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담수어의 (淡水魚) 지리적 집단간의 수용성 근단백 분획에 대하여 (1)
김지식,전상린 ( Ji Sik Kim,Sang Rin Jeon ) 한국하천호수학회 1981 생태와 환경 Vol.14 No.1·2
To study the difference of aqueous soluble muscle protein pattern of fresh-water fishes in geographical populations the method of disc-electrophoresis using polyacrylamide was adapted. 1) The number of bands of aqueous soluble muscle protein were 10 in Zacco platypus, 7 in Zacco temmincki, 6 in Pungtungia herzi, 7 in Pseudopungtungia tenuiscorpus, 5 in Rhodus ocellatus, 8 in Coreoleuciscus splendidus, 9 in Microphysogobio longidorsalis. 2) The number of bands, degree of stain, mobility and rate of contents were different in interspecies. 3) No electrophoretic pattern was observed different among the four locality.
SrAl<sub>2</sub>O<sub>4</sub>(Eu,Dy,Nd) 압광체를 이용한 균열첨단에서의 응력장 가시화 연구
김지식,손기선 한국소성가공학회 2003 소성가공 : 한국소성가공학회지 Vol.12 No.5
The present investigation aims at visualizing the crack tip stress field using a mechanoluminescence material. The well known compound $SrAl_2O_4$:$Eu^{2+}$ was adopted as a mechanolurninescence material. Two more trivalent rare-earth elements such as Dy and Nd were taken into consideration as codopants to provide the appropriate trap levels. Samples of a variety of compositions were prepared by varing $Eu^{2+}$, $Dy^{3+}$, and $Nd^{3+}$ doping contents, for which the combinatorial chemistry method was used. In order to search for the optimum composition for the highest mechanoluminescence, the luminescence induced by a compressive device including a CCD camera. In parallel, a compact tension specimen was prepared by mixing the luminescence powders of optimum composition and epoxy resin. Crack initiation from the mechanically machined sharp note tip and its growth during loading were found to be associated with the extent of light emission from $SrAl_2O_4$.
초파리 자연집단의 P 전이인자에 대한 계통형 분포와 기능에 관한 연구
김지식,권도형추종길 한국통합생물학회 1995 동물학회지 Vol.38 No.2
두 지역에서 채집한 초파리 자연집단에 대하여 난소발생이상 실험에 의한 P 인자 활성과 세포질형을 분석하여 P 전이인자의 계통형을 조사하였다 전체 238 isofemale line을 조사한 결과 strong P와 true M은 존재하지 않았고, 0(weak P)와 M'(pseudo M) strain이 전체의 98.74%를 차지하여 가장 우세하게 분포하고 있었다. P$\pi$25.1 probe를 이용한 in situ hybridization을 행하여 P 전이인자의 copy수를 조사한 결과 평균 42.12개로 나타났으며, 0와 M'의 계통형 간에 유의적인 차이는 없었다 그러나 염색체 firm당 COPy수는 X염색체가 상염색체의 좌 우 각 arm보다 다소 높게 분포하고 있었고. 염색체상 P 전이인자의 삽입부위에 대한 특이적 좌위는 존재하지 않았다 P 전이인자의 분자구조에 대한 변이형을 조사하기 위하여 southern blot hybridization을 행한 결과 2.9kb의 완전한 크기의 분자를 포함하여 여러종류의 단편들이 확인되었다 조사한 모든 isofemale line에서 KP(1. 15kb)인자를 포함하고 있었으며 이들 KP인자가 P-M System의 난소발생이상을 표현하는데 있어 억제적 작용을 하는 것으로 판단되었다.
SrAl₂O₄: (Eu, Dy, Nd) 압광체를 이용한 균열 전파 및 브리징 응력의 가시화 연구
김지식,권용남,손기선 대한금속재료학회 2004 대한금속·재료학회지 Vol.42 No.8
The goal of the present investigation was to visualize the propagating crack in a mechano-luminescence (ML) material to enable the measurement of instantaneous R-curves and directly observe the bridging (shielding) stress in a fast-propagating crack system. The well-known ML compound, SrAl₂O₄: Eu^(2+), was used as a model test material. Two additional trivalent rare earth elements, Dy and Nd, were introduced as co-dopants to improve the luminescent efficiency. The initiation and growth of a crack from the mechanically machined sharp notch tip in a disc shaped compact tension (CT) specimen at a relatively high loading rate were found to be associated with the extent of light emission around the crack. An in-situ measurement of crack length and applied load for 0.3 sec yielded an instantaneous R-curve at the conventional crack propagation speed.
