일,박기현 ( Yang Il Sun Woo,Ki Hyun Park ) 생화학분자생물학회 1984 BMB Reports Vol.17 No.4
Many carcinogens of polycyclic aromatic hydrocarbons including BP are biotransformed by aryl hydrocarbon hydroxylase (AHHase, EC 1. 14. 14. 1) found in human and animal tissues into reactive intermediates such as epoxides. AHHase is a component of cytochrome P-450 (P-450) isozymes, and the P-450-containing enzyme system of liver microsome is remarkably versatile in the types of chemical reactions it catalyzes and in its choice of substrates. Accordingly, to evaluate the effects of ginseng extracts on AHHase activity, we treated intraperitoneally Sprague-Dawley rats (male, 160-180 g) with phenobarbital (Pb) or 3-methylcholanthrene (3-MC) as inducers of P-450. When the methanol or pet. ether extract of ginseng was added in hepatic microsome separated from rat untreated with chemical, AHHase activities in the both cases were sharply inhibited. Also, the changes of AHHase activities from microsome treated Pb were similar observations such as rats untreated. The AHHase activities from microsome treated with 3-MC as a potent inducer of P-450 were exhibited the opposite results by methanol extract as compared to that of untreated or Pb-treated rats, whereas pet. ether extract had no any effects for AHHase activities. Water extract did not have any effect such as results evaluated from untreated or Pb-treated rats. These results demonstrate that the activating and inhibiting effects of pet. ether or methanol extract depend on the isozyme type of P-450 induced with Pb or 3-MC.
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시금치에서 분리한 광계 2 복합체의 불활성화와 재활성화에 대한 연구
김현식(Hyun Sik Chun),박인호(In Ho Park),이혜주(Hae Joo Lee),선우양일(Yang Il Sun Woo),이진범(Chin Bum Lee) 한국식물학회 1990 Journal of Plant Biology Vol.33 No.4
Inactivation and reactivation of photosynthetic oxygen evolving complex were studied with isolated spinach (Spinacia oleraceda. L.) photosystem Ⅱ particles by the activity of oxygen evolution and chlorophyll fluorescence. When the particles were treated with Tris and urea, the oxygen evolution was inactivated and three polypeptides having molecular weights of 33 kDa, 24 kDa and 18 kDa were simultaneously released. But in NaCl-treated particles, two polypeptides of 24 kDa and 18 kDa were removed from PS Ⅱ particles. The oxygen evolution activities of Tris and urea-treated particles were not restored by adding cation ions (Mg^2+, Mn^2+ and Ca^2+), but the NaCl-treated particles were restored by exogenously added Ca^2+. The removal of these extrinsic polypeptides, especially 33 kDa, markedly showed the decrease of the variable fluorescene (Fv). These results are likely to be due to dissipate thermal energy by antenna of photosystem Ⅱ complexes.
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옥수수 분리 엽록체에서의 엽록소 형광의 소멸과 광계 2 의 Quantum Yield
이진범,박인호,이혜주,선우 양일 ( Chin Bum Lee,In Ho Park,Hae Joo Lee,Yang Il Sun Woo ) 생화학분자생물학회 1991 BMB Reports Vol.24 No.6
The relationship between quantum yield of photosystem II and quenching component of chlorophyll fluorecene has been investigated through a series of experiments on isolated maize mesophyll chloroplasts. The thylakoid membranes of higher plants possess several mechanisms that control both the distribution and the rate of dissipation of absorbed light. These mechanisms allow regulation by external and internal factors. By using DCMU addition techniques, there is an advantage to be able to distinguish energy-dependent quenching (qE) from other components of non-photochemical quenching (qNP). At low light intensity, photochemical quenching (qQ) is major component of chlorophyll fluorescence quenching, while at high light intensity, qNP is major one, The relationship between qQ ans quantum yield of oxygen evolution (Φ_s) with incident light intensity was showed. The nonlinear relation is indicative of the decline in the intrinsic yield of photosystem II (Φ_p) at high light intensity. Φ_p declined as the light intensity increased, which was inversely proportional to qE. The oxygen evolution was increased gradually as the temperature increases, but over 45℃, decreased rapidly. qE, qR (the remaining quenching) and qNP were continuously decreased, while qQ was rapidly increased over 30℃. The changes of (F_v)_m,/F_m on temperature may be presumably able to suggest that they are affected by the phophorylation of LHCII. And interestingly, the changes of F_v)_m,/F_m were very similiar with those of Φ_p.
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