김지식,Kevin Kibble,Mark Stanford,손기선,권용남 대한금속·재료학회 2009 METALS AND MATERIALS International Vol.15 No.4
The propagations of macro-scale cracks were visualized in Si3N4 and SiC ceramics using a mechano-luminescence (ML) of SrAl2O4:Eu,Dy. The bridging zones behind the crack tip were also clearly detected in the crack path within realistic time frames. The ML made it possible to detect cracks propagating within a speed range of 200 m/s to 250 m/s, thereby realizing so-called quasi-dynamic R-curves. The magnitudes and shapes of the bridging stress distributions were found to change with the advancing cracks, continuing to change as the applied load changes, even after the cessation of crack propagation. The effective toughening then commenced and the applied stress intensity factors increased dramatically up to ~120 MPa √m in Si3N4 and 150 MPa √m in SiC. The KTip values expected on the basis of the instantaneous bridging stress distributions obtained from ML observations deviated greatly from those measured using the conventional crack tip lengths; rather, the values support the results obtained by using bridging tips in quasi-dynamic crack propagations.
김지식 群山大學校基礎科學硏究所 1999 基礎科學硏究 Vol.14 No.-
Leghorn계인 Shaver star 288(Domestic fowl)의 부란 18일의 배와 부화 후 3일된 암 병아리, 110일, 134일, 148일된 암탉, 173일 된 산란이 정지된 암탉, 그리고 산란전 시기에 있는 139일된 암탉에 estradiol benzoate로 처리한 것의 혈청단백질을 filter paper를 이용한 zone-electrophoresis에 의하여 영동상을 비교하였다. 1. 18일된 배에서 4개의 영동대를 관찰할 수 있었고 albumin 앞에 pre-a.bumin을 볼 수 있었다. 2. 3일, 134일, 148일된 암탉에서는 5개의 영동대가 나타났는데 이는 성계 에서는 배의 혈청에 있던 pre-albumin이 소실된 반면 α₂, γ-globulins이 나타난 것이다. 3. 173일된 산란계는 6개의 영동대가 나타났으며 X영동대가 나타났다. 4. 520일된 산란이 정지된 것에는 X영동대가 사라저 5개의 영동대가 나타났다. 5. 139일된 미성숙계에 estradiol benzoate를 주사한 것에 있어서는 같은 시기에 나타나지 않는 X영동대가 나타나서 산란중의 암탉혈청의 영동대와 같았다. 또한 산란계와 estradiol benzoate로 처리한 암탉과의 component비도 서로 비슷하게 나타났음이 주목 할만하다. 이상과 같은 결과를 종합적으로 고찰하여 볼 때 혈청단백질의 영동상은 암탉이 성장함에 따라 증가하여 산란시기에 가장 많이 나타났으며 산란이 정지되면 점차 감소됨을 알 수 있었다. 그리고 산란시기에는 미지의 단백질성분(x fraction)이 혈청내에 존재한다고 생각되며 이것은 estrogen에 의하여 그 단백질의 생산이 촉진되는 것 같다. The level of total serum protein in female fowl, from embryo to non-laying old hens, has been ascertained by filter paper-electrophoresis. The result of the experiment, showed that the number of bands in the serum protein of laying hens increased with the growth and reached maximum at the staged laying egg, however they decreased in non-laying old hen as follows. The bands of the serum protein of the embryo incubative for 18 days was shown 4. and It made up prealbumin in front of albumin band. The pullets 3, 110, 134 and 148 days old were appeared pre-albumin, globulin-α₂, γ. It was shown that 6 bands appeared in the serum protein of the 173 days old laying hens. In the serum protein of 520 days old non-laying hens, bands decreased from 6 to 5. In the serum protein of 139 days old immatured hens injected estradiol benzoate appeared same bands as that of 173 days old laying hens. In both laying hen and pullets which were injected appeared the same amount of α₁, α₂, γ-globulins and X-component